Legionella spiritensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2908 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0W0ZAZ1|A0A0W0ZAZ1_LEGSP Uncharacterized protein OS=Legionella spiritensis OX=452 GN=Lspi_0051 PE=4 SV=1
MM1 pKa = 7.47VGVFMVVPSLSGVVQGLEE19 pKa = 4.29GILIKK24 pKa = 9.62NTRR27 pKa = 11.84DD28 pKa = 3.24GGNEE32 pKa = 3.82VAKK35 pKa = 10.7LGTPVHH41 pKa = 6.18QGDD44 pKa = 4.2ILTLLSGNAYY54 pKa = 9.2IHH56 pKa = 5.83MLEE59 pKa = 4.55GFPEE63 pKa = 4.18ALALNRR69 pKa = 11.84PVQIDD74 pKa = 4.75GISPLLKK81 pKa = 10.26AAPQEE86 pKa = 4.31SLVEE90 pKa = 4.1QIVDD94 pKa = 3.26EE95 pKa = 4.59ALAKK99 pKa = 10.91GIDD102 pKa = 3.4PTIILNVLEE111 pKa = 4.03EE112 pKa = 4.15TAAGEE117 pKa = 4.15EE118 pKa = 4.56TIGEE122 pKa = 4.15GGRR125 pKa = 11.84FFVLDD130 pKa = 3.64PLYY133 pKa = 11.09GVGAVTAGYY142 pKa = 6.63PTVGVSVDD150 pKa = 3.7TNSGEE155 pKa = 3.92QHH157 pKa = 5.28VFFYY161 pKa = 10.88EE162 pKa = 4.18PDD164 pKa = 3.52SSEE167 pKa = 3.93QASDD171 pKa = 3.79FVSNPDD177 pKa = 3.32VTEE180 pKa = 4.07TTPFAIGVSSVSVDD194 pKa = 3.42EE195 pKa = 4.49SDD197 pKa = 4.24GLATVVNRR205 pKa = 11.84VQSSTAASEE214 pKa = 4.06ITSVEE219 pKa = 3.98LSTPDD224 pKa = 2.88SNAAVWHH231 pKa = 6.68SGTQTLTATDD241 pKa = 4.78GSWQIMVVRR250 pKa = 11.84SGNNEE255 pKa = 3.08INYY258 pKa = 8.62EE259 pKa = 4.38FKK261 pKa = 10.77QLTPLQHH268 pKa = 7.25PDD270 pKa = 3.05ASDD273 pKa = 3.79PNDD276 pKa = 4.86AITVPITVTATRR288 pKa = 11.84NDD290 pKa = 3.71GASATDD296 pKa = 3.42RR297 pKa = 11.84FSVTIYY303 pKa = 11.04DD304 pKa = 4.43DD305 pKa = 3.97GPKK308 pKa = 9.48IEE310 pKa = 4.26EE311 pKa = 4.39ALVEE315 pKa = 4.63SPLPLNVDD323 pKa = 3.42EE324 pKa = 5.0TNISSGNAVATVNYY338 pKa = 9.54FNTFAGAVHH347 pKa = 6.98FGMDD351 pKa = 4.05DD352 pKa = 3.64TLDD355 pKa = 3.79HH356 pKa = 6.84PGEE359 pKa = 4.32LVFSLGVTADD369 pKa = 3.52QVASGLYY376 pKa = 10.67ANDD379 pKa = 3.72PSAVDD384 pKa = 3.6GKK386 pKa = 10.97GVEE389 pKa = 3.85IQLIEE394 pKa = 4.02EE395 pKa = 4.5GGAIRR400 pKa = 11.84GIAGGIEE407 pKa = 4.44YY408 pKa = 8.03FTISIDD414 pKa = 3.93SNSGEE419 pKa = 3.98MTFAQSEE426 pKa = 4.85AIWHH430 pKa = 6.03GNPANPDD437 pKa = 3.55DD438 pKa = 4.48LASLNTPDD446 pKa = 5.06INDD449 pKa = 3.29LFIRR453 pKa = 11.84VTAIDD458 pKa = 3.81GDD460 pKa = 4.08GDD462 pKa = 3.74KK463 pKa = 11.15ASYY466 pKa = 10.29QLGLGQGIFSINDD479 pKa = 3.74DD480 pKa = 4.26GPGIDD485 pKa = 4.31PGLIVAADD493 pKa = 4.04PLNVDD498 pKa = 3.83EE499 pKa = 5.17TNLATGNAVATANYY513 pKa = 10.24ADD515 pKa = 4.1NFQPAIDD522 pKa = 4.07YY523 pKa = 11.25GEE525 pKa = 5.08DD526 pKa = 3.56GAGQTVYY533 pKa = 10.55EE534 pKa = 4.62LSLNGQPGSGLYY546 pKa = 10.67AIDD549 pKa = 4.2PNQANGQGDD558 pKa = 4.88EE559 pKa = 4.18IQLVQVDD566 pKa = 3.64SHH568 pKa = 5.52TVQGQVNGEE577 pKa = 4.23TYY579 pKa = 9.16FTVTVDD585 pKa = 3.17GGTGEE590 pKa = 4.1VTFAQNRR597 pKa = 11.84AVWHH601 pKa = 6.42SDD603 pKa = 2.81SANANDD609 pKa = 5.23LSTLTTANADD619 pKa = 3.58DD620 pKa = 3.73FTIRR624 pKa = 11.84AIVTDD629 pKa = 3.79ADD631 pKa = 3.88GDD633 pKa = 4.41SVEE636 pKa = 4.11QALNLGQGTFSINDD650 pKa = 3.96DD651 pKa = 3.8GPLVNGNTDD660 pKa = 3.47TAQLVYY666 pKa = 10.84EE667 pKa = 4.66DD668 pKa = 5.24ALPAGNADD676 pKa = 3.55PGQTLNISGSLSSLVDD692 pKa = 4.45FGADD696 pKa = 3.24GPGGFEE702 pKa = 4.21LTTDD706 pKa = 3.76YY707 pKa = 10.4LTTLAAFGLTSDD719 pKa = 4.35GQALSYY725 pKa = 10.45SASEE729 pKa = 4.02NNGIQSVVAKK739 pKa = 10.13DD740 pKa = 3.65YY741 pKa = 11.17LGQDD745 pKa = 3.05VFTFTFDD752 pKa = 3.36KK753 pKa = 9.09TTGNYY758 pKa = 10.55DD759 pKa = 3.19FTLQSHH765 pKa = 7.35LDD767 pKa = 3.7HH768 pKa = 7.11LPPPIAQDD776 pKa = 4.64DD777 pKa = 4.19DD778 pKa = 4.35TQSLPIQLGAIINAIDD794 pKa = 3.85ADD796 pKa = 4.02GDD798 pKa = 4.16KK799 pKa = 11.53VNLGQSFGVHH809 pKa = 5.11VQDD812 pKa = 5.74DD813 pKa = 4.47LPVAQNDD820 pKa = 3.92SVSVNEE826 pKa = 4.85PNAQLNIYY834 pKa = 9.36YY835 pKa = 10.13VVDD838 pKa = 3.46VSGSMGVVDD847 pKa = 4.54PLIGQSRR854 pKa = 11.84LEE856 pKa = 4.07LARR859 pKa = 11.84EE860 pKa = 3.88ALVNLTQEE868 pKa = 4.09YY869 pKa = 10.77ANYY872 pKa = 9.54NGEE875 pKa = 4.15VFVTVVPFASGSGNSGAFATAKK897 pKa = 10.21FDD899 pKa = 3.63ILHH902 pKa = 6.59GEE904 pKa = 4.33LVDD907 pKa = 3.78ALNFIGNLQVSQNPIPGSGTEE928 pKa = 4.2YY929 pKa = 11.25NDD931 pKa = 4.24ALDD934 pKa = 3.75VTQDD938 pKa = 3.61NLEE941 pKa = 4.44LDD943 pKa = 4.73LNPLSQVAGYY953 pKa = 10.31QNVVYY958 pKa = 9.54FLSDD962 pKa = 3.84GVPVPASAGAPAGWQSFVDD981 pKa = 3.71TNNIDD986 pKa = 3.77VIPIGIGDD994 pKa = 4.58FNPGPLEE1001 pKa = 4.05EE1002 pKa = 4.58VGNTGDD1008 pKa = 3.72TVIQVLDD1015 pKa = 3.81PTDD1018 pKa = 3.84LSSTLIEE1025 pKa = 3.97QLDD1028 pKa = 3.81NVVQGNVLDD1037 pKa = 4.08NDD1039 pKa = 3.88AFGGDD1044 pKa = 3.99GPDD1047 pKa = 3.16VPSIVSVSYY1056 pKa = 10.5QGVDD1060 pKa = 2.83HH1061 pKa = 7.73DD1062 pKa = 4.84YY1063 pKa = 11.62DD1064 pKa = 3.78GVNNSVTIIDD1074 pKa = 4.09DD1075 pKa = 3.6FGGRR1079 pKa = 11.84LTFDD1083 pKa = 3.9FTSGDD1088 pKa = 3.53YY1089 pKa = 10.9SYY1091 pKa = 10.83IAPGVDD1097 pKa = 2.93QDD1099 pKa = 3.9EE1100 pKa = 4.41QVQFQYY1106 pKa = 10.68TIRR1109 pKa = 11.84DD1110 pKa = 3.42NDD1112 pKa = 3.74GDD1114 pKa = 4.36LSTANLNIVVNHH1126 pKa = 7.07LDD1128 pKa = 3.85PPSVTLLTKK1137 pKa = 9.74TLEE1140 pKa = 4.21FTRR1143 pKa = 11.84TEE1145 pKa = 4.04QGQQPYY1151 pKa = 10.67GNADD1155 pKa = 3.14VAEE1158 pKa = 4.08YY1159 pKa = 11.29LLLQALGNEE1168 pKa = 4.53SQQVTSVPINGVNLSNTFISVATEE1192 pKa = 3.54PTVNFVSEE1200 pKa = 4.17GAGYY1204 pKa = 10.67RR1205 pKa = 11.84NMVGYY1210 pKa = 7.87YY1211 pKa = 9.95TYY1213 pKa = 11.28DD1214 pKa = 2.92SDD1216 pKa = 5.87GNINDD1221 pKa = 4.91AGFIWVNSSASSSAQNPNGGTNLITNTSGAFGFNQSASFTLPDD1264 pKa = 3.35VSAGEE1269 pKa = 4.01GLGFFLLADD1278 pKa = 4.6GANDD1282 pKa = 3.6SANVNIIRR1290 pKa = 11.84NGNYY1294 pKa = 10.39DD1295 pKa = 4.32SIQSLNNNVSIVNNQVLVGGQALNGSIYY1323 pKa = 9.23FTHH1326 pKa = 7.77DD1327 pKa = 2.56KK1328 pKa = 10.11TLNSDD1333 pKa = 3.51YY1334 pKa = 11.01ATFPEE1339 pKa = 4.45GHH1341 pKa = 7.02ALSGVTFSQDD1351 pKa = 2.99PSLTAYY1357 pKa = 10.39SGLLIIGFEE1366 pKa = 4.23DD1367 pKa = 3.47VHH1369 pKa = 8.99DD1370 pKa = 5.78GGDD1373 pKa = 3.1QDD1375 pKa = 4.22YY1376 pKa = 10.84NDD1378 pKa = 4.28VIFSVDD1384 pKa = 3.49LGANIQNYY1392 pKa = 7.86ATDD1395 pKa = 3.54SSPIIEE1401 pKa = 4.83SVTDD1405 pKa = 3.75PDD1407 pKa = 3.87SAYY1410 pKa = 9.28LTKK1413 pKa = 10.75AVVTTDD1419 pKa = 3.18GFIEE1423 pKa = 5.02GDD1425 pKa = 3.63TLNFLSNANVQFDD1438 pKa = 4.01TVNNQVTVTYY1448 pKa = 10.71NGADD1452 pKa = 2.97HH1453 pKa = 6.34VLNYY1457 pKa = 10.17TLTPDD1462 pKa = 4.02GNDD1465 pKa = 3.13PDD1467 pKa = 5.3FIYY1470 pKa = 10.38TFAAADD1476 pKa = 3.55VNNPVPVGAYY1486 pKa = 9.66EE1487 pKa = 3.98MALKK1491 pKa = 10.37SISFTPVASEE1501 pKa = 3.67ASEE1504 pKa = 4.28GVARR1508 pKa = 11.84DD1509 pKa = 3.58VYY1511 pKa = 10.49VTVTDD1516 pKa = 3.83DD1517 pKa = 3.38TGLSATQATSFNVVINDD1534 pKa = 4.06ILPMDD1539 pKa = 5.22LLAFDD1544 pKa = 4.04QQNEE1548 pKa = 3.91FDD1550 pKa = 4.55FGQGNDD1556 pKa = 3.36TVLIPDD1562 pKa = 3.44QMDD1565 pKa = 3.41GLGQVSGGQGIDD1577 pKa = 3.04TVKK1580 pKa = 9.77FTEE1583 pKa = 3.94EE1584 pKa = 3.95GMNLTAEE1591 pKa = 5.05EE1592 pKa = 4.28IQDD1595 pKa = 3.6HH1596 pKa = 6.9FNGHH1600 pKa = 5.73WEE1602 pKa = 4.14QIDD1605 pKa = 3.6MTEE1608 pKa = 3.84QGSNILNLDD1617 pKa = 3.84LEE1619 pKa = 4.83AVLKK1623 pKa = 10.72LSSGSEE1629 pKa = 4.35LNLQLNNGEE1638 pKa = 4.33NVSVLQIKK1646 pKa = 10.61ADD1648 pKa = 3.87GTDD1651 pKa = 3.6SPDD1654 pKa = 3.44GSVDD1658 pKa = 3.4TVNIDD1663 pKa = 3.49SAHH1666 pKa = 5.14VTDD1669 pKa = 6.13VSDD1672 pKa = 3.67QLPAAGQTAAEE1683 pKa = 4.13QLFKK1687 pKa = 10.52LTNADD1692 pKa = 3.56ASQTAYY1698 pKa = 9.98VHH1700 pKa = 6.04VVSLTQQLPEE1710 pKa = 3.9VHH1712 pKa = 6.09ATSTDD1717 pKa = 3.14GGGGGGGGGG1726 pKa = 3.4

Molecular weight: 181.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0W0Z964|A0A0W0Z964_LEGSP UPF0434 protein Lspi_0355 OS=Legionella spiritensis OX=452 GN=lpxK_1 PE=3 SV=1
MM1 pKa = 8.03RR2 pKa = 11.84DD3 pKa = 3.6NVPNWHH9 pKa = 6.99IKK11 pKa = 9.07PAIRR15 pKa = 11.84PQASLRR21 pKa = 11.84ATAFSQKK28 pKa = 10.41NGANYY33 pKa = 9.67PHH35 pKa = 6.67FRR37 pKa = 11.84SAKK40 pKa = 8.93IFVHH44 pKa = 5.51RR45 pKa = 11.84TWKK48 pKa = 10.82AGIFCAAANCLANTKK63 pKa = 10.12SSAVLISCNGGTQACSTRR81 pKa = 11.84ILL83 pKa = 3.65

Molecular weight: 9.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2908

0

2908

978053

50

6909

336.3

37.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.976 ± 0.041

1.204 ± 0.016

5.254 ± 0.034

5.735 ± 0.045

4.372 ± 0.032

6.345 ± 0.041

2.622 ± 0.024

6.938 ± 0.038

5.575 ± 0.043

10.904 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.524 ± 0.021

4.425 ± 0.03

4.222 ± 0.025

4.573 ± 0.036

5.039 ± 0.032

6.282 ± 0.035

5.243 ± 0.031

6.191 ± 0.046

1.186 ± 0.018

3.388 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski