Amino acid dipepetide frequency for Paenibacillus sp. HB172198

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.015AlaAla: 10.015 ± 0.16
0.729AlaCys: 0.729 ± 0.027
4.015AlaAsp: 4.015 ± 0.057
6.098AlaGlu: 6.098 ± 0.092
3.182AlaPhe: 3.182 ± 0.056
7.356AlaGly: 7.356 ± 0.101
1.339AlaHis: 1.339 ± 0.037
4.4AlaIle: 4.4 ± 0.065
3.879AlaLys: 3.879 ± 0.059
8.442AlaLeu: 8.442 ± 0.095
2.439AlaMet: 2.439 ± 0.046
2.131AlaAsn: 2.131 ± 0.045
3.044AlaPro: 3.044 ± 0.064
2.949AlaGln: 2.949 ± 0.053
3.76AlaArg: 3.76 ± 0.063
5.21AlaSer: 5.21 ± 0.069
2.925AlaThr: 2.925 ± 0.051
6.795AlaVal: 6.795 ± 0.083
1.004AlaTrp: 1.004 ± 0.032
2.593AlaTyr: 2.593 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.524CysAla: 0.524 ± 0.023
0.122CysCys: 0.122 ± 0.01
0.392CysAsp: 0.392 ± 0.019
0.471CysGlu: 0.471 ± 0.025
0.288CysPhe: 0.288 ± 0.015
0.777CysGly: 0.777 ± 0.028
0.209CysHis: 0.209 ± 0.013
0.523CysIle: 0.523 ± 0.021
0.308CysLys: 0.308 ± 0.017
0.74CysLeu: 0.74 ± 0.024
0.223CysMet: 0.223 ± 0.012
0.239CysAsn: 0.239 ± 0.014
0.353CysPro: 0.353 ± 0.019
0.244CysGln: 0.244 ± 0.016
0.516CysArg: 0.516 ± 0.021
0.639CysSer: 0.639 ± 0.023
0.401CysThr: 0.401 ± 0.017
0.457CysVal: 0.457 ± 0.018
0.082CysTrp: 0.082 ± 0.008
0.274CysTyr: 0.274 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.872AspAla: 3.872 ± 0.058
0.388AspCys: 0.388 ± 0.019
2.28AspAsp: 2.28 ± 0.052
3.822AspGlu: 3.822 ± 0.054
2.06AspPhe: 2.06 ± 0.043
3.747AspGly: 3.747 ± 0.065
1.302AspHis: 1.302 ± 0.035
3.548AspIle: 3.548 ± 0.059
2.411AspLys: 2.411 ± 0.05
4.964AspLeu: 4.964 ± 0.061
1.549AspMet: 1.549 ± 0.033
1.583AspAsn: 1.583 ± 0.043
2.484AspPro: 2.484 ± 0.05
2.295AspGln: 2.295 ± 0.04
2.729AspArg: 2.729 ± 0.043
2.82AspSer: 2.82 ± 0.051
2.499AspThr: 2.499 ± 0.049
3.589AspVal: 3.589 ± 0.052
0.772AspTrp: 0.772 ± 0.026
2.002AspTyr: 2.002 ± 0.046
0.0AspXaa: 0.0 ± 0.0
Glu
6.592GluAla: 6.592 ± 0.086
0.429GluCys: 0.429 ± 0.023
3.616GluAsp: 3.616 ± 0.057
6.464GluGlu: 6.464 ± 0.103
2.103GluPhe: 2.103 ± 0.043
4.963GluGly: 4.963 ± 0.066
1.731GluHis: 1.731 ± 0.044
4.157GluIle: 4.157 ± 0.064
3.844GluLys: 3.844 ± 0.062
7.896GluLeu: 7.896 ± 0.098
2.147GluMet: 2.147 ± 0.05
2.29GluAsn: 2.29 ± 0.047
2.57GluPro: 2.57 ± 0.048
4.216GluGln: 4.216 ± 0.08
4.238GluArg: 4.238 ± 0.068
3.727GluSer: 3.727 ± 0.061
3.138GluThr: 3.138 ± 0.058
4.925GluVal: 4.925 ± 0.067
0.971GluTrp: 0.971 ± 0.027
2.006GluTyr: 2.006 ± 0.039
0.0GluXaa: 0.0 ± 0.0
Phe
2.853PheAla: 2.853 ± 0.05
0.341PheCys: 0.341 ± 0.017
2.147PheAsp: 2.147 ± 0.045
2.343PheGlu: 2.343 ± 0.039
1.611PhePhe: 1.611 ± 0.043
2.921PheGly: 2.921 ± 0.056
0.938PheHis: 0.938 ± 0.03
2.597PheIle: 2.597 ± 0.048
1.957PheLys: 1.957 ± 0.04
3.569PheLeu: 3.569 ± 0.065
1.207PheMet: 1.207 ± 0.029
1.436PheAsn: 1.436 ± 0.039
1.49PhePro: 1.49 ± 0.039
1.668PheGln: 1.668 ± 0.035
1.958PheArg: 1.958 ± 0.048
2.723PheSer: 2.723 ± 0.051
2.39PheThr: 2.39 ± 0.051
2.603PheVal: 2.603 ± 0.048
0.494PheTrp: 0.494 ± 0.022
1.398PheTyr: 1.398 ± 0.034
0.0PheXaa: 0.0 ± 0.0
Gly
5.692GlyAla: 5.692 ± 0.091
0.715GlyCys: 0.715 ± 0.024
3.58GlyAsp: 3.58 ± 0.053
4.846GlyGlu: 4.846 ± 0.061
3.127GlyPhe: 3.127 ± 0.058
5.681GlyGly: 5.681 ± 0.083
1.59GlyHis: 1.59 ± 0.038
5.32GlyIle: 5.32 ± 0.069
4.106GlyLys: 4.106 ± 0.056
7.258GlyLeu: 7.258 ± 0.088
2.571GlyMet: 2.571 ± 0.043
2.459GlyAsn: 2.459 ± 0.053
2.086GlyPro: 2.086 ± 0.044
2.934GlyGln: 2.934 ± 0.055
3.885GlyArg: 3.885 ± 0.063
5.174GlySer: 5.174 ± 0.076
4.27GlyThr: 4.27 ± 0.064
5.273GlyVal: 5.273 ± 0.075
1.061GlyTrp: 1.061 ± 0.033
2.928GlyTyr: 2.928 ± 0.054
0.0GlyXaa: 0.0 ± 0.0
His
1.716HisAla: 1.716 ± 0.04
0.241HisCys: 0.241 ± 0.015
1.058HisAsp: 1.058 ± 0.032
1.55HisGlu: 1.55 ± 0.038
1.043HisPhe: 1.043 ± 0.029
1.712HisGly: 1.712 ± 0.043
0.703HisHis: 0.703 ± 0.025
1.471HisIle: 1.471 ± 0.038
0.9HisLys: 0.9 ± 0.027
2.134HisLeu: 2.134 ± 0.051
0.631HisMet: 0.631 ± 0.023
0.673HisAsn: 0.673 ± 0.022
1.324HisPro: 1.324 ± 0.033
0.969HisGln: 0.969 ± 0.03
1.261HisArg: 1.261 ± 0.036
1.34HisSer: 1.34 ± 0.031
1.154HisThr: 1.154 ± 0.03
1.532HisVal: 1.532 ± 0.04
0.347HisTrp: 0.347 ± 0.017
0.915HisTyr: 0.915 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
5.069IleAla: 5.069 ± 0.075
0.559IleCys: 0.559 ± 0.022
3.156IleAsp: 3.156 ± 0.056
4.111IleGlu: 4.111 ± 0.06
2.111IlePhe: 2.111 ± 0.045
4.741IleGly: 4.741 ± 0.072
1.615IleHis: 1.615 ± 0.037
3.706IleIle: 3.706 ± 0.057
2.699IleLys: 2.699 ± 0.048
5.532IleLeu: 5.532 ± 0.066
1.641IleMet: 1.641 ± 0.038
2.147IleAsn: 2.147 ± 0.05
3.153IlePro: 3.153 ± 0.047
3.036IleGln: 3.036 ± 0.048
3.419IleArg: 3.419 ± 0.054
4.338IleSer: 4.338 ± 0.06
3.762IleThr: 3.762 ± 0.063
4.517IleVal: 4.517 ± 0.059
0.685IleTrp: 0.685 ± 0.027
2.039IleTyr: 2.039 ± 0.041
0.0IleXaa: 0.0 ± 0.0
Lys
4.271LysAla: 4.271 ± 0.064
0.241LysCys: 0.241 ± 0.014
2.875LysAsp: 2.875 ± 0.058
4.332LysGlu: 4.332 ± 0.051
1.333LysPhe: 1.333 ± 0.033
3.505LysGly: 3.505 ± 0.054
1.154LysHis: 1.154 ± 0.032
2.621LysIle: 2.621 ± 0.051
2.942LysLys: 2.942 ± 0.057
5.188LysLeu: 5.188 ± 0.063
1.438LysMet: 1.438 ± 0.034
1.849LysAsn: 1.849 ± 0.047
2.336LysPro: 2.336 ± 0.048
2.562LysGln: 2.562 ± 0.046
2.745LysArg: 2.745 ± 0.054
2.952LysSer: 2.952 ± 0.052
2.508LysThr: 2.508 ± 0.053
3.402LysVal: 3.402 ± 0.062
0.64LysTrp: 0.64 ± 0.025
1.661LysTyr: 1.661 ± 0.04
0.0LysXaa: 0.0 ± 0.0
Leu
8.084LeuAla: 8.084 ± 0.098
0.807LeuCys: 0.807 ± 0.028
5.201LeuAsp: 5.201 ± 0.072
6.918LeuGlu: 6.918 ± 0.09
4.076LeuPhe: 4.076 ± 0.075
6.871LeuGly: 6.871 ± 0.084
2.258LeuHis: 2.258 ± 0.047
6.19LeuIle: 6.19 ± 0.082
5.436LeuLys: 5.436 ± 0.072
11.394LeuLeu: 11.394 ± 0.14
2.788LeuMet: 2.788 ± 0.053
3.822LeuAsn: 3.822 ± 0.063
4.689LeuPro: 4.689 ± 0.067
4.811LeuGln: 4.811 ± 0.078
5.045LeuArg: 5.045 ± 0.077
7.671LeuSer: 7.671 ± 0.089
5.366LeuThr: 5.366 ± 0.068
6.123LeuVal: 6.123 ± 0.079
1.103LeuTrp: 1.103 ± 0.033
3.242LeuTyr: 3.242 ± 0.046
0.0LeuXaa: 0.0 ± 0.0
Met
2.256MetAla: 2.256 ± 0.046
0.168MetCys: 0.168 ± 0.013
1.673MetAsp: 1.673 ± 0.035
2.095MetGlu: 2.095 ± 0.039
1.065MetPhe: 1.065 ± 0.026
1.767MetGly: 1.767 ± 0.042
0.501MetHis: 0.501 ± 0.019
1.991MetIle: 1.991 ± 0.045
2.123MetLys: 2.123 ± 0.04
3.127MetLeu: 3.127 ± 0.054
1.015MetMet: 1.015 ± 0.034
1.694MetAsn: 1.694 ± 0.038
1.178MetPro: 1.178 ± 0.033
1.106MetGln: 1.106 ± 0.029
1.311MetArg: 1.311 ± 0.034
2.046MetSer: 2.046 ± 0.04
1.77MetThr: 1.77 ± 0.036
1.876MetVal: 1.876 ± 0.035
0.237MetTrp: 0.237 ± 0.013
0.83MetTyr: 0.83 ± 0.025
0.0MetXaa: 0.0 ± 0.0
Asn
2.688AsnAla: 2.688 ± 0.051
0.217AsnCys: 0.217 ± 0.014
1.721AsnAsp: 1.721 ± 0.041
2.484AsnGlu: 2.484 ± 0.053
1.109AsnPhe: 1.109 ± 0.033
2.92AsnGly: 2.92 ± 0.062
0.82AsnHis: 0.82 ± 0.025
2.147AsnIle: 2.147 ± 0.039
1.771AsnLys: 1.771 ± 0.045
3.019AsnLeu: 3.019 ± 0.056
1.045AsnMet: 1.045 ± 0.031
1.43AsnAsn: 1.43 ± 0.046
2.076AsnPro: 2.076 ± 0.041
1.528AsnGln: 1.528 ± 0.035
1.995AsnArg: 1.995 ± 0.044
2.035AsnSer: 2.035 ± 0.051
1.872AsnThr: 1.872 ± 0.044
2.409AsnVal: 2.409 ± 0.047
0.45AsnTrp: 0.45 ± 0.02
1.178AsnTyr: 1.178 ± 0.034
0.0AsnXaa: 0.0 ± 0.0
Pro
3.839ProAla: 3.839 ± 0.063
0.283ProCys: 0.283 ± 0.015
2.654ProAsp: 2.654 ± 0.048
3.855ProGlu: 3.855 ± 0.061
1.805ProPhe: 1.805 ± 0.037
3.368ProGly: 3.368 ± 0.058
0.938ProHis: 0.938 ± 0.026
2.137ProIle: 2.137 ± 0.04
1.802ProLys: 1.802 ± 0.038
4.099ProLeu: 4.099 ± 0.067
1.025ProMet: 1.025 ± 0.03
1.446ProAsn: 1.446 ± 0.045
1.473ProPro: 1.473 ± 0.043
1.72ProGln: 1.72 ± 0.045
1.637ProArg: 1.637 ± 0.037
2.787ProSer: 2.787 ± 0.057
1.68ProThr: 1.68 ± 0.039
3.541ProVal: 3.541 ± 0.056
0.569ProTrp: 0.569 ± 0.022
1.547ProTyr: 1.547 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
3.847GlnAla: 3.847 ± 0.063
0.267GlnCys: 0.267 ± 0.014
2.188GlnAsp: 2.188 ± 0.043
3.613GlnGlu: 3.613 ± 0.067
1.496GlnPhe: 1.496 ± 0.035
3.287GlnGly: 3.287 ± 0.054
1.118GlnHis: 1.118 ± 0.03
2.436GlnIle: 2.436 ± 0.044
2.029GlnLys: 2.029 ± 0.043
4.676GlnLeu: 4.676 ± 0.069
1.264GlnMet: 1.264 ± 0.035
1.359GlnAsn: 1.359 ± 0.033
1.814GlnPro: 1.814 ± 0.038
2.446GlnGln: 2.446 ± 0.059
2.335GlnArg: 2.335 ± 0.045
2.661GlnSer: 2.661 ± 0.052
1.892GlnThr: 1.892 ± 0.044
2.855GlnVal: 2.855 ± 0.047
0.566GlnTrp: 0.566 ± 0.021
1.516GlnTyr: 1.516 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
3.459ArgAla: 3.459 ± 0.055
0.407ArgCys: 0.407 ± 0.02
2.549ArgAsp: 2.549 ± 0.049
4.06ArgGlu: 4.06 ± 0.072
2.166ArgPhe: 2.166 ± 0.04
3.141ArgGly: 3.141 ± 0.053
1.246ArgHis: 1.246 ± 0.031
3.493ArgIle: 3.493 ± 0.059
3.03ArgLys: 3.03 ± 0.054
5.714ArgLeu: 5.714 ± 0.087
1.74ArgMet: 1.74 ± 0.039
1.914ArgAsn: 1.914 ± 0.043
1.914ArgPro: 1.914 ± 0.038
2.283ArgGln: 2.283 ± 0.044
3.135ArgArg: 3.135 ± 0.065
3.616ArgSer: 3.616 ± 0.065
2.705ArgThr: 2.705 ± 0.049
3.2ArgVal: 3.2 ± 0.056
0.7ArgTrp: 0.7 ± 0.027
2.034ArgTyr: 2.034 ± 0.046
0.0ArgXaa: 0.0 ± 0.0
Ser
4.962SerAla: 4.962 ± 0.069
0.508SerCys: 0.508 ± 0.021
3.077SerAsp: 3.077 ± 0.051
3.895SerGlu: 3.895 ± 0.056
3.003SerPhe: 3.003 ± 0.054
5.669SerGly: 5.669 ± 0.071
1.409SerHis: 1.409 ± 0.041
4.285SerIle: 4.285 ± 0.068
3.118SerLys: 3.118 ± 0.046
6.816SerLeu: 6.816 ± 0.089
2.116SerMet: 2.116 ± 0.041
2.165SerAsn: 2.165 ± 0.047
2.69SerPro: 2.69 ± 0.05
2.436SerGln: 2.436 ± 0.043
3.83SerArg: 3.83 ± 0.065
5.119SerSer: 5.119 ± 0.087
3.27SerThr: 3.27 ± 0.054
4.445SerVal: 4.445 ± 0.068
0.855SerTrp: 0.855 ± 0.026
2.287SerTyr: 2.287 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
4.698ThrAla: 4.698 ± 0.069
0.332ThrCys: 0.332 ± 0.015
2.534ThrAsp: 2.534 ± 0.051
3.235ThrGlu: 3.235 ± 0.052
2.134ThrPhe: 2.134 ± 0.038
4.643ThrGly: 4.643 ± 0.058
1.044ThrHis: 1.044 ± 0.031
3.035ThrIle: 3.035 ± 0.054
2.087ThrLys: 2.087 ± 0.046
5.31ThrLeu: 5.31 ± 0.066
1.376ThrMet: 1.376 ± 0.03
1.558ThrAsn: 1.558 ± 0.042
2.563ThrPro: 2.563 ± 0.04
1.635ThrGln: 1.635 ± 0.037
2.444ThrArg: 2.444 ± 0.052
3.267ThrSer: 3.267 ± 0.059
2.568ThrThr: 2.568 ± 0.048
4.234ThrVal: 4.234 ± 0.074
0.595ThrTrp: 0.595 ± 0.023
1.8ThrTyr: 1.8 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
4.484ValAla: 4.484 ± 0.064
0.602ValCys: 0.602 ± 0.021
3.48ValAsp: 3.48 ± 0.055
4.587ValGlu: 4.587 ± 0.072
2.865ValPhe: 2.865 ± 0.058
4.025ValGly: 4.025 ± 0.067
1.686ValHis: 1.686 ± 0.04
4.969ValIle: 4.969 ± 0.061
3.842ValLys: 3.842 ± 0.061
7.484ValLeu: 7.484 ± 0.081
2.153ValMet: 2.153 ± 0.044
2.741ValAsn: 2.741 ± 0.049
3.049ValPro: 3.049 ± 0.055
3.037ValGln: 3.037 ± 0.051
3.436ValArg: 3.436 ± 0.053
4.781ValSer: 4.781 ± 0.063
4.357ValThr: 4.357 ± 0.065
4.737ValVal: 4.737 ± 0.07
0.823ValTrp: 0.823 ± 0.026
2.48ValTyr: 2.48 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
0.823TrpAla: 0.823 ± 0.027
0.118TrpCys: 0.118 ± 0.012
0.662TrpAsp: 0.662 ± 0.022
0.832TrpGlu: 0.832 ± 0.025
0.573TrpPhe: 0.573 ± 0.02
0.816TrpGly: 0.816 ± 0.028
0.266TrpHis: 0.266 ± 0.016
0.877TrpIle: 0.877 ± 0.03
0.744TrpLys: 0.744 ± 0.024
1.395TrpLeu: 1.395 ± 0.039
0.512TrpMet: 0.512 ± 0.024
0.619TrpAsn: 0.619 ± 0.022
0.345TrpPro: 0.345 ± 0.018
0.434TrpGln: 0.434 ± 0.019
0.65TrpArg: 0.65 ± 0.022
0.903TrpSer: 0.903 ± 0.031
0.642TrpThr: 0.642 ± 0.023
0.791TrpVal: 0.791 ± 0.028
0.168TrpTrp: 0.168 ± 0.011
0.395TrpTyr: 0.395 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.559TyrAla: 2.559 ± 0.047
0.309TyrCys: 0.309 ± 0.018
1.86TyrAsp: 1.86 ± 0.047
2.415TyrGlu: 2.415 ± 0.04
1.496TyrPhe: 1.496 ± 0.033
2.646TyrGly: 2.646 ± 0.051
0.805TyrHis: 0.805 ± 0.027
2.133TyrIle: 2.133 ± 0.041
1.525TyrLys: 1.525 ± 0.039
3.138TyrLeu: 3.138 ± 0.054
0.948TyrMet: 0.948 ± 0.027
1.361TyrAsn: 1.361 ± 0.037
1.572TyrPro: 1.572 ± 0.039
1.355TyrGln: 1.355 ± 0.032
2.173TyrArg: 2.173 ± 0.045
2.147TyrSer: 2.147 ± 0.034
1.924TyrThr: 1.924 ± 0.044
2.314TyrVal: 2.314 ± 0.04
0.444TyrTrp: 0.444 ± 0.02
1.334TyrTyr: 1.334 ± 0.037
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4162 proteins (1276428 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski