Haliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Myxococcales; Nannocystineae; Kofleriaceae; Haliangium; Haliangium ochraceum

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6684 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D0LXL5|D0LXL5_HALO1 Small ribosomal subunit biogenesis GTPase RsgA OS=Haliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2) OX=502025 GN=rsgA PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 10.31RR3 pKa = 11.84IFSTTLTLCSLSLLLGACGGDD24 pKa = 3.54DD25 pKa = 3.46EE26 pKa = 6.06LSNEE30 pKa = 4.66DD31 pKa = 3.67YY32 pKa = 11.49DD33 pKa = 5.56DD34 pKa = 3.69IAVGVGSLVAVPGGEE49 pKa = 4.07GGEE52 pKa = 4.16TGSLNDD58 pKa = 3.51VLEE61 pKa = 4.44ASISAGTSAAEE72 pKa = 4.2GASSVEE78 pKa = 4.25IVVHH82 pKa = 6.41AGVSYY87 pKa = 9.83EE88 pKa = 3.88YY89 pKa = 11.06RR90 pKa = 11.84LDD92 pKa = 4.08CYY94 pKa = 10.84DD95 pKa = 4.16AAGALQDD102 pKa = 3.84ACGADD107 pKa = 3.54TDD109 pKa = 4.22SAEE112 pKa = 5.17ASVDD116 pKa = 2.95WSGEE120 pKa = 3.87LDD122 pKa = 3.73TPSYY126 pKa = 10.17DD127 pKa = 3.32ASIMRR132 pKa = 11.84VGDD135 pKa = 3.35WSLSGLQSDD144 pKa = 3.92TAVFSGTGSFDD155 pKa = 3.25IEE157 pKa = 4.51TSFSAMYY164 pKa = 10.27RR165 pKa = 11.84DD166 pKa = 3.86VEE168 pKa = 4.42RR169 pKa = 11.84TLSLSYY175 pKa = 10.47DD176 pKa = 3.24ASYY179 pKa = 11.83DD180 pKa = 4.48DD181 pKa = 3.54ITIDD185 pKa = 2.97IASRR189 pKa = 11.84RR190 pKa = 11.84VTGGAIHH197 pKa = 6.17YY198 pKa = 9.2NIDD201 pKa = 3.05GARR204 pKa = 11.84YY205 pKa = 9.02DD206 pKa = 3.57EE207 pKa = 4.55RR208 pKa = 11.84GARR211 pKa = 11.84TKK213 pKa = 10.36EE214 pKa = 3.41IEE216 pKa = 4.21FAVTADD222 pKa = 4.26LSFTGDD228 pKa = 3.37GEE230 pKa = 4.38ALLVLDD236 pKa = 4.65GSRR239 pKa = 11.84SYY241 pKa = 11.47VVDD244 pKa = 4.51LDD246 pKa = 3.92TGAVAAEE253 pKa = 4.06

Molecular weight: 26.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D0LUI2|D0LUI2_HALO1 SsrA-binding protein OS=Haliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2) OX=502025 GN=smpB PE=3 SV=1
MM1 pKa = 7.01ATGRR5 pKa = 11.84RR6 pKa = 11.84TKK8 pKa = 10.47RR9 pKa = 11.84SLKK12 pKa = 10.16DD13 pKa = 3.49RR14 pKa = 11.84PKK16 pKa = 10.44HH17 pKa = 4.72RR18 pKa = 11.84QARR21 pKa = 11.84ALKK24 pKa = 8.4TRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84KK29 pKa = 9.77AKK31 pKa = 9.36QAASRR36 pKa = 11.84KK37 pKa = 9.2RR38 pKa = 11.84GLNKK42 pKa = 9.73SRR44 pKa = 11.84ARR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84TRR50 pKa = 11.84QGRR53 pKa = 11.84SGKK56 pKa = 10.07RR57 pKa = 11.84VIRR60 pKa = 4.17

Molecular weight: 7.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6684

0

6684

2756058

32

7712

412.3

44.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.572 ± 0.056

1.148 ± 0.02

6.324 ± 0.03

6.704 ± 0.023

3.115 ± 0.017

8.563 ± 0.032

2.234 ± 0.014

3.714 ± 0.022

2.101 ± 0.026

10.364 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.87 ± 0.012

2.002 ± 0.019

5.547 ± 0.022

3.227 ± 0.018

8.336 ± 0.035

5.843 ± 0.021

4.613 ± 0.019

7.244 ± 0.026

1.277 ± 0.012

2.203 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski