Halorubrum phage HF2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Haloferacalesvirus; Halorubrum virus HF2

Average proteome isoelectric point is 5.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 126 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8V6T4|Q8V6T4_9CAUD Uncharacterized protein OS=Halorubrum phage HF2 OX=33771 GN=HrrHF2_160 PE=4 SV=1
MM1 pKa = 7.1STLADD6 pKa = 4.76FGADD10 pKa = 3.3VPEE13 pKa = 4.44EE14 pKa = 3.77AWKK17 pKa = 10.84DD18 pKa = 3.17RR19 pKa = 11.84DD20 pKa = 3.72TLYY23 pKa = 11.03EE24 pKa = 4.32LYY26 pKa = 10.45HH27 pKa = 5.78EE28 pKa = 4.62QGLDD32 pKa = 3.3QSEE35 pKa = 4.3MGDD38 pKa = 3.63EE39 pKa = 4.63LGCSAGTISYY49 pKa = 9.83WMDD52 pKa = 3.04KK53 pKa = 10.86LGVDD57 pKa = 3.95TTHH60 pKa = 7.09TSHH63 pKa = 6.19NTQAEE68 pKa = 4.24PQPEE72 pKa = 4.08DD73 pKa = 3.33WDD75 pKa = 4.09CEE77 pKa = 4.16YY78 pKa = 11.36HH79 pKa = 6.05EE80 pKa = 4.61VCGNEE85 pKa = 4.09TPGPRR90 pKa = 11.84NGVCDD95 pKa = 3.55RR96 pKa = 11.84CLDD99 pKa = 4.11LARR102 pKa = 11.84LNQSGYY108 pKa = 10.93YY109 pKa = 10.21DD110 pKa = 4.3GEE112 pKa = 4.26TADD115 pKa = 3.97RR116 pKa = 11.84SSFDD120 pKa = 3.35EE121 pKa = 3.95MSEE124 pKa = 4.19FMAALYY130 pKa = 9.65DD131 pKa = 3.51IHH133 pKa = 8.05GKK135 pKa = 10.4DD136 pKa = 3.84SEE138 pKa = 4.27NN139 pKa = 3.29

Molecular weight: 15.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9PN76|A0A6B9PN76_9CAUD Putative CxxC motif protein OS=Halorubrum phage HF2 OX=33771 GN=HrrHF2_095 PE=4 SV=1
MM1 pKa = 7.37SEE3 pKa = 4.06LMAGLMVFGAVTITTLVLLPVFMLQKK29 pKa = 9.53MASEE33 pKa = 4.37EE34 pKa = 4.15RR35 pKa = 11.84NKK37 pKa = 10.3NHH39 pKa = 5.33QKK41 pKa = 10.64KK42 pKa = 8.43MKK44 pKa = 9.54EE45 pKa = 3.75VAEE48 pKa = 4.12RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84LEE53 pKa = 3.88QNKK56 pKa = 9.2KK57 pKa = 9.84RR58 pKa = 11.84RR59 pKa = 11.84SS60 pKa = 3.43

Molecular weight: 7.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

126

0

126

23576

29

1288

187.1

20.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.693 ± 0.244

0.908 ± 0.11

8.844 ± 0.303

9.756 ± 0.298

3.733 ± 0.171

8.055 ± 0.441

2.227 ± 0.15

4.725 ± 0.172

4.267 ± 0.195

6.952 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.346 ± 0.145

4.475 ± 0.153

4.445 ± 0.158

2.939 ± 0.129

5.582 ± 0.201

6.57 ± 0.269

5.607 ± 0.205

6.808 ± 0.181

1.37 ± 0.096

3.699 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski