Lutibacter sp. Hel_I_33_5
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2634 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A559RYQ8|A0A559RYQ8_9FLAO Uncharacterized protein OS=Lutibacter sp. Hel_I_33_5 OX=1566289 GN=OD91_0488 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.52 NNYY5 pKa = 9.87 SKK7 pKa = 11.13 FILKK11 pKa = 8.86 TLLLFAILFGLEE23 pKa = 3.88 TYY25 pKa = 10.01 AQQNIIAQTQNITVQLDD42 pKa = 3.56 ANGEE46 pKa = 4.25 AIITPQQINNGSTATTGIQSLNLDD70 pKa = 3.62 TSDD73 pKa = 5.29 FNCTDD78 pKa = 2.94 INGDD82 pKa = 3.69 RR83 pKa = 11.84 NFSYY87 pKa = 11.0 LLDD90 pKa = 4.5 GEE92 pKa = 4.49 TDD94 pKa = 3.23 ATGDD98 pKa = 4.04 EE99 pKa = 4.34 ILVDD103 pKa = 3.93 DD104 pKa = 4.79 TNLTTLTTQTIEE116 pKa = 3.44 AWIRR120 pKa = 11.84 PATGASDD127 pKa = 4.0 NDD129 pKa = 3.31 VLVAVRR135 pKa = 11.84 NNIHH139 pKa = 6.24 NSRR142 pKa = 11.84 FSIHH146 pKa = 6.62 INADD150 pKa = 3.29 TNTIGIFNSTNWQTITTIINANQWYY175 pKa = 9.43 HH176 pKa = 6.08 IALVMNSTKK185 pKa = 10.36 TLVYY189 pKa = 10.51 LDD191 pKa = 3.92 GAFEE195 pKa = 4.68 GEE197 pKa = 3.9 ISTGINTSLGANAPLSIGSPNDD219 pKa = 3.44 TGWYY223 pKa = 7.61 SEE225 pKa = 3.87 KK226 pKa = 10.41 WEE228 pKa = 4.36 GNIDD232 pKa = 5.46 DD233 pKa = 4.21 LRR235 pKa = 11.84 IWNDD239 pKa = 2.44 EE240 pKa = 3.95 RR241 pKa = 11.84 TALEE245 pKa = 3.64 IANNRR250 pKa = 11.84 NIEE253 pKa = 4.11 LVGNEE258 pKa = 4.22 AGLVGYY264 pKa = 10.48 FDD266 pKa = 5.05 FNEE269 pKa = 4.58 GTGNTITNLAGTVNGTANNSQNANWSTDD297 pKa = 3.14 SPIEE301 pKa = 4.42 SNGNTVNLTVTDD313 pKa = 3.86 VNGNSATTPANVIVVDD329 pKa = 5.01 NIAPIAPTLADD340 pKa = 3.43 VTGEE344 pKa = 4.02 CSATVTAPTTTDD356 pKa = 2.77 NCAGTITGTTTDD368 pKa = 3.32 SLTYY372 pKa = 7.42 TTQGTHH378 pKa = 6.68 IITWNFDD385 pKa = 3.58 DD386 pKa = 5.63 GNGNSTTASQNVIISDD402 pKa = 3.98 STPPTAITNNISVNLDD418 pKa = 3.23 ANGNASITPQQIDD431 pKa = 3.55 NGSSDD436 pKa = 3.43 ACGISSISCSRR447 pKa = 11.84 VGGSGNGLTNFLNGTLNNGTADD469 pKa = 3.21 IIEE472 pKa = 4.33 YY473 pKa = 10.71 SNSNAPNYY481 pKa = 7.41 RR482 pKa = 11.84 TSFGTTCNEE491 pKa = 3.02 AWFARR496 pKa = 11.84 GMLCDD501 pKa = 4.66 LAPNQNDD508 pKa = 3.73 PDD510 pKa = 3.71 NSSRR514 pKa = 11.84 QDD516 pKa = 3.41 NNAGATWRR524 pKa = 11.84 NTSPGNSYY532 pKa = 10.56 GRR534 pKa = 11.84 LVIDD538 pKa = 4.97 LGSSQTINAMSIFQMFSDD556 pKa = 4.19 GKK558 pKa = 6.81 TTHH561 pKa = 6.72 IEE563 pKa = 3.62 AFAYY567 pKa = 10.21 NGNLEE572 pKa = 4.36 NPNSSSPGWNQLFPSTSVGAGVLNGTTVSSPLKK605 pKa = 8.72 TTFQSTNTRR614 pKa = 11.84 FIKK617 pKa = 10.58 LYY619 pKa = 10.44 AKK621 pKa = 10.52 NDD623 pKa = 3.28 GSLGFSGYY631 pKa = 9.92 IEE633 pKa = 4.04 LRR635 pKa = 11.84 QVKK638 pKa = 8.98 VFNTNEE644 pKa = 3.77 SNACDD649 pKa = 4.49 FNCDD653 pKa = 3.79 DD654 pKa = 4.63 LGEE657 pKa = 4.41 NNVTLTVEE665 pKa = 4.49 DD666 pKa = 3.87 KK667 pKa = 11.19 NGNISTKK674 pKa = 10.5 DD675 pKa = 3.51 AIVTIIDD682 pKa = 4.82 AIAPALEE689 pKa = 4.32 ATPADD694 pKa = 3.89 LTVEE698 pKa = 4.25 CHH700 pKa = 6.73 SIPDD704 pKa = 3.56 VATISATDD712 pKa = 3.38 NCDD715 pKa = 3.33 SNISVNFTEE724 pKa = 4.09 QRR726 pKa = 11.84 ADD728 pKa = 3.23 GFSNDD733 pKa = 3.57 NYY735 pKa = 10.28 TLTRR739 pKa = 11.84 TWSATDD745 pKa = 3.21 QSGNTVSEE753 pKa = 4.39 SQVITVQDD761 pKa = 3.69 VTPPTIVGQNISVTLTASGTVSIIPQNVLNNGSDD795 pKa = 3.37 TCSTVSYY802 pKa = 9.46 TISQNTFGATDD813 pKa = 5.5 AINSPVTIQLIGTDD827 pKa = 3.51 EE828 pKa = 4.43 SGNATTIPVQVTVIDD843 pKa = 4.05 PVPVVITQNIVIQLDD858 pKa = 3.72 DD859 pKa = 4.13 NGTVVIDD866 pKa = 4.16 PLQIDD871 pKa = 4.14 NGSSSAVGIGSLSLDD886 pKa = 2.95 ISAFNCEE893 pKa = 4.91 DD894 pKa = 3.06 INNPVTVTLTVTSTLGSSSTGTAIVTVQDD923 pKa = 4.35 TIAPIAPILSDD934 pKa = 3.4 ILGEE938 pKa = 4.32 CSATATTPTTTDD950 pKa = 2.7 NCAGSITGTTTDD962 pKa = 3.28 SLEE965 pKa = 3.87 YY966 pKa = 9.72 TIQGTHH972 pKa = 6.87 TITWSFDD979 pKa = 3.3 DD980 pKa = 4.83 GNGNTTTATQNVIVNDD996 pKa = 3.79 TTAPIAITKK1005 pKa = 10.33 NITIPLDD1012 pKa = 3.44 ASGNAIITPQMINNGSSDD1030 pKa = 3.3 NCEE1033 pKa = 3.68 IDD1035 pKa = 5.75 SITLDD1040 pKa = 3.29 NTVFDD1045 pKa = 4.81 CSNVDD1050 pKa = 4.98 SSAQCNAGTALDD1062 pKa = 4.49 LGADD1066 pKa = 3.46 NDD1068 pKa = 4.04 YY1069 pKa = 11.29 VRR1071 pKa = 11.84 IGDD1074 pKa = 3.91 PFHH1077 pKa = 6.99 EE1078 pKa = 5.16 FSNQITVSWWVNFQGNSAWMTQSTFGMSSNNTNVWNMHH1116 pKa = 5.82 ASGANGITWYY1126 pKa = 10.32 INDD1129 pKa = 3.46 NGAYY1133 pKa = 9.84 RR1134 pKa = 11.84 AVSLNTTAGWHH1145 pKa = 6.95 LITGVADD1152 pKa = 3.7 TTSTRR1157 pKa = 11.84 LYY1159 pKa = 10.84 LDD1161 pKa = 4.1 GNLASTDD1168 pKa = 3.27 TGINNIRR1175 pKa = 11.84 VNANSEE1181 pKa = 3.74 IAIGIDD1187 pKa = 3.17 GRR1189 pKa = 11.84 IPSRR1193 pKa = 11.84 NRR1195 pKa = 11.84 NFQTDD1200 pKa = 3.03 EE1201 pKa = 3.72 VLIYY1205 pKa = 10.87 NRR1207 pKa = 11.84 ALSEE1211 pKa = 4.27 TEE1213 pKa = 3.88 IQDD1216 pKa = 5.18 LLMNCPDD1223 pKa = 3.4 ISDD1226 pKa = 3.87 NSLVGYY1232 pKa = 9.13 WAMNEE1237 pKa = 4.05 GSGNTIEE1244 pKa = 4.74 DD1245 pKa = 3.53 QSITGANGVLINMTDD1260 pKa = 3.79 ADD1262 pKa = 4.18 WVTSGINNIGAGSSNEE1278 pKa = 3.83 VTLTVTDD1285 pKa = 3.74 VNGNSSTAIANVTVNDD1301 pKa = 3.33 ITAPTLNDD1309 pKa = 2.9 VPANVTVEE1317 pKa = 4.06 CDD1319 pKa = 4.2 AIPNAATISASDD1331 pKa = 3.36 NCGNPTITFVEE1342 pKa = 4.06 EE1343 pKa = 4.47 RR1344 pKa = 11.84 IDD1346 pKa = 4.12 GKK1348 pKa = 11.74 SNDD1351 pKa = 3.61 NYY1353 pKa = 10.36 TLNRR1357 pKa = 11.84 TWTATDD1363 pKa = 3.2 QSEE1366 pKa = 4.21 NSVSHH1371 pKa = 4.98 TQIITVQDD1379 pKa = 3.28 TTAPFFDD1386 pKa = 4.3 ATPVDD1391 pKa = 3.37 ITVEE1395 pKa = 4.23 CEE1397 pKa = 4.11 TVPAAATISASDD1409 pKa = 3.36 NCGNPTITFVEE1420 pKa = 4.15 EE1421 pKa = 4.48 RR1422 pKa = 11.84 IDD1424 pKa = 5.03 GISNDD1429 pKa = 3.53 NYY1431 pKa = 10.17 TLTRR1435 pKa = 11.84 TWTATDD1441 pKa = 3.92 LSGNTVSEE1449 pKa = 4.28 SQIITVQDD1457 pKa = 3.22 VTAPTVVGQNISVTLTSTGTVSIVPEE1483 pKa = 3.92 NVLDD1487 pKa = 4.39 NGSDD1491 pKa = 3.26 NCGTVTYY1498 pKa = 10.24 SVSQDD1503 pKa = 2.96 TFGATDD1509 pKa = 3.57 AVNSPVTVQLTGTDD1523 pKa = 3.24 ASGNATTVPVLVTVIDD1539 pKa = 4.01 PVPVVITQNIVVQLDD1554 pKa = 3.65 DD1555 pKa = 4.12 NGTVVIDD1562 pKa = 4.31 PSQIDD1567 pKa = 3.71 NGSSSAVGIGSLSLDD1582 pKa = 2.95 ISAFNCEE1589 pKa = 4.91 DD1590 pKa = 3.06 INNPVTVTLTVTSTLGSSSTGTAIVTVQDD1619 pKa = 4.35 TIAPIAPILSDD1630 pKa = 3.4 ILGEE1634 pKa = 4.32 CSATATTPTTTDD1646 pKa = 2.7 NCAGSITGTTTDD1658 pKa = 3.66 YY1659 pKa = 11.67 LEE1661 pKa = 4.18 YY1662 pKa = 8.84 TAQGIHH1668 pKa = 6.29 VITWSFDD1675 pKa = 3.44 DD1676 pKa = 4.34 GNGNIATATQNVIVIDD1692 pKa = 3.78 VTAPTAIAQDD1702 pKa = 3.25 ISINLDD1708 pKa = 3.4 DD1709 pKa = 5.21 NGNATITPQQIDD1721 pKa = 3.68 NDD1723 pKa = 4.42 SNDD1726 pKa = 3.21 ACGIASTSLDD1736 pKa = 3.95 KK1737 pKa = 11.66 NSFDD1741 pKa = 4.0 CSNVGSSASSSSFSFDD1757 pKa = 3.76 GIANANGDD1765 pKa = 4.38 EE1766 pKa = 4.1 ILVNDD1771 pKa = 4.38 TSFSTLTAQTIEE1783 pKa = 3.66 AWIRR1787 pKa = 11.84 PASGSNGNEE1796 pKa = 3.24 SWLAIRR1802 pKa = 11.84 DD1803 pKa = 3.56 RR1804 pKa = 11.84 VGNSRR1809 pKa = 11.84 YY1810 pKa = 10.11 SIHH1813 pKa = 6.77 TNPSANTIGVYY1824 pKa = 10.58 NSTHH1828 pKa = 6.72 GYY1830 pKa = 7.49 RR1831 pKa = 11.84 TISISTGINANQWYY1845 pKa = 8.62 HH1846 pKa = 5.27 VAMVMNTTRR1855 pKa = 11.84 TLVYY1859 pKa = 11.04 VNGTLAGQIAAGISTNHH1876 pKa = 6.56 GANVPLSIGSPNDD1889 pKa = 3.02 ASYY1892 pKa = 11.4 YY1893 pKa = 8.15 GEE1895 pKa = 4.09 KK1896 pKa = 9.11 WQGDD1900 pKa = 3.83 MDD1902 pKa = 5.85 DD1903 pKa = 3.8 LRR1905 pKa = 11.84 VWNTEE1910 pKa = 3.45 RR1911 pKa = 11.84 TATEE1915 pKa = 3.55 IADD1918 pKa = 3.58 NRR1920 pKa = 11.84 NNEE1923 pKa = 3.97 LTGNEE1928 pKa = 4.04 VGLVGYY1934 pKa = 9.75 FNFNEE1939 pKa = 4.43 GSGNSVDD1946 pKa = 4.29 NLAGTINGTANNSQTEE1962 pKa = 3.99 NWIDD1966 pKa = 3.59 EE1967 pKa = 4.48 SPISGSTGNMVTLTVTDD1984 pKa = 3.69 VNGNSSTATANVTVVDD2000 pKa = 4.61 NISPEE2005 pKa = 3.82 ISDD2008 pKa = 3.81 PADD2011 pKa = 3.04 INVFATSAAGAVVNYY2026 pKa = 6.75 NTPIGTDD2033 pKa = 2.93 NCTVTTALTAGLADD2047 pKa = 4.27 GEE2049 pKa = 4.6 TFPIGTTVVSYY2060 pKa = 8.75 TATDD2064 pKa = 3.93 GSGNTATATFNVIVTGLPPEE2084 pKa = 4.1 IVVPEE2089 pKa = 5.1 DD2090 pKa = 2.9 ITVNNDD2096 pKa = 2.76 SGEE2099 pKa = 4.17 CGAIVNYY2106 pKa = 10.33 AATEE2110 pKa = 4.26 TVGIPASTITYY2121 pKa = 9.62 DD2122 pKa = 3.37 IEE2124 pKa = 4.8 PGSFFAEE2131 pKa = 4.37 GTVTVTATATNPVGTSTKK2149 pKa = 9.99 TFTVTVNDD2157 pKa = 3.56 NQLPSVLTQNLIINLDD2173 pKa = 3.66 ANGNSTITPQQIDD2186 pKa = 3.48 NGSSDD2191 pKa = 3.35 NCGIASISLDD2201 pKa = 3.17 NDD2203 pKa = 3.54 TFNCSNVSTNTVTLTVTDD2221 pKa = 3.58 VNGNSNSLTANVTVNDD2237 pKa = 3.37 VTAPNVITQNIIIEE2251 pKa = 4.09 LDD2253 pKa = 3.62 EE2254 pKa = 4.78 NGEE2257 pKa = 4.01 ASITPADD2264 pKa = 3.7 IDD2266 pKa = 4.01 NNSTDD2271 pKa = 2.92 ACGIEE2276 pKa = 4.97 SYY2278 pKa = 11.34 SLDD2281 pKa = 3.99 KK2282 pKa = 11.44 DD2283 pKa = 4.41 SFDD2286 pKa = 5.21 CSNTGDD2292 pKa = 3.63 NTVVLTVTDD2301 pKa = 3.68 VNGNDD2306 pKa = 3.45 ASLSATVTVQDD2317 pKa = 4.41 NIAPTVITQSYY2328 pKa = 10.47 SIDD2331 pKa = 3.7 LTNGVADD2338 pKa = 5.34 IIPSNIDD2345 pKa = 2.9 GGTFDD2350 pKa = 4.65 NCDD2353 pKa = 3.19 FTLSIDD2359 pKa = 3.87 RR2360 pKa = 11.84 DD2361 pKa = 3.81 HH2362 pKa = 6.59 FTCDD2366 pKa = 3.82 DD2367 pKa = 3.63 IGDD2370 pKa = 4.09 HH2371 pKa = 6.21 VVTLTATDD2379 pKa = 3.49 ASGNSSSEE2387 pKa = 3.98 TAIVSILGNVPTIGINDD2404 pKa = 3.57 FNIVQTQKK2412 pKa = 11.22 KK2413 pKa = 7.03 NTIFLGFGPQSINLSTVVDD2432 pKa = 4.16 GGSGFTYY2439 pKa = 10.38 EE2440 pKa = 4.12 WTTSTGEE2447 pKa = 4.27 VVSNQANPTINPTVSTTYY2465 pKa = 10.86 YY2466 pKa = 9.36 VTVTNANGCTASTSIYY2482 pKa = 10.2 VCVIDD2487 pKa = 4.8 ARR2489 pKa = 11.84 AYY2491 pKa = 9.95 DD2492 pKa = 3.28 RR2493 pKa = 11.84 KK2494 pKa = 10.5 GRR2496 pKa = 11.84 YY2497 pKa = 7.95 KK2498 pKa = 10.86 GKK2500 pKa = 10.51 VLVCHH2505 pKa = 5.99 HH2506 pKa = 6.69 TNGKK2510 pKa = 9.92 KK2511 pKa = 8.63 GTKK2514 pKa = 9.44 HH2515 pKa = 5.3 VMISISKK2522 pKa = 8.21 NAVMQHH2528 pKa = 5.13 LTKK2531 pKa = 10.67 HH2532 pKa = 6.45 GIGTDD2537 pKa = 3.36 HH2538 pKa = 7.49 ADD2540 pKa = 4.06 SLGACNAQCVTNQTILARR2558 pKa = 11.84 TKK2560 pKa = 9.87 TINLSSVTTYY2570 pKa = 10.43 PNPSNGIFEE2579 pKa = 4.38 VRR2581 pKa = 11.84 LSKK2584 pKa = 11.4 AEE2586 pKa = 4.21 DD2587 pKa = 3.33 NTRR2590 pKa = 11.84 LLLFDD2595 pKa = 4.2 LTGKK2599 pKa = 10.77 LILHH2603 pKa = 5.89 KK2604 pKa = 9.7 TISTKK2609 pKa = 9.9 KK2610 pKa = 10.79 ASMGSNNLPSGVYY2623 pKa = 8.69 ILKK2626 pKa = 10.52 VIYY2629 pKa = 10.2 NDD2631 pKa = 3.99 SISTKK2636 pKa = 10.53 KK2637 pKa = 10.84 LIIEE2641 pKa = 4.28 KK2642 pKa = 10.67 GKK2644 pKa = 10.69
Molecular weight: 277.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.757
IPC_protein 3.821
Toseland 3.579
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.643
Grimsley 3.49
Solomon 3.821
Lehninger 3.77
Nozaki 3.923
DTASelect 4.215
Thurlkill 3.63
EMBOSS 3.77
Sillero 3.948
Patrickios 1.125
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.826
Protein with the highest isoelectric point:
>tr|A0A559S2L1|A0A559S2L1_9FLAO NTE family protein OS=Lutibacter sp. Hel_I_33_5 OX=1566289 GN=OD91_1881 PE=4 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.34 KK6 pKa = 9.56 RR7 pKa = 11.84 KK8 pKa = 8.34 RR9 pKa = 11.84 AKK11 pKa = 10.25 ISTHH15 pKa = 4.55 KK16 pKa = 9.9 RR17 pKa = 11.84 KK18 pKa = 9.85 KK19 pKa = 8.28 RR20 pKa = 11.84 ARR22 pKa = 11.84 ANRR25 pKa = 11.84 HH26 pKa = 4.7 KK27 pKa = 10.78 KK28 pKa = 9.39 KK29 pKa = 10.83
Molecular weight: 3.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.023
IPC_protein 12.544
Toseland 12.72
ProMoST 13.203
Dawson 12.735
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.676
Grimsley 12.764
Solomon 13.203
Lehninger 13.115
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.398
IPC_peptide 13.217
IPC2_peptide 12.193
IPC2.peptide.svr19 9.0
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2634
0
2634
952811
29
9603
361.7
40.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.861 ± 0.064
0.705 ± 0.017
5.787 ± 0.181
6.153 ± 0.08
5.327 ± 0.082
6.394 ± 0.097
1.607 ± 0.03
8.418 ± 0.084
8.647 ± 0.152
8.935 ± 0.087
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.872 ± 0.034
7.012 ± 0.068
3.128 ± 0.046
3.042 ± 0.035
3.002 ± 0.059
6.817 ± 0.054
6.335 ± 0.186
6.103 ± 0.049
0.962 ± 0.019
3.895 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here