Amino acid dipepetide frequency for Halorubrum phage HF2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.327AlaAla: 4.327 ± 0.45
0.509AlaCys: 0.509 ± 0.165
5.048AlaAsp: 5.048 ± 0.434
6.32AlaGlu: 6.32 ± 0.557
2.672AlaPhe: 2.672 ± 0.306
5.514AlaGly: 5.514 ± 0.571
1.485AlaHis: 1.485 ± 0.246
2.63AlaIle: 2.63 ± 0.284
3.606AlaLys: 3.606 ± 0.429
5.472AlaLeu: 5.472 ± 0.579
1.357AlaMet: 1.357 ± 0.251
3.012AlaAsn: 3.012 ± 0.361
3.054AlaPro: 3.054 ± 0.366
2.163AlaGln: 2.163 ± 0.327
3.436AlaArg: 3.436 ± 0.434
4.496AlaSer: 4.496 ± 0.383
3.945AlaThr: 3.945 ± 0.455
4.581AlaVal: 4.581 ± 0.529
0.594AlaTrp: 0.594 ± 0.156
2.715AlaTyr: 2.715 ± 0.335
0.0AlaXaa: 0.0 ± 0.0
Cys
0.382CysAla: 0.382 ± 0.142
0.127CysCys: 0.127 ± 0.098
0.764CysAsp: 0.764 ± 0.204
0.976CysGlu: 0.976 ± 0.235
0.212CysPhe: 0.212 ± 0.105
1.23CysGly: 1.23 ± 0.234
0.297CysHis: 0.297 ± 0.133
0.127CysIle: 0.127 ± 0.069
0.382CysLys: 0.382 ± 0.131
0.594CysLeu: 0.594 ± 0.171
0.042CysMet: 0.042 ± 0.039
0.424CysAsn: 0.424 ± 0.137
0.933CysPro: 0.933 ± 0.254
0.297CysGln: 0.297 ± 0.1
0.679CysArg: 0.679 ± 0.172
0.551CysSer: 0.551 ± 0.179
0.339CysThr: 0.339 ± 0.108
0.382CysVal: 0.382 ± 0.136
0.17CysTrp: 0.17 ± 0.084
0.17CysTyr: 0.17 ± 0.074
0.0CysXaa: 0.0 ± 0.0
Asp
6.363AspAla: 6.363 ± 0.586
0.976AspCys: 0.976 ± 0.192
7.72AspAsp: 7.72 ± 0.778
9.417AspGlu: 9.417 ± 0.753
4.411AspPhe: 4.411 ± 0.449
8.399AspGly: 8.399 ± 0.735
1.569AspHis: 1.569 ± 0.304
3.69AspIle: 3.69 ± 0.443
2.163AspLys: 2.163 ± 0.333
6.66AspLeu: 6.66 ± 0.474
1.145AspMet: 1.145 ± 0.192
2.587AspAsn: 2.587 ± 0.383
4.072AspPro: 4.072 ± 0.503
1.442AspGln: 1.442 ± 0.175
3.86AspArg: 3.86 ± 0.371
6.235AspSer: 6.235 ± 0.447
4.666AspThr: 4.666 ± 0.514
7.041AspVal: 7.041 ± 0.438
1.4AspTrp: 1.4 ± 0.208
4.624AspTyr: 4.624 ± 0.47
0.0AspXaa: 0.0 ± 0.0
Glu
6.829GluAla: 6.829 ± 0.541
0.636GluCys: 0.636 ± 0.179
9.459GluAsp: 9.459 ± 0.606
8.526GluGlu: 8.526 ± 0.691
3.606GluPhe: 3.606 ± 0.428
6.872GluGly: 6.872 ± 0.499
1.994GluHis: 1.994 ± 0.29
5.769GluIle: 5.769 ± 0.452
4.327GluLys: 4.327 ± 0.457
7.296GluLeu: 7.296 ± 0.557
2.927GluMet: 2.927 ± 0.486
4.496GluAsn: 4.496 ± 0.505
4.157GluPro: 4.157 ± 0.414
3.478GluGln: 3.478 ± 0.332
5.429GluArg: 5.429 ± 0.494
5.133GluSer: 5.133 ± 0.529
4.963GluThr: 4.963 ± 0.431
6.278GluVal: 6.278 ± 0.573
1.485GluTrp: 1.485 ± 0.238
3.86GluTyr: 3.86 ± 0.386
0.0GluXaa: 0.0 ± 0.0
Phe
2.206PheAla: 2.206 ± 0.348
0.339PheCys: 0.339 ± 0.111
3.987PheAsp: 3.987 ± 0.398
3.902PheGlu: 3.902 ± 0.33
1.23PhePhe: 1.23 ± 0.255
3.351PheGly: 3.351 ± 0.326
0.848PheHis: 0.848 ± 0.182
1.866PheIle: 1.866 ± 0.302
1.485PheLys: 1.485 ± 0.211
2.121PheLeu: 2.121 ± 0.301
1.103PheMet: 1.103 ± 0.226
1.951PheAsn: 1.951 ± 0.276
1.569PhePro: 1.569 ± 0.231
1.06PheGln: 1.06 ± 0.214
1.909PheArg: 1.909 ± 0.263
2.884PheSer: 2.884 ± 0.338
1.782PheThr: 1.782 ± 0.251
1.951PheVal: 1.951 ± 0.362
0.297PheTrp: 0.297 ± 0.109
1.485PheTyr: 1.485 ± 0.263
0.0PheXaa: 0.0 ± 0.0
Gly
5.133GlyAla: 5.133 ± 0.742
0.551GlyCys: 0.551 ± 0.149
7.678GlyAsp: 7.678 ± 0.616
7.635GlyGlu: 7.635 ± 0.563
2.969GlyPhe: 2.969 ± 0.314
9.926GlyGly: 9.926 ± 2.238
1.782GlyHis: 1.782 ± 0.243
3.224GlyIle: 3.224 ± 0.342
3.181GlyLys: 3.181 ± 0.319
4.284GlyLeu: 4.284 ± 0.429
1.569GlyMet: 1.569 ± 0.209
3.902GlyAsn: 3.902 ± 0.559
2.078GlyPro: 2.078 ± 0.266
2.46GlyGln: 2.46 ± 0.264
4.793GlyArg: 4.793 ± 0.482
5.557GlySer: 5.557 ± 0.7
3.987GlyThr: 3.987 ± 0.403
5.345GlyVal: 5.345 ± 0.47
1.23GlyTrp: 1.23 ± 0.242
3.266GlyTyr: 3.266 ± 0.373
0.0GlyXaa: 0.0 ± 0.0
His
1.188HisAla: 1.188 ± 0.25
0.085HisCys: 0.085 ± 0.061
1.994HisAsp: 1.994 ± 0.259
1.697HisGlu: 1.697 ± 0.288
0.891HisPhe: 0.891 ± 0.209
2.291HisGly: 2.291 ± 0.343
0.679HisHis: 0.679 ± 0.21
1.569HisIle: 1.569 ± 0.314
0.764HisLys: 0.764 ± 0.16
1.697HisLeu: 1.697 ± 0.254
0.297HisMet: 0.297 ± 0.13
1.145HisAsn: 1.145 ± 0.222
1.315HisPro: 1.315 ± 0.224
0.424HisGln: 0.424 ± 0.127
0.891HisArg: 0.891 ± 0.241
1.357HisSer: 1.357 ± 0.24
1.145HisThr: 1.145 ± 0.219
1.315HisVal: 1.315 ± 0.316
0.212HisTrp: 0.212 ± 0.092
1.315HisTyr: 1.315 ± 0.256
0.0HisXaa: 0.0 ± 0.0
Ile
2.927IleAla: 2.927 ± 0.369
0.297IleCys: 0.297 ± 0.111
4.327IleAsp: 4.327 ± 0.447
7.466IleGlu: 7.466 ± 0.51
1.018IlePhe: 1.018 ± 0.257
2.757IleGly: 2.757 ± 0.337
1.315IleHis: 1.315 ± 0.285
2.291IleIle: 2.291 ± 0.275
1.697IleLys: 1.697 ± 0.258
3.012IleLeu: 3.012 ± 0.403
1.06IleMet: 1.06 ± 0.194
1.824IleAsn: 1.824 ± 0.272
2.333IlePro: 2.333 ± 0.315
1.315IleGln: 1.315 ± 0.194
3.012IleArg: 3.012 ± 0.429
3.436IleSer: 3.436 ± 0.512
2.842IleThr: 2.842 ± 0.28
2.969IleVal: 2.969 ± 0.35
0.297IleTrp: 0.297 ± 0.091
1.06IleTyr: 1.06 ± 0.195
0.0IleXaa: 0.0 ± 0.0
Lys
2.927LysAla: 2.927 ± 0.389
0.382LysCys: 0.382 ± 0.168
2.672LysAsp: 2.672 ± 0.365
3.733LysGlu: 3.733 ± 0.376
1.782LysPhe: 1.782 ± 0.326
2.757LysGly: 2.757 ± 0.327
0.891LysHis: 0.891 ± 0.209
1.569LysIle: 1.569 ± 0.264
2.078LysLys: 2.078 ± 0.396
3.351LysLeu: 3.351 ± 0.378
1.103LysMet: 1.103 ± 0.199
2.078LysAsn: 2.078 ± 0.329
1.909LysPro: 1.909 ± 0.275
1.527LysGln: 1.527 ± 0.247
3.012LysArg: 3.012 ± 0.328
2.757LysSer: 2.757 ± 0.312
2.715LysThr: 2.715 ± 0.32
2.8LysVal: 2.8 ± 0.366
0.721LysTrp: 0.721 ± 0.195
1.909LysTyr: 1.909 ± 0.297
0.0LysXaa: 0.0 ± 0.0
Leu
5.387LeuAla: 5.387 ± 0.586
0.551LeuCys: 0.551 ± 0.171
6.363LeuAsp: 6.363 ± 0.604
7.041LeuGlu: 7.041 ± 0.534
2.163LeuPhe: 2.163 ± 0.328
4.708LeuGly: 4.708 ± 0.493
1.569LeuHis: 1.569 ± 0.242
3.224LeuIle: 3.224 ± 0.277
3.521LeuLys: 3.521 ± 0.426
4.327LeuLeu: 4.327 ± 0.486
1.994LeuMet: 1.994 ± 0.309
3.012LeuAsn: 3.012 ± 0.46
3.054LeuPro: 3.054 ± 0.318
1.739LeuGln: 1.739 ± 0.235
4.157LeuArg: 4.157 ± 0.415
5.005LeuSer: 5.005 ± 0.476
4.115LeuThr: 4.115 ± 0.373
4.369LeuVal: 4.369 ± 0.431
0.848LeuTrp: 0.848 ± 0.192
2.375LeuTyr: 2.375 ± 0.347
0.0LeuXaa: 0.0 ± 0.0
Met
2.121MetAla: 2.121 ± 0.415
0.255MetCys: 0.255 ± 0.099
2.036MetAsp: 2.036 ± 0.256
2.248MetGlu: 2.248 ± 0.319
0.764MetPhe: 0.764 ± 0.218
1.485MetGly: 1.485 ± 0.266
0.339MetHis: 0.339 ± 0.13
0.891MetIle: 0.891 ± 0.165
1.527MetLys: 1.527 ± 0.268
1.4MetLeu: 1.4 ± 0.256
0.721MetMet: 0.721 ± 0.177
1.569MetAsn: 1.569 ± 0.306
0.848MetPro: 0.848 ± 0.172
0.339MetGln: 0.339 ± 0.112
1.018MetArg: 1.018 ± 0.223
2.418MetSer: 2.418 ± 0.32
1.357MetThr: 1.357 ± 0.239
1.315MetVal: 1.315 ± 0.231
0.339MetTrp: 0.339 ± 0.105
0.467MetTyr: 0.467 ± 0.159
0.0MetXaa: 0.0 ± 0.0
Asn
2.969AsnAla: 2.969 ± 0.288
0.551AsnCys: 0.551 ± 0.179
3.097AsnAsp: 3.097 ± 0.406
3.987AsnGlu: 3.987 ± 0.362
1.951AsnPhe: 1.951 ± 0.292
4.454AsnGly: 4.454 ± 0.717
1.018AsnHis: 1.018 ± 0.209
2.248AsnIle: 2.248 ± 0.386
1.697AsnLys: 1.697 ± 0.305
2.587AsnLeu: 2.587 ± 0.366
0.467AsnMet: 0.467 ± 0.127
2.078AsnAsn: 2.078 ± 0.421
2.715AsnPro: 2.715 ± 0.316
1.485AsnGln: 1.485 ± 0.281
3.266AsnArg: 3.266 ± 0.36
3.266AsnSer: 3.266 ± 0.48
2.333AsnThr: 2.333 ± 0.334
2.375AsnVal: 2.375 ± 0.308
0.467AsnTrp: 0.467 ± 0.161
1.739AsnTyr: 1.739 ± 0.294
0.0AsnXaa: 0.0 ± 0.0
Pro
3.012ProAla: 3.012 ± 0.279
0.509ProCys: 0.509 ± 0.153
5.387ProAsp: 5.387 ± 0.425
5.005ProGlu: 5.005 ± 0.484
1.697ProPhe: 1.697 ± 0.339
2.63ProGly: 2.63 ± 0.301
1.018ProHis: 1.018 ± 0.217
2.163ProIle: 2.163 ± 0.335
2.036ProLys: 2.036 ± 0.302
2.63ProLeu: 2.63 ± 0.359
1.06ProMet: 1.06 ± 0.222
1.569ProAsn: 1.569 ± 0.235
2.248ProPro: 2.248 ± 0.312
0.891ProGln: 0.891 ± 0.224
1.909ProArg: 1.909 ± 0.337
2.503ProSer: 2.503 ± 0.361
2.757ProThr: 2.757 ± 0.375
3.648ProVal: 3.648 ± 0.347
0.806ProTrp: 0.806 ± 0.177
0.976ProTyr: 0.976 ± 0.234
0.0ProXaa: 0.0 ± 0.0
Gln
2.036GlnAla: 2.036 ± 0.334
0.212GlnCys: 0.212 ± 0.108
1.527GlnAsp: 1.527 ± 0.308
2.206GlnGlu: 2.206 ± 0.32
0.933GlnPhe: 0.933 ± 0.197
1.782GlnGly: 1.782 ± 0.255
1.06GlnHis: 1.06 ± 0.22
1.4GlnIle: 1.4 ± 0.257
1.866GlnLys: 1.866 ± 0.283
2.927GlnLeu: 2.927 ± 0.361
1.315GlnMet: 1.315 ± 0.315
1.527GlnAsn: 1.527 ± 0.281
0.933GlnPro: 0.933 ± 0.213
1.018GlnGln: 1.018 ± 0.21
1.569GlnArg: 1.569 ± 0.231
1.824GlnSer: 1.824 ± 0.278
2.078GlnThr: 2.078 ± 0.254
2.078GlnVal: 2.078 ± 0.328
0.297GlnTrp: 0.297 ± 0.131
0.806GlnTyr: 0.806 ± 0.177
0.0GlnXaa: 0.0 ± 0.0
Arg
3.181ArgAla: 3.181 ± 0.348
0.594ArgCys: 0.594 ± 0.15
4.454ArgAsp: 4.454 ± 0.439
5.005ArgGlu: 5.005 ± 0.536
2.206ArgPhe: 2.206 ± 0.278
3.945ArgGly: 3.945 ± 0.41
0.891ArgHis: 0.891 ± 0.202
3.393ArgIle: 3.393 ± 0.382
2.672ArgLys: 2.672 ± 0.314
3.818ArgLeu: 3.818 ± 0.447
1.569ArgMet: 1.569 ± 0.32
3.181ArgAsn: 3.181 ± 0.352
2.291ArgPro: 2.291 ± 0.314
1.824ArgGln: 1.824 ± 0.306
3.945ArgArg: 3.945 ± 0.488
3.351ArgSer: 3.351 ± 0.337
2.206ArgThr: 2.206 ± 0.304
4.03ArgVal: 4.03 ± 0.426
0.848ArgTrp: 0.848 ± 0.191
2.418ArgTyr: 2.418 ± 0.317
0.0ArgXaa: 0.0 ± 0.0
Ser
4.157SerAla: 4.157 ± 0.377
0.636SerCys: 0.636 ± 0.171
5.811SerAsp: 5.811 ± 0.607
6.66SerGlu: 6.66 ± 0.664
2.291SerPhe: 2.291 ± 0.23
6.405SerGly: 6.405 ± 0.725
1.357SerHis: 1.357 ± 0.27
3.224SerIle: 3.224 ± 0.316
2.163SerLys: 2.163 ± 0.324
4.92SerLeu: 4.92 ± 0.445
1.824SerMet: 1.824 ± 0.273
2.842SerAsn: 2.842 ± 0.392
2.333SerPro: 2.333 ± 0.316
1.697SerGln: 1.697 ± 0.267
3.648SerArg: 3.648 ± 0.304
3.987SerSer: 3.987 ± 0.508
3.563SerThr: 3.563 ± 0.501
4.539SerVal: 4.539 ± 0.461
1.23SerTrp: 1.23 ± 0.24
2.418SerTyr: 2.418 ± 0.326
0.0SerXaa: 0.0 ± 0.0
Thr
3.436ThrAla: 3.436 ± 0.368
0.339ThrCys: 0.339 ± 0.135
4.878ThrAsp: 4.878 ± 0.561
4.751ThrGlu: 4.751 ± 0.437
2.248ThrPhe: 2.248 ± 0.31
4.496ThrGly: 4.496 ± 0.438
1.654ThrHis: 1.654 ± 0.317
3.054ThrIle: 3.054 ± 0.339
2.503ThrLys: 2.503 ± 0.312
4.793ThrLeu: 4.793 ± 0.449
1.103ThrMet: 1.103 ± 0.271
1.994ThrAsn: 1.994 ± 0.348
3.012ThrPro: 3.012 ± 0.333
1.739ThrGln: 1.739 ± 0.248
2.375ThrArg: 2.375 ± 0.291
3.054ThrSer: 3.054 ± 0.476
3.945ThrThr: 3.945 ± 0.463
4.284ThrVal: 4.284 ± 0.445
0.848ThrTrp: 0.848 ± 0.159
1.569ThrTyr: 1.569 ± 0.236
0.0ThrXaa: 0.0 ± 0.0
Val
4.878ValAla: 4.878 ± 0.557
0.679ValCys: 0.679 ± 0.195
6.32ValAsp: 6.32 ± 0.523
5.938ValGlu: 5.938 ± 0.533
2.163ValPhe: 2.163 ± 0.299
3.945ValGly: 3.945 ± 0.311
1.4ValHis: 1.4 ± 0.278
3.224ValIle: 3.224 ± 0.332
3.139ValLys: 3.139 ± 0.287
3.987ValLeu: 3.987 ± 0.401
1.527ValMet: 1.527 ± 0.258
3.181ValAsn: 3.181 ± 0.338
3.266ValPro: 3.266 ± 0.371
2.757ValGln: 2.757 ± 0.323
3.818ValArg: 3.818 ± 0.346
4.878ValSer: 4.878 ± 0.471
4.963ValThr: 4.963 ± 0.489
5.472ValVal: 5.472 ± 0.482
0.721ValTrp: 0.721 ± 0.19
1.824ValTyr: 1.824 ± 0.309
0.0ValXaa: 0.0 ± 0.0
Trp
0.976TrpAla: 0.976 ± 0.181
0.212TrpCys: 0.212 ± 0.108
1.018TrpAsp: 1.018 ± 0.174
1.527TrpGlu: 1.527 ± 0.26
0.764TrpPhe: 0.764 ± 0.166
0.636TrpGly: 0.636 ± 0.149
0.127TrpHis: 0.127 ± 0.078
0.339TrpIle: 0.339 ± 0.134
0.848TrpLys: 0.848 ± 0.204
1.23TrpLeu: 1.23 ± 0.314
0.594TrpMet: 0.594 ± 0.181
0.679TrpAsn: 0.679 ± 0.17
0.424TrpPro: 0.424 ± 0.167
0.382TrpGln: 0.382 ± 0.104
0.976TrpArg: 0.976 ± 0.198
1.018TrpSer: 1.018 ± 0.212
0.467TrpThr: 0.467 ± 0.116
0.636TrpVal: 0.636 ± 0.174
0.255TrpTrp: 0.255 ± 0.1
0.594TrpTyr: 0.594 ± 0.184
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.503TyrAla: 2.503 ± 0.298
0.636TyrCys: 0.636 ± 0.172
3.902TyrAsp: 3.902 ± 0.437
3.521TyrGlu: 3.521 ± 0.406
1.357TyrPhe: 1.357 ± 0.228
2.969TyrGly: 2.969 ± 0.347
0.976TyrHis: 0.976 ± 0.226
1.357TyrIle: 1.357 ± 0.278
1.018TyrLys: 1.018 ± 0.153
2.418TyrLeu: 2.418 ± 0.263
0.636TyrMet: 0.636 ± 0.166
1.697TyrAsn: 1.697 ± 0.278
1.909TyrPro: 1.909 ± 0.282
1.357TyrGln: 1.357 ± 0.216
2.121TyrArg: 2.121 ± 0.337
1.994TyrSer: 1.994 ± 0.3
1.909TyrThr: 1.909 ± 0.281
2.672TyrVal: 2.672 ± 0.34
0.636TyrTrp: 0.636 ± 0.146
1.4TyrTyr: 1.4 ± 0.275
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 126 proteins (23576 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski