Dendrosporobacter quercicolus
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3747 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G9KEA8|A0A1G9KEA8_9FIRM Uncharacterized protein OS=Dendrosporobacter quercicolus OX=146817 GN=SAMN04488502_1017 PE=4 SV=1
AA1 pKa = 8.64 IGEE4 pKa = 4.34 TGVTGEE10 pKa = 4.34 TGATGEE16 pKa = 4.28 TGATGEE22 pKa = 4.38 TGATGAIGEE31 pKa = 4.42 TGATGVTGASADD43 pKa = 3.93 TNVCLMTSSLGNINTTITPDD63 pKa = 3.07 EE64 pKa = 4.63 GGGNNQAIGGVIFGGDD80 pKa = 3.29 MDD82 pKa = 4.4 RR83 pKa = 11.84 VVSHH87 pKa = 6.68 AAAYY91 pKa = 8.73 VIQVGAGTGQFQIAVLQATSNSTAQVIGITAIATTISGGLEE132 pKa = 4.16 VLPLIAPVTLLGNQLYY148 pKa = 10.32 YY149 pKa = 10.8 LAVYY153 pKa = 9.46 NQVNASQLGAVVAGTNTVADD173 pKa = 4.02 APPINFRR180 pKa = 11.84 EE181 pKa = 4.16 QNLSGFTIGQTISTSDD197 pKa = 3.32 VNLWKK202 pKa = 9.5 TPWLAVLEE210 pKa = 4.37
Molecular weight: 20.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.77
IPC2_protein 3.846
IPC_protein 3.732
Toseland 3.567
ProMoST 3.859
Dawson 3.719
Bjellqvist 3.961
Wikipedia 3.63
Rodwell 3.579
Grimsley 3.478
Solomon 3.681
Lehninger 3.643
Nozaki 3.846
DTASelect 3.973
Thurlkill 3.617
EMBOSS 3.643
Sillero 3.859
Patrickios 0.693
IPC_peptide 3.694
IPC2_peptide 3.834
IPC2.peptide.svr19 3.815
Protein with the highest isoelectric point:
>tr|A0A1G9LVG1|A0A1G9LVG1_9FIRM Integrase core domain-containing protein OS=Dendrosporobacter quercicolus OX=146817 GN=SAMN04488502_101481 PE=4 SV=1
MM1 pKa = 6.96 TKK3 pKa = 9.99 YY4 pKa = 10.43 RR5 pKa = 11.84 EE6 pKa = 4.04 ILRR9 pKa = 11.84 LQSLGFSQQNIAYY22 pKa = 9.25 SSNVSKK28 pKa = 9.83 KK29 pKa = 7.9 TVNRR33 pKa = 11.84 VINRR37 pKa = 11.84 ARR39 pKa = 11.84 EE40 pKa = 4.05 LNISWPLADD49 pKa = 5.32 RR50 pKa = 11.84 LTLYY54 pKa = 8.66 WSKK57 pKa = 11.13 YY58 pKa = 8.11 CSPTIRR64 pKa = 11.84 RR65 pKa = 11.84 VKK67 pKa = 10.5 SRR69 pKa = 11.84 EE70 pKa = 4.05 CQTLPTSARR79 pKa = 11.84 NCC81 pKa = 3.73
Molecular weight: 9.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.838
IPC_protein 10.643
Toseland 10.613
ProMoST 10.35
Dawson 10.745
Bjellqvist 10.482
Wikipedia 10.965
Rodwell 10.979
Grimsley 10.818
Solomon 10.847
Lehninger 10.818
Nozaki 10.628
DTASelect 10.482
Thurlkill 10.643
EMBOSS 11.023
Sillero 10.687
Patrickios 10.716
IPC_peptide 10.847
IPC2_peptide 9.692
IPC2.peptide.svr19 8.321
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3747
0
3747
1190279
39
8085
317.7
34.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.982 ± 0.067
1.17 ± 0.02
4.823 ± 0.027
6.006 ± 0.043
3.831 ± 0.032
7.76 ± 0.057
1.85 ± 0.018
7.067 ± 0.037
5.261 ± 0.041
10.321 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.502 ± 0.023
3.845 ± 0.033
4.023 ± 0.034
4.174 ± 0.034
5.113 ± 0.04
5.494 ± 0.037
5.293 ± 0.043
7.296 ± 0.035
0.972 ± 0.015
3.216 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here