Candidatus Accumulibacter sp. BA-94

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Betaproteobacteria incertae sedis; Candidatus Accumulibacter; unclassified Candidatus Accumulibacter

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A011Q045|A0A011Q045_9PROT Uncharacterized protein OS=Candidatus Accumulibacter sp. BA-94 OX=1454005 GN=AW12_00080 PE=4 SV=1
MM1 pKa = 7.48LVNDD5 pKa = 4.51TGTGLSIFQTSPPAHH20 pKa = 6.44GSATISGGAITYY32 pKa = 7.6TPSAGFAGTDD42 pKa = 3.3SFSYY46 pKa = 9.8TIRR49 pKa = 11.84DD50 pKa = 3.32SFGQSASATVTVTVTVTVTVTAVPINPPGLSAVDD84 pKa = 3.99DD85 pKa = 4.34AVSTVAGNPVTINVLTNDD103 pKa = 3.69TGTGLDD109 pKa = 3.8IIQVSRR115 pKa = 11.84PAHH118 pKa = 5.81GSATTSGGAITYY130 pKa = 7.49TPSAGFTGTDD140 pKa = 2.97SFTYY144 pKa = 10.03TIGDD148 pKa = 4.06SFSQTATATVTVTVNPPGLNAVDD171 pKa = 4.9DD172 pKa = 4.51AATTQARR179 pKa = 11.84TPVTIPVLTNDD190 pKa = 3.16VGAGLTITQVGAPAHH205 pKa = 5.95GSAVASAGGAITYY218 pKa = 7.94TPAPNFTGADD228 pKa = 3.19AFTYY232 pKa = 9.68TITDD236 pKa = 3.8GFSADD241 pKa = 3.43SATVRR246 pKa = 11.84VTVIAPALDD255 pKa = 3.95AVDD258 pKa = 5.31DD259 pKa = 4.33AATTQSRR266 pKa = 11.84IPVTIQNTGHH276 pKa = 7.21DD277 pKa = 4.01PGAGQQ282 pKa = 4.02

Molecular weight: 27.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A011PG62|A0A011PG62_9PROT Ethanolamine utilization protein EutJ OS=Candidatus Accumulibacter sp. BA-94 OX=1454005 GN=AW12_02141 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.6RR14 pKa = 11.84THH16 pKa = 5.73GFLVRR21 pKa = 11.84MSTRR25 pKa = 11.84GGRR28 pKa = 11.84GVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.62GRR39 pKa = 11.84HH40 pKa = 4.8RR41 pKa = 11.84LGVV44 pKa = 3.31

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3127

0

3127

836249

29

1657

267.4

29.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.241 ± 0.066

1.209 ± 0.019

5.332 ± 0.028

5.583 ± 0.043

3.494 ± 0.034

8.193 ± 0.043

2.351 ± 0.027

4.612 ± 0.032

2.776 ± 0.039

11.152 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.258 ± 0.019

2.434 ± 0.024

5.082 ± 0.026

3.866 ± 0.03

8.195 ± 0.054

5.48 ± 0.033

4.651 ± 0.033

7.437 ± 0.043

1.346 ± 0.022

2.175 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski