Caldilinea aerophila (strain DSM 14535 / JCM 11387 / NBRC 104270 / STL-6-O1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Caldilineae; Caldilineales; Caldilineaceae; Caldilinea; Caldilinea aerophila

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4097 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I0I1K4|I0I1K4_CALAS Putative oligopeptide ABC transporter permease protein OS=Caldilinea aerophila (strain DSM 14535 / JCM 11387 / NBRC 104270 / STL-6-O1) OX=926550 GN=CLDAP_11020 PE=3 SV=1
MM1 pKa = 7.49LALALSALPTPLLAAPFQQEE21 pKa = 4.4VPVSDD26 pKa = 3.86GLTLEE31 pKa = 4.69ALANLTYY38 pKa = 10.37RR39 pKa = 11.84SEE41 pKa = 4.14LTPSGEE47 pKa = 3.74ITLVDD52 pKa = 3.94GRR54 pKa = 11.84YY55 pKa = 8.91EE56 pKa = 4.15DD57 pKa = 3.47PANRR61 pKa = 11.84VFAVLAAEE69 pKa = 4.25PMAFGTINGQDD80 pKa = 3.18AAAVLLAEE88 pKa = 4.33SSGAGSVFITLAIVVDD104 pKa = 3.99QDD106 pKa = 3.86GEE108 pKa = 4.42PVNVASTLLGDD119 pKa = 3.89RR120 pKa = 11.84VDD122 pKa = 4.17VYY124 pKa = 11.56ALEE127 pKa = 4.85IDD129 pKa = 4.19GSRR132 pKa = 11.84ILVDD136 pKa = 3.51MVRR139 pKa = 11.84QGPNDD144 pKa = 3.66PMCCPTEE151 pKa = 4.17VVRR154 pKa = 11.84QIYY157 pKa = 10.6ALGEE161 pKa = 4.03EE162 pKa = 4.55GLILAGVNLIGSGVQAALNVSPEE185 pKa = 3.72GGYY188 pKa = 7.15QTKK191 pKa = 10.2VMPATPYY198 pKa = 10.46AISALIPTGAPIHH211 pKa = 5.87TLLTFGDD218 pKa = 4.67DD219 pKa = 3.69DD220 pKa = 4.82PEE222 pKa = 4.56TVFLNGGPYY231 pKa = 8.91IAIYY235 pKa = 9.26PVQEE239 pKa = 4.46FVRR242 pKa = 11.84LWEE245 pKa = 4.01EE246 pKa = 4.25AGDD249 pKa = 3.72LTIANLVEE257 pKa = 3.93EE258 pKa = 5.15LKK260 pKa = 10.8RR261 pKa = 11.84ILTEE265 pKa = 4.09QPASLAAPLPIVPPADD281 pKa = 3.25VDD283 pKa = 4.17DD284 pKa = 5.35LVAQVVYY291 pKa = 11.05LEE293 pKa = 5.18GEE295 pKa = 4.24DD296 pKa = 3.63FTGVRR301 pKa = 11.84FIARR305 pKa = 11.84SVRR308 pKa = 11.84NGIVSADD315 pKa = 3.41GQLAYY320 pKa = 10.49HH321 pKa = 5.78FTGLTDD327 pKa = 3.72DD328 pKa = 3.5SRR330 pKa = 11.84YY331 pKa = 10.44LIAAQWPVEE340 pKa = 4.19TTAALTALDD349 pKa = 4.39SAPDD353 pKa = 4.02SAWTPALSSLDD364 pKa = 3.61VLIEE368 pKa = 3.97SLTIQGPEE376 pKa = 3.55WVTVEE381 pKa = 3.92EE382 pKa = 4.55LANLTYY388 pKa = 10.82DD389 pKa = 3.27SVMLEE394 pKa = 4.12KK395 pKa = 10.33PVTLSSGVYY404 pKa = 8.63TEE406 pKa = 4.29TVIPDD411 pKa = 3.53VPAGVATVQLLGEE424 pKa = 4.75PIAYY428 pKa = 9.85GKK430 pKa = 11.02VNGLDD435 pKa = 3.34SAAVLLVEE443 pKa = 4.35NTGGTGQFYY452 pKa = 10.31SLKK455 pKa = 9.95LVQRR459 pKa = 11.84IDD461 pKa = 3.54GEE463 pKa = 4.58AVNTASAFLGDD474 pKa = 3.91RR475 pKa = 11.84PRR477 pKa = 11.84IQNIVINEE485 pKa = 4.31DD486 pKa = 2.99GSITVDD492 pKa = 4.74LIQVGPNDD500 pKa = 4.33AFCCANTPMTVDD512 pKa = 4.15FVKK515 pKa = 10.41MGDD518 pKa = 3.28RR519 pKa = 11.84LIVQEE524 pKa = 4.95EE525 pKa = 3.96ISATIDD531 pKa = 3.42LAGLEE536 pKa = 4.67LPLIAAVKK544 pKa = 9.47PATAYY549 pKa = 9.47DD550 pKa = 3.52ASVPPGEE557 pKa = 4.31QGQPKK562 pKa = 9.12HH563 pKa = 6.38FSWVWTDD570 pKa = 4.65DD571 pKa = 3.38EE572 pKa = 4.59TAQGYY577 pKa = 9.74ISVYY581 pKa = 10.03PVEE584 pKa = 5.0AYY586 pKa = 9.62QAIWEE591 pKa = 4.25EE592 pKa = 3.87ADD594 pKa = 3.74DD595 pKa = 4.42PFVTEE600 pKa = 4.11TLAQLRR606 pKa = 11.84ALLEE610 pKa = 4.14EE611 pKa = 4.64QPANPPPPLPFLPPLPASNDD631 pKa = 2.8FAAQVRR637 pKa = 11.84YY638 pKa = 10.39LEE640 pKa = 4.49LADD643 pKa = 4.03GGVGVRR649 pKa = 11.84FIGRR653 pKa = 11.84FVQDD657 pKa = 2.81VAPIEE662 pKa = 4.32NYY664 pKa = 7.18QLRR667 pKa = 11.84YY668 pKa = 9.94LFQGLSADD676 pKa = 3.52GAYY679 pKa = 10.62LIVANLPVSTSALPAEE695 pKa = 4.38PQPLSGEE702 pKa = 4.3EE703 pKa = 3.78YY704 pKa = 9.29DD705 pKa = 3.98QFVAVYY711 pKa = 6.89TTYY714 pKa = 10.6LAEE717 pKa = 4.07MTTLFNGLTSTDD729 pKa = 3.96FTPDD733 pKa = 3.38LDD735 pKa = 4.25ALDD738 pKa = 5.93AILQSVTPKK747 pKa = 9.78RR748 pKa = 11.84TVNPLAPISLANMTINSEE766 pKa = 4.11LASDD770 pKa = 4.82GVAPLANGIYY780 pKa = 9.49TEE782 pKa = 4.58TVAPGSPGLIEE793 pKa = 4.62VRR795 pKa = 11.84LLPEE799 pKa = 3.91PMAYY803 pKa = 10.12GIINEE808 pKa = 4.24QEE810 pKa = 3.8VVAAIFTEE818 pKa = 4.12SDD820 pKa = 3.13GGSGIFVNLALIVAQDD836 pKa = 3.76GEE838 pKa = 4.57PVHH841 pKa = 6.32VASAPLGTRR850 pKa = 11.84VEE852 pKa = 4.2VQEE855 pKa = 5.6LSIADD860 pKa = 3.68NQIALTMLALGPDD873 pKa = 4.22DD874 pKa = 5.75PICCPSQRR882 pKa = 11.84VSHH885 pKa = 6.5AYY887 pKa = 9.5AWEE890 pKa = 4.52GDD892 pKa = 3.69VLALVEE898 pKa = 4.46EE899 pKa = 4.69RR900 pKa = 11.84VEE902 pKa = 4.28TTPEE906 pKa = 3.53ATLPLSNTSWAWVEE920 pKa = 4.28TVFNDD925 pKa = 3.75GTRR928 pKa = 11.84ITPATEE934 pKa = 4.09GAFTLTFTPEE944 pKa = 4.31GEE946 pKa = 4.34VSATTDD952 pKa = 3.45CNTFGGSYY960 pKa = 9.43RR961 pKa = 11.84AEE963 pKa = 4.18AGEE966 pKa = 3.9LAIEE970 pKa = 4.58FLTSTTIACPEE981 pKa = 4.09DD982 pKa = 3.51AQEE985 pKa = 4.16QEE987 pKa = 4.9FIADD991 pKa = 3.27VAAANGYY998 pKa = 6.87TVTEE1002 pKa = 4.33EE1003 pKa = 3.96GRR1005 pKa = 11.84LALLLPFNSGSVIFVPAAEE1024 pKa = 4.22VQVSDD1029 pKa = 3.96EE1030 pKa = 4.28AAAEE1034 pKa = 4.06ATTTDD1039 pKa = 3.35TAAEE1043 pKa = 4.34TTAEE1047 pKa = 4.32APLATTPVALPGTVWNWVRR1066 pKa = 11.84TQYY1069 pKa = 11.69SNGAIIAPSDD1079 pKa = 3.34PSLYY1083 pKa = 10.86VLTFGEE1089 pKa = 4.77DD1090 pKa = 3.18GTVNVQDD1097 pKa = 4.15DD1098 pKa = 4.57CNVVNGVFTAFADD1111 pKa = 3.68QALTLDD1117 pKa = 4.21LQTSTMAACAPDD1129 pKa = 3.38SLHH1132 pKa = 5.98YY1133 pKa = 10.56QFILDD1138 pKa = 3.94LSAAASYY1145 pKa = 9.01FFQDD1149 pKa = 2.42GRR1151 pKa = 11.84LFIDD1155 pKa = 4.15LKK1157 pKa = 10.93FDD1159 pKa = 3.24TGVIEE1164 pKa = 5.11FALADD1169 pKa = 3.53

Molecular weight: 124.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I0HZV6|I0HZV6_CALAS ATP synthase epsilon chain OS=Caldilinea aerophila (strain DSM 14535 / JCM 11387 / NBRC 104270 / STL-6-O1) OX=926550 GN=atpC PE=3 SV=1
MM1 pKa = 6.75ATKK4 pKa = 9.53RR5 pKa = 11.84TWQPKK10 pKa = 6.57VRR12 pKa = 11.84KK13 pKa = 9.2RR14 pKa = 11.84LKK16 pKa = 8.26THH18 pKa = 5.87GFRR21 pKa = 11.84IRR23 pKa = 11.84MSTPGGRR30 pKa = 11.84KK31 pKa = 7.15VLKK34 pKa = 10.05RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84LKK39 pKa = 10.44GRR41 pKa = 11.84ARR43 pKa = 11.84LTVQLSHH50 pKa = 7.07RR51 pKa = 11.84KK52 pKa = 9.64AII54 pKa = 3.9

Molecular weight: 6.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4097

0

4097

1491047

37

5788

363.9

40.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.222 ± 0.05

0.832 ± 0.013

4.876 ± 0.028

6.206 ± 0.04

3.761 ± 0.029

7.725 ± 0.035

2.127 ± 0.021

5.351 ± 0.029

2.399 ± 0.027

10.988 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.245 ± 0.018

2.889 ± 0.024

5.755 ± 0.036

3.856 ± 0.024

7.023 ± 0.039

4.936 ± 0.025

5.498 ± 0.048

7.66 ± 0.031

1.76 ± 0.021

2.891 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski