Amino acid dipepetide frequency for Sphingomonas sp. Leaf42

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.002AlaAla: 22.002 ± 0.269
1.026AlaCys: 1.026 ± 0.033
8.523AlaAsp: 8.523 ± 0.116
6.972AlaGlu: 6.972 ± 0.118
4.396AlaPhe: 4.396 ± 0.069
13.134AlaGly: 13.134 ± 0.168
2.422AlaHis: 2.422 ± 0.05
7.529AlaIle: 7.529 ± 0.095
3.72AlaLys: 3.72 ± 0.081
14.639AlaLeu: 14.639 ± 0.185
4.087AlaMet: 4.087 ± 0.072
3.164AlaAsn: 3.164 ± 0.068
6.849AlaPro: 6.849 ± 0.113
4.739AlaGln: 4.739 ± 0.086
11.098AlaArg: 11.098 ± 0.137
6.023AlaSer: 6.023 ± 0.076
8.549AlaThr: 8.549 ± 0.108
9.714AlaVal: 9.714 ± 0.089
1.778AlaTrp: 1.778 ± 0.04
2.626AlaTyr: 2.626 ± 0.049
0.002AlaXaa: 0.002 ± 0.001
Cys
0.877CysAla: 0.877 ± 0.028
0.072CysCys: 0.072 ± 0.008
0.48CysAsp: 0.48 ± 0.022
0.306CysGlu: 0.306 ± 0.018
0.243CysPhe: 0.243 ± 0.017
0.776CysGly: 0.776 ± 0.028
0.185CysHis: 0.185 ± 0.013
0.298CysIle: 0.298 ± 0.017
0.114CysLys: 0.114 ± 0.01
0.582CysLeu: 0.582 ± 0.02
0.119CysMet: 0.119 ± 0.009
0.153CysAsn: 0.153 ± 0.012
0.375CysPro: 0.375 ± 0.017
0.15CysGln: 0.15 ± 0.012
0.521CysArg: 0.521 ± 0.02
0.369CysSer: 0.369 ± 0.021
0.376CysThr: 0.376 ± 0.02
0.538CysVal: 0.538 ± 0.023
0.09CysTrp: 0.09 ± 0.009
0.154CysTyr: 0.154 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
8.906AspAla: 8.906 ± 0.102
0.406AspCys: 0.406 ± 0.02
3.754AspAsp: 3.754 ± 0.061
2.849AspGlu: 2.849 ± 0.055
2.003AspPhe: 2.003 ± 0.044
6.269AspGly: 6.269 ± 0.082
1.354AspHis: 1.354 ± 0.04
2.474AspIle: 2.474 ± 0.055
1.382AspLys: 1.382 ± 0.038
5.945AspLeu: 5.945 ± 0.075
1.257AspMet: 1.257 ± 0.037
1.195AspAsn: 1.195 ± 0.041
4.24AspPro: 4.24 ± 0.064
1.696AspGln: 1.696 ± 0.038
5.931AspArg: 5.931 ± 0.086
2.263AspSer: 2.263 ± 0.042
3.158AspThr: 3.158 ± 0.055
4.42AspVal: 4.42 ± 0.069
1.231AspTrp: 1.231 ± 0.035
1.561AspTyr: 1.561 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
6.907GluAla: 6.907 ± 0.104
0.224GluCys: 0.224 ± 0.015
2.107GluAsp: 2.107 ± 0.052
2.056GluGlu: 2.056 ± 0.057
1.171GluPhe: 1.171 ± 0.036
4.003GluGly: 4.003 ± 0.064
0.944GluHis: 0.944 ± 0.027
2.182GluIle: 2.182 ± 0.057
1.242GluLys: 1.242 ± 0.043
4.338GluLeu: 4.338 ± 0.069
1.115GluMet: 1.115 ± 0.035
1.006GluAsn: 1.006 ± 0.032
2.343GluPro: 2.343 ± 0.054
1.709GluGln: 1.709 ± 0.046
5.046GluArg: 5.046 ± 0.085
1.806GluSer: 1.806 ± 0.049
2.76GluThr: 2.76 ± 0.05
3.299GluVal: 3.299 ± 0.063
0.724GluTrp: 0.724 ± 0.026
0.861GluTyr: 0.861 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
5.229PheAla: 5.229 ± 0.077
0.258PheCys: 0.258 ± 0.013
2.734PheAsp: 2.734 ± 0.048
1.635PheGlu: 1.635 ± 0.042
1.158PhePhe: 1.158 ± 0.037
3.649PheGly: 3.649 ± 0.072
0.674PheHis: 0.674 ± 0.023
1.029PheIle: 1.029 ± 0.033
0.646PheLys: 0.646 ± 0.028
3.003PheLeu: 3.003 ± 0.055
0.605PheMet: 0.605 ± 0.024
0.951PheAsn: 0.951 ± 0.037
1.415PhePro: 1.415 ± 0.037
0.845PheGln: 0.845 ± 0.029
2.218PheArg: 2.218 ± 0.05
1.638PheSer: 1.638 ± 0.044
1.968PheThr: 1.968 ± 0.041
2.845PheVal: 2.845 ± 0.05
0.466PheTrp: 0.466 ± 0.024
0.862PheTyr: 0.862 ± 0.033
0.0PheXaa: 0.0 ± 0.0
Gly
10.594GlyAla: 10.594 ± 0.148
0.796GlyCys: 0.796 ± 0.031
5.833GlyAsp: 5.833 ± 0.095
4.777GlyGlu: 4.777 ± 0.068
3.81GlyPhe: 3.81 ± 0.064
9.537GlyGly: 9.537 ± 0.519
1.892GlyHis: 1.892 ± 0.042
4.731GlyIle: 4.731 ± 0.082
2.656GlyLys: 2.656 ± 0.052
8.454GlyLeu: 8.454 ± 0.103
2.413GlyMet: 2.413 ± 0.051
2.346GlyAsn: 2.346 ± 0.08
3.802GlyPro: 3.802 ± 0.07
3.185GlyGln: 3.185 ± 0.061
7.345GlyArg: 7.345 ± 0.102
4.846GlySer: 4.846 ± 0.122
5.57GlyThr: 5.57 ± 0.096
7.158GlyVal: 7.158 ± 0.09
1.861GlyTrp: 1.861 ± 0.047
2.501GlyTyr: 2.501 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
2.709HisAla: 2.709 ± 0.057
0.162HisCys: 0.162 ± 0.013
1.327HisAsp: 1.327 ± 0.038
0.804HisGlu: 0.804 ± 0.031
0.675HisPhe: 0.675 ± 0.024
2.145HisGly: 2.145 ± 0.047
0.566HisHis: 0.566 ± 0.025
0.77HisIle: 0.77 ± 0.029
0.314HisLys: 0.314 ± 0.018
1.843HisLeu: 1.843 ± 0.041
0.345HisMet: 0.345 ± 0.019
0.404HisAsn: 0.404 ± 0.019
1.215HisPro: 1.215 ± 0.036
0.44HisGln: 0.44 ± 0.02
1.513HisArg: 1.513 ± 0.041
0.8HisSer: 0.8 ± 0.025
0.64HisThr: 0.64 ± 0.025
1.48HisVal: 1.48 ± 0.038
0.369HisTrp: 0.369 ± 0.02
0.474HisTyr: 0.474 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
8.397IleAla: 8.397 ± 0.093
0.326IleCys: 0.326 ± 0.018
4.368IleAsp: 4.368 ± 0.057
3.203IleGlu: 3.203 ± 0.064
1.191IlePhe: 1.191 ± 0.032
5.412IleGly: 5.412 ± 0.077
0.791IleHis: 0.791 ± 0.029
1.409IleIle: 1.409 ± 0.042
0.842IleLys: 0.842 ± 0.031
3.696IleLeu: 3.696 ± 0.064
0.616IleMet: 0.616 ± 0.024
1.062IleAsn: 1.062 ± 0.038
1.977IlePro: 1.977 ± 0.043
0.916IleGln: 0.916 ± 0.03
3.098IleArg: 3.098 ± 0.054
1.678IleSer: 1.678 ± 0.045
2.417IleThr: 2.417 ± 0.054
4.609IleVal: 4.609 ± 0.076
0.453IleTrp: 0.453 ± 0.023
0.754IleTyr: 0.754 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
3.32LysAla: 3.32 ± 0.07
0.1LysCys: 0.1 ± 0.01
1.192LysAsp: 1.192 ± 0.038
0.872LysGlu: 0.872 ± 0.028
0.667LysPhe: 0.667 ± 0.03
2.222LysGly: 2.222 ± 0.047
0.38LysHis: 0.38 ± 0.018
0.958LysIle: 0.958 ± 0.034
0.693LysLys: 0.693 ± 0.033
2.638LysLeu: 2.638 ± 0.061
0.541LysMet: 0.541 ± 0.026
0.525LysAsn: 0.525 ± 0.023
1.615LysPro: 1.615 ± 0.049
0.673LysGln: 0.673 ± 0.027
1.959LysArg: 1.959 ± 0.047
1.118LysSer: 1.118 ± 0.039
1.306LysThr: 1.306 ± 0.036
1.814LysVal: 1.814 ± 0.052
0.308LysTrp: 0.308 ± 0.016
0.432LysTyr: 0.432 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
14.841LeuAla: 14.841 ± 0.173
0.693LeuCys: 0.693 ± 0.025
6.277LeuAsp: 6.277 ± 0.085
3.663LeuGlu: 3.663 ± 0.075
3.487LeuPhe: 3.487 ± 0.055
8.474LeuGly: 8.474 ± 0.097
1.83LeuHis: 1.83 ± 0.048
4.396LeuIle: 4.396 ± 0.068
2.379LeuLys: 2.379 ± 0.06
9.878LeuLeu: 9.878 ± 0.149
1.868LeuMet: 1.868 ± 0.047
2.358LeuAsn: 2.358 ± 0.053
6.173LeuPro: 6.173 ± 0.073
2.22LeuGln: 2.22 ± 0.049
7.253LeuArg: 7.253 ± 0.102
5.636LeuSer: 5.636 ± 0.068
6.071LeuThr: 6.071 ± 0.083
7.447LeuVal: 7.447 ± 0.095
1.304LeuTrp: 1.304 ± 0.042
1.957LeuTyr: 1.957 ± 0.044
0.0LeuXaa: 0.0 ± 0.0
Met
3.318MetAla: 3.318 ± 0.055
0.118MetCys: 0.118 ± 0.011
0.944MetAsp: 0.944 ± 0.03
0.796MetGlu: 0.796 ± 0.03
0.616MetPhe: 0.616 ± 0.024
1.746MetGly: 1.746 ± 0.046
0.331MetHis: 0.331 ± 0.018
1.223MetIle: 1.223 ± 0.037
0.696MetLys: 0.696 ± 0.026
2.586MetLeu: 2.586 ± 0.054
0.541MetMet: 0.541 ± 0.026
0.571MetAsn: 0.571 ± 0.025
1.363MetPro: 1.363 ± 0.04
0.662MetGln: 0.662 ± 0.027
1.774MetArg: 1.774 ± 0.043
1.099MetSer: 1.099 ± 0.03
1.714MetThr: 1.714 ± 0.041
1.707MetVal: 1.707 ± 0.042
0.228MetTrp: 0.228 ± 0.015
0.248MetTyr: 0.248 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
3.05AsnAla: 3.05 ± 0.062
0.195AsnCys: 0.195 ± 0.014
1.387AsnAsp: 1.387 ± 0.039
0.92AsnGlu: 0.92 ± 0.033
0.852AsnPhe: 0.852 ± 0.037
2.517AsnGly: 2.517 ± 0.078
0.466AsnHis: 0.466 ± 0.02
1.114AsnIle: 1.114 ± 0.04
0.46AsnLys: 0.46 ± 0.02
2.289AsnLeu: 2.289 ± 0.054
0.437AsnMet: 0.437 ± 0.021
0.672AsnAsn: 0.672 ± 0.038
1.625AsnPro: 1.625 ± 0.051
0.644AsnGln: 0.644 ± 0.024
1.84AsnArg: 1.84 ± 0.043
1.024AsnSer: 1.024 ± 0.037
1.134AsnThr: 1.134 ± 0.048
1.821AsnVal: 1.821 ± 0.06
0.36AsnTrp: 0.36 ± 0.017
0.645AsnTyr: 0.645 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
8.331ProAla: 8.331 ± 0.116
0.306ProCys: 0.306 ± 0.017
3.892ProAsp: 3.892 ± 0.061
2.765ProGlu: 2.765 ± 0.052
1.917ProPhe: 1.917 ± 0.045
5.343ProGly: 5.343 ± 0.065
1.03ProHis: 1.03 ± 0.035
2.335ProIle: 2.335 ± 0.048
1.283ProLys: 1.283 ± 0.042
5.075ProLeu: 5.075 ± 0.08
1.163ProMet: 1.163 ± 0.03
1.217ProAsn: 1.217 ± 0.039
3.096ProPro: 3.096 ± 0.074
1.636ProGln: 1.636 ± 0.035
3.373ProArg: 3.373 ± 0.061
2.354ProSer: 2.354 ± 0.054
2.876ProThr: 2.876 ± 0.06
5.047ProVal: 5.047 ± 0.075
0.783ProTrp: 0.783 ± 0.024
1.109ProTyr: 1.109 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
4.417GlnAla: 4.417 ± 0.074
0.18GlnCys: 0.18 ± 0.012
1.272GlnAsp: 1.272 ± 0.031
0.951GlnGlu: 0.951 ± 0.03
1.017GlnPhe: 1.017 ± 0.038
2.622GlnGly: 2.622 ± 0.05
0.511GlnHis: 0.511 ± 0.026
1.399GlnIle: 1.399 ± 0.036
0.623GlnLys: 0.623 ± 0.026
2.74GlnLeu: 2.74 ± 0.057
0.696GlnMet: 0.696 ± 0.024
0.674GlnAsn: 0.674 ± 0.027
1.782GlnPro: 1.782 ± 0.043
1.107GlnGln: 1.107 ± 0.037
2.612GlnArg: 2.612 ± 0.053
1.551GlnSer: 1.551 ± 0.069
1.658GlnThr: 1.658 ± 0.045
2.496GlnVal: 2.496 ± 0.054
0.408GlnTrp: 0.408 ± 0.018
0.617GlnTyr: 0.617 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
10.275ArgAla: 10.275 ± 0.132
0.476ArgCys: 0.476 ± 0.022
4.976ArgAsp: 4.976 ± 0.079
3.616ArgGlu: 3.616 ± 0.067
3.457ArgPhe: 3.457 ± 0.057
5.798ArgGly: 5.798 ± 0.079
1.726ArgHis: 1.726 ± 0.044
4.644ArgIle: 4.644 ± 0.068
1.545ArgLys: 1.545 ± 0.041
8.427ArgLeu: 8.427 ± 0.106
2.131ArgMet: 2.131 ± 0.045
1.678ArgAsn: 1.678 ± 0.042
4.164ArgPro: 4.164 ± 0.074
2.488ArgGln: 2.488 ± 0.05
6.588ArgArg: 6.588 ± 0.106
3.839ArgSer: 3.839 ± 0.057
4.273ArgThr: 4.273 ± 0.06
5.606ArgVal: 5.606 ± 0.073
1.421ArgTrp: 1.421 ± 0.046
2.211ArgTyr: 2.211 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
5.956SerAla: 5.956 ± 0.076
0.315SerCys: 0.315 ± 0.02
2.948SerAsp: 2.948 ± 0.055
1.948SerGlu: 1.948 ± 0.044
1.924SerPhe: 1.924 ± 0.045
5.233SerGly: 5.233 ± 0.112
0.792SerHis: 0.792 ± 0.03
2.494SerIle: 2.494 ± 0.066
1.033SerLys: 1.033 ± 0.033
4.446SerLeu: 4.446 ± 0.071
0.926SerMet: 0.926 ± 0.032
1.179SerAsn: 1.179 ± 0.04
2.704SerPro: 2.704 ± 0.055
1.204SerGln: 1.204 ± 0.036
3.162SerArg: 3.162 ± 0.051
2.146SerSer: 2.146 ± 0.052
2.457SerThr: 2.457 ± 0.055
3.746SerVal: 3.746 ± 0.072
0.663SerTrp: 0.663 ± 0.025
1.204SerTyr: 1.204 ± 0.04
0.0SerXaa: 0.0 ± 0.0
Thr
7.303ThrAla: 7.303 ± 0.088
0.337ThrCys: 0.337 ± 0.019
3.155ThrAsp: 3.155 ± 0.053
1.985ThrGlu: 1.985 ± 0.045
1.728ThrPhe: 1.728 ± 0.038
5.956ThrGly: 5.956 ± 0.106
0.969ThrHis: 0.969 ± 0.033
3.467ThrIle: 3.467 ± 0.075
1.156ThrLys: 1.156 ± 0.032
6.759ThrLeu: 6.759 ± 0.085
1.223ThrMet: 1.223 ± 0.036
1.341ThrAsn: 1.341 ± 0.045
4.044ThrPro: 4.044 ± 0.072
1.579ThrGln: 1.579 ± 0.046
4.128ThrArg: 4.128 ± 0.072
2.502ThrSer: 2.502 ± 0.064
3.323ThrThr: 3.323 ± 0.078
4.426ThrVal: 4.426 ± 0.067
0.614ThrTrp: 0.614 ± 0.022
1.173ThrTyr: 1.173 ± 0.038
0.0ThrXaa: 0.0 ± 0.0
Val
12.153ValAla: 12.153 ± 0.147
0.48ValCys: 0.48 ± 0.023
4.677ValAsp: 4.677 ± 0.063
4.298ValGlu: 4.298 ± 0.074
2.128ValPhe: 2.128 ± 0.05
6.088ValGly: 6.088 ± 0.092
1.271ValHis: 1.271 ± 0.036
3.366ValIle: 3.366 ± 0.068
1.742ValLys: 1.742 ± 0.048
7.129ValLeu: 7.129 ± 0.091
1.555ValMet: 1.555 ± 0.035
1.898ValAsn: 1.898 ± 0.049
4.471ValPro: 4.471 ± 0.068
2.169ValGln: 2.169 ± 0.046
6.114ValArg: 6.114 ± 0.081
3.823ValSer: 3.823 ± 0.061
5.01ValThr: 5.01 ± 0.087
6.475ValVal: 6.475 ± 0.093
0.833ValTrp: 0.833 ± 0.028
1.395ValTyr: 1.395 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.513TrpAla: 1.513 ± 0.042
0.12TrpCys: 0.12 ± 0.011
0.773TrpAsp: 0.773 ± 0.03
0.509TrpGlu: 0.509 ± 0.023
0.584TrpPhe: 0.584 ± 0.022
1.023TrpGly: 1.023 ± 0.031
0.361TrpHis: 0.361 ± 0.019
0.694TrpIle: 0.694 ± 0.026
0.353TrpLys: 0.353 ± 0.018
1.68TrpLeu: 1.68 ± 0.045
0.33TrpMet: 0.33 ± 0.017
0.44TrpAsn: 0.44 ± 0.021
0.697TrpPro: 0.697 ± 0.027
0.619TrpGln: 0.619 ± 0.026
1.577TrpArg: 1.577 ± 0.043
0.907TrpSer: 0.907 ± 0.032
0.889TrpThr: 0.889 ± 0.028
0.856TrpVal: 0.856 ± 0.035
0.335TrpTrp: 0.335 ± 0.021
0.329TrpTyr: 0.329 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.895TyrAla: 2.895 ± 0.054
0.186TyrCys: 0.186 ± 0.014
1.677TyrAsp: 1.677 ± 0.056
0.919TyrGlu: 0.919 ± 0.031
0.801TyrPhe: 0.801 ± 0.027
2.16TyrGly: 2.16 ± 0.046
0.497TyrHis: 0.497 ± 0.025
0.704TyrIle: 0.704 ± 0.026
0.454TyrLys: 0.454 ± 0.022
2.056TyrLeu: 2.056 ± 0.046
0.311TyrMet: 0.311 ± 0.016
0.621TyrAsn: 0.621 ± 0.031
0.984TyrPro: 0.984 ± 0.03
0.652TyrGln: 0.652 ± 0.023
2.19TyrArg: 2.19 ± 0.05
1.057TyrSer: 1.057 ± 0.034
1.02TyrThr: 1.02 ± 0.038
1.571TyrVal: 1.571 ± 0.039
0.36TyrTrp: 0.36 ± 0.018
0.641TyrTyr: 0.641 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.001XaaHis: 0.001 ± 0.001
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.001XaaLeu: 0.001 ± 0.001
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3352 proteins (1119172 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski