Bacillus vireti LMG 21834

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Neobacillus; Neobacillus vireti

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5084 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W1SH44|W1SH44_9BACI ATP-dependent 6-phosphofructokinase OS=Bacillus vireti LMG 21834 OX=1131730 GN=pfkA PE=3 SV=1
MM1 pKa = 7.45DD2 pKa = 5.13HH3 pKa = 6.47KK4 pKa = 10.5HH5 pKa = 4.96EE6 pKa = 5.01HH7 pKa = 5.63EE8 pKa = 4.75HH9 pKa = 8.23DD10 pKa = 3.44EE11 pKa = 4.26DD12 pKa = 5.34RR13 pKa = 11.84YY14 pKa = 9.58ITLIDD19 pKa = 3.8EE20 pKa = 4.92NGDD23 pKa = 3.48EE24 pKa = 4.21QLFEE28 pKa = 4.11ILFTFDD34 pKa = 2.77SDD36 pKa = 3.82EE37 pKa = 4.28FEE39 pKa = 3.87KK40 pKa = 10.9SYY42 pKa = 11.47VFFYY46 pKa = 10.58PVGASEE52 pKa = 4.38EE53 pKa = 4.22EE54 pKa = 4.36EE55 pKa = 4.09IDD57 pKa = 3.33ILTYY61 pKa = 10.6AYY63 pKa = 9.75IPTDD67 pKa = 3.53DD68 pKa = 4.15GGFGEE73 pKa = 4.67LMEE76 pKa = 5.73IEE78 pKa = 4.19TDD80 pKa = 3.83EE81 pKa = 4.05EE82 pKa = 4.52WEE84 pKa = 4.05MVEE87 pKa = 5.13EE88 pKa = 4.24VFNTFNEE95 pKa = 4.35EE96 pKa = 3.95QEE98 pKa = 4.29EE99 pKa = 4.34EE100 pKa = 4.16

Molecular weight: 12.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W1SAT4|W1SAT4_9BACI Spore coat protein F putative OS=Bacillus vireti LMG 21834 OX=1131730 GN=BAVI_23478 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.38RR3 pKa = 11.84TYY5 pKa = 10.17QPNSRR10 pKa = 11.84KK11 pKa = 9.39HH12 pKa = 5.91SKK14 pKa = 9.08VHH16 pKa = 5.89GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.7VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5084

0

5084

1483976

20

2103

291.9

32.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.028 ± 0.034

0.748 ± 0.011

4.908 ± 0.028

7.156 ± 0.047

4.76 ± 0.027

6.951 ± 0.033

2.028 ± 0.017

8.041 ± 0.037

7.247 ± 0.033

9.878 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.721 ± 0.015

4.615 ± 0.03

3.627 ± 0.019

3.654 ± 0.022

3.92 ± 0.025

5.954 ± 0.025

5.347 ± 0.021

6.858 ± 0.03

1.073 ± 0.015

3.486 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski