Methanosaeta harundinacea (strain 6Ac)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanosarcinales; Methanotrichaceae; Methanothrix; Methanosaeta harundinacea

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2358 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G7WKU5|G7WKU5_METH6 UPF0284 protein Mhar_0755 OS=Methanosaeta harundinacea (strain 6Ac) OX=1110509 GN=Mhar_0755 PE=3 SV=1
MM1 pKa = 7.19TMIEE5 pKa = 4.16ITDD8 pKa = 3.5VAAEE12 pKa = 4.04VLKK15 pKa = 11.31GNMIQGRR22 pKa = 11.84VVRR25 pKa = 11.84MILAATDD32 pKa = 3.48ATGANYY38 pKa = 9.67VLAWGDD44 pKa = 3.51PAEE47 pKa = 4.77DD48 pKa = 3.26DD49 pKa = 4.93VVFEE53 pKa = 4.55SNGIKK58 pKa = 10.16VHH60 pKa = 5.86MAPEE64 pKa = 4.29DD65 pKa = 3.78AEE67 pKa = 4.27ILGDD71 pKa = 3.8SPTIIDD77 pKa = 3.89YY78 pKa = 11.41VDD80 pKa = 3.5TEE82 pKa = 4.29DD83 pKa = 4.91LGTGFIIEE91 pKa = 4.18GPEE94 pKa = 4.4AEE96 pKa = 4.55GCGCGHH102 pKa = 7.17DD103 pKa = 4.73HH104 pKa = 7.42DD105 pKa = 6.12HH106 pKa = 8.09DD107 pKa = 5.71DD108 pKa = 4.77GSCGCC113 pKa = 4.74

Molecular weight: 11.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G7WNW5|G7WNW5_METH6 Conserved repeat domain protein OS=Methanosaeta harundinacea (strain 6Ac) OX=1110509 GN=Mhar_1361 PE=4 SV=1
MM1 pKa = 7.52GIIVFALKK9 pKa = 10.7VSLGCGLASLSRR21 pKa = 11.84KK22 pKa = 6.49EE23 pKa = 4.18TLSIGASYY31 pKa = 10.51LALSLVMGLAVGLVPEE47 pKa = 4.32AAITSIMGMGVAMHH61 pKa = 6.71LGISLLLVAVGVSTARR77 pKa = 11.84EE78 pKa = 3.78WNRR81 pKa = 11.84RR82 pKa = 11.84GRR84 pKa = 11.84DD85 pKa = 3.43LSRR88 pKa = 11.84KK89 pKa = 6.27TFWVLSVPCPACLAATFLSCSALAGLLDD117 pKa = 3.69VPGWKK122 pKa = 10.01VGAVVGLIFFISIAILSATFGRR144 pKa = 11.84AGRR147 pKa = 11.84APSALGNAMIFVGLFYY163 pKa = 10.97LLSILLIPAYY173 pKa = 10.03LKK175 pKa = 10.65AASTPFVPIPIPASDD190 pKa = 5.87LIPSYY195 pKa = 11.13LFILGLVGLGFVGRR209 pKa = 11.84RR210 pKa = 11.84MGVFRR215 pKa = 5.67

Molecular weight: 22.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2358

0

2358

730938

37

4560

310.0

34.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.344 ± 0.07

1.094 ± 0.022

5.778 ± 0.044

8.094 ± 0.062

3.599 ± 0.036

9.11 ± 0.059

1.545 ± 0.022

6.066 ± 0.041

4.111 ± 0.053

9.697 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.485 ± 0.029

2.721 ± 0.056

4.834 ± 0.031

2.048 ± 0.023

6.966 ± 0.069

6.171 ± 0.047

4.544 ± 0.054

7.71 ± 0.046

1.191 ± 0.023

2.893 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski