Amino acid dipepetide frequency for Arthrobacter phage Ingrid

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.547AlaAla: 8.547 ± 1.284
0.386AlaCys: 0.386 ± 0.141
4.963AlaAsp: 4.963 ± 0.492
5.57AlaGlu: 5.57 ± 0.537
3.143AlaPhe: 3.143 ± 0.366
6.011AlaGly: 6.011 ± 1.051
1.93AlaHis: 1.93 ± 0.38
5.57AlaIle: 5.57 ± 0.634
5.735AlaLys: 5.735 ± 0.902
6.948AlaLeu: 6.948 ± 0.768
3.143AlaMet: 3.143 ± 0.656
4.412AlaAsn: 4.412 ± 0.495
2.868AlaPro: 2.868 ± 0.378
2.923AlaGln: 2.923 ± 0.397
4.467AlaArg: 4.467 ± 0.511
5.184AlaSer: 5.184 ± 0.637
6.011AlaThr: 6.011 ± 0.567
6.287AlaVal: 6.287 ± 0.743
1.323AlaTrp: 1.323 ± 0.264
2.923AlaTyr: 2.923 ± 0.344
0.0AlaXaa: 0.0 ± 0.0
Cys
0.441CysAla: 0.441 ± 0.163
0.165CysCys: 0.165 ± 0.111
0.496CysAsp: 0.496 ± 0.153
0.551CysGlu: 0.551 ± 0.196
0.276CysPhe: 0.276 ± 0.138
0.551CysGly: 0.551 ± 0.212
0.221CysHis: 0.221 ± 0.119
0.496CysIle: 0.496 ± 0.208
0.165CysLys: 0.165 ± 0.097
0.607CysLeu: 0.607 ± 0.223
0.11CysMet: 0.11 ± 0.067
0.165CysAsn: 0.165 ± 0.103
0.331CysPro: 0.331 ± 0.143
0.11CysGln: 0.11 ± 0.074
0.386CysArg: 0.386 ± 0.164
0.386CysSer: 0.386 ± 0.159
0.276CysThr: 0.276 ± 0.138
0.221CysVal: 0.221 ± 0.109
0.11CysTrp: 0.11 ± 0.078
0.055CysTyr: 0.055 ± 0.058
0.0CysXaa: 0.0 ± 0.0
Asp
5.956AspAla: 5.956 ± 0.598
0.221AspCys: 0.221 ± 0.106
3.088AspAsp: 3.088 ± 0.418
4.246AspGlu: 4.246 ± 0.45
2.04AspPhe: 2.04 ± 0.496
4.963AspGly: 4.963 ± 0.498
0.717AspHis: 0.717 ± 0.212
3.75AspIle: 3.75 ± 0.557
2.978AspLys: 2.978 ± 0.388
4.246AspLeu: 4.246 ± 0.498
1.82AspMet: 1.82 ± 0.31
3.088AspAsn: 3.088 ± 0.524
2.868AspPro: 2.868 ± 0.464
1.93AspGln: 1.93 ± 0.487
2.868AspArg: 2.868 ± 0.497
3.254AspSer: 3.254 ± 0.347
2.757AspThr: 2.757 ± 0.365
4.191AspVal: 4.191 ± 0.661
0.772AspTrp: 0.772 ± 0.206
1.544AspTyr: 1.544 ± 0.302
0.0AspXaa: 0.0 ± 0.0
Glu
4.632GluAla: 4.632 ± 0.581
0.441GluCys: 0.441 ± 0.174
4.136GluAsp: 4.136 ± 0.585
5.349GluGlu: 5.349 ± 0.765
2.923GluPhe: 2.923 ± 0.414
4.246GluGly: 4.246 ± 0.481
1.544GluHis: 1.544 ± 0.329
4.301GluIle: 4.301 ± 0.431
3.97GluLys: 3.97 ± 0.515
4.853GluLeu: 4.853 ± 0.63
2.702GluMet: 2.702 ± 0.38
2.812GluAsn: 2.812 ± 0.418
2.702GluPro: 2.702 ± 0.382
2.151GluGln: 2.151 ± 0.348
3.419GluArg: 3.419 ± 0.454
3.088GluSer: 3.088 ± 0.424
3.915GluThr: 3.915 ± 0.575
4.963GluVal: 4.963 ± 0.679
1.158GluTrp: 1.158 ± 0.24
1.489GluTyr: 1.489 ± 0.301
0.0GluXaa: 0.0 ± 0.0
Phe
2.702PheAla: 2.702 ± 0.379
0.165PheCys: 0.165 ± 0.09
2.537PheAsp: 2.537 ± 0.395
2.371PheGlu: 2.371 ± 0.412
1.268PhePhe: 1.268 ± 0.319
2.978PheGly: 2.978 ± 0.34
0.607PheHis: 0.607 ± 0.191
1.765PheIle: 1.765 ± 0.335
2.537PheLys: 2.537 ± 0.31
3.254PheLeu: 3.254 ± 0.379
1.048PheMet: 1.048 ± 0.261
2.151PheAsn: 2.151 ± 0.331
1.489PhePro: 1.489 ± 0.354
1.158PheGln: 1.158 ± 0.235
2.316PheArg: 2.316 ± 0.391
2.702PheSer: 2.702 ± 0.363
2.261PheThr: 2.261 ± 0.387
2.812PheVal: 2.812 ± 0.395
0.441PheTrp: 0.441 ± 0.165
1.379PheTyr: 1.379 ± 0.324
0.0PheXaa: 0.0 ± 0.0
Gly
6.397GlyAla: 6.397 ± 0.674
0.221GlyCys: 0.221 ± 0.135
4.687GlyAsp: 4.687 ± 0.622
4.853GlyGlu: 4.853 ± 0.468
3.75GlyPhe: 3.75 ± 0.524
6.617GlyGly: 6.617 ± 1.598
1.158GlyHis: 1.158 ± 0.25
4.412GlyIle: 4.412 ± 0.601
4.963GlyLys: 4.963 ± 0.461
6.287GlyLeu: 6.287 ± 1.119
2.537GlyMet: 2.537 ± 0.577
2.978GlyAsn: 2.978 ± 0.373
1.82GlyPro: 1.82 ± 0.307
1.709GlyGln: 1.709 ± 0.33
2.482GlyArg: 2.482 ± 0.309
4.908GlySer: 4.908 ± 0.66
6.066GlyThr: 6.066 ± 0.778
5.735GlyVal: 5.735 ± 0.673
1.599GlyTrp: 1.599 ± 0.316
2.702GlyTyr: 2.702 ± 0.487
0.0GlyXaa: 0.0 ± 0.0
His
1.93HisAla: 1.93 ± 0.467
0.276HisCys: 0.276 ± 0.146
0.882HisAsp: 0.882 ± 0.236
1.379HisGlu: 1.379 ± 0.326
0.607HisPhe: 0.607 ± 0.195
1.379HisGly: 1.379 ± 0.34
0.496HisHis: 0.496 ± 0.14
1.434HisIle: 1.434 ± 0.302
1.103HisLys: 1.103 ± 0.233
1.048HisLeu: 1.048 ± 0.186
0.496HisMet: 0.496 ± 0.16
0.717HisAsn: 0.717 ± 0.264
1.048HisPro: 1.048 ± 0.211
0.772HisGln: 0.772 ± 0.199
0.772HisArg: 0.772 ± 0.238
1.158HisSer: 1.158 ± 0.3
0.772HisThr: 0.772 ± 0.272
1.213HisVal: 1.213 ± 0.276
0.165HisTrp: 0.165 ± 0.092
1.434HisTyr: 1.434 ± 0.285
0.0HisXaa: 0.0 ± 0.0
Ile
5.459IleAla: 5.459 ± 1.026
0.331IleCys: 0.331 ± 0.166
4.026IleAsp: 4.026 ± 0.399
3.86IleGlu: 3.86 ± 0.483
1.544IlePhe: 1.544 ± 0.318
3.915IleGly: 3.915 ± 0.529
1.158IleHis: 1.158 ± 0.228
2.702IleIle: 2.702 ± 0.386
4.246IleLys: 4.246 ± 0.579
5.128IleLeu: 5.128 ± 0.585
1.765IleMet: 1.765 ± 0.267
3.143IleAsn: 3.143 ± 0.376
2.923IlePro: 2.923 ± 0.42
1.709IleGln: 1.709 ± 0.286
2.757IleArg: 2.757 ± 0.391
3.97IleSer: 3.97 ± 0.452
4.026IleThr: 4.026 ± 0.463
4.742IleVal: 4.742 ± 0.451
0.662IleTrp: 0.662 ± 0.193
1.544IleTyr: 1.544 ± 0.228
0.0IleXaa: 0.0 ± 0.0
Lys
5.901LysAla: 5.901 ± 0.659
0.331LysCys: 0.331 ± 0.12
2.923LysAsp: 2.923 ± 0.623
4.963LysGlu: 4.963 ± 0.595
2.647LysPhe: 2.647 ± 0.373
4.632LysGly: 4.632 ± 0.57
1.379LysHis: 1.379 ± 0.309
3.309LysIle: 3.309 ± 0.477
5.735LysLys: 5.735 ± 0.834
5.901LysLeu: 5.901 ± 0.582
2.592LysMet: 2.592 ± 0.371
2.04LysAsn: 2.04 ± 0.38
2.978LysPro: 2.978 ± 0.396
2.261LysGln: 2.261 ± 0.376
4.026LysArg: 4.026 ± 0.546
4.026LysSer: 4.026 ± 0.516
3.64LysThr: 3.64 ± 0.369
3.64LysVal: 3.64 ± 0.454
1.048LysTrp: 1.048 ± 0.259
1.765LysTyr: 1.765 ± 0.291
0.0LysXaa: 0.0 ± 0.0
Leu
7.059LeuAla: 7.059 ± 0.922
0.386LeuCys: 0.386 ± 0.159
5.073LeuAsp: 5.073 ± 0.573
4.853LeuGlu: 4.853 ± 0.558
3.033LeuPhe: 3.033 ± 0.401
6.011LeuGly: 6.011 ± 1.07
1.489LeuHis: 1.489 ± 0.298
6.121LeuIle: 6.121 ± 0.612
5.459LeuLys: 5.459 ± 0.692
5.294LeuLeu: 5.294 ± 0.822
1.93LeuMet: 1.93 ± 0.345
4.026LeuAsn: 4.026 ± 0.431
3.254LeuPro: 3.254 ± 0.384
2.261LeuGln: 2.261 ± 0.347
3.695LeuArg: 3.695 ± 0.468
5.239LeuSer: 5.239 ± 0.456
4.742LeuThr: 4.742 ± 0.522
5.184LeuVal: 5.184 ± 0.531
0.772LeuTrp: 0.772 ± 0.219
2.371LeuTyr: 2.371 ± 0.429
0.0LeuXaa: 0.0 ± 0.0
Met
3.474MetAla: 3.474 ± 0.438
0.221MetCys: 0.221 ± 0.156
1.213MetAsp: 1.213 ± 0.22
1.268MetGlu: 1.268 ± 0.227
1.048MetPhe: 1.048 ± 0.285
2.592MetGly: 2.592 ± 0.565
0.441MetHis: 0.441 ± 0.144
2.04MetIle: 2.04 ± 0.438
1.93MetLys: 1.93 ± 0.347
2.537MetLeu: 2.537 ± 0.38
1.103MetMet: 1.103 ± 0.403
1.158MetAsn: 1.158 ± 0.242
1.379MetPro: 1.379 ± 0.232
0.827MetGln: 0.827 ± 0.331
1.158MetArg: 1.158 ± 0.306
3.364MetSer: 3.364 ± 0.418
2.206MetThr: 2.206 ± 0.382
1.709MetVal: 1.709 ± 0.312
0.386MetTrp: 0.386 ± 0.155
0.882MetTyr: 0.882 ± 0.205
0.0MetXaa: 0.0 ± 0.0
Asn
3.529AsnAla: 3.529 ± 0.526
0.386AsnCys: 0.386 ± 0.193
2.923AsnAsp: 2.923 ± 0.393
3.254AsnGlu: 3.254 ± 0.475
1.599AsnPhe: 1.599 ± 0.276
4.687AsnGly: 4.687 ± 0.638
0.937AsnHis: 0.937 ± 0.247
2.316AsnIle: 2.316 ± 0.492
2.757AsnLys: 2.757 ± 0.347
3.419AsnLeu: 3.419 ± 0.44
1.379AsnMet: 1.379 ± 0.326
2.096AsnAsn: 2.096 ± 0.413
2.978AsnPro: 2.978 ± 0.399
1.93AsnGln: 1.93 ± 0.346
2.371AsnArg: 2.371 ± 0.391
3.364AsnSer: 3.364 ± 0.381
2.923AsnThr: 2.923 ± 0.368
2.757AsnVal: 2.757 ± 0.383
0.717AsnTrp: 0.717 ± 0.261
1.765AsnTyr: 1.765 ± 0.332
0.0AsnXaa: 0.0 ± 0.0
Pro
3.143ProAla: 3.143 ± 0.448
0.496ProCys: 0.496 ± 0.18
2.04ProAsp: 2.04 ± 0.343
3.86ProGlu: 3.86 ± 0.622
1.048ProPhe: 1.048 ± 0.231
3.474ProGly: 3.474 ± 0.502
0.717ProHis: 0.717 ± 0.205
2.151ProIle: 2.151 ± 0.268
2.757ProLys: 2.757 ± 0.429
2.151ProLeu: 2.151 ± 0.307
1.048ProMet: 1.048 ± 0.241
2.096ProAsn: 2.096 ± 0.524
1.489ProPro: 1.489 ± 0.351
0.882ProGln: 0.882 ± 0.221
1.985ProArg: 1.985 ± 0.296
3.198ProSer: 3.198 ± 0.374
2.923ProThr: 2.923 ± 0.375
4.081ProVal: 4.081 ± 0.569
0.386ProTrp: 0.386 ± 0.106
1.268ProTyr: 1.268 ± 0.295
0.0ProXaa: 0.0 ± 0.0
Gln
2.482GlnAla: 2.482 ± 0.421
0.221GlnCys: 0.221 ± 0.116
1.599GlnAsp: 1.599 ± 0.379
2.151GlnGlu: 2.151 ± 0.432
1.379GlnPhe: 1.379 ± 0.242
2.371GlnGly: 2.371 ± 0.415
0.772GlnHis: 0.772 ± 0.23
1.599GlnIle: 1.599 ± 0.283
2.206GlnLys: 2.206 ± 0.309
3.364GlnLeu: 3.364 ± 0.51
0.882GlnMet: 0.882 ± 0.228
1.434GlnAsn: 1.434 ± 0.27
1.103GlnPro: 1.103 ± 0.244
1.765GlnGln: 1.765 ± 0.281
2.206GlnArg: 2.206 ± 0.395
1.103GlnSer: 1.103 ± 0.208
1.875GlnThr: 1.875 ± 0.354
2.482GlnVal: 2.482 ± 0.353
0.607GlnTrp: 0.607 ± 0.172
1.489GlnTyr: 1.489 ± 0.266
0.0GlnXaa: 0.0 ± 0.0
Arg
3.75ArgAla: 3.75 ± 0.443
0.441ArgCys: 0.441 ± 0.168
2.371ArgAsp: 2.371 ± 0.381
3.033ArgGlu: 3.033 ± 0.465
1.434ArgPhe: 1.434 ± 0.271
2.647ArgGly: 2.647 ± 0.304
1.434ArgHis: 1.434 ± 0.309
3.033ArgIle: 3.033 ± 0.348
3.584ArgLys: 3.584 ± 0.513
4.301ArgLeu: 4.301 ± 0.542
1.379ArgMet: 1.379 ± 0.267
2.482ArgAsn: 2.482 ± 0.339
1.434ArgPro: 1.434 ± 0.28
2.482ArgGln: 2.482 ± 0.464
3.529ArgArg: 3.529 ± 0.517
3.254ArgSer: 3.254 ± 0.46
2.812ArgThr: 2.812 ± 0.353
3.805ArgVal: 3.805 ± 0.47
0.551ArgTrp: 0.551 ± 0.177
1.93ArgTyr: 1.93 ± 0.353
0.0ArgXaa: 0.0 ± 0.0
Ser
5.239SerAla: 5.239 ± 0.684
0.165SerCys: 0.165 ± 0.091
2.978SerAsp: 2.978 ± 0.427
3.364SerGlu: 3.364 ± 0.479
2.702SerPhe: 2.702 ± 0.387
5.515SerGly: 5.515 ± 0.479
1.048SerHis: 1.048 ± 0.274
4.026SerIle: 4.026 ± 0.479
4.301SerLys: 4.301 ± 0.557
4.853SerLeu: 4.853 ± 0.524
1.875SerMet: 1.875 ± 0.318
3.529SerAsn: 3.529 ± 0.371
2.868SerPro: 2.868 ± 0.43
2.096SerGln: 2.096 ± 0.3
3.198SerArg: 3.198 ± 0.433
4.798SerSer: 4.798 ± 0.653
4.412SerThr: 4.412 ± 0.556
2.923SerVal: 2.923 ± 0.274
1.434SerTrp: 1.434 ± 0.265
2.371SerTyr: 2.371 ± 0.381
0.0SerXaa: 0.0 ± 0.0
Thr
5.459ThrAla: 5.459 ± 0.726
0.717ThrCys: 0.717 ± 0.246
3.695ThrAsp: 3.695 ± 0.457
3.419ThrGlu: 3.419 ± 0.564
2.702ThrPhe: 2.702 ± 0.369
5.184ThrGly: 5.184 ± 0.516
0.993ThrHis: 0.993 ± 0.264
3.915ThrIle: 3.915 ± 0.527
3.695ThrLys: 3.695 ± 0.441
5.459ThrLeu: 5.459 ± 0.522
1.709ThrMet: 1.709 ± 0.342
2.647ThrAsn: 2.647 ± 0.322
3.75ThrPro: 3.75 ± 0.493
1.985ThrGln: 1.985 ± 0.328
2.04ThrArg: 2.04 ± 0.289
3.088ThrSer: 3.088 ± 0.449
4.356ThrThr: 4.356 ± 0.523
4.467ThrVal: 4.467 ± 0.528
1.048ThrTrp: 1.048 ± 0.288
2.151ThrTyr: 2.151 ± 0.336
0.0ThrXaa: 0.0 ± 0.0
Val
7.224ValAla: 7.224 ± 0.657
0.11ValCys: 0.11 ± 0.084
4.136ValAsp: 4.136 ± 0.381
4.026ValGlu: 4.026 ± 0.547
2.647ValPhe: 2.647 ± 0.397
4.467ValGly: 4.467 ± 0.494
0.993ValHis: 0.993 ± 0.238
4.081ValIle: 4.081 ± 0.405
4.908ValLys: 4.908 ± 0.605
5.68ValLeu: 5.68 ± 0.689
1.985ValMet: 1.985 ± 0.316
4.081ValAsn: 4.081 ± 0.454
2.702ValPro: 2.702 ± 0.393
2.206ValGln: 2.206 ± 0.389
3.695ValArg: 3.695 ± 0.491
4.798ValSer: 4.798 ± 0.497
3.198ValThr: 3.198 ± 0.421
5.073ValVal: 5.073 ± 0.54
0.827ValTrp: 0.827 ± 0.183
2.812ValTyr: 2.812 ± 0.459
0.0ValXaa: 0.0 ± 0.0
Trp
1.434TrpAla: 1.434 ± 0.32
0.11TrpCys: 0.11 ± 0.086
1.213TrpAsp: 1.213 ± 0.286
0.662TrpGlu: 0.662 ± 0.183
0.496TrpPhe: 0.496 ± 0.171
1.489TrpGly: 1.489 ± 0.284
0.331TrpHis: 0.331 ± 0.149
0.607TrpIle: 0.607 ± 0.186
0.662TrpLys: 0.662 ± 0.197
1.048TrpLeu: 1.048 ± 0.2
0.496TrpMet: 0.496 ± 0.147
0.882TrpAsn: 0.882 ± 0.246
0.221TrpPro: 0.221 ± 0.108
0.882TrpGln: 0.882 ± 0.219
0.607TrpArg: 0.607 ± 0.212
0.662TrpSer: 0.662 ± 0.204
1.103TrpThr: 1.103 ± 0.254
1.048TrpVal: 1.048 ± 0.269
0.276TrpTrp: 0.276 ± 0.125
0.441TrpTyr: 0.441 ± 0.149
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.64TyrAla: 3.64 ± 0.45
0.331TyrCys: 0.331 ± 0.145
2.316TyrAsp: 2.316 ± 0.59
1.765TyrGlu: 1.765 ± 0.365
1.654TyrPhe: 1.654 ± 0.287
2.151TyrGly: 2.151 ± 0.278
0.717TyrHis: 0.717 ± 0.211
1.985TyrIle: 1.985 ± 0.333
2.04TyrLys: 2.04 ± 0.325
1.875TyrLeu: 1.875 ± 0.348
0.882TyrMet: 0.882 ± 0.242
2.151TyrAsn: 2.151 ± 0.31
0.993TyrPro: 0.993 ± 0.226
1.048TyrGln: 1.048 ± 0.253
1.709TyrArg: 1.709 ± 0.296
2.151TyrSer: 2.151 ± 0.379
2.206TyrThr: 2.206 ± 0.377
2.316TyrVal: 2.316 ± 0.391
0.386TyrTrp: 0.386 ± 0.2
1.158TyrTyr: 1.158 ± 0.314
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (18135 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski