Roseomonas deserti
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5405 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V2GXD0|A0A1V2GXD0_9PROT GNAT family N-acetyltransferase OS=Roseomonas deserti OX=1817963 GN=BKE38_23685 PE=4 SV=1
MM1 pKa = 7.98 CIRR4 pKa = 11.84 EE5 pKa = 4.03 RR6 pKa = 11.84 RR7 pKa = 11.84 GGAGDD12 pKa = 4.98 DD13 pKa = 3.73 ILVGGPGYY21 pKa = 10.71 DD22 pKa = 3.37 ILDD25 pKa = 3.86 GGAGIDD31 pKa = 3.78 HH32 pKa = 6.15 YY33 pKa = 11.2 RR34 pKa = 11.84 ILAPNDD40 pKa = 4.09 GYY42 pKa = 10.61 DD43 pKa = 3.26 TLAYY47 pKa = 10.61 VPGEE51 pKa = 3.96 DD52 pKa = 4.35 VIEE55 pKa = 4.17 ISAAAFGGGLVAGMDD70 pKa = 4.42 LGASGYY76 pKa = 8.5 YY77 pKa = 10.44 LPGATAAASAHH88 pKa = 5.23 GQFLSVGGVLSYY100 pKa = 10.64 DD101 pKa = 3.66 ANGIAPGGLILVARR115 pKa = 11.84 TGVPVLFDD123 pKa = 4.24 DD124 pKa = 5.35 LVIIAA129 pKa = 5.03
Molecular weight: 12.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.803
IPC2_protein 3.948
IPC_protein 3.923
Toseland 3.694
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.91
Rodwell 3.745
Grimsley 3.605
Solomon 3.923
Lehninger 3.872
Nozaki 4.062
DTASelect 4.342
Thurlkill 3.77
EMBOSS 3.923
Sillero 4.05
Patrickios 0.604
IPC_peptide 3.91
IPC2_peptide 4.012
IPC2.peptide.svr19 3.916
Protein with the highest isoelectric point:
>tr|A0A1V2H462|A0A1V2H462_9PROT Uncharacterized protein OS=Roseomonas deserti OX=1817963 GN=BKE38_08620 PE=4 SV=1
MM1 pKa = 7.51 LRR3 pKa = 11.84 PLVLRR8 pKa = 11.84 PLVLRR13 pKa = 11.84 PLVLRR18 pKa = 11.84 PLVLRR23 pKa = 11.84 PLVLRR28 pKa = 11.84 PLVLRR33 pKa = 11.84 PLVLRR38 pKa = 11.84 PLVLRR43 pKa = 11.84 RR44 pKa = 11.84 LVLRR48 pKa = 11.84 RR49 pKa = 11.84 LVLRR53 pKa = 11.84 RR54 pKa = 11.84 LVLRR58 pKa = 11.84 PLVLRR63 pKa = 11.84 PLVLRR68 pKa = 11.84 RR69 pKa = 11.84 PVLRR73 pKa = 11.84 RR74 pKa = 11.84 VVLRR78 pKa = 11.84 RR79 pKa = 11.84 LVLRR83 pKa = 11.84 PLVLRR88 pKa = 11.84 PLVLRR93 pKa = 11.84 RR94 pKa = 11.84 VVLRR98 pKa = 11.84 RR99 pKa = 11.84 LVLRR103 pKa = 11.84 RR104 pKa = 11.84 LVLRR108 pKa = 11.84 RR109 pKa = 11.84 LVLRR113 pKa = 11.84 PLVLRR118 pKa = 11.84 PLAVSSVKK126 pKa = 10.48 YY127 pKa = 9.44 MPSIPHH133 pKa = 6.03 PRR135 pKa = 11.84 LRR137 pKa = 11.84 NQLQFSATTFWKK149 pKa = 10.62 AFAAARR155 pKa = 3.87
Molecular weight: 18.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.503
IPC2_protein 11.579
IPC_protein 13.086
Toseland 13.247
ProMoST 13.744
Dawson 13.247
Bjellqvist 13.247
Wikipedia 13.729
Rodwell 12.764
Grimsley 13.29
Solomon 13.744
Lehninger 13.642
Nozaki 13.247
DTASelect 13.247
Thurlkill 13.247
EMBOSS 13.744
Sillero 13.247
Patrickios 12.486
IPC_peptide 13.744
IPC2_peptide 12.735
IPC2.peptide.svr19 9.388
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5405
0
5405
1725895
26
2417
319.3
34.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
15.118 ± 0.05
0.788 ± 0.01
5.013 ± 0.028
5.397 ± 0.029
3.317 ± 0.02
9.351 ± 0.036
1.929 ± 0.016
4.059 ± 0.022
2.022 ± 0.023
11.656 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.341 ± 0.013
1.966 ± 0.018
6.091 ± 0.034
3.386 ± 0.019
8.017 ± 0.034
4.63 ± 0.023
4.753 ± 0.022
6.974 ± 0.025
1.435 ± 0.014
1.759 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here