Ascidiaceihabitans donghaensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Ascidiaceihabitans

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4027 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R8BFQ3|A0A2R8BFQ3_9RHOB Uncharacterized protein OS=Ascidiaceihabitans donghaensis OX=1510460 GN=ASD8599_02589 PE=4 SV=1
MM1 pKa = 7.48GKK3 pKa = 9.7FLAVLLVGSAFGAGIFMYY21 pKa = 10.1YY22 pKa = 9.36LQVYY26 pKa = 8.3GFYY29 pKa = 10.92DD30 pKa = 3.79EE31 pKa = 5.55VVGGDD36 pKa = 3.51VQLVSVVDD44 pKa = 4.57DD45 pKa = 4.06LPEE48 pKa = 4.52TISFDD53 pKa = 3.25NLQAIDD59 pKa = 4.4ADD61 pKa = 4.25SSPLRR66 pKa = 11.84YY67 pKa = 9.08RR68 pKa = 11.84ACFTTDD74 pKa = 3.18LSLSLLSEE82 pKa = 4.49TYY84 pKa = 10.72VGLEE88 pKa = 3.74TATPRR93 pKa = 11.84NAPEE97 pKa = 3.81WFDD100 pKa = 4.19CFDD103 pKa = 3.82AATIAAEE110 pKa = 4.32LEE112 pKa = 4.23AGTAIPFLGGKK123 pKa = 8.5NVEE126 pKa = 4.41FGVDD130 pKa = 3.57RR131 pKa = 11.84IVAITEE137 pKa = 3.95DD138 pKa = 3.03GRR140 pKa = 11.84GYY142 pKa = 10.46VWHH145 pKa = 7.71DD146 pKa = 4.17LNDD149 pKa = 4.55CGDD152 pKa = 3.41KK153 pKa = 10.87AYY155 pKa = 10.72DD156 pKa = 3.5GTIVGEE162 pKa = 4.01EE163 pKa = 4.14CPRR166 pKa = 11.84RR167 pKa = 11.84EE168 pKa = 4.07TEE170 pKa = 3.69

Molecular weight: 18.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R8BFM7|A0A2R8BFM7_9RHOB NHase_beta domain-containing protein OS=Ascidiaceihabitans donghaensis OX=1510460 GN=ASD8599_02665 PE=4 SV=1
MM1 pKa = 7.68PLLRR5 pKa = 11.84INATSEE11 pKa = 4.17GPQLHH16 pKa = 6.83GSPVPARR23 pKa = 11.84RR24 pKa = 11.84VIQTAARR31 pKa = 11.84SKK33 pKa = 10.29GPVIIMVHH41 pKa = 5.19GFKK44 pKa = 10.49YY45 pKa = 10.68DD46 pKa = 3.38PEE48 pKa = 4.44TPRR51 pKa = 11.84FCPHH55 pKa = 6.53ISIFDD60 pKa = 3.7APSGRR65 pKa = 11.84KK66 pKa = 8.84RR67 pKa = 11.84DD68 pKa = 3.42SWPAALGFAGRR79 pKa = 11.84DD80 pKa = 3.75PNEE83 pKa = 3.91GLAIAFAWRR92 pKa = 11.84ARR94 pKa = 11.84GTLWHH99 pKa = 7.12ALRR102 pKa = 11.84TAAQAGQNLAQVIAIVKK119 pKa = 7.94RR120 pKa = 11.84TAPARR125 pKa = 11.84AVHH128 pKa = 6.15VICHH132 pKa = 5.32SMGSEE137 pKa = 3.99VLFEE141 pKa = 4.71ALHH144 pKa = 6.15HH145 pKa = 6.41LPAHH149 pKa = 6.55SIQRR153 pKa = 11.84AIILTAASYY162 pKa = 10.28QSRR165 pKa = 11.84ASAALHH171 pKa = 5.97TDD173 pKa = 3.24AGQTLEE179 pKa = 4.61LFNITSRR186 pKa = 11.84EE187 pKa = 3.63NDD189 pKa = 3.27LFDD192 pKa = 5.55AGFEE196 pKa = 4.23TLIKK200 pKa = 10.5AATPSDD206 pKa = 3.57RR207 pKa = 11.84AVGAGLCAPNAVTIEE222 pKa = 3.99IDD224 pKa = 4.3CDD226 pKa = 3.41LTLQRR231 pKa = 11.84LRR233 pKa = 11.84LTGADD238 pKa = 3.03IAPAARR244 pKa = 11.84RR245 pKa = 11.84ISHH248 pKa = 6.39WSAYY252 pKa = 7.31MRR254 pKa = 11.84PGLMPFYY261 pKa = 10.65NSLLRR266 pKa = 11.84RR267 pKa = 11.84PSEE270 pKa = 4.14TPLAALRR277 pKa = 11.84HH278 pKa = 5.54ALPLVGSQRR287 pKa = 11.84FARR290 pKa = 11.84LLPFMHH296 pKa = 6.95KK297 pKa = 9.95PQAGLPAPLL306 pKa = 4.38

Molecular weight: 33.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4027

0

4027

1246692

29

3150

309.6

33.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.687 ± 0.048

0.94 ± 0.012

6.522 ± 0.038

5.223 ± 0.039

3.921 ± 0.024

8.532 ± 0.043

2.175 ± 0.022

5.258 ± 0.026

3.682 ± 0.028

9.531 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.006 ± 0.022

3.034 ± 0.03

4.791 ± 0.025

3.527 ± 0.022

5.807 ± 0.039

5.376 ± 0.028

5.934 ± 0.041

7.383 ± 0.027

1.391 ± 0.018

2.281 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski