Arthrobacter phage Ingrid

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gordonvirus; unclassified Gordonvirus

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TG02|A0A5J6TG02_9CAUD Uncharacterized protein OS=Arthrobacter phage Ingrid OX=2599825 GN=24 PE=4 SV=1
MM1 pKa = 6.71GTLYY5 pKa = 10.55EE6 pKa = 4.92DD7 pKa = 4.34PNDD10 pKa = 4.03PGTYY14 pKa = 10.03LIQSTPSTPSDD25 pKa = 3.34PGGGYY30 pKa = 10.63DD31 pKa = 3.44PTRR34 pKa = 11.84PTDD37 pKa = 3.71LRR39 pKa = 11.84PTKK42 pKa = 9.65LTWGTLGEE50 pKa = 4.17RR51 pKa = 11.84FYY53 pKa = 11.33EE54 pKa = 4.49AGTDD58 pKa = 3.3RR59 pKa = 11.84GVLYY63 pKa = 10.99VDD65 pKa = 3.72GVGYY69 pKa = 10.6AWNGLVSVNQTPSGGEE85 pKa = 3.78PEE87 pKa = 4.77PYY89 pKa = 10.11YY90 pKa = 10.91LDD92 pKa = 4.59GIPYY96 pKa = 10.03VMAASPEE103 pKa = 4.09EE104 pKa = 3.97FKK106 pKa = 11.37GSIEE110 pKa = 4.24AYY112 pKa = 7.97TYY114 pKa = 10.16PKK116 pKa = 10.65AFEE119 pKa = 4.51ACDD122 pKa = 3.67GSEE125 pKa = 4.47EE126 pKa = 4.2IAQGLYY132 pKa = 9.99ISQQNRR138 pKa = 11.84KK139 pKa = 8.69PFGFSYY145 pKa = 9.32RR146 pKa = 11.84TLVGNDD152 pKa = 2.91IDD154 pKa = 4.71EE155 pKa = 4.98LDD157 pKa = 3.59HH158 pKa = 6.63GYY160 pKa = 10.12KK161 pKa = 9.98VHH163 pKa = 6.84IVYY166 pKa = 10.26EE167 pKa = 4.48AMASPSNRR175 pKa = 11.84NHH177 pKa = 5.93GTLGDD182 pKa = 3.9NTDD185 pKa = 3.36PTTFSWNIITKK196 pKa = 8.86PVKK199 pKa = 10.64FEE201 pKa = 4.63DD202 pKa = 3.55PAFGIKK208 pKa = 10.13YY209 pKa = 9.38GAHH212 pKa = 5.95LTLDD216 pKa = 3.52SRR218 pKa = 11.84EE219 pKa = 4.11VYY221 pKa = 8.57PWAMAAVEE229 pKa = 4.08DD230 pKa = 4.13VLYY233 pKa = 8.91GTEE236 pKa = 4.04DD237 pKa = 3.53TEE239 pKa = 4.49PRR241 pKa = 11.84LPTPQEE247 pKa = 3.65IVALFADD254 pKa = 3.82NALLKK259 pKa = 9.82ITDD262 pKa = 4.11NGDD265 pKa = 3.66GTWTAEE271 pKa = 4.3GPDD274 pKa = 4.55SIISMIDD281 pKa = 2.94ANTFQIDD288 pKa = 3.67WPSAVYY294 pKa = 10.57LDD296 pKa = 3.9EE297 pKa = 3.96NTYY300 pKa = 10.9NVSSLL305 pKa = 3.48

Molecular weight: 33.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TEI5|A0A5J6TEI5_9CAUD Uncharacterized protein OS=Arthrobacter phage Ingrid OX=2599825 GN=72 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 10.2NLKK5 pKa = 9.96RR6 pKa = 11.84FAWLLRR12 pKa = 11.84MTPWVFKK19 pKa = 10.37HH20 pKa = 6.46AYY22 pKa = 8.13HH23 pKa = 7.31APNHH27 pKa = 5.18QVNGSSIEE35 pKa = 4.0KK36 pKa = 10.38NFVLGYY42 pKa = 8.13FRR44 pKa = 11.84CRR46 pKa = 11.84RR47 pKa = 11.84CQEE50 pKa = 4.08TLTVPSYY57 pKa = 11.03ISNN60 pKa = 3.7

Molecular weight: 7.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

18135

38

1528

190.9

21.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.839 ± 0.471

0.612 ± 0.102

5.729 ± 0.316

6.479 ± 0.372

3.794 ± 0.177

7.588 ± 0.527

1.908 ± 0.196

5.961 ± 0.221

6.573 ± 0.411

7.825 ± 0.372

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.093 ± 0.214

4.808 ± 0.151

4.119 ± 0.247

3.309 ± 0.112

4.968 ± 0.281

6.369 ± 0.237

6.248 ± 0.255

6.964 ± 0.221

1.467 ± 0.131

3.347 ± 0.266
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski