Circo-like virus-Brazil hs2

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses; Circo-like virus-Brazil

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U3LZQ2|U3LZQ2_9VIRU ATP-dependent helicase Rep OS=Circo-like virus-Brazil hs2 OX=1346816 PE=3 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84KK3 pKa = 9.85YY4 pKa = 10.67KK5 pKa = 8.88ITKK8 pKa = 10.09NGLTDD13 pKa = 3.5YY14 pKa = 9.4STQKK18 pKa = 10.1PVSTIYY24 pKa = 10.33VGKK27 pKa = 10.22EE28 pKa = 3.12KK29 pKa = 10.57DD30 pKa = 3.48VQIQEE35 pKa = 3.99DD36 pKa = 4.92YY37 pKa = 10.51IYY39 pKa = 11.04KK40 pKa = 8.94DD41 pKa = 2.89TWSLQEE47 pKa = 4.81KK48 pKa = 9.71KK49 pKa = 10.85DD50 pKa = 4.19LMEE53 pKa = 4.32QKK55 pKa = 10.36KK56 pKa = 10.55DD57 pKa = 3.33WIVPEE62 pKa = 4.82FIWKK66 pKa = 9.7EE67 pKa = 3.58EE68 pKa = 3.75WVRR71 pKa = 11.84TGKK74 pKa = 9.99RR75 pKa = 11.84WNTVKK80 pKa = 10.36KK81 pKa = 10.52RR82 pKa = 11.84SLEE85 pKa = 4.07MVNSLNLVNMMIEE98 pKa = 4.01EE99 pKa = 4.06ARR101 pKa = 11.84RR102 pKa = 11.84NVWKK106 pKa = 10.45KK107 pKa = 10.42SKK109 pKa = 10.59KK110 pKa = 8.65EE111 pKa = 4.07SEE113 pKa = 4.48KK114 pKa = 9.7EE115 pKa = 4.34TQNLTSQKK123 pKa = 8.31NTLGFGVDD131 pKa = 3.6TTKK134 pKa = 10.94HH135 pKa = 5.26LVNTEE140 pKa = 2.99QWYY143 pKa = 10.05NLEE146 pKa = 3.92EE147 pKa = 4.1TLRR150 pKa = 11.84PLSEE154 pKa = 5.4FIGEE158 pKa = 4.12DD159 pKa = 3.23QEE161 pKa = 5.37LVNQEE166 pKa = 4.24EE167 pKa = 5.02LPTNVDD173 pKa = 2.37QMYY176 pKa = 10.57IEE178 pKa = 4.42NRR180 pKa = 11.84WEE182 pKa = 3.98NGGMDD187 pKa = 3.43ITEE190 pKa = 4.65HH191 pKa = 5.72LTSLSMTSMDD201 pKa = 4.7GSDD204 pKa = 3.09LMNYY208 pKa = 9.47FDD210 pKa = 6.14ALIGTNTVFQQ220 pKa = 4.07

Molecular weight: 26.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U3LYW2|U3LYW2_9VIRU Uncharacterized protein OS=Circo-like virus-Brazil hs2 OX=1346816 PE=4 SV=1
MM1 pKa = 7.83VMRR4 pKa = 11.84LCGWVILDD12 pKa = 3.97QPPKK16 pKa = 9.51RR17 pKa = 11.84TPDD20 pKa = 3.56LEE22 pKa = 4.08IKK24 pKa = 9.81TNLEE28 pKa = 3.73SFLIMSWWDD37 pKa = 4.22DD38 pKa = 3.37IPDD41 pKa = 3.41EE42 pKa = 4.18SFYY45 pKa = 11.45YY46 pKa = 10.08IDD48 pKa = 3.54TPVFKK53 pKa = 10.38SAYY56 pKa = 6.5EE57 pKa = 4.0TLSDD61 pKa = 3.8RR62 pKa = 11.84EE63 pKa = 4.29AIKK66 pKa = 10.45ISNLVEE72 pKa = 3.91LRR74 pKa = 11.84KK75 pKa = 10.03KK76 pKa = 9.09EE77 pKa = 3.97LKK79 pKa = 10.41KK80 pKa = 10.24LNKK83 pKa = 9.96YY84 pKa = 9.08IASLYY89 pKa = 8.19VYY91 pKa = 9.96KK92 pKa = 10.96YY93 pKa = 10.61KK94 pKa = 10.83LDD96 pKa = 3.88GRR98 pKa = 11.84DD99 pKa = 2.86TIYY102 pKa = 11.1NVRR105 pKa = 11.84NKK107 pKa = 9.33ATEE110 pKa = 3.72VRR112 pKa = 11.84NTIEE116 pKa = 4.97KK117 pKa = 9.69IASNAKK123 pKa = 9.81VKK125 pKa = 9.85WSSKK129 pKa = 7.62GWKK132 pKa = 9.42GWDD135 pKa = 3.17IEE137 pKa = 4.25RR138 pKa = 11.84KK139 pKa = 9.6AFKK142 pKa = 10.17KK143 pKa = 10.39FKK145 pKa = 10.45KK146 pKa = 10.06NYY148 pKa = 7.99PNSEE152 pKa = 3.67TDD154 pKa = 3.05YY155 pKa = 10.96TIIVDD160 pKa = 4.2LMAHH164 pKa = 5.87YY165 pKa = 9.25FIKK168 pKa = 10.21MGEE171 pKa = 4.06NLKK174 pKa = 10.82DD175 pKa = 4.08LIDD178 pKa = 4.04RR179 pKa = 11.84RR180 pKa = 11.84LKK182 pKa = 7.2TTSRR186 pKa = 11.84KK187 pKa = 8.91KK188 pKa = 9.4YY189 pKa = 9.34RR190 pKa = 11.84WVATYY195 pKa = 9.63TPTEE199 pKa = 4.01KK200 pKa = 10.57KK201 pKa = 10.32IFMPYY206 pKa = 10.21

Molecular weight: 24.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

959

206

287

239.8

28.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.815 ± 0.58

1.043 ± 0.6

6.048 ± 0.476

8.551 ± 1.157

4.38 ± 0.727

5.631 ± 1.052

1.147 ± 0.189

6.465 ± 0.567

9.593 ± 1.503

7.091 ± 0.452

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.92 ± 0.526

5.631 ± 0.63

3.128 ± 0.457

3.233 ± 0.841

6.674 ± 1.356

5.109 ± 0.325

6.674 ± 1.15

5.318 ± 0.367

3.024 ± 0.291

5.527 ± 0.555
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski