Sphingomonas sp. Leaf42

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3352 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q4LVF3|A0A0Q4LVF3_9SPHN Uncharacterized protein OS=Sphingomonas sp. Leaf42 OX=1736219 GN=ASE97_10910 PE=4 SV=1
MM1 pKa = 7.23RR2 pKa = 11.84TVSEE6 pKa = 4.18DD7 pKa = 3.16AAVRR11 pKa = 11.84LYY13 pKa = 11.0HH14 pKa = 7.06LDD16 pKa = 3.76DD17 pKa = 4.44EE18 pKa = 5.43GGVATTILYY27 pKa = 7.66GTLSEE32 pKa = 4.62ALAAAANEE40 pKa = 4.11PEE42 pKa = 4.09AVQGGLYY49 pKa = 10.13LQTSSDD55 pKa = 3.49VVAYY59 pKa = 10.77LDD61 pKa = 4.99LIEE64 pKa = 4.37GG65 pKa = 3.86

Molecular weight: 6.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q4LL89|A0A0Q4LL89_9SPHN Uncharacterized protein OS=Sphingomonas sp. Leaf42 OX=1736219 GN=ASE97_15040 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.97KK41 pKa = 10.66LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3352

0

3352

1119172

29

5377

333.9

35.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.299 ± 0.066

0.678 ± 0.011

6.23 ± 0.034

4.584 ± 0.038

3.383 ± 0.029

9.411 ± 0.084

1.899 ± 0.022

4.722 ± 0.027

2.333 ± 0.029

9.82 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.228 ± 0.022

2.345 ± 0.038

5.493 ± 0.039

2.933 ± 0.023

7.934 ± 0.052

4.648 ± 0.034

5.717 ± 0.04

7.707 ± 0.036

1.459 ± 0.02

2.175 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski