Amino acid dipepetide frequency for Tubulinosema ratisbonensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.241AlaAla: 1.241 ± 0.143
0.516AlaCys: 0.516 ± 0.02
1.178AlaAsp: 1.178 ± 0.041
1.881AlaGlu: 1.881 ± 0.068
1.735AlaPhe: 1.735 ± 0.054
1.135AlaGly: 1.135 ± 0.062
0.53AlaHis: 0.53 ± 0.025
2.308AlaIle: 2.308 ± 0.052
2.598AlaLys: 2.598 ± 0.064
3.302AlaLeu: 3.302 ± 0.063
0.643AlaMet: 0.643 ± 0.031
1.639AlaAsn: 1.639 ± 0.048
0.771AlaPro: 0.771 ± 0.038
0.92AlaGln: 0.92 ± 0.041
0.938AlaArg: 0.938 ± 0.037
1.776AlaSer: 1.776 ± 0.053
1.128AlaThr: 1.128 ± 0.037
1.536AlaVal: 1.536 ± 0.04
0.152AlaTrp: 0.152 ± 0.013
1.219AlaTyr: 1.219 ± 0.041
0.074AlaXaa: 0.074 ± 0.01
Cys
0.569CysAla: 0.569 ± 0.026
0.398CysCys: 0.398 ± 0.02
0.99CysAsp: 0.99 ± 0.036
1.142CysGlu: 1.142 ± 0.039
1.378CysPhe: 1.378 ± 0.043
0.724CysGly: 0.724 ± 0.031
0.277CysHis: 0.277 ± 0.019
1.474CysIle: 1.474 ± 0.044
1.921CysLys: 1.921 ± 0.048
2.024CysLeu: 2.024 ± 0.044
0.316CysMet: 0.316 ± 0.021
1.091CysAsn: 1.091 ± 0.038
0.427CysPro: 0.427 ± 0.022
0.387CysGln: 0.387 ± 0.027
0.456CysArg: 0.456 ± 0.022
1.171CysSer: 1.171 ± 0.03
0.898CysThr: 0.898 ± 0.032
1.111CysVal: 1.111 ± 0.038
0.086CysTrp: 0.086 ± 0.01
0.885CysTyr: 0.885 ± 0.034
0.051CysXaa: 0.051 ± 0.008
Asp
1.231AspAla: 1.231 ± 0.052
1.001AspCys: 1.001 ± 0.041
2.766AspAsp: 2.766 ± 0.058
3.834AspGlu: 3.834 ± 0.068
3.954AspPhe: 3.954 ± 0.066
1.429AspGly: 1.429 ± 0.048
0.69AspHis: 0.69 ± 0.031
3.849AspIle: 3.849 ± 0.06
5.141AspLys: 5.141 ± 0.078
6.026AspLeu: 6.026 ± 0.081
0.811AspMet: 0.811 ± 0.026
2.93AspAsn: 2.93 ± 0.067
1.337AspPro: 1.337 ± 0.039
1.322AspGln: 1.322 ± 0.046
1.406AspArg: 1.406 ± 0.045
3.676AspSer: 3.676 ± 0.067
2.364AspThr: 2.364 ± 0.06
2.153AspVal: 2.153 ± 0.049
0.324AspTrp: 0.324 ± 0.019
2.486AspTyr: 2.486 ± 0.052
0.115AspXaa: 0.115 ± 0.012
Glu
1.976GluAla: 1.976 ± 0.069
1.306GluCys: 1.306 ± 0.044
3.718GluAsp: 3.718 ± 0.064
9.005GluGlu: 9.005 ± 0.158
4.171GluPhe: 4.171 ± 0.079
2.395GluGly: 2.395 ± 0.067
0.907GluHis: 0.907 ± 0.031
8.543GluIle: 8.543 ± 0.136
9.698GluLys: 9.698 ± 0.135
7.034GluLeu: 7.034 ± 0.104
1.921GluMet: 1.921 ± 0.048
7.315GluAsn: 7.315 ± 0.107
1.461GluPro: 1.461 ± 0.043
1.775GluGln: 1.775 ± 0.05
2.679GluArg: 2.679 ± 0.064
5.066GluSer: 5.066 ± 0.084
3.18GluThr: 3.18 ± 0.063
3.494GluVal: 3.494 ± 0.062
0.35GluTrp: 0.35 ± 0.017
3.198GluTyr: 3.198 ± 0.065
0.235GluXaa: 0.235 ± 0.019
Phe
1.671PheAla: 1.671 ± 0.047
1.488PheCys: 1.488 ± 0.041
4.046PheAsp: 4.046 ± 0.063
3.804PheGlu: 3.804 ± 0.067
5.467PhePhe: 5.467 ± 0.102
2.19PheGly: 2.19 ± 0.057
0.953PheHis: 0.953 ± 0.032
7.044PheIle: 7.044 ± 0.109
6.072PheLys: 6.072 ± 0.095
9.286PheLeu: 9.286 ± 0.131
1.403PheMet: 1.403 ± 0.04
4.865PheAsn: 4.865 ± 0.082
1.449PhePro: 1.449 ± 0.041
1.3PheGln: 1.3 ± 0.039
1.816PheArg: 1.816 ± 0.047
5.228PheSer: 5.228 ± 0.091
3.615PheThr: 3.615 ± 0.064
3.983PheVal: 3.983 ± 0.074
0.356PheTrp: 0.356 ± 0.018
4.682PheTyr: 4.682 ± 0.08
0.208PheXaa: 0.208 ± 0.019
Gly
1.058GlyAla: 1.058 ± 0.052
0.721GlyCys: 0.721 ± 0.031
1.45GlyAsp: 1.45 ± 0.045
2.061GlyGlu: 2.061 ± 0.059
1.911GlyPhe: 1.911 ± 0.049
1.524GlyGly: 1.524 ± 0.084
0.583GlyHis: 0.583 ± 0.024
3.094GlyIle: 3.094 ± 0.065
3.355GlyLys: 3.355 ± 0.072
3.326GlyLeu: 3.326 ± 0.065
0.853GlyMet: 0.853 ± 0.031
2.185GlyAsn: 2.185 ± 0.054
0.676GlyPro: 0.676 ± 0.032
0.683GlyGln: 0.683 ± 0.03
1.167GlyArg: 1.167 ± 0.042
2.213GlySer: 2.213 ± 0.064
1.646GlyThr: 1.646 ± 0.047
1.859GlyVal: 1.859 ± 0.044
0.216GlyTrp: 0.216 ± 0.016
1.607GlyTyr: 1.607 ± 0.046
0.092GlyXaa: 0.092 ± 0.011
His
0.426HisAla: 0.426 ± 0.021
0.377HisCys: 0.377 ± 0.021
0.572HisAsp: 0.572 ± 0.028
0.995HisGlu: 0.995 ± 0.033
1.348HisPhe: 1.348 ± 0.036
0.562HisGly: 0.562 ± 0.028
0.284HisHis: 0.284 ± 0.019
1.06HisIle: 1.06 ± 0.038
1.725HisLys: 1.725 ± 0.047
1.787HisLeu: 1.787 ± 0.045
0.246HisMet: 0.246 ± 0.015
0.997HisAsn: 0.997 ± 0.03
0.647HisPro: 0.647 ± 0.027
0.413HisGln: 0.413 ± 0.022
0.537HisArg: 0.537 ± 0.024
1.358HisSer: 1.358 ± 0.041
0.712HisThr: 0.712 ± 0.029
0.639HisVal: 0.639 ± 0.027
0.091HisTrp: 0.091 ± 0.01
0.656HisTyr: 0.656 ± 0.028
0.045HisXaa: 0.045 ± 0.008
Ile
2.194IleAla: 2.194 ± 0.055
1.758IleCys: 1.758 ± 0.043
4.267IleAsp: 4.267 ± 0.067
5.722IleGlu: 5.722 ± 0.079
7.35IlePhe: 7.35 ± 0.109
2.719IleGly: 2.719 ± 0.06
1.552IleHis: 1.552 ± 0.045
7.54IleIle: 7.54 ± 0.106
10.513IleLys: 10.513 ± 0.141
10.585IleLeu: 10.585 ± 0.13
1.255IleMet: 1.255 ± 0.037
7.642IleAsn: 7.642 ± 0.122
2.703IlePro: 2.703 ± 0.058
2.581IleGln: 2.581 ± 0.051
2.605IleArg: 2.605 ± 0.054
6.908IleSer: 6.908 ± 0.084
4.072IleThr: 4.072 ± 0.068
3.681IleVal: 3.681 ± 0.067
0.443IleTrp: 0.443 ± 0.022
4.371IleTyr: 4.371 ± 0.08
0.2IleXaa: 0.2 ± 0.016
Lys
2.656LysAla: 2.656 ± 0.06
1.616LysCys: 1.616 ± 0.045
5.308LysAsp: 5.308 ± 0.079
12.672LysGlu: 12.672 ± 0.16
5.395LysPhe: 5.395 ± 0.09
3.219LysGly: 3.219 ± 0.071
1.622LysHis: 1.622 ± 0.038
11.564LysIle: 11.564 ± 0.122
13.475LysLys: 13.475 ± 0.153
9.588LysLeu: 9.588 ± 0.111
2.591LysMet: 2.591 ± 0.045
10.049LysAsn: 10.049 ± 0.115
2.445LysPro: 2.445 ± 0.07
2.718LysGln: 2.718 ± 0.058
4.154LysArg: 4.154 ± 0.064
6.573LysSer: 6.573 ± 0.097
4.771LysThr: 4.771 ± 0.074
4.849LysVal: 4.849 ± 0.082
0.486LysTrp: 0.486 ± 0.025
4.601LysTyr: 4.601 ± 0.078
0.305LysXaa: 0.305 ± 0.022
Leu
3.296LeuAla: 3.296 ± 0.061
1.927LeuCys: 1.927 ± 0.044
4.523LeuAsp: 4.523 ± 0.073
8.387LeuGlu: 8.387 ± 0.122
8.013LeuPhe: 8.013 ± 0.124
3.49LeuGly: 3.49 ± 0.073
1.625LeuHis: 1.625 ± 0.044
10.459LeuIle: 10.459 ± 0.143
12.419LeuLys: 12.419 ± 0.15
10.979LeuLeu: 10.979 ± 0.135
1.732LeuMet: 1.732 ± 0.048
7.857LeuAsn: 7.857 ± 0.103
3.091LeuPro: 3.091 ± 0.058
3.242LeuGln: 3.242 ± 0.053
3.522LeuArg: 3.522 ± 0.064
7.607LeuSer: 7.607 ± 0.095
5.419LeuThr: 5.419 ± 0.08
5.378LeuVal: 5.378 ± 0.093
0.473LeuTrp: 0.473 ± 0.024
3.831LeuTyr: 3.831 ± 0.079
0.255LeuXaa: 0.255 ± 0.019
Met
0.697MetAla: 0.697 ± 0.029
0.327MetCys: 0.327 ± 0.019
0.895MetAsp: 0.895 ± 0.03
1.014MetGlu: 1.014 ± 0.036
1.304MetPhe: 1.304 ± 0.04
0.587MetGly: 0.587 ± 0.024
0.435MetHis: 0.435 ± 0.02
1.89MetIle: 1.89 ± 0.047
2.044MetLys: 2.044 ± 0.043
1.949MetLeu: 1.949 ± 0.047
0.353MetMet: 0.353 ± 0.02
1.616MetAsn: 1.616 ± 0.048
0.554MetPro: 0.554 ± 0.024
0.572MetGln: 0.572 ± 0.024
0.576MetArg: 0.576 ± 0.026
1.294MetSer: 1.294 ± 0.039
0.866MetThr: 0.866 ± 0.032
0.994MetVal: 0.994 ± 0.038
0.06MetTrp: 0.06 ± 0.008
0.709MetTyr: 0.709 ± 0.026
0.052MetXaa: 0.052 ± 0.008
Asn
1.665AsnAla: 1.665 ± 0.051
1.205AsnCys: 1.205 ± 0.039
4.088AsnAsp: 4.088 ± 0.068
6.874AsnGlu: 6.874 ± 0.121
5.837AsnPhe: 5.837 ± 0.104
2.087AsnGly: 2.087 ± 0.049
1.232AsnHis: 1.232 ± 0.04
6.186AsnIle: 6.186 ± 0.103
8.609AsnLys: 8.609 ± 0.121
9.482AsnLeu: 9.482 ± 0.132
1.36AsnMet: 1.36 ± 0.032
5.947AsnAsn: 5.947 ± 0.112
2.058AsnPro: 2.058 ± 0.056
2.583AsnGln: 2.583 ± 0.057
1.791AsnArg: 1.791 ± 0.041
4.926AsnSer: 4.926 ± 0.076
3.718AsnThr: 3.718 ± 0.067
3.358AsnVal: 3.358 ± 0.064
0.339AsnTrp: 0.339 ± 0.018
3.637AsnTyr: 3.637 ± 0.068
0.215AsnXaa: 0.215 ± 0.017
Pro
0.721ProAla: 0.721 ± 0.041
0.452ProCys: 0.452 ± 0.025
1.11ProAsp: 1.11 ± 0.038
2.109ProGlu: 2.109 ± 0.05
1.836ProPhe: 1.836 ± 0.046
0.965ProGly: 0.965 ± 0.034
0.471ProHis: 0.471 ± 0.022
2.199ProIle: 2.199 ± 0.059
2.673ProLys: 2.673 ± 0.062
2.534ProLeu: 2.534 ± 0.057
0.436ProMet: 0.436 ± 0.022
1.804ProAsn: 1.804 ± 0.056
0.893ProPro: 0.893 ± 0.056
0.886ProGln: 0.886 ± 0.036
0.792ProArg: 0.792 ± 0.033
2.198ProSer: 2.198 ± 0.052
1.32ProThr: 1.32 ± 0.046
1.518ProVal: 1.518 ± 0.05
0.114ProTrp: 0.114 ± 0.011
0.955ProTyr: 0.955 ± 0.032
0.079ProXaa: 0.079 ± 0.009
Gln
0.822GlnAla: 0.822 ± 0.039
0.423GlnCys: 0.423 ± 0.024
1.084GlnAsp: 1.084 ± 0.036
2.233GlnGlu: 2.233 ± 0.057
1.406GlnPhe: 1.406 ± 0.042
0.84GlnGly: 0.84 ± 0.032
0.332GlnHis: 0.332 ± 0.02
2.818GlnIle: 2.818 ± 0.058
3.158GlnLys: 3.158 ± 0.06
2.438GlnLeu: 2.438 ± 0.052
0.575GlnMet: 0.575 ± 0.023
2.608GlnAsn: 2.608 ± 0.059
0.939GlnPro: 0.939 ± 0.046
0.886GlnGln: 0.886 ± 0.035
1.023GlnArg: 1.023 ± 0.031
2.135GlnSer: 2.135 ± 0.05
1.572GlnThr: 1.572 ± 0.043
1.118GlnVal: 1.118 ± 0.036
0.112GlnTrp: 0.112 ± 0.012
0.759GlnTyr: 0.759 ± 0.027
0.068GlnXaa: 0.068 ± 0.009
Arg
0.931ArgAla: 0.931 ± 0.039
0.469ArgCys: 0.469 ± 0.024
1.295ArgAsp: 1.295 ± 0.042
2.275ArgGlu: 2.275 ± 0.052
1.804ArgPhe: 1.804 ± 0.043
1.096ArgGly: 1.096 ± 0.038
0.468ArgHis: 0.468 ± 0.022
3.122ArgIle: 3.122 ± 0.059
3.922ArgLys: 3.922 ± 0.074
2.7ArgLeu: 2.7 ± 0.059
0.854ArgMet: 0.854 ± 0.036
2.758ArgAsn: 2.758 ± 0.063
0.707ArgPro: 0.707 ± 0.03
0.708ArgGln: 0.708 ± 0.029
1.58ArgArg: 1.58 ± 0.052
1.995ArgSer: 1.995 ± 0.043
1.315ArgThr: 1.315 ± 0.039
1.529ArgVal: 1.529 ± 0.04
0.158ArgTrp: 0.158 ± 0.011
1.16ArgTyr: 1.16 ± 0.039
0.079ArgXaa: 0.079 ± 0.01
Ser
2.016SerAla: 2.016 ± 0.069
1.047SerCys: 1.047 ± 0.037
4.043SerAsp: 4.043 ± 0.065
5.117SerGlu: 5.117 ± 0.085
5.951SerPhe: 5.951 ± 0.088
2.284SerGly: 2.284 ± 0.062
1.089SerHis: 1.089 ± 0.037
5.726SerIle: 5.726 ± 0.075
7.194SerLys: 7.194 ± 0.088
8.099SerLeu: 8.099 ± 0.11
1.133SerMet: 1.133 ± 0.039
4.62SerAsn: 4.62 ± 0.076
1.678SerPro: 1.678 ± 0.049
2.141SerGln: 2.141 ± 0.046
1.757SerArg: 1.757 ± 0.053
5.341SerSer: 5.341 ± 0.137
3.314SerThr: 3.314 ± 0.069
3.929SerVal: 3.929 ± 0.065
0.294SerTrp: 0.294 ± 0.018
2.927SerTyr: 2.927 ± 0.057
0.185SerXaa: 0.185 ± 0.015
Thr
1.327ThrAla: 1.327 ± 0.04
0.698ThrCys: 0.698 ± 0.025
2.381ThrAsp: 2.381 ± 0.047
3.368ThrGlu: 3.368 ± 0.062
3.341ThrPhe: 3.341 ± 0.056
1.793ThrGly: 1.793 ± 0.049
0.785ThrHis: 0.785 ± 0.028
3.715ThrIle: 3.715 ± 0.071
5.126ThrLys: 5.126 ± 0.085
4.905ThrLeu: 4.905 ± 0.08
0.641ThrMet: 0.641 ± 0.026
4.059ThrAsn: 4.059 ± 0.074
1.669ThrPro: 1.669 ± 0.057
1.562ThrGln: 1.562 ± 0.041
1.296ThrArg: 1.296 ± 0.038
3.225ThrSer: 3.225 ± 0.069
2.444ThrThr: 2.444 ± 0.072
2.177ThrVal: 2.177 ± 0.05
0.232ThrTrp: 0.232 ± 0.015
1.772ThrTyr: 1.772 ± 0.041
0.15ThrXaa: 0.15 ± 0.015
Val
1.295ValAla: 1.295 ± 0.036
1.102ValCys: 1.102 ± 0.029
2.339ValAsp: 2.339 ± 0.054
3.229ValGlu: 3.229 ± 0.058
3.5ValPhe: 3.5 ± 0.065
1.637ValGly: 1.637 ± 0.044
0.775ValHis: 0.775 ± 0.03
4.442ValIle: 4.442 ± 0.073
5.283ValLys: 5.283 ± 0.075
5.176ValLeu: 5.176 ± 0.081
0.836ValMet: 0.836 ± 0.029
3.68ValAsn: 3.68 ± 0.071
1.352ValPro: 1.352 ± 0.049
1.148ValGln: 1.148 ± 0.035
1.432ValArg: 1.432 ± 0.042
3.475ValSer: 3.475 ± 0.065
2.01ValThr: 2.01 ± 0.046
2.438ValVal: 2.438 ± 0.056
0.247ValTrp: 0.247 ± 0.014
2.595ValTyr: 2.595 ± 0.049
0.121ValXaa: 0.121 ± 0.012
Trp
0.183TrpAla: 0.183 ± 0.015
0.099TrpCys: 0.099 ± 0.011
0.277TrpAsp: 0.277 ± 0.02
0.29TrpGlu: 0.29 ± 0.019
0.349TrpPhe: 0.349 ± 0.022
0.159TrpGly: 0.159 ± 0.014
0.083TrpHis: 0.083 ± 0.01
0.423TrpIle: 0.423 ± 0.021
0.408TrpLys: 0.408 ± 0.022
0.514TrpLeu: 0.514 ± 0.024
0.132TrpMet: 0.132 ± 0.012
0.345TrpAsn: 0.345 ± 0.017
0.105TrpPro: 0.105 ± 0.01
0.139TrpGln: 0.139 ± 0.012
0.207TrpArg: 0.207 ± 0.015
0.357TrpSer: 0.357 ± 0.02
0.201TrpThr: 0.201 ± 0.014
0.247TrpVal: 0.247 ± 0.018
0.034TrpTrp: 0.034 ± 0.007
0.262TrpTyr: 0.262 ± 0.017
0.018TrpXaa: 0.018 ± 0.005
Tyr
1.178TyrAla: 1.178 ± 0.039
0.796TyrCys: 0.796 ± 0.028
2.396TyrAsp: 2.396 ± 0.053
3.196TyrGlu: 3.196 ± 0.054
4.695TyrPhe: 4.695 ± 0.092
1.348TyrGly: 1.348 ± 0.041
0.751TyrHis: 0.751 ± 0.03
2.984TyrIle: 2.984 ± 0.062
4.974TyrLys: 4.974 ± 0.086
5.55TyrLeu: 5.55 ± 0.075
0.625TyrMet: 0.625 ± 0.027
2.958TyrAsn: 2.958 ± 0.058
1.039TyrPro: 1.039 ± 0.035
1.31TyrGln: 1.31 ± 0.036
1.103TyrArg: 1.103 ± 0.034
3.201TyrSer: 3.201 ± 0.059
1.971TyrThr: 1.971 ± 0.048
1.97TyrVal: 1.97 ± 0.046
0.249TyrTrp: 0.249 ± 0.017
2.028TyrTyr: 2.028 ± 0.054
0.108TyrXaa: 0.108 ± 0.011
Xaa
0.063XaaAla: 0.063 ± 0.009
0.052XaaCys: 0.052 ± 0.008
0.116XaaAsp: 0.116 ± 0.012
0.22XaaGlu: 0.22 ± 0.021
0.178XaaPhe: 0.178 ± 0.017
0.098XaaGly: 0.098 ± 0.012
0.034XaaHis: 0.034 ± 0.006
0.22XaaIle: 0.22 ± 0.018
0.349XaaLys: 0.349 ± 0.022
0.248XaaLeu: 0.248 ± 0.016
0.073XaaMet: 0.073 ± 0.009
0.233XaaAsn: 0.233 ± 0.018
0.063XaaPro: 0.063 ± 0.008
0.072XaaGln: 0.072 ± 0.008
0.086XaaArg: 0.086 ± 0.011
0.16XaaSer: 0.16 ± 0.011
0.131XaaThr: 0.131 ± 0.013
0.151XaaVal: 0.151 ± 0.013
0.017XaaTrp: 0.017 ± 0.005
0.09XaaTyr: 0.09 ± 0.009
2.313XaaXaa: 2.313 ± 0.277
Statistics based on 2996 proteins (936452 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski