Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus; Brevibacillus brevis

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5887 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C0ZKS4|C0ZKS4_BREBN Uncharacterized protein OS=Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599) OX=358681 GN=yuzB PE=4 SV=1
MM1 pKa = 7.38FGVKK5 pKa = 9.81HH6 pKa = 5.19FNEE9 pKa = 5.15RR10 pKa = 11.84IGACIPVRR18 pKa = 11.84KK19 pKa = 10.05GEE21 pKa = 4.38SNLACPFFQPAVEE34 pKa = 4.11YY35 pKa = 8.32DD36 pKa = 3.35TGGTSPSSITSADD49 pKa = 3.53FNGDD53 pKa = 3.92SIIDD57 pKa = 3.84LAVVDD62 pKa = 4.37IGSNDD67 pKa = 3.3VSILLNNGSGVFSVSQTIPFNTLFPGSLLGQIAAADD103 pKa = 4.07FNGDD107 pKa = 3.15GHH109 pKa = 7.66IDD111 pKa = 3.9LAVTDD116 pKa = 3.81QTPNDD121 pKa = 3.49RR122 pKa = 11.84VFILLNNGDD131 pKa = 3.99GTFAAPVAYY140 pKa = 8.96STGVFPAVSQSFAIATGDD158 pKa = 3.38FDD160 pKa = 4.37GVNGVDD166 pKa = 3.8LAIANVGQITGTSFITVLLNNGSGVFTMAPGSPFATTPGLAPFDD210 pKa = 3.43IVAADD215 pKa = 3.66FNGDD219 pKa = 3.42GKK221 pKa = 11.33LDD223 pKa = 3.72LATANRR229 pKa = 11.84DD230 pKa = 3.66TNDD233 pKa = 2.58ITIFNGNGDD242 pKa = 3.94GTFQTPGVSFSVLPGGASPLSLVAADD268 pKa = 4.28LNGNGSIDD276 pKa = 3.2IAVANIATSNVTVFLNNGFGSFTEE300 pKa = 4.19AAGSPFSLGTDD311 pKa = 3.81AFPFDD316 pKa = 3.22ITAADD321 pKa = 4.53FNCDD325 pKa = 3.15GAIDD329 pKa = 4.39LATPNAGSDD338 pKa = 3.56NVSVLINNGTGGFSTPFQFPTGAGPFSITVADD370 pKa = 4.11FNGDD374 pKa = 3.12GRR376 pKa = 11.84VDD378 pKa = 2.87IATANEE384 pKa = 3.87GSNNVSVLLNGCVQPPTCPPDD405 pKa = 3.42MVVPCPIVNYY415 pKa = 6.66STPVATCPGVTVTCSPPSGSLFPVGDD441 pKa = 3.77TVVTCTAQDD450 pKa = 2.91IFGNTEE456 pKa = 3.61VCSFTITVIDD466 pKa = 4.25EE467 pKa = 4.78PPTITCPEE475 pKa = 5.46DD476 pKa = 2.89ITVPVLPGRR485 pKa = 11.84TSATVTYY492 pKa = 9.74EE493 pKa = 3.68VTATDD498 pKa = 3.58TCGVTSITCHH508 pKa = 4.81GMTRR512 pKa = 11.84SFSPPAPIAVATFTMDD528 pKa = 3.85FPVGMTMLTCTARR541 pKa = 11.84DD542 pKa = 3.49QSGNTASCSFVVTVTFGVPNSIQPEE567 pKa = 4.31IIRR570 pKa = 11.84VKK572 pKa = 10.41KK573 pKa = 9.85VYY575 pKa = 10.44DD576 pKa = 3.49WVVLQSNFRR585 pKa = 11.84LILDD589 pKa = 4.61LNHH592 pKa = 6.72SRR594 pKa = 11.84DD595 pKa = 3.65DD596 pKa = 3.95QGNQKK601 pKa = 10.52

Molecular weight: 61.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C0ZAC3|C0ZAC3_BREBN Uncharacterized protein OS=Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599) OX=358681 GN=BBR47_17550 PE=4 SV=1
MM1 pKa = 7.41KK2 pKa = 10.2PSFNPNNRR10 pKa = 11.84KK11 pKa = 9.37RR12 pKa = 11.84KK13 pKa = 8.62KK14 pKa = 9.81DD15 pKa = 2.98HH16 pKa = 6.23GFRR19 pKa = 11.84KK20 pKa = 10.03RR21 pKa = 11.84MSTKK25 pKa = 10.06NGRR28 pKa = 11.84KK29 pKa = 8.75ILAARR34 pKa = 11.84RR35 pKa = 11.84QRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.84VLSAA44 pKa = 4.11

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5887

0

5887

1813859

27

7745

308.1

34.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.999 ± 0.031

0.765 ± 0.01

4.958 ± 0.024

7.106 ± 0.039

4.132 ± 0.025

7.08 ± 0.032

2.194 ± 0.016

6.647 ± 0.028

5.674 ± 0.033

10.07 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.902 ± 0.017

3.605 ± 0.02

4.089 ± 0.024

4.296 ± 0.03

4.883 ± 0.026

5.968 ± 0.026

5.641 ± 0.028

7.442 ± 0.027

1.224 ± 0.014

3.323 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski