Amino acid dipepetide frequency for Methanosaeta harundinacea (strain 6Ac)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.643AlaAla: 10.643 ± 0.2	0.94AlaCys: 0.94 ± 0.037	5.051AlaAsp: 5.051 ± 0.088	8.236AlaGlu: 8.236 ± 0.151	3.285AlaPhe: 3.285 ± 0.077	8.954AlaGly: 8.954 ± 0.129	1.223AlaHis: 1.223 ± 0.049	5.511AlaIle: 5.511 ± 0.118	3.714AlaLys: 3.714 ± 0.082	9.793AlaLeu: 9.793 ± 0.197	2.204AlaMet: 2.204 ± 0.065	1.984AlaAsn: 1.984 ± 0.069	3.587AlaPro: 3.587 ± 0.087	1.569AlaGln: 1.569 ± 0.05	6.172AlaArg: 6.172 ± 0.113	5.754AlaSer: 5.754 ± 0.106	4.103AlaThr: 4.103 ± 0.088	7.557AlaVal: 7.557 ± 0.122	1.049AlaTrp: 1.049 ± 0.05	2.111AlaTyr: 2.111 ± 0.071	0.0AlaXaa: 0.0 ± 0.0
Cys	0.726CysAla: 0.726 ± 0.032	0.222CysCys: 0.222 ± 0.02	0.711CysAsp: 0.711 ± 0.036	0.7CysGlu: 0.7 ± 0.042	0.335CysPhe: 0.335 ± 0.022	1.438CysGly: 1.438 ± 0.052	0.249CysHis: 0.249 ± 0.019	0.571CysIle: 0.571 ± 0.033	0.378CysLys: 0.378 ± 0.026	0.858CysLeu: 0.858 ± 0.036	0.218CysMet: 0.218 ± 0.019	0.302CysAsn: 0.302 ± 0.02	0.821CysPro: 0.821 ± 0.041	0.238CysGln: 0.238 ± 0.017	0.772CysArg: 0.772 ± 0.033	0.672CysSer: 0.672 ± 0.035	0.599CysThr: 0.599 ± 0.033	0.636CysVal: 0.636 ± 0.032	0.107CysTrp: 0.107 ± 0.01	0.386CysTyr: 0.386 ± 0.03	0.0CysXaa: 0.0 ± 0.0
Asp	4.225AspAla: 4.225 ± 0.08	0.562AspCys: 0.562 ± 0.035	2.926AspAsp: 2.926 ± 0.083	4.513AspGlu: 4.513 ± 0.091	2.238AspPhe: 2.238 ± 0.064	5.704AspGly: 5.704 ± 0.12	1.142AspHis: 1.142 ± 0.042	2.718AspIle: 2.718 ± 0.072	1.762AspLys: 1.762 ± 0.066	8.202AspLeu: 8.202 ± 0.117	1.237AspMet: 1.237 ± 0.041	1.149AspAsn: 1.149 ± 0.062	4.311AspPro: 4.311 ± 0.109	1.421AspGln: 1.421 ± 0.05	4.441AspArg: 4.441 ± 0.096	2.717AspSer: 2.717 ± 0.066	1.688AspThr: 1.688 ± 0.056	4.125AspVal: 4.125 ± 0.077	0.783AspTrp: 0.783 ± 0.039	1.918AspTyr: 1.918 ± 0.062	0.0AspXaa: 0.0 ± 0.0
Glu	9.02GluAla: 9.02 ± 0.17	0.576GluCys: 0.576 ± 0.029	4.342GluAsp: 4.342 ± 0.086	8.53GluGlu: 8.53 ± 0.147	2.495GluPhe: 2.495 ± 0.066	7.339GluGly: 7.339 ± 0.113	0.973GluHis: 0.973 ± 0.037	6.074GluIle: 6.074 ± 0.107	3.716GluLys: 3.716 ± 0.087	6.299GluLeu: 6.299 ± 0.106	2.718GluMet: 2.718 ± 0.06	2.174GluAsn: 2.174 ± 0.09	2.735GluPro: 2.735 ± 0.065	1.062GluGln: 1.062 ± 0.046	6.152GluArg: 6.152 ± 0.14	3.39GluSer: 3.39 ± 0.068	3.426GluThr: 3.426 ± 0.077	7.296GluVal: 7.296 ± 0.116	0.7GluTrp: 0.7 ± 0.033	1.922GluTyr: 1.922 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	3.007PheAla: 3.007 ± 0.065	0.477PheCys: 0.477 ± 0.032	2.556PheAsp: 2.556 ± 0.065	2.772PheGlu: 2.772 ± 0.07	1.379PhePhe: 1.379 ± 0.05	3.582PheGly: 3.582 ± 0.096	0.497PheHis: 0.497 ± 0.026	1.941PheIle: 1.941 ± 0.063	1.331PheLys: 1.331 ± 0.048	3.367PheLeu: 3.367 ± 0.071	0.77PheMet: 0.77 ± 0.036	1.115PheAsn: 1.115 ± 0.055	1.423PhePro: 1.423 ± 0.047	0.835PheGln: 0.835 ± 0.038	2.209PheArg: 2.209 ± 0.054	2.61PheSer: 2.61 ± 0.064	1.673PheThr: 1.673 ± 0.055	2.751PheVal: 2.751 ± 0.059	0.486PheTrp: 0.486 ± 0.032	1.211PheTyr: 1.211 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	7.622GlyAla: 7.622 ± 0.133	1.305GlyCys: 1.305 ± 0.044	5.42GlyAsp: 5.42 ± 0.103	7.373GlyGlu: 7.373 ± 0.121	3.221GlyPhe: 3.221 ± 0.066	8.579GlyGly: 8.579 ± 0.162	1.423GlyHis: 1.423 ± 0.045	5.645GlyIle: 5.645 ± 0.103	3.766GlyLys: 3.766 ± 0.089	7.869GlyLeu: 7.869 ± 0.118	2.604GlyMet: 2.604 ± 0.061	2.277GlyAsn: 2.277 ± 0.088	3.623GlyPro: 3.623 ± 0.081	1.573GlyGln: 1.573 ± 0.05	7.031GlyArg: 7.031 ± 0.118	6.185GlySer: 6.185 ± 0.106	4.398GlyThr: 4.398 ± 0.105	6.831GlyVal: 6.831 ± 0.115	1.404GlyTrp: 1.404 ± 0.065	2.948GlyTyr: 2.948 ± 0.075	0.0GlyXaa: 0.0 ± 0.0
His	1.163HisAla: 1.163 ± 0.044	0.219HisCys: 0.219 ± 0.016	0.822HisAsp: 0.822 ± 0.033	0.761HisGlu: 0.761 ± 0.04	0.636HisPhe: 0.636 ± 0.028	1.151HisGly: 1.151 ± 0.041	0.467HisHis: 0.467 ± 0.03	0.836HisIle: 0.836 ± 0.036	0.46HisLys: 0.46 ± 0.024	2.048HisLeu: 2.048 ± 0.055	0.267HisMet: 0.267 ± 0.019	0.416HisAsn: 0.416 ± 0.025	1.291HisPro: 1.291 ± 0.047	0.617HisGln: 0.617 ± 0.027	1.209HisArg: 1.209 ± 0.04	0.862HisSer: 0.862 ± 0.04	0.617HisThr: 0.617 ± 0.03	0.982HisVal: 0.982 ± 0.037	0.167HisTrp: 0.167 ± 0.015	0.461HisTyr: 0.461 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	5.296IleAla: 5.296 ± 0.093	0.743IleCys: 0.743 ± 0.029	4.069IleAsp: 4.069 ± 0.078	4.652IleGlu: 4.652 ± 0.076	2.635IlePhe: 2.635 ± 0.07	4.872IleGly: 4.872 ± 0.08	0.884IleHis: 0.884 ± 0.037	3.106IleIle: 3.106 ± 0.083	2.474IleLys: 2.474 ± 0.073	6.196IleLeu: 6.196 ± 0.115	1.223IleMet: 1.223 ± 0.047	1.74IleAsn: 1.74 ± 0.055	2.878IlePro: 2.878 ± 0.061	1.335IleGln: 1.335 ± 0.046	3.983IleArg: 3.983 ± 0.083	4.658IleSer: 4.658 ± 0.078	2.873IleThr: 2.873 ± 0.067	4.467IleVal: 4.467 ± 0.09	0.664IleTrp: 0.664 ± 0.033	1.917IleTyr: 1.917 ± 0.06	0.0IleXaa: 0.0 ± 0.0
Lys	3.702LysAla: 3.702 ± 0.077	0.346LysCys: 0.346 ± 0.026	2.353LysAsp: 2.353 ± 0.067	3.396LysGlu: 3.396 ± 0.079	1.264LysPhe: 1.264 ± 0.057	3.371LysGly: 3.371 ± 0.087	0.479LysHis: 0.479 ± 0.028	3.054LysIle: 3.054 ± 0.065	2.294LysLys: 2.294 ± 0.075	3.141LysLeu: 3.141 ± 0.078	1.311LysMet: 1.311 ± 0.048	1.606LysAsn: 1.606 ± 0.049	1.684LysPro: 1.684 ± 0.056	0.658LysGln: 0.658 ± 0.04	2.972LysArg: 2.972 ± 0.076	2.152LysSer: 2.152 ± 0.057	2.242LysThr: 2.242 ± 0.067	3.5LysVal: 3.5 ± 0.083	0.386LysTrp: 0.386 ± 0.024	1.198LysTyr: 1.198 ± 0.046	0.0LysXaa: 0.0 ± 0.0
Leu	10.474LeuAla: 10.474 ± 0.15	0.989LeuCys: 0.989 ± 0.049	5.694LeuAsp: 5.694 ± 0.093	7.752LeuGlu: 7.752 ± 0.124	3.479LeuPhe: 3.479 ± 0.081	8.2LeuGly: 8.2 ± 0.12	1.446LeuHis: 1.446 ± 0.043	5.474LeuIle: 5.474 ± 0.102	4.426LeuLys: 4.426 ± 0.083	9.995LeuLeu: 9.995 ± 0.192	2.207LeuMet: 2.207 ± 0.062	2.536LeuAsn: 2.536 ± 0.068	4.374LeuPro: 4.374 ± 0.079	2.071LeuGln: 2.071 ± 0.057	6.466LeuArg: 6.466 ± 0.118	6.73LeuSer: 6.73 ± 0.099	4.059LeuThr: 4.059 ± 0.085	7.094LeuVal: 7.094 ± 0.113	1.045LeuTrp: 1.045 ± 0.044	2.454LeuTyr: 2.454 ± 0.066	0.0LeuXaa: 0.0 ± 0.0
Met	2.959MetAla: 2.959 ± 0.062	0.109MetCys: 0.109 ± 0.012	1.446MetAsp: 1.446 ± 0.053	2.148MetGlu: 2.148 ± 0.057	0.438MetPhe: 0.438 ± 0.027	2.338MetGly: 2.338 ± 0.06	0.268MetHis: 0.268 ± 0.021	1.772MetIle: 1.772 ± 0.06	1.597MetLys: 1.597 ± 0.048	1.681MetLeu: 1.681 ± 0.054	0.798MetMet: 0.798 ± 0.034	0.841MetAsn: 0.841 ± 0.04	1.078MetPro: 1.078 ± 0.035	0.378MetGln: 0.378 ± 0.026	1.773MetArg: 1.773 ± 0.048	1.089MetSer: 1.089 ± 0.043	1.53MetThr: 1.53 ± 0.053	2.011MetVal: 2.011 ± 0.062	0.123MetTrp: 0.123 ± 0.012	0.472MetTyr: 0.472 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	2.107AsnAla: 2.107 ± 0.069	0.352AsnCys: 0.352 ± 0.026	1.282AsnAsp: 1.282 ± 0.056	1.493AsnGlu: 1.493 ± 0.061	1.222AsnPhe: 1.222 ± 0.059	2.097AsnGly: 2.097 ± 0.069	0.436AsnHis: 0.436 ± 0.024	1.647AsnIle: 1.647 ± 0.063	1.074AsnLys: 1.074 ± 0.056	3.117AsnLeu: 3.117 ± 0.084	0.547AsnMet: 0.547 ± 0.027	1.063AsnAsn: 1.063 ± 0.083	1.788AsnPro: 1.788 ± 0.056	0.666AsnGln: 0.666 ± 0.03	1.835AsnArg: 1.835 ± 0.052	1.586AsnSer: 1.586 ± 0.063	1.136AsnThr: 1.136 ± 0.056	2.396AsnVal: 2.396 ± 0.112	0.395AsnTrp: 0.395 ± 0.028	0.977AsnTyr: 0.977 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	4.326ProAla: 4.326 ± 0.085	0.495ProCys: 0.495 ± 0.027	2.925ProAsp: 2.925 ± 0.079	4.913ProGlu: 4.913 ± 0.087	1.757ProPhe: 1.757 ± 0.046	4.799ProGly: 4.799 ± 0.11	0.815ProHis: 0.815 ± 0.034	2.612ProIle: 2.612 ± 0.059	1.846ProLys: 1.846 ± 0.055	4.438ProLeu: 4.438 ± 0.088	1.06ProMet: 1.06 ± 0.038	1.166ProAsn: 1.166 ± 0.045	2.794ProPro: 2.794 ± 0.086	1.163ProGln: 1.163 ± 0.041	2.773ProArg: 2.773 ± 0.066	3.111ProSer: 3.111 ± 0.082	2.145ProThr: 2.145 ± 0.055	3.396ProVal: 3.396 ± 0.082	0.579ProTrp: 0.579 ± 0.028	1.23ProTyr: 1.23 ± 0.051	0.0ProXaa: 0.0 ± 0.0
Gln	1.854GlnAla: 1.854 ± 0.06	0.171GlnCys: 0.171 ± 0.015	1.181GlnAsp: 1.181 ± 0.048	1.85GlnGlu: 1.85 ± 0.059	0.698GlnPhe: 0.698 ± 0.027	1.606GlnGly: 1.606 ± 0.049	0.317GlnHis: 0.317 ± 0.024	1.536GlnIle: 1.536 ± 0.044	1.055GlnLys: 1.055 ± 0.039	1.714GlnLeu: 1.714 ± 0.051	0.484GlnMet: 0.484 ± 0.029	0.725GlnAsn: 0.725 ± 0.035	0.837GlnPro: 0.837 ± 0.034	0.513GlnGln: 0.513 ± 0.031	1.546GlnArg: 1.546 ± 0.043	1.014GlnSer: 1.014 ± 0.038	0.965GlnThr: 0.965 ± 0.04	1.636GlnVal: 1.636 ± 0.049	0.227GlnTrp: 0.227 ± 0.02	0.55GlnTyr: 0.55 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	6.124ArgAla: 6.124 ± 0.115	0.861ArgCys: 0.861 ± 0.037	3.877ArgAsp: 3.877 ± 0.076	5.623ArgGlu: 5.623 ± 0.114	2.382ArgPhe: 2.382 ± 0.067	6.404ArgGly: 6.404 ± 0.12	1.04ArgHis: 1.04 ± 0.042	4.554ArgIle: 4.554 ± 0.082	2.562ArgLys: 2.562 ± 0.062	6.783ArgLeu: 6.783 ± 0.126	2.126ArgMet: 2.126 ± 0.061	1.612ArgAsn: 1.612 ± 0.052	3.195ArgPro: 3.195 ± 0.075	1.311ArgGln: 1.311 ± 0.046	5.913ArgArg: 5.913 ± 0.132	4.423ArgSer: 4.423 ± 0.091	2.861ArgThr: 2.861 ± 0.072	4.881ArgVal: 4.881 ± 0.09	0.933ArgTrp: 0.933 ± 0.038	2.197ArgTyr: 2.197 ± 0.061	0.0ArgXaa: 0.0 ± 0.0
Ser	5.392SerAla: 5.392 ± 0.092	0.72SerCys: 0.72 ± 0.033	3.374SerAsp: 3.374 ± 0.067	4.36SerGlu: 4.36 ± 0.079	2.542SerPhe: 2.542 ± 0.075	6.463SerGly: 6.463 ± 0.116	0.921SerHis: 0.921 ± 0.033	3.838SerIle: 3.838 ± 0.08	2.506SerLys: 2.506 ± 0.062	5.723SerLeu: 5.723 ± 0.11	1.465SerMet: 1.465 ± 0.042	1.587SerAsn: 1.587 ± 0.055	3.513SerPro: 3.513 ± 0.075	1.387SerGln: 1.387 ± 0.048	4.018SerArg: 4.018 ± 0.092	4.33SerSer: 4.33 ± 0.106	2.764SerThr: 2.764 ± 0.068	4.004SerVal: 4.004 ± 0.082	0.893SerTrp: 0.893 ± 0.044	1.906SerTyr: 1.906 ± 0.056	0.0SerXaa: 0.0 ± 0.0
Thr	4.136ThrAla: 4.136 ± 0.097	0.527ThrCys: 0.527 ± 0.034	2.442ThrAsp: 2.442 ± 0.069	2.833ThrGlu: 2.833 ± 0.07	1.799ThrPhe: 1.799 ± 0.057	4.519ThrGly: 4.519 ± 0.092	0.739ThrHis: 0.739 ± 0.036	3.106ThrIle: 3.106 ± 0.084	1.72ThrLys: 1.72 ± 0.057	4.489ThrLeu: 4.489 ± 0.099	0.895ThrMet: 0.895 ± 0.038	1.419ThrAsn: 1.419 ± 0.071	2.617ThrPro: 2.617 ± 0.072	0.691ThrGln: 0.691 ± 0.034	2.5ThrArg: 2.5 ± 0.059	2.859ThrSer: 2.859 ± 0.08	2.457ThrThr: 2.457 ± 0.073	3.779ThrVal: 3.779 ± 0.092	0.535ThrTrp: 0.535 ± 0.036	1.376ThrTyr: 1.376 ± 0.05	0.0ThrXaa: 0.0 ± 0.0
Val	7.525ValAla: 7.525 ± 0.118	0.852ValCys: 0.852 ± 0.038	4.621ValAsp: 4.621 ± 0.079	6.508ValGlu: 6.508 ± 0.101	2.606ValPhe: 2.606 ± 0.062	6.395ValGly: 6.395 ± 0.11	1.304ValHis: 1.304 ± 0.044	4.352ValIle: 4.352 ± 0.086	2.914ValLys: 2.914 ± 0.073	7.287ValLeu: 7.287 ± 0.105	1.831ValMet: 1.831 ± 0.049	1.937ValAsn: 1.937 ± 0.073	3.698ValPro: 3.698 ± 0.081	1.735ValGln: 1.735 ± 0.05	4.907ValArg: 4.907 ± 0.099	4.658ValSer: 4.658 ± 0.078	3.765ValThr: 3.765 ± 0.143	7.079ValVal: 7.079 ± 0.134	0.867ValTrp: 0.867 ± 0.054	2.259ValTyr: 2.259 ± 0.059	0.0ValXaa: 0.0 ± 0.0
Trp	0.876TrpAla: 0.876 ± 0.039	0.129TrpCys: 0.129 ± 0.016	0.757TrpAsp: 0.757 ± 0.045	0.772TrpGlu: 0.772 ± 0.032	0.401TrpPhe: 0.401 ± 0.027	0.897TrpGly: 0.897 ± 0.044	0.201TrpHis: 0.201 ± 0.02	0.787TrpIle: 0.787 ± 0.032	0.521TrpLys: 0.521 ± 0.031	1.012TrpLeu: 1.012 ± 0.044	0.345TrpMet: 0.345 ± 0.026	0.514TrpAsn: 0.514 ± 0.034	0.471TrpPro: 0.471 ± 0.03	0.371TrpGln: 0.371 ± 0.023	0.915TrpArg: 0.915 ± 0.039	0.832TrpSer: 0.832 ± 0.04	0.776TrpThr: 0.776 ± 0.043	0.688TrpVal: 0.688 ± 0.032	0.216TrpTrp: 0.216 ± 0.019	0.427TrpTyr: 0.427 ± 0.033	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.266TyrAla: 2.266 ± 0.06	0.343TyrCys: 0.343 ± 0.024	1.933TyrAsp: 1.933 ± 0.089	1.757TyrGlu: 1.757 ± 0.056	1.181TyrPhe: 1.181 ± 0.048	2.389TyrGly: 2.389 ± 0.058	0.629TyrHis: 0.629 ± 0.031	1.531TyrIle: 1.531 ± 0.05	0.993TyrLys: 0.993 ± 0.042	2.954TyrLeu: 2.954 ± 0.068	0.539TyrMet: 0.539 ± 0.029	1.051TyrAsn: 1.051 ± 0.046	1.624TyrPro: 1.624 ± 0.049	0.885TyrGln: 0.885 ± 0.039	2.075TyrArg: 2.075 ± 0.056	2.073TyrSer: 2.073 ± 0.058	1.36TyrThr: 1.36 ± 0.054	1.991TyrVal: 1.991 ± 0.056	0.347TyrTrp: 0.347 ± 0.025	1.006TyrTyr: 1.006 ± 0.042	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2358 proteins (730938 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---