Amino acid dipepetide frequency for Bacillus vireti LMG 21834

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.413AlaAla: 6.413 ± 0.075
0.594AlaCys: 0.594 ± 0.021
3.468AlaAsp: 3.468 ± 0.05
4.785AlaGlu: 4.785 ± 0.067
3.21AlaPhe: 3.21 ± 0.053
5.477AlaGly: 5.477 ± 0.078
1.272AlaHis: 1.272 ± 0.031
5.945AlaIle: 5.945 ± 0.071
4.979AlaLys: 4.979 ± 0.066
6.956AlaLeu: 6.956 ± 0.075
1.986AlaMet: 1.986 ± 0.038
3.027AlaAsn: 3.027 ± 0.055
2.138AlaPro: 2.138 ± 0.042
2.109AlaGln: 2.109 ± 0.041
2.544AlaArg: 2.544 ± 0.046
3.907AlaSer: 3.907 ± 0.058
3.242AlaThr: 3.242 ± 0.045
5.399AlaVal: 5.399 ± 0.064
0.637AlaTrp: 0.637 ± 0.024
2.191AlaTyr: 2.191 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
0.454CysAla: 0.454 ± 0.019
0.111CysCys: 0.111 ± 0.009
0.385CysAsp: 0.385 ± 0.016
0.494CysGlu: 0.494 ± 0.018
0.371CysPhe: 0.371 ± 0.016
0.687CysGly: 0.687 ± 0.025
0.25CysHis: 0.25 ± 0.016
0.573CysIle: 0.573 ± 0.018
0.388CysLys: 0.388 ± 0.017
0.726CysLeu: 0.726 ± 0.023
0.175CysMet: 0.175 ± 0.011
0.283CysAsn: 0.283 ± 0.015
0.375CysPro: 0.375 ± 0.02
0.235CysGln: 0.235 ± 0.013
0.29CysArg: 0.29 ± 0.013
0.532CysSer: 0.532 ± 0.018
0.381CysThr: 0.381 ± 0.016
0.434CysVal: 0.434 ± 0.018
0.082CysTrp: 0.082 ± 0.008
0.259CysTyr: 0.259 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
3.107AspAla: 3.107 ± 0.051
0.379AspCys: 0.379 ± 0.015
2.345AspAsp: 2.345 ± 0.048
3.893AspGlu: 3.893 ± 0.06
2.52AspPhe: 2.52 ± 0.038
3.403AspGly: 3.403 ± 0.061
1.079AspHis: 1.079 ± 0.029
3.915AspIle: 3.915 ± 0.05
3.212AspLys: 3.212 ± 0.053
4.908AspLeu: 4.908 ± 0.062
1.229AspMet: 1.229 ± 0.033
1.812AspAsn: 1.812 ± 0.038
1.876AspPro: 1.876 ± 0.04
1.906AspGln: 1.906 ± 0.04
2.171AspArg: 2.171 ± 0.039
2.667AspSer: 2.667 ± 0.049
2.296AspThr: 2.296 ± 0.043
3.605AspVal: 3.605 ± 0.055
0.656AspTrp: 0.656 ± 0.025
2.102AspTyr: 2.102 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
5.033GluAla: 5.033 ± 0.071
0.378GluCys: 0.378 ± 0.016
3.301GluAsp: 3.301 ± 0.052
6.194GluGlu: 6.194 ± 0.086
2.711GluPhe: 2.711 ± 0.047
3.958GluGly: 3.958 ± 0.058
1.41GluHis: 1.41 ± 0.032
5.834GluIle: 5.834 ± 0.074
6.746GluLys: 6.746 ± 0.083
7.0GluLeu: 7.0 ± 0.089
2.266GluMet: 2.266 ± 0.037
3.766GluAsn: 3.766 ± 0.051
1.859GluPro: 1.859 ± 0.038
3.104GluGln: 3.104 ± 0.054
3.207GluArg: 3.207 ± 0.052
3.255GluSer: 3.255 ± 0.058
3.747GluThr: 3.747 ± 0.051
4.753GluVal: 4.753 ± 0.062
0.792GluTrp: 0.792 ± 0.023
2.248GluTyr: 2.248 ± 0.045
0.0GluXaa: 0.0 ± 0.0
Phe
3.253PheAla: 3.253 ± 0.051
0.401PheCys: 0.401 ± 0.016
2.274PheAsp: 2.274 ± 0.037
2.945PheGlu: 2.945 ± 0.048
2.59PhePhe: 2.59 ± 0.051
3.41PheGly: 3.41 ± 0.056
1.069PheHis: 1.069 ± 0.026
4.067PheIle: 4.067 ± 0.062
2.668PheLys: 2.668 ± 0.041
4.979PheLeu: 4.979 ± 0.069
1.224PheMet: 1.224 ± 0.033
1.987PheAsn: 1.987 ± 0.04
1.754PhePro: 1.754 ± 0.035
1.676PheGln: 1.676 ± 0.038
1.669PheArg: 1.669 ± 0.037
3.448PheSer: 3.448 ± 0.044
2.603PheThr: 2.603 ± 0.046
3.257PheVal: 3.257 ± 0.05
0.522PheTrp: 0.522 ± 0.021
1.801PheTyr: 1.801 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
4.933GlyAla: 4.933 ± 0.064
0.63GlyCys: 0.63 ± 0.023
3.057GlyAsp: 3.057 ± 0.052
4.305GlyGlu: 4.305 ± 0.062
3.598GlyPhe: 3.598 ± 0.052
4.917GlyGly: 4.917 ± 0.083
1.388GlyHis: 1.388 ± 0.032
6.039GlyIle: 6.039 ± 0.077
5.468GlyLys: 5.468 ± 0.072
6.674GlyLeu: 6.674 ± 0.058
2.173GlyMet: 2.173 ± 0.043
2.947GlyAsn: 2.947 ± 0.054
1.793GlyPro: 1.793 ± 0.04
2.196GlyGln: 2.196 ± 0.045
2.612GlyArg: 2.612 ± 0.043
3.976GlySer: 3.976 ± 0.063
4.121GlyThr: 4.121 ± 0.057
4.933GlyVal: 4.933 ± 0.064
0.945GlyTrp: 0.945 ± 0.038
2.805GlyTyr: 2.805 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
1.237HisAla: 1.237 ± 0.032
0.186HisCys: 0.186 ± 0.011
1.069HisAsp: 1.069 ± 0.03
1.313HisGlu: 1.313 ± 0.031
1.09HisPhe: 1.09 ± 0.029
1.406HisGly: 1.406 ± 0.034
0.645HisHis: 0.645 ± 0.022
1.412HisIle: 1.412 ± 0.032
1.038HisLys: 1.038 ± 0.028
2.096HisLeu: 2.096 ± 0.043
0.455HisMet: 0.455 ± 0.017
0.801HisAsn: 0.801 ± 0.028
1.113HisPro: 1.113 ± 0.029
0.836HisGln: 0.836 ± 0.024
0.819HisArg: 0.819 ± 0.028
1.273HisSer: 1.273 ± 0.03
1.016HisThr: 1.016 ± 0.027
1.35HisVal: 1.35 ± 0.029
0.249HisTrp: 0.249 ± 0.013
0.877HisTyr: 0.877 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
6.001IleAla: 6.001 ± 0.076
0.674IleCys: 0.674 ± 0.021
4.223IleAsp: 4.223 ± 0.056
5.611IleGlu: 5.611 ± 0.077
3.634IlePhe: 3.634 ± 0.054
6.096IleGly: 6.096 ± 0.073
1.744IleHis: 1.744 ± 0.039
6.363IleIle: 6.363 ± 0.083
5.055IleLys: 5.055 ± 0.061
7.572IleLeu: 7.572 ± 0.08
1.834IleMet: 1.834 ± 0.036
3.657IleAsn: 3.657 ± 0.053
3.542IlePro: 3.542 ± 0.054
2.899IleGln: 2.899 ± 0.047
3.074IleArg: 3.074 ± 0.049
5.305IleSer: 5.305 ± 0.062
4.505IleThr: 4.505 ± 0.053
5.478IleVal: 5.478 ± 0.075
0.676IleTrp: 0.676 ± 0.023
2.47IleTyr: 2.47 ± 0.036
0.0IleXaa: 0.0 ± 0.0
Lys
4.939LysAla: 4.939 ± 0.07
0.354LysCys: 0.354 ± 0.016
3.991LysAsp: 3.991 ± 0.069
6.611LysGlu: 6.611 ± 0.082
2.18LysPhe: 2.18 ± 0.045
4.801LysGly: 4.801 ± 0.061
1.238LysHis: 1.238 ± 0.029
5.208LysIle: 5.208 ± 0.069
6.194LysLys: 6.194 ± 0.071
6.348LysLeu: 6.348 ± 0.065
2.255LysMet: 2.255 ± 0.041
3.776LysAsn: 3.776 ± 0.054
2.359LysPro: 2.359 ± 0.043
3.187LysGln: 3.187 ± 0.054
3.288LysArg: 3.288 ± 0.045
3.704LysSer: 3.704 ± 0.053
3.946LysThr: 3.946 ± 0.056
4.83LysVal: 4.83 ± 0.057
0.97LysTrp: 0.97 ± 0.033
2.296LysTyr: 2.296 ± 0.046
0.0LysXaa: 0.0 ± 0.0
Leu
7.41LeuAla: 7.41 ± 0.074
0.724LeuCys: 0.724 ± 0.022
4.758LeuAsp: 4.758 ± 0.067
6.558LeuGlu: 6.558 ± 0.079
5.148LeuPhe: 5.148 ± 0.077
6.522LeuGly: 6.522 ± 0.074
1.881LeuHis: 1.881 ± 0.04
7.562LeuIle: 7.562 ± 0.089
7.107LeuLys: 7.107 ± 0.08
10.469LeuLeu: 10.469 ± 0.109
2.489LeuMet: 2.489 ± 0.047
4.456LeuAsn: 4.456 ± 0.055
3.897LeuPro: 3.897 ± 0.059
3.5LeuGln: 3.5 ± 0.059
3.564LeuArg: 3.564 ± 0.054
6.702LeuSer: 6.702 ± 0.065
5.733LeuThr: 5.733 ± 0.066
6.395LeuVal: 6.395 ± 0.062
0.833LeuTrp: 0.833 ± 0.026
3.068LeuTyr: 3.068 ± 0.048
0.0LeuXaa: 0.0 ± 0.0
Met
2.036MetAla: 2.036 ± 0.037
0.16MetCys: 0.16 ± 0.01
1.423MetAsp: 1.423 ± 0.033
1.921MetGlu: 1.921 ± 0.036
1.068MetPhe: 1.068 ± 0.025
1.8MetGly: 1.8 ± 0.036
0.38MetHis: 0.38 ± 0.015
2.214MetIle: 2.214 ± 0.036
2.5MetLys: 2.5 ± 0.038
2.511MetLeu: 2.511 ± 0.042
0.848MetMet: 0.848 ± 0.022
1.602MetAsn: 1.602 ± 0.034
0.997MetPro: 0.997 ± 0.029
0.863MetGln: 0.863 ± 0.024
1.014MetArg: 1.014 ± 0.029
1.556MetSer: 1.556 ± 0.032
1.544MetThr: 1.544 ± 0.028
1.886MetVal: 1.886 ± 0.036
0.173MetTrp: 0.173 ± 0.011
0.709MetTyr: 0.709 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
2.739AsnAla: 2.739 ± 0.047
0.341AsnCys: 0.341 ± 0.014
2.244AsnAsp: 2.244 ± 0.048
3.492AsnGlu: 3.492 ± 0.048
1.845AsnPhe: 1.845 ± 0.04
3.631AsnGly: 3.631 ± 0.055
1.135AsnHis: 1.135 ± 0.031
3.458AsnIle: 3.458 ± 0.042
3.281AsnLys: 3.281 ± 0.056
4.214AsnLeu: 4.214 ± 0.049
1.17AsnMet: 1.17 ± 0.029
2.188AsnAsn: 2.188 ± 0.046
2.299AsnPro: 2.299 ± 0.041
2.113AsnGln: 2.113 ± 0.04
2.115AsnArg: 2.115 ± 0.033
2.56AsnSer: 2.56 ± 0.037
2.193AsnThr: 2.193 ± 0.042
2.935AsnVal: 2.935 ± 0.046
0.619AsnTrp: 0.619 ± 0.023
1.575AsnTyr: 1.575 ± 0.038
0.0AsnXaa: 0.0 ± 0.0
Pro
2.466ProAla: 2.466 ± 0.045
0.233ProCys: 0.233 ± 0.014
1.954ProAsp: 1.954 ± 0.045
2.896ProGlu: 2.896 ± 0.047
2.074ProPhe: 2.074 ± 0.036
2.352ProGly: 2.352 ± 0.047
0.749ProHis: 0.749 ± 0.025
2.998ProIle: 2.998 ± 0.048
2.401ProLys: 2.401 ± 0.043
3.521ProLeu: 3.521 ± 0.053
0.836ProMet: 0.836 ± 0.026
1.791ProAsn: 1.791 ± 0.033
1.013ProPro: 1.013 ± 0.034
1.144ProGln: 1.144 ± 0.028
1.116ProArg: 1.116 ± 0.029
2.229ProSer: 2.229 ± 0.045
1.883ProThr: 1.883 ± 0.035
2.815ProVal: 2.815 ± 0.045
0.379ProTrp: 0.379 ± 0.018
1.423ProTyr: 1.423 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
2.666GlnAla: 2.666 ± 0.05
0.21GlnCys: 0.21 ± 0.014
1.52GlnAsp: 1.52 ± 0.031
2.718GlnGlu: 2.718 ± 0.042
1.704GlnPhe: 1.704 ± 0.036
2.138GlnGly: 2.138 ± 0.036
0.722GlnHis: 0.722 ± 0.025
2.726GlnIle: 2.726 ± 0.044
2.78GlnLys: 2.78 ± 0.049
3.987GlnLeu: 3.987 ± 0.065
1.121GlnMet: 1.121 ± 0.028
1.726GlnAsn: 1.726 ± 0.035
1.21GlnPro: 1.21 ± 0.031
1.751GlnGln: 1.751 ± 0.043
1.402GlnArg: 1.402 ± 0.031
2.051GlnSer: 2.051 ± 0.039
2.057GlnThr: 2.057 ± 0.036
2.299GlnVal: 2.299 ± 0.044
0.412GlnTrp: 0.412 ± 0.018
1.339GlnTyr: 1.339 ± 0.029
0.0GlnXaa: 0.0 ± 0.0
Arg
2.309ArgAla: 2.309 ± 0.044
0.257ArgCys: 0.257 ± 0.015
1.948ArgAsp: 1.948 ± 0.038
3.062ArgGlu: 3.062 ± 0.053
1.974ArgPhe: 1.974 ± 0.038
2.313ArgGly: 2.313 ± 0.037
0.776ArgHis: 0.776 ± 0.023
3.16ArgIle: 3.16 ± 0.049
3.23ArgLys: 3.23 ± 0.05
3.964ArgLeu: 3.964 ± 0.06
1.251ArgMet: 1.251 ± 0.03
1.945ArgAsn: 1.945 ± 0.035
1.323ArgPro: 1.323 ± 0.034
1.394ArgGln: 1.394 ± 0.031
1.758ArgArg: 1.758 ± 0.041
2.136ArgSer: 2.136 ± 0.04
2.011ArgThr: 2.011 ± 0.039
2.543ArgVal: 2.543 ± 0.044
0.373ArgTrp: 0.373 ± 0.019
1.468ArgTyr: 1.468 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
3.774SerAla: 3.774 ± 0.053
0.466SerCys: 0.466 ± 0.019
2.654SerAsp: 2.654 ± 0.053
3.831SerGlu: 3.831 ± 0.053
3.514SerPhe: 3.514 ± 0.051
4.631SerGly: 4.631 ± 0.062
1.197SerHis: 1.197 ± 0.027
5.045SerIle: 5.045 ± 0.07
3.953SerLys: 3.953 ± 0.053
6.111SerLeu: 6.111 ± 0.071
1.65SerMet: 1.65 ± 0.033
2.58SerAsn: 2.58 ± 0.047
2.171SerPro: 2.171 ± 0.044
1.967SerGln: 1.967 ± 0.037
2.266SerArg: 2.266 ± 0.041
3.861SerSer: 3.861 ± 0.062
3.097SerThr: 3.097 ± 0.047
3.921SerVal: 3.921 ± 0.057
0.644SerTrp: 0.644 ± 0.022
2.21SerTyr: 2.21 ± 0.037
0.0SerXaa: 0.0 ± 0.0
Thr
4.167ThrAla: 4.167 ± 0.05
0.371ThrCys: 0.371 ± 0.017
2.584ThrAsp: 2.584 ± 0.052
3.343ThrGlu: 3.343 ± 0.049
2.579ThrPhe: 2.579 ± 0.044
4.299ThrGly: 4.299 ± 0.073
1.013ThrHis: 1.013 ± 0.025
4.753ThrIle: 4.753 ± 0.057
3.61ThrLys: 3.61 ± 0.049
5.013ThrLeu: 5.013 ± 0.056
1.289ThrMet: 1.289 ± 0.027
2.542ThrAsn: 2.542 ± 0.04
2.202ThrPro: 2.202 ± 0.045
1.343ThrGln: 1.343 ± 0.031
1.78ThrArg: 1.78 ± 0.034
3.173ThrSer: 3.173 ± 0.05
2.87ThrThr: 2.87 ± 0.051
4.144ThrVal: 4.144 ± 0.062
0.56ThrTrp: 0.56 ± 0.022
1.832ThrTyr: 1.832 ± 0.037
0.0ThrXaa: 0.0 ± 0.0
Val
4.71ValAla: 4.71 ± 0.076
0.594ValCys: 0.594 ± 0.019
3.532ValAsp: 3.532 ± 0.058
4.667ValGlu: 4.667 ± 0.061
3.292ValPhe: 3.292 ± 0.048
4.472ValGly: 4.472 ± 0.06
1.296ValHis: 1.296 ± 0.032
5.764ValIle: 5.764 ± 0.058
4.94ValLys: 4.94 ± 0.063
6.716ValLeu: 6.716 ± 0.071
1.797ValMet: 1.797 ± 0.034
3.249ValAsn: 3.249 ± 0.045
2.702ValPro: 2.702 ± 0.041
2.233ValGln: 2.233 ± 0.041
2.481ValArg: 2.481 ± 0.043
4.454ValSer: 4.454 ± 0.055
4.043ValThr: 4.043 ± 0.064
4.777ValVal: 4.777 ± 0.07
0.66ValTrp: 0.66 ± 0.024
2.202ValTyr: 2.202 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
0.673TrpAla: 0.673 ± 0.023
0.087TrpCys: 0.087 ± 0.008
0.557TrpAsp: 0.557 ± 0.021
0.663TrpGlu: 0.663 ± 0.019
0.55TrpPhe: 0.55 ± 0.019
0.711TrpGly: 0.711 ± 0.025
0.201TrpHis: 0.201 ± 0.011
0.914TrpIle: 0.914 ± 0.026
0.791TrpLys: 0.791 ± 0.024
1.223TrpLeu: 1.223 ± 0.037
0.325TrpMet: 0.325 ± 0.015
0.569TrpAsn: 0.569 ± 0.018
0.241TrpPro: 0.241 ± 0.013
0.376TrpGln: 0.376 ± 0.017
0.433TrpArg: 0.433 ± 0.018
0.593TrpSer: 0.593 ± 0.024
0.497TrpThr: 0.497 ± 0.019
0.718TrpVal: 0.718 ± 0.022
0.148TrpTrp: 0.148 ± 0.009
0.464TrpTyr: 0.464 ± 0.029
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.96TyrAla: 1.96 ± 0.035
0.336TyrCys: 0.336 ± 0.017
1.793TyrAsp: 1.793 ± 0.036
2.262TyrGlu: 2.262 ± 0.042
1.944TyrPhe: 1.944 ± 0.039
2.485TyrGly: 2.485 ± 0.039
0.838TyrHis: 0.838 ± 0.025
2.462TyrIle: 2.462 ± 0.044
2.133TyrLys: 2.133 ± 0.039
3.788TyrLeu: 3.788 ± 0.053
0.832TyrMet: 0.832 ± 0.025
1.441TyrAsn: 1.441 ± 0.031
1.409TyrPro: 1.409 ± 0.035
1.707TyrGln: 1.707 ± 0.038
1.592TyrArg: 1.592 ± 0.036
2.159TyrSer: 2.159 ± 0.044
1.683TyrThr: 1.683 ± 0.029
2.108TyrVal: 2.108 ± 0.044
0.406TyrTrp: 0.406 ± 0.018
1.52TyrTyr: 1.52 ± 0.037
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5084 proteins (1483976 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski