Streptococcus sp. sy004
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1182 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C5TCM1|A0A5C5TCM1_9STRE GTP-sensing transcriptional pleiotropic repressor CodY OS=Streptococcus sp. sy004 OX=2600149 GN=codY PE=3 SV=1
MM1 pKa = 7.35 EE2 pKa = 5.55 WIEE5 pKa = 4.13 IKK7 pKa = 9.53 TRR9 pKa = 11.84 PITDD13 pKa = 3.82 EE14 pKa = 3.92 EE15 pKa = 4.55 QEE17 pKa = 4.32 LYY19 pKa = 10.17 PYY21 pKa = 9.63 LDD23 pKa = 4.2 CMFDD27 pKa = 3.64 CQVPDD32 pKa = 3.21 VFEE35 pKa = 4.36 NVLVTLSDD43 pKa = 3.32 GRR45 pKa = 11.84 IDD47 pKa = 3.93 VDD49 pKa = 3.43 MFDD52 pKa = 4.07 DD53 pKa = 3.79 YY54 pKa = 11.69 GYY56 pKa = 11.04 GGVGFSEE63 pKa = 4.42 YY64 pKa = 10.77 DD65 pKa = 3.38 DD66 pKa = 6.17 DD67 pKa = 5.27 VIAWMPLPVPFRR79 pKa = 11.84 KK80 pKa = 9.39 EE81 pKa = 3.46
Molecular weight: 9.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.755
IPC2_protein 3.63
IPC_protein 3.592
Toseland 3.376
ProMoST 3.745
Dawson 3.592
Bjellqvist 3.808
Wikipedia 3.554
Rodwell 3.427
Grimsley 3.3
Solomon 3.567
Lehninger 3.528
Nozaki 3.719
DTASelect 3.935
Thurlkill 3.452
EMBOSS 3.554
Sillero 3.719
Patrickios 0.769
IPC_peptide 3.567
IPC2_peptide 3.681
IPC2.peptide.svr19 3.707
Protein with the highest isoelectric point:
>tr|A0A5C5TCX9|A0A5C5TCX9_9STRE ABC transporter permease OS=Streptococcus sp. sy004 OX=2600149 GN=FRX54_00170 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.67 IRR11 pKa = 11.84 RR12 pKa = 11.84 QRR14 pKa = 11.84 KK15 pKa = 7.52 HH16 pKa = 5.2 GFRR19 pKa = 11.84 HH20 pKa = 6.39 RR21 pKa = 11.84 MSTKK25 pKa = 9.02 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.73 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLSAA44 pKa = 4.05
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1182
0
1182
362461
33
1721
306.7
34.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.676 ± 0.078
0.476 ± 0.018
5.697 ± 0.057
6.761 ± 0.08
4.319 ± 0.061
6.387 ± 0.077
1.748 ± 0.03
6.849 ± 0.068
6.557 ± 0.061
10.652 ± 0.123
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.476 ± 0.032
4.331 ± 0.048
3.259 ± 0.042
4.8 ± 0.059
4.08 ± 0.057
6.299 ± 0.068
5.974 ± 0.066
7.168 ± 0.077
0.814 ± 0.024
3.677 ± 0.052
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here