Streptococcus satellite phage Javan725

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZUS5|A0A4D5ZUS5_9VIRU Putative transcriptional regulator OS=Streptococcus satellite phage Javan725 OX=2558819 GN=JavanS725_0002 PE=4 SV=1
MM1 pKa = 6.88TLKK4 pKa = 10.04TFSDD8 pKa = 3.49KK9 pKa = 11.46AKK11 pKa = 9.54TFTFTYY17 pKa = 9.87EE18 pKa = 4.22FKK20 pKa = 11.13DD21 pKa = 3.28LDD23 pKa = 3.75TAMVAGHH30 pKa = 7.15ALLGYY35 pKa = 7.49MTGTYY40 pKa = 8.01EE41 pKa = 4.13VPSISITHH49 pKa = 6.69KK50 pKa = 11.13DD51 pKa = 2.96KK52 pKa = 10.45GTLVAEE58 pKa = 4.26YY59 pKa = 11.19VEE61 pKa = 4.12DD62 pKa = 3.62HH63 pKa = 6.67KK64 pKa = 11.65LNKK67 pKa = 8.14TFKK70 pKa = 10.29RR71 pKa = 11.84ICDD74 pKa = 3.7SFKK77 pKa = 10.87DD78 pKa = 4.26YY79 pKa = 11.51YY80 pKa = 9.82NQPVDD85 pKa = 4.45DD86 pKa = 4.12EE87 pKa = 4.7AFEE90 pKa = 4.06EE91 pKa = 4.93RR92 pKa = 11.84YY93 pKa = 9.8KK94 pKa = 10.7RR95 pKa = 11.84EE96 pKa = 3.48RR97 pKa = 11.84VLQLKK102 pKa = 10.01EE103 pKa = 4.24SEE105 pKa = 4.3DD106 pKa = 3.88FEE108 pKa = 4.67NLLEE112 pKa = 4.39KK113 pKa = 9.9VTDD116 pKa = 3.92YY117 pKa = 11.2EE118 pKa = 5.77LEE120 pKa = 4.13LLDD123 pKa = 3.9YY124 pKa = 11.08ADD126 pKa = 5.31RR127 pKa = 11.84LLSDD131 pKa = 4.21KK132 pKa = 9.93PIPMDD137 pKa = 3.3SMTAFGTLEE146 pKa = 3.9MLGDD150 pKa = 3.83EE151 pKa = 5.38SISLLQKK158 pKa = 10.52LDD160 pKa = 3.32VEE162 pKa = 4.81GEE164 pKa = 4.33YY165 pKa = 10.49KK166 pKa = 10.72GLAGYY171 pKa = 7.7TEE173 pKa = 4.17HH174 pKa = 7.28LKK176 pKa = 10.96

Molecular weight: 20.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZTU5|A0A4D5ZTU5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan725 OX=2558819 GN=JavanS725_0007 PE=4 SV=1
MM1 pKa = 7.42ARR3 pKa = 11.84YY4 pKa = 9.68SIHH7 pKa = 6.5PADD10 pKa = 4.54NKK12 pKa = 10.47GHH14 pKa = 4.76YY15 pKa = 10.21HH16 pKa = 6.35NIKK19 pKa = 9.63FYY21 pKa = 10.59KK22 pKa = 9.39EE23 pKa = 3.65RR24 pKa = 11.84RR25 pKa = 11.84PTLDD29 pKa = 3.03QLKK32 pKa = 9.39EE33 pKa = 3.8QQRR36 pKa = 11.84LKK38 pKa = 10.73KK39 pKa = 10.24LKK41 pKa = 9.41KK42 pKa = 9.51KK43 pKa = 9.73RR44 pKa = 11.84RR45 pKa = 11.84KK46 pKa = 9.75GRR48 pKa = 3.39

Molecular weight: 5.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19

0

19

2970

43

492

156.3

18.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.589 ± 0.366

0.808 ± 0.199

5.421 ± 0.489

8.485 ± 0.69

4.68 ± 0.368

4.68 ± 0.372

1.818 ± 0.269

7.037 ± 0.536

9.428 ± 0.518

9.899 ± 0.559

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.626 ± 0.421

5.185 ± 0.517

3.098 ± 0.525

3.737 ± 0.359

5.657 ± 0.563

5.488 ± 0.319

6.195 ± 0.335

4.949 ± 0.531

0.842 ± 0.198

4.377 ± 0.302
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski