Amino acid dipepetide frequency for Candidatus Accumulibacter sp. BA-94

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.545AlaAla: 17.545 ± 0.203	1.625AlaCys: 1.625 ± 0.053	6.719AlaAsp: 6.719 ± 0.098	7.114AlaGlu: 7.114 ± 0.098	3.687AlaPhe: 3.687 ± 0.076	11.149AlaGly: 11.149 ± 0.138	2.554AlaHis: 2.554 ± 0.06	5.589AlaIle: 5.589 ± 0.096	3.067AlaLys: 3.067 ± 0.076	13.282AlaLeu: 13.282 ± 0.148	3.102AlaMet: 3.102 ± 0.073	2.811AlaAsn: 2.811 ± 0.06	4.874AlaPro: 4.874 ± 0.088	4.362AlaGln: 4.362 ± 0.078	9.812AlaArg: 9.812 ± 0.137	6.471AlaSer: 6.471 ± 0.091	5.837AlaThr: 5.837 ± 0.078	8.8AlaVal: 8.8 ± 0.115	1.581AlaTrp: 1.581 ± 0.048	2.406AlaTyr: 2.406 ± 0.047	0.025AlaXaa: 0.025 ± 0.006
Cys	1.412CysAla: 1.412 ± 0.044	0.248CysCys: 0.248 ± 0.02	0.624CysAsp: 0.624 ± 0.03	0.631CysGlu: 0.631 ± 0.029	0.36CysPhe: 0.36 ± 0.022	1.207CysGly: 1.207 ± 0.041	0.372CysHis: 0.372 ± 0.025	0.417CysIle: 0.417 ± 0.022	0.256CysLys: 0.256 ± 0.019	1.202CysLeu: 1.202 ± 0.04	0.216CysMet: 0.216 ± 0.018	0.261CysAsn: 0.261 ± 0.021	0.691CysPro: 0.691 ± 0.032	0.379CysGln: 0.379 ± 0.021	1.277CysArg: 1.277 ± 0.046	0.867CysSer: 0.867 ± 0.034	0.489CysThr: 0.489 ± 0.025	0.729CysVal: 0.729 ± 0.028	0.208CysTrp: 0.208 ± 0.015	0.237CysTyr: 0.237 ± 0.016	0.002CysXaa: 0.002 ± 0.001
Asp	6.219AspAla: 6.219 ± 0.094	0.601AspCys: 0.601 ± 0.026	3.198AspAsp: 3.198 ± 0.081	3.648AspGlu: 3.648 ± 0.075	2.187AspPhe: 2.187 ± 0.05	4.747AspGly: 4.747 ± 0.084	1.274AspHis: 1.274 ± 0.041	2.258AspIle: 2.258 ± 0.054	1.287AspLys: 1.287 ± 0.05	5.761AspLeu: 5.761 ± 0.073	0.918AspMet: 0.918 ± 0.033	1.226AspAsn: 1.226 ± 0.037	2.923AspPro: 2.923 ± 0.059	1.873AspGln: 1.873 ± 0.048	4.334AspArg: 4.334 ± 0.073	2.676AspSer: 2.676 ± 0.058	2.095AspThr: 2.095 ± 0.052	3.682AspVal: 3.682 ± 0.07	0.926AspTrp: 0.926 ± 0.033	1.473AspTyr: 1.473 ± 0.05	0.011AspXaa: 0.011 ± 0.004
Glu	6.584GluAla: 6.584 ± 0.098	0.497GluCys: 0.497 ± 0.026	2.309GluAsp: 2.309 ± 0.059	3.009GluGlu: 3.009 ± 0.066	1.913GluPhe: 1.913 ± 0.047	3.438GluGly: 3.438 ± 0.07	1.498GluHis: 1.498 ± 0.051	3.236GluIle: 3.236 ± 0.073	1.979GluLys: 1.979 ± 0.056	6.474GluLeu: 6.474 ± 0.1	1.464GluMet: 1.464 ± 0.037	1.394GluAsn: 1.394 ± 0.042	2.297GluPro: 2.297 ± 0.049	2.723GluGln: 2.723 ± 0.064	5.198GluArg: 5.198 ± 0.079	2.738GluSer: 2.738 ± 0.06	2.686GluThr: 2.686 ± 0.049	4.428GluVal: 4.428 ± 0.088	0.752GluTrp: 0.752 ± 0.03	1.203GluTyr: 1.203 ± 0.038	0.011GluXaa: 0.011 ± 0.003
Phe	4.512PheAla: 4.512 ± 0.076	0.471PheCys: 0.471 ± 0.026	2.325PheAsp: 2.325 ± 0.049	1.898PheGlu: 1.898 ± 0.043	1.464PhePhe: 1.464 ± 0.046	3.263PheGly: 3.263 ± 0.067	0.781PheHis: 0.781 ± 0.033	1.551PheIle: 1.551 ± 0.049	0.845PheLys: 0.845 ± 0.031	3.44PheLeu: 3.44 ± 0.079	0.668PheMet: 0.668 ± 0.033	1.008PheAsn: 1.008 ± 0.033	1.6PhePro: 1.6 ± 0.043	0.969PheGln: 0.969 ± 0.034	2.358PheArg: 2.358 ± 0.059	2.192PheSer: 2.192 ± 0.052	1.526PheThr: 1.526 ± 0.044	2.741PheVal: 2.741 ± 0.069	0.486PheTrp: 0.486 ± 0.028	0.839PheTyr: 0.839 ± 0.028	0.002PheXaa: 0.002 ± 0.002
Gly	8.151GlyAla: 8.151 ± 0.103	1.226GlyCys: 1.226 ± 0.043	4.556GlyAsp: 4.556 ± 0.072	5.234GlyGlu: 5.234 ± 0.085	3.164GlyPhe: 3.164 ± 0.061	6.742GlyGly: 6.742 ± 0.095	1.955GlyHis: 1.955 ± 0.047	4.209GlyIle: 4.209 ± 0.072	3.201GlyLys: 3.201 ± 0.075	8.38GlyLeu: 8.38 ± 0.11	2.204GlyMet: 2.204 ± 0.054	2.17GlyAsn: 2.17 ± 0.051	2.616GlyPro: 2.616 ± 0.068	3.251GlyGln: 3.251 ± 0.067	6.742GlyArg: 6.742 ± 0.094	4.934GlySer: 4.934 ± 0.083	3.714GlyThr: 3.714 ± 0.073	6.235GlyVal: 6.235 ± 0.097	1.241GlyTrp: 1.241 ± 0.038	1.972GlyTyr: 1.972 ± 0.055	0.033GlyXaa: 0.033 ± 0.006
His	2.667HisAla: 2.667 ± 0.055	0.374HisCys: 0.374 ± 0.023	1.324HisAsp: 1.324 ± 0.046	1.235HisGlu: 1.235 ± 0.04	1.036HisPhe: 1.036 ± 0.038	2.105HisGly: 2.105 ± 0.054	0.761HisHis: 0.761 ± 0.03	0.867HisIle: 0.867 ± 0.033	0.466HisLys: 0.466 ± 0.025	2.731HisLeu: 2.731 ± 0.059	0.374HisMet: 0.374 ± 0.02	0.481HisAsn: 0.481 ± 0.026	1.488HisPro: 1.488 ± 0.04	0.932HisGln: 0.932 ± 0.039	2.107HisArg: 2.107 ± 0.064	1.154HisSer: 1.154 ± 0.039	0.842HisThr: 0.842 ± 0.032	1.551HisVal: 1.551 ± 0.047	0.342HisTrp: 0.342 ± 0.018	0.674HisTyr: 0.674 ± 0.028	0.005HisXaa: 0.005 ± 0.002
Ile	6.127IleAla: 6.127 ± 0.09	0.53IleCys: 0.53 ± 0.025	3.333IleAsp: 3.333 ± 0.072	3.3IleGlu: 3.3 ± 0.061	1.396IlePhe: 1.396 ± 0.042	4.45IleGly: 4.45 ± 0.083	0.958IleHis: 0.958 ± 0.036	1.873IleIle: 1.873 ± 0.054	1.351IleLys: 1.351 ± 0.047	4.09IleLeu: 4.09 ± 0.08	0.796IleMet: 0.796 ± 0.034	1.448IleAsn: 1.448 ± 0.043	2.041IlePro: 2.041 ± 0.055	1.171IleGln: 1.171 ± 0.04	3.231IleArg: 3.231 ± 0.059	2.528IleSer: 2.528 ± 0.057	2.249IleThr: 2.249 ± 0.064	3.824IleVal: 3.824 ± 0.072	0.444IleTrp: 0.444 ± 0.024	0.976IleTyr: 0.976 ± 0.034	0.005IleXaa: 0.005 ± 0.002
Lys	3.254LysAla: 3.254 ± 0.087	0.199LysCys: 0.199 ± 0.015	1.33LysAsp: 1.33 ± 0.05	1.519LysGlu: 1.519 ± 0.05	0.744LysPhe: 0.744 ± 0.027	2.129LysGly: 2.129 ± 0.063	0.57LysHis: 0.57 ± 0.028	1.319LysIle: 1.319 ± 0.042	1.075LysLys: 1.075 ± 0.049	3.097LysLeu: 3.097 ± 0.071	0.712LysMet: 0.712 ± 0.031	0.792LysAsn: 0.792 ± 0.039	1.643LysPro: 1.643 ± 0.05	1.058LysGln: 1.058 ± 0.039	2.132LysArg: 2.132 ± 0.056	1.521LysSer: 1.521 ± 0.045	1.605LysThr: 1.605 ± 0.043	2.115LysVal: 2.115 ± 0.061	0.32LysTrp: 0.32 ± 0.022	0.622LysTyr: 0.622 ± 0.026	0.01LysXaa: 0.01 ± 0.003
Leu	15.724LeuAla: 15.724 ± 0.166	1.242LeuCys: 1.242 ± 0.038	6.032LeuAsp: 6.032 ± 0.085	5.477LeuGlu: 5.477 ± 0.093	3.638LeuPhe: 3.638 ± 0.071	8.347LeuGly: 8.347 ± 0.094	2.478LeuHis: 2.478 ± 0.062	4.851LeuIle: 4.851 ± 0.083	2.903LeuLys: 2.903 ± 0.072	13.535LeuLeu: 13.535 ± 0.209	2.15LeuMet: 2.15 ± 0.048	2.4LeuAsn: 2.4 ± 0.056	6.771LeuPro: 6.771 ± 0.105	4.384LeuGln: 4.384 ± 0.069	9.009LeuArg: 9.009 ± 0.133	6.096LeuSer: 6.096 ± 0.089	5.225LeuThr: 5.225 ± 0.089	7.774LeuVal: 7.774 ± 0.121	1.323LeuTrp: 1.323 ± 0.047	2.129LeuTyr: 2.129 ± 0.051	0.035LeuXaa: 0.035 ± 0.006
Met	2.73MetAla: 2.73 ± 0.06	0.164MetCys: 0.164 ± 0.015	0.982MetAsp: 0.982 ± 0.035	0.909MetGlu: 0.909 ± 0.031	0.611MetPhe: 0.611 ± 0.027	1.513MetGly: 1.513 ± 0.044	0.557MetHis: 0.557 ± 0.03	1.202MetIle: 1.202 ± 0.035	0.918MetLys: 0.918 ± 0.034	2.575MetLeu: 2.575 ± 0.053	0.538MetMet: 0.538 ± 0.026	0.75MetAsn: 0.75 ± 0.029	1.437MetPro: 1.437 ± 0.038	0.963MetGln: 0.963 ± 0.032	1.801MetArg: 1.801 ± 0.045	1.491MetSer: 1.491 ± 0.04	1.351MetThr: 1.351 ± 0.036	1.558MetVal: 1.558 ± 0.048	0.209MetTrp: 0.209 ± 0.015	0.313MetTyr: 0.313 ± 0.019	0.006MetXaa: 0.006 ± 0.003
Asn	2.676AsnAla: 2.676 ± 0.056	0.319AsnCys: 0.319 ± 0.019	1.281AsnAsp: 1.281 ± 0.039	1.227AsnGlu: 1.227 ± 0.041	0.871AsnPhe: 0.871 ± 0.035	2.082AsnGly: 2.082 ± 0.049	0.568AsnHis: 0.568 ± 0.024	1.159AsnIle: 1.159 ± 0.043	0.667AsnLys: 0.667 ± 0.031	2.7AsnLeu: 2.7 ± 0.053	0.517AsnMet: 0.517 ± 0.028	0.645AsnAsn: 0.645 ± 0.03	1.755AsnPro: 1.755 ± 0.047	0.847AsnGln: 0.847 ± 0.034	1.974AsnArg: 1.974 ± 0.05	1.24AsnSer: 1.24 ± 0.043	1.089AsnThr: 1.089 ± 0.039	1.748AsnVal: 1.748 ± 0.042	0.378AsnTrp: 0.378 ± 0.023	0.598AsnTyr: 0.598 ± 0.026	0.004AsnXaa: 0.004 ± 0.002
Pro	7.027ProAla: 7.027 ± 0.112	0.529ProCys: 0.529 ± 0.028	2.924ProAsp: 2.924 ± 0.062	3.169ProGlu: 3.169 ± 0.062	1.7ProPhe: 1.7 ± 0.049	4.563ProGly: 4.563 ± 0.076	1.013ProHis: 1.013 ± 0.041	1.942ProIle: 1.942 ± 0.047	1.221ProLys: 1.221 ± 0.04	5.58ProLeu: 5.58 ± 0.083	1.1ProMet: 1.1 ± 0.038	1.081ProAsn: 1.081 ± 0.037	2.988ProPro: 2.988 ± 0.071	2.015ProGln: 2.015 ± 0.05	3.344ProArg: 3.344 ± 0.068	2.399ProSer: 2.399 ± 0.056	2.377ProThr: 2.377 ± 0.054	4.022ProVal: 4.022 ± 0.062	0.735ProTrp: 0.735 ± 0.032	1.076ProTyr: 1.076 ± 0.036	0.019ProXaa: 0.019 ± 0.005
Gln	4.875GlnAla: 4.875 ± 0.091	0.323GlnCys: 0.323 ± 0.019	1.47GlnAsp: 1.47 ± 0.051	1.903GlnGlu: 1.903 ± 0.053	1.156GlnPhe: 1.156 ± 0.037	2.652GlnGly: 2.652 ± 0.051	0.991GlnHis: 0.991 ± 0.038	1.855GlnIle: 1.855 ± 0.047	1.119GlnLys: 1.119 ± 0.042	4.392GlnLeu: 4.392 ± 0.082	0.979GlnMet: 0.979 ± 0.038	0.756GlnAsn: 0.756 ± 0.033	2.323GlnPro: 2.323 ± 0.057	2.108GlnGln: 2.108 ± 0.061	3.939GlnArg: 3.939 ± 0.077	1.881GlnSer: 1.881 ± 0.048	1.801GlnThr: 1.801 ± 0.049	2.937GlnVal: 2.937 ± 0.068	0.47GlnTrp: 0.47 ± 0.024	0.715GlnTyr: 0.715 ± 0.028	0.018GlnXaa: 0.018 ± 0.004
Arg	8.084ArgAla: 8.084 ± 0.114	1.18ArgCys: 1.18 ± 0.044	3.896ArgAsp: 3.896 ± 0.075	4.584ArgGlu: 4.584 ± 0.081	3.047ArgPhe: 3.047 ± 0.064	5.153ArgGly: 5.153 ± 0.083	2.535ArgHis: 2.535 ± 0.066	4.0ArgIle: 4.0 ± 0.069	2.228ArgLys: 2.228 ± 0.049	10.042ArgLeu: 10.042 ± 0.13	2.078ArgMet: 2.078 ± 0.049	2.042ArgAsn: 2.042 ± 0.048	4.017ArgPro: 4.017 ± 0.07	4.269ArgGln: 4.269 ± 0.081	7.975ArgArg: 7.975 ± 0.149	4.634ArgSer: 4.634 ± 0.091	3.273ArgThr: 3.273 ± 0.067	5.665ArgVal: 5.665 ± 0.086	1.211ArgTrp: 1.211 ± 0.043	2.008ArgTyr: 2.008 ± 0.053	0.031ArgXaa: 0.031 ± 0.006
Ser	6.1SerAla: 6.1 ± 0.091	0.685SerCys: 0.685 ± 0.031	2.658SerAsp: 2.658 ± 0.06	2.981SerGlu: 2.981 ± 0.061	2.185SerPhe: 2.185 ± 0.055	5.506SerGly: 5.506 ± 0.086	1.124SerHis: 1.124 ± 0.04	2.515SerIle: 2.515 ± 0.055	1.374SerLys: 1.374 ± 0.046	6.074SerLeu: 6.074 ± 0.097	1.317SerMet: 1.317 ± 0.036	1.256SerAsn: 1.256 ± 0.041	3.085SerPro: 3.085 ± 0.063	1.778SerGln: 1.778 ± 0.045	4.374SerArg: 4.374 ± 0.083	3.378SerSer: 3.378 ± 0.072	2.594SerThr: 2.594 ± 0.063	3.744SerVal: 3.744 ± 0.065	0.764SerTrp: 0.764 ± 0.03	1.3SerTyr: 1.3 ± 0.043	0.012SerXaa: 0.012 ± 0.004
Thr	5.699ThrAla: 5.699 ± 0.086	0.48ThrCys: 0.48 ± 0.027	2.225ThrAsp: 2.225 ± 0.051	1.992ThrGlu: 1.992 ± 0.049	1.483ThrPhe: 1.483 ± 0.042	4.267ThrGly: 4.267 ± 0.076	1.004ThrHis: 1.004 ± 0.039	2.334ThrIle: 2.334 ± 0.058	0.899ThrLys: 0.899 ± 0.034	5.573ThrLeu: 5.573 ± 0.088	1.033ThrMet: 1.033 ± 0.035	0.993ThrAsn: 0.993 ± 0.033	2.99ThrPro: 2.99 ± 0.064	1.63ThrGln: 1.63 ± 0.047	3.385ThrArg: 3.385 ± 0.059	2.455ThrSer: 2.455 ± 0.059	2.6ThrThr: 2.6 ± 0.06	3.913ThrVal: 3.913 ± 0.078	0.6ThrTrp: 0.6 ± 0.029	0.934ThrTyr: 0.934 ± 0.037	0.018ThrXaa: 0.018 ± 0.005
Val	9.662ValAla: 9.662 ± 0.123	0.914ValCys: 0.914 ± 0.035	4.44ValAsp: 4.44 ± 0.084	4.373ValGlu: 4.373 ± 0.083	2.863ValPhe: 2.863 ± 0.06	5.909ValGly: 5.909 ± 0.102	1.622ValHis: 1.622 ± 0.045	3.564ValIle: 3.564 ± 0.065	1.953ValLys: 1.953 ± 0.058	7.931ValLeu: 7.931 ± 0.113	1.671ValMet: 1.671 ± 0.051	1.859ValAsn: 1.859 ± 0.04	3.382ValPro: 3.382 ± 0.073	2.174ValGln: 2.174 ± 0.05	5.498ValArg: 5.498 ± 0.079	4.201ValSer: 4.201 ± 0.08	3.561ValThr: 3.561 ± 0.075	6.499ValVal: 6.499 ± 0.116	0.871ValTrp: 0.871 ± 0.032	1.407ValTyr: 1.407 ± 0.047	0.022ValXaa: 0.022 ± 0.005
Trp	1.049TrpAla: 1.049 ± 0.038	0.176TrpCys: 0.176 ± 0.015	0.537TrpAsp: 0.537 ± 0.025	0.58TrpGlu: 0.58 ± 0.026	0.538TrpPhe: 0.538 ± 0.028	0.784TrpGly: 0.784 ± 0.033	0.332TrpHis: 0.332 ± 0.02	0.628TrpIle: 0.628 ± 0.028	0.405TrpLys: 0.405 ± 0.024	2.096TrpLeu: 2.096 ± 0.06	0.343TrpMet: 0.343 ± 0.021	0.381TrpAsn: 0.381 ± 0.023	0.721TrpPro: 0.721 ± 0.031	0.883TrpGln: 0.883 ± 0.037	1.357TrpArg: 1.357 ± 0.04	0.732TrpSer: 0.732 ± 0.029	0.575TrpThr: 0.575 ± 0.029	0.823TrpVal: 0.823 ± 0.029	0.242TrpTrp: 0.242 ± 0.019	0.276TrpTyr: 0.276 ± 0.021	0.002TrpXaa: 0.002 ± 0.002
Tyr	2.285TyrAla: 2.285 ± 0.051	0.293TyrCys: 0.293 ± 0.022	1.142TyrAsp: 1.142 ± 0.043	1.037TyrGlu: 1.037 ± 0.034	0.892TyrPhe: 0.892 ± 0.037	1.905TyrGly: 1.905 ± 0.051	0.558TyrHis: 0.558 ± 0.028	0.747TyrIle: 0.747 ± 0.031	0.543TyrLys: 0.543 ± 0.03	2.551TyrLeu: 2.551 ± 0.058	0.39TyrMet: 0.39 ± 0.021	0.587TyrAsn: 0.587 ± 0.028	1.158TyrPro: 1.158 ± 0.037	0.887TyrGln: 0.887 ± 0.035	2.066TyrArg: 2.066 ± 0.058	1.191TyrSer: 1.191 ± 0.041	1.007TyrThr: 1.007 ± 0.035	1.569TyrVal: 1.569 ± 0.046	0.349TyrTrp: 0.349 ± 0.021	0.594TyrTyr: 0.594 ± 0.033	0.002TyrXaa: 0.002 ± 0.002
Xaa	0.03XaaAla: 0.03 ± 0.006	0.011XaaCys: 0.011 ± 0.003	0.013XaaAsp: 0.013 ± 0.004	0.013XaaGlu: 0.013 ± 0.004	0.006XaaPhe: 0.006 ± 0.003	0.024XaaGly: 0.024 ± 0.005	0.008XaaHis: 0.008 ± 0.003	0.005XaaIle: 0.005 ± 0.002	0.005XaaLys: 0.005 ± 0.002	0.016XaaLeu: 0.016 ± 0.004	0.006XaaMet: 0.006 ± 0.003	0.004XaaAsn: 0.004 ± 0.002	0.024XaaPro: 0.024 ± 0.005	0.01XaaGln: 0.01 ± 0.004	0.037XaaArg: 0.037 ± 0.006	0.024XaaSer: 0.024 ± 0.005	0.011XaaThr: 0.011 ± 0.004	0.017XaaVal: 0.017 ± 0.004	0.01XaaTrp: 0.01 ± 0.003	0.001XaaTyr: 0.001 ± 0.001	1.025XaaXaa: 1.025 ± 0.088

Statistics based on 3127 proteins (836249 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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