Amino acid dipepetide frequency for Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.997AlaAla: 6.997 ± 0.077	0.728AlaCys: 0.728 ± 0.023	4.023AlaAsp: 4.023 ± 0.045	5.37AlaGlu: 5.37 ± 0.063	3.387AlaPhe: 3.387 ± 0.045	6.167AlaGly: 6.167 ± 0.075	1.581AlaHis: 1.581 ± 0.03	5.805AlaIle: 5.805 ± 0.06	4.843AlaLys: 4.843 ± 0.061	7.93AlaLeu: 7.93 ± 0.068	2.307AlaMet: 2.307 ± 0.039	2.885AlaAsn: 2.885 ± 0.039	2.648AlaPro: 2.648 ± 0.042	2.948AlaGln: 2.948 ± 0.042	3.549AlaArg: 3.549 ± 0.046	4.759AlaSer: 4.759 ± 0.059	4.383AlaThr: 4.383 ± 0.08	6.095AlaVal: 6.095 ± 0.07	0.87AlaTrp: 0.87 ± 0.025	2.71AlaTyr: 2.71 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	0.513CysAla: 0.513 ± 0.018	0.089CysCys: 0.089 ± 0.007	0.388CysAsp: 0.388 ± 0.014	0.475CysGlu: 0.475 ± 0.018	0.341CysPhe: 0.341 ± 0.014	0.682CysGly: 0.682 ± 0.019	0.203CysHis: 0.203 ± 0.011	0.467CysIle: 0.467 ± 0.016	0.365CysLys: 0.365 ± 0.014	0.798CysLeu: 0.798 ± 0.02	0.238CysMet: 0.238 ± 0.013	0.24CysAsn: 0.24 ± 0.012	0.377CysPro: 0.377 ± 0.017	0.3CysGln: 0.3 ± 0.013	0.406CysArg: 0.406 ± 0.015	0.531CysSer: 0.531 ± 0.015	0.4CysThr: 0.4 ± 0.016	0.482CysVal: 0.482 ± 0.017	0.09CysTrp: 0.09 ± 0.007	0.264CysTyr: 0.264 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	3.677AspAla: 3.677 ± 0.048	0.359AspCys: 0.359 ± 0.016	2.405AspAsp: 2.405 ± 0.047	3.981AspGlu: 3.981 ± 0.048	2.141AspPhe: 2.141 ± 0.039	3.553AspGly: 3.553 ± 0.052	1.138AspHis: 1.138 ± 0.027	3.243AspIle: 3.243 ± 0.042	2.813AspLys: 2.813 ± 0.045	4.815AspLeu: 4.815 ± 0.056	1.359AspMet: 1.359 ± 0.03	1.568AspAsn: 1.568 ± 0.029	2.249AspPro: 2.249 ± 0.04	2.153AspGln: 2.153 ± 0.037	2.627AspArg: 2.627 ± 0.039	2.587AspSer: 2.587 ± 0.041	2.452AspThr: 2.452 ± 0.037	3.871AspVal: 3.871 ± 0.051	0.772AspTrp: 0.772 ± 0.024	1.817AspTyr: 1.817 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	6.067GluAla: 6.067 ± 0.065	0.407GluCys: 0.407 ± 0.016	3.145GluAsp: 3.145 ± 0.044	6.074GluGlu: 6.074 ± 0.074	2.085GluPhe: 2.085 ± 0.04	4.313GluGly: 4.313 ± 0.052	1.489GluHis: 1.489 ± 0.035	4.561GluIle: 4.561 ± 0.053	5.246GluLys: 5.246 ± 0.059	7.238GluLeu: 7.238 ± 0.097	2.312GluMet: 2.312 ± 0.039	2.668GluAsn: 2.668 ± 0.038	2.226GluPro: 2.226 ± 0.04	4.114GluGln: 4.114 ± 0.08	4.187GluArg: 4.187 ± 0.047	3.453GluSer: 3.453 ± 0.049	3.49GluThr: 3.49 ± 0.051	4.933GluVal: 4.933 ± 0.052	1.05GluTrp: 1.05 ± 0.026	2.006GluTyr: 2.006 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	3.378PheAla: 3.378 ± 0.046	0.39PheCys: 0.39 ± 0.014	2.204PheAsp: 2.204 ± 0.035	2.404PheGlu: 2.404 ± 0.051	2.024PhePhe: 2.024 ± 0.043	3.098PheGly: 3.098 ± 0.052	1.067PheHis: 1.067 ± 0.027	2.666PheIle: 2.666 ± 0.045	1.487PheLys: 1.487 ± 0.032	4.405PheLeu: 4.405 ± 0.06	1.038PheMet: 1.038 ± 0.026	1.245PheAsn: 1.245 ± 0.03	1.673PhePro: 1.673 ± 0.03	1.58PheGln: 1.58 ± 0.031	1.884PheArg: 1.884 ± 0.036	3.115PheSer: 3.115 ± 0.046	2.6PheThr: 2.6 ± 0.041	3.142PheVal: 3.142 ± 0.047	0.497PheTrp: 0.497 ± 0.017	1.426PheTyr: 1.426 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	5.263GlyAla: 5.263 ± 0.091	0.674GlyCys: 0.674 ± 0.021	3.193GlyAsp: 3.193 ± 0.042	4.68GlyGlu: 4.68 ± 0.057	3.121GlyPhe: 3.121 ± 0.048	4.993GlyGly: 4.993 ± 0.073	1.438GlyHis: 1.438 ± 0.027	5.534GlyIle: 5.534 ± 0.061	4.796GlyLys: 4.796 ± 0.058	6.726GlyLeu: 6.726 ± 0.07	2.373GlyMet: 2.373 ± 0.033	2.582GlyAsn: 2.582 ± 0.044	1.856GlyPro: 1.856 ± 0.032	2.725GlyGln: 2.725 ± 0.052	3.168GlyArg: 3.168 ± 0.047	4.057GlySer: 4.057 ± 0.054	4.299GlyThr: 4.299 ± 0.058	5.533GlyVal: 5.533 ± 0.062	1.026GlyTrp: 1.026 ± 0.028	2.758GlyTyr: 2.758 ± 0.034	0.0GlyXaa: 0.0 ± 0.0
His	1.615HisAla: 1.615 ± 0.03	0.196HisCys: 0.196 ± 0.012	1.101HisAsp: 1.101 ± 0.026	1.411HisGlu: 1.411 ± 0.032	1.098HisPhe: 1.098 ± 0.027	1.482HisGly: 1.482 ± 0.029	0.668HisHis: 0.668 ± 0.025	1.366HisIle: 1.366 ± 0.028	0.908HisLys: 0.908 ± 0.022	2.335HisLeu: 2.335 ± 0.045	0.566HisMet: 0.566 ± 0.017	0.659HisAsn: 0.659 ± 0.019	1.281HisPro: 1.281 ± 0.028	0.955HisGln: 0.955 ± 0.02	1.056HisArg: 1.056 ± 0.025	1.312HisSer: 1.312 ± 0.029	1.153HisThr: 1.153 ± 0.024	1.694HisVal: 1.694 ± 0.033	0.283HisTrp: 0.283 ± 0.013	0.803HisTyr: 0.803 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.027IleAla: 6.027 ± 0.074	0.656IleCys: 0.656 ± 0.019	3.607IleAsp: 3.607 ± 0.049	4.457IleGlu: 4.457 ± 0.058	2.392IlePhe: 2.392 ± 0.043	5.412IleGly: 5.412 ± 0.055	1.531IleHis: 1.531 ± 0.028	3.876IleIle: 3.876 ± 0.056	3.02IleLys: 3.02 ± 0.043	6.144IleLeu: 6.144 ± 0.059	1.65IleMet: 1.65 ± 0.034	2.23IleAsn: 2.23 ± 0.038	3.197IlePro: 3.197 ± 0.042	2.623IleGln: 2.623 ± 0.042	3.498IleArg: 3.498 ± 0.052	4.398IleSer: 4.398 ± 0.057	3.918IleThr: 3.918 ± 0.054	5.201IleVal: 5.201 ± 0.049	0.714IleTrp: 0.714 ± 0.021	1.92IleTyr: 1.92 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	4.458LysAla: 4.458 ± 0.049	0.256LysCys: 0.256 ± 0.013	2.809LysAsp: 2.809 ± 0.044	5.081LysGlu: 5.081 ± 0.065	1.351LysPhe: 1.351 ± 0.029	3.856LysGly: 3.856 ± 0.048	1.149LysHis: 1.149 ± 0.024	3.236LysIle: 3.236 ± 0.056	4.064LysLys: 4.064 ± 0.066	5.275LysLeu: 5.275 ± 0.06	1.884LysMet: 1.884 ± 0.032	2.329LysAsn: 2.329 ± 0.04	2.34LysPro: 2.34 ± 0.043	3.039LysGln: 3.039 ± 0.037	3.184LysArg: 3.184 ± 0.042	2.866LysSer: 2.866 ± 0.042	3.108LysThr: 3.108 ± 0.044	3.983LysVal: 3.983 ± 0.049	0.821LysTrp: 0.821 ± 0.023	1.651LysTyr: 1.651 ± 0.036	0.0LysXaa: 0.0 ± 0.0
Leu	8.896LeuAla: 8.896 ± 0.077	0.824LeuCys: 0.824 ± 0.024	5.037LeuAsp: 5.037 ± 0.058	6.357LeuGlu: 6.357 ± 0.07	4.708LeuPhe: 4.708 ± 0.065	6.856LeuGly: 6.856 ± 0.064	2.364LeuHis: 2.364 ± 0.042	6.248LeuIle: 6.248 ± 0.075	4.775LeuLys: 4.775 ± 0.05	10.915LeuLeu: 10.915 ± 0.105	2.555LeuMet: 2.555 ± 0.037	3.258LeuAsn: 3.258 ± 0.044	4.703LeuPro: 4.703 ± 0.059	4.419LeuGln: 4.419 ± 0.064	4.855LeuArg: 4.855 ± 0.053	6.793LeuSer: 6.793 ± 0.055	5.976LeuThr: 5.976 ± 0.062	7.078LeuVal: 7.078 ± 0.065	0.95LeuTrp: 0.95 ± 0.025	3.133LeuTyr: 3.133 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	2.36MetAla: 2.36 ± 0.04	0.168MetCys: 0.168 ± 0.011	1.562MetAsp: 1.562 ± 0.031	2.154MetGlu: 2.154 ± 0.034	1.029MetPhe: 1.029 ± 0.024	1.908MetGly: 1.908 ± 0.036	0.491MetHis: 0.491 ± 0.017	2.005MetIle: 2.005 ± 0.037	2.054MetLys: 2.054 ± 0.036	2.852MetLeu: 2.852 ± 0.039	0.868MetMet: 0.868 ± 0.022	1.407MetAsn: 1.407 ± 0.03	1.159MetPro: 1.159 ± 0.025	1.145MetGln: 1.145 ± 0.028	1.374MetArg: 1.374 ± 0.031	1.743MetSer: 1.743 ± 0.029	1.75MetThr: 1.75 ± 0.031	1.952MetVal: 1.952 ± 0.036	0.24MetTrp: 0.24 ± 0.011	0.801MetTyr: 0.801 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	2.537AsnAla: 2.537 ± 0.044	0.256AsnCys: 0.256 ± 0.012	1.753AsnAsp: 1.753 ± 0.036	2.502AsnGlu: 2.502 ± 0.036	1.157AsnPhe: 1.157 ± 0.026	3.007AsnGly: 3.007 ± 0.048	0.869AsnHis: 0.869 ± 0.023	2.142AsnIle: 2.142 ± 0.036	2.117AsnLys: 2.117 ± 0.039	3.308AsnLeu: 3.308 ± 0.046	1.002AsnMet: 1.002 ± 0.022	1.369AsnAsn: 1.369 ± 0.032	2.068AsnPro: 2.068 ± 0.036	1.878AsnGln: 1.878 ± 0.041	2.123AsnArg: 2.123 ± 0.036	1.798AsnSer: 1.798 ± 0.035	1.849AsnThr: 1.849 ± 0.038	2.659AsnVal: 2.659 ± 0.043	0.499AsnTrp: 0.499 ± 0.016	1.158AsnTyr: 1.158 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	3.171ProAla: 3.171 ± 0.047	0.222ProCys: 0.222 ± 0.013	2.523ProAsp: 2.523 ± 0.035	3.199ProGlu: 3.199 ± 0.048	2.059ProPhe: 2.059 ± 0.035	2.716ProGly: 2.716 ± 0.053	0.95ProHis: 0.95 ± 0.021	2.812ProIle: 2.812 ± 0.043	1.987ProLys: 1.987 ± 0.035	4.087ProLeu: 4.087 ± 0.063	1.002ProMet: 1.002 ± 0.023	1.522ProAsn: 1.522 ± 0.041	1.374ProPro: 1.374 ± 0.04	1.493ProGln: 1.493 ± 0.028	1.438ProArg: 1.438 ± 0.031	2.515ProSer: 2.515 ± 0.037	2.394ProThr: 2.394 ± 0.04	3.437ProVal: 3.437 ± 0.045	0.448ProTrp: 0.448 ± 0.017	1.537ProTyr: 1.537 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	3.81GlnAla: 3.81 ± 0.057	0.214GlnCys: 0.214 ± 0.011	1.793GlnAsp: 1.793 ± 0.036	3.4GlnGlu: 3.4 ± 0.073	1.597GlnPhe: 1.597 ± 0.034	2.567GlnGly: 2.567 ± 0.041	0.853GlnHis: 0.853 ± 0.025	2.793GlnIle: 2.793 ± 0.036	2.645GlnLys: 2.645 ± 0.044	4.589GlnLeu: 4.589 ± 0.055	1.38GlnMet: 1.38 ± 0.029	1.522GlnAsn: 1.522 ± 0.033	1.731GlnPro: 1.731 ± 0.037	2.249GlnGln: 2.249 ± 0.048	2.047GlnArg: 2.047 ± 0.036	2.37GlnSer: 2.37 ± 0.047	2.376GlnThr: 2.376 ± 0.041	3.258GlnVal: 3.258 ± 0.054	0.513GlnTrp: 0.513 ± 0.017	1.253GlnTyr: 1.253 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	3.276ArgAla: 3.276 ± 0.048	0.351ArgCys: 0.351 ± 0.013	2.445ArgAsp: 2.445 ± 0.038	4.069ArgGlu: 4.069 ± 0.053	2.238ArgPhe: 2.238 ± 0.039	2.762ArgGly: 2.762 ± 0.039	1.053ArgHis: 1.053 ± 0.024	3.468ArgIle: 3.468 ± 0.042	2.994ArgLys: 2.994 ± 0.047	5.215ArgLeu: 5.215 ± 0.056	1.645ArgMet: 1.645 ± 0.032	1.94ArgAsn: 1.94 ± 0.033	1.707ArgPro: 1.707 ± 0.037	2.154ArgGln: 2.154 ± 0.036	2.408ArgArg: 2.408 ± 0.039	2.668ArgSer: 2.668 ± 0.038	2.56ArgThr: 2.56 ± 0.039	3.441ArgVal: 3.441 ± 0.045	0.583ArgTrp: 0.583 ± 0.021	1.852ArgTyr: 1.852 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	4.194SerAla: 4.194 ± 0.048	0.457SerCys: 0.457 ± 0.017	2.692SerAsp: 2.692 ± 0.04	3.615SerGlu: 3.615 ± 0.051	3.113SerPhe: 3.113 ± 0.045	4.504SerGly: 4.504 ± 0.057	1.371SerHis: 1.371 ± 0.033	4.272SerIle: 4.272 ± 0.044	3.115SerLys: 3.115 ± 0.046	6.473SerLeu: 6.473 ± 0.071	1.861SerMet: 1.861 ± 0.036	2.042SerAsn: 2.042 ± 0.033	2.48SerPro: 2.48 ± 0.044	2.243SerGln: 2.243 ± 0.041	2.75SerArg: 2.75 ± 0.037	3.862SerSer: 3.862 ± 0.059	3.273SerThr: 3.273 ± 0.049	4.356SerVal: 4.356 ± 0.053	0.76SerTrp: 0.76 ± 0.022	2.25SerTyr: 2.25 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	4.49ThrAla: 4.49 ± 0.051	0.399ThrCys: 0.399 ± 0.015	2.731ThrAsp: 2.731 ± 0.037	3.544ThrGlu: 3.544 ± 0.043	2.5ThrPhe: 2.5 ± 0.039	4.685ThrGly: 4.685 ± 0.128	1.159ThrHis: 1.159 ± 0.026	4.124ThrIle: 4.124 ± 0.053	2.913ThrLys: 2.913 ± 0.043	5.536ThrLeu: 5.536 ± 0.063	1.527ThrMet: 1.527 ± 0.033	2.116ThrAsn: 2.116 ± 0.034	2.755ThrPro: 2.755 ± 0.045	1.914ThrGln: 1.914 ± 0.038	2.33ThrArg: 2.33 ± 0.039	3.41ThrSer: 3.41 ± 0.043	3.183ThrThr: 3.183 ± 0.057	4.485ThrVal: 4.485 ± 0.058	0.625ThrTrp: 0.625 ± 0.021	1.987ThrTyr: 1.987 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	6.079ValAla: 6.079 ± 0.06	0.617ValCys: 0.617 ± 0.019	3.781ValAsp: 3.781 ± 0.054	5.213ValGlu: 5.213 ± 0.054	2.996ValPhe: 2.996 ± 0.048	5.001ValGly: 5.001 ± 0.055	1.51ValHis: 1.51 ± 0.027	5.154ValIle: 5.154 ± 0.06	4.197ValLys: 4.197 ± 0.055	7.233ValLeu: 7.233 ± 0.061	2.128ValMet: 2.128 ± 0.033	2.805ValAsn: 2.805 ± 0.04	3.162ValPro: 3.162 ± 0.042	2.902ValGln: 2.902 ± 0.049	3.457ValArg: 3.457 ± 0.047	4.772ValSer: 4.772 ± 0.05	4.706ValThr: 4.706 ± 0.07	5.612ValVal: 5.612 ± 0.074	0.843ValTrp: 0.843 ± 0.023	2.254ValTyr: 2.254 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	0.781TrpAla: 0.781 ± 0.02	0.101TrpCys: 0.101 ± 0.008	0.598TrpAsp: 0.598 ± 0.019	0.88TrpGlu: 0.88 ± 0.024	0.527TrpPhe: 0.527 ± 0.017	0.799TrpGly: 0.799 ± 0.022	0.223TrpHis: 0.223 ± 0.012	0.849TrpIle: 0.849 ± 0.024	0.839TrpLys: 0.839 ± 0.024	1.391TrpLeu: 1.391 ± 0.028	0.47TrpMet: 0.47 ± 0.016	0.555TrpAsn: 0.555 ± 0.018	0.344TrpPro: 0.344 ± 0.014	0.544TrpGln: 0.544 ± 0.021	0.598TrpArg: 0.598 ± 0.016	0.746TrpSer: 0.746 ± 0.024	0.628TrpThr: 0.628 ± 0.021	0.792TrpVal: 0.792 ± 0.024	0.184TrpTrp: 0.184 ± 0.009	0.396TrpTyr: 0.396 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.397TyrAla: 2.397 ± 0.034	0.283TyrCys: 0.283 ± 0.014	1.791TyrAsp: 1.791 ± 0.042	2.196TyrGlu: 2.196 ± 0.037	1.461TyrPhe: 1.461 ± 0.032	2.434TyrGly: 2.434 ± 0.041	0.832TyrHis: 0.832 ± 0.025	1.852TyrIle: 1.852 ± 0.038	1.562TyrLys: 1.562 ± 0.033	3.434TyrLeu: 3.434 ± 0.043	0.857TyrMet: 0.857 ± 0.02	1.111TyrAsn: 1.111 ± 0.025	1.556TyrPro: 1.556 ± 0.034	1.583TyrGln: 1.583 ± 0.031	1.889TyrArg: 1.889 ± 0.033	1.929TyrSer: 1.929 ± 0.035	1.917TyrThr: 1.917 ± 0.038	2.419TyrVal: 2.419 ± 0.038	0.474TyrTrp: 0.474 ± 0.016	1.258TyrTyr: 1.258 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5887 proteins (1813859 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---