Streptococcus phage Javan188
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6ATL2|A0A4D6ATL2_9CAUD Uncharacterized protein OS=Streptococcus phage Javan188 OX=2548036 GN=Javan188_0053 PE=4 SV=1
MM1 pKa = 7.64 NYY3 pKa = 10.43 LEE5 pKa = 4.23 YY6 pKa = 11.18 ALVYY10 pKa = 10.03 LEE12 pKa = 5.12 RR13 pKa = 11.84 EE14 pKa = 4.11 LEE16 pKa = 4.5 IIDD19 pKa = 3.9 NEE21 pKa = 4.38 VIEE24 pKa = 4.3 VEE26 pKa = 4.36 LPGGDD31 pKa = 3.23 WEE33 pKa = 4.69 FVPNPYY39 pKa = 9.61 YY40 pKa = 10.61 EE41 pKa = 5.06 KK42 pKa = 10.67 GLHH45 pKa = 6.74 DD46 pKa = 4.15 SPHH49 pKa = 5.83 YY50 pKa = 9.94 RR51 pKa = 11.84 SQVAKK56 pKa = 10.58 DD57 pKa = 3.09 ILDD60 pKa = 3.46 IKK62 pKa = 10.8 GLLGRR67 pKa = 4.72
Molecular weight: 7.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.317
IPC2_protein 4.495
IPC_protein 4.355
Toseland 4.202
ProMoST 4.457
Dawson 4.291
Bjellqvist 4.444
Wikipedia 4.164
Rodwell 4.19
Grimsley 4.126
Solomon 4.291
Lehninger 4.24
Nozaki 4.418
DTASelect 4.533
Thurlkill 4.215
EMBOSS 4.177
Sillero 4.469
Patrickios 3.058
IPC_peptide 4.291
IPC2_peptide 4.457
IPC2.peptide.svr19 4.392
Protein with the highest isoelectric point:
>tr|A0A4D6AV18|A0A4D6AV18_9CAUD Terminase small subunit OS=Streptococcus phage Javan188 OX=2548036 GN=Javan188_0029 PE=4 SV=1
MM1 pKa = 7.78 RR2 pKa = 11.84 GLDD5 pKa = 3.09 RR6 pKa = 11.84 FLRR9 pKa = 11.84 SVEE12 pKa = 4.05 RR13 pKa = 11.84 KK14 pKa = 8.65 QKK16 pKa = 9.38 SVRR19 pKa = 11.84 IAVDD23 pKa = 3.31 KK24 pKa = 10.73 EE25 pKa = 4.09 LSKK28 pKa = 10.9 SAARR32 pKa = 11.84 IEE34 pKa = 4.15 RR35 pKa = 11.84 QAKK38 pKa = 9.19 ILAPVDD44 pKa = 3.94 TGWLRR49 pKa = 11.84 AQIYY53 pKa = 10.15 SEE55 pKa = 4.1 QQRR58 pKa = 11.84 LLHH61 pKa = 5.57 YY62 pKa = 9.97 RR63 pKa = 11.84 VVSPALYY70 pKa = 10.27 SIYY73 pKa = 10.79 LEE75 pKa = 4.28 LGTRR79 pKa = 11.84 KK80 pKa = 8.85 MEE82 pKa = 4.3 AQSFLDD88 pKa = 3.47 PALRR92 pKa = 11.84 KK93 pKa = 8.48 EE94 pKa = 4.46 WPVLMANIKK103 pKa = 10.68 KK104 pKa = 8.93 MFKK107 pKa = 10.27 RR108 pKa = 3.68
Molecular weight: 12.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.299
IPC2_protein 9.75
IPC_protein 10.394
Toseland 10.745
ProMoST 10.526
Dawson 10.833
Bjellqvist 10.496
Wikipedia 11.008
Rodwell 11.169
Grimsley 10.877
Solomon 10.921
Lehninger 10.891
Nozaki 10.701
DTASelect 10.496
Thurlkill 10.73
EMBOSS 11.125
Sillero 10.745
Patrickios 10.906
IPC_peptide 10.921
IPC2_peptide 9.136
IPC2.peptide.svr19 8.745
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
12761
38
1215
232.0
26.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.813 ± 0.555
0.423 ± 0.101
6.214 ± 0.384
7.264 ± 0.511
3.769 ± 0.237
6.206 ± 0.385
1.293 ± 0.141
7.162 ± 0.328
8.683 ± 0.475
8.894 ± 0.32
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.343 ± 0.152
5.47 ± 0.256
2.829 ± 0.182
3.848 ± 0.238
4.349 ± 0.307
6.653 ± 0.584
5.854 ± 0.295
5.956 ± 0.174
1.034 ± 0.126
3.942 ± 0.376
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here