Dyadobacter jejuensis
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4730 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A316AQ66|A0A316AQ66_9BACT Tetratricopeptide repeat protein OS=Dyadobacter jejuensis OX=1082580 GN=CLV98_102106 PE=4 SV=1
MM1 pKa = 7.03 NQDD4 pKa = 2.86 NCFLLGYY11 pKa = 9.75 IVRR14 pKa = 11.84 THH16 pKa = 5.82 GMAGNLVFFLDD27 pKa = 3.51 VDD29 pKa = 4.12 DD30 pKa = 5.31 PSDD33 pKa = 4.16 YY34 pKa = 11.37 EE35 pKa = 4.18 DD36 pKa = 4.73 LEE38 pKa = 4.84 TVFVEE43 pKa = 4.85 LKK45 pKa = 10.93 GEE47 pKa = 4.03 LVPYY51 pKa = 10.25 FIEE54 pKa = 5.11 DD55 pKa = 3.83 LNLQKK60 pKa = 10.57 QSNAIVTFEE69 pKa = 4.71 DD70 pKa = 3.11 VDD72 pKa = 4.37 TIEE75 pKa = 4.48 KK76 pKa = 10.29 AQALVGASLYY86 pKa = 11.04 LPIDD90 pKa = 3.81 HH91 pKa = 6.7 LQSLDD96 pKa = 3.69 DD97 pKa = 3.89 DD98 pKa = 3.5 QFYY101 pKa = 10.01 YY102 pKa = 10.91 HH103 pKa = 7.45 EE104 pKa = 4.38 IKK106 pKa = 10.55 GYY108 pKa = 8.7 QVSDD112 pKa = 3.63 QEE114 pKa = 4.4 LGALGVVEE122 pKa = 5.02 EE123 pKa = 4.79 VYY125 pKa = 11.06 SLNGQDD131 pKa = 5.47 LINMKK136 pKa = 10.62 YY137 pKa = 10.15 KK138 pKa = 10.38 GAEE141 pKa = 3.95 VLIPTANDD149 pKa = 2.88 IVLKK153 pKa = 10.45 ADD155 pKa = 3.49 KK156 pKa = 10.05 SQKK159 pKa = 9.95 ILFVNLPEE167 pKa = 4.75 GLLDD171 pKa = 3.66 VYY173 pKa = 11.32 LDD175 pKa = 3.83 TEE177 pKa = 4.49 SEE179 pKa = 4.27 NIADD183 pKa = 4.92 DD184 pKa = 4.04 ADD186 pKa = 3.42
Molecular weight: 20.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.795
IPC_protein 3.795
Toseland 3.579
ProMoST 3.923
Dawson 3.795
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.63
Grimsley 3.49
Solomon 3.783
Lehninger 3.745
Nozaki 3.91
DTASelect 4.139
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.923
Patrickios 1.875
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.839
Protein with the highest isoelectric point:
>tr|A0A316API2|A0A316API2_9BACT 23S rRNA (Cytosine1962-C5)-methyltransferase OS=Dyadobacter jejuensis OX=1082580 GN=CLV98_103417 PE=4 SV=1
MM1 pKa = 7.41 GLLSSFHH8 pKa = 6.77 CLGMCGPIALALPIHH23 pKa = 6.11 GQSQRR28 pKa = 11.84 RR29 pKa = 11.84 QLLGLFSYY37 pKa = 11.09 NFGRR41 pKa = 11.84 ALLYY45 pKa = 10.74 ACLGALTGTLASALSWIGIFRR66 pKa = 11.84 YY67 pKa = 10.13 LSITAGILMVAYY79 pKa = 8.83 VLWSTRR85 pKa = 11.84 FQASLPTPAPIQKK98 pKa = 10.06 LLSILKK104 pKa = 9.93 KK105 pKa = 10.97 AMGSMLQRR113 pKa = 11.84 KK114 pKa = 8.51 NPMAWFLLGSLNGLVPCGLVYY135 pKa = 10.5 LALMSSMATGGPLEE149 pKa = 4.69 GATFMLLFGIGTMPMMLAVGFFKK172 pKa = 11.07 GLIGPSLRR180 pKa = 11.84 TRR182 pKa = 11.84 MRR184 pKa = 11.84 RR185 pKa = 11.84 LVPVMLTIAGIWLIARR201 pKa = 11.84 GVLIQYY207 pKa = 7.57 PASKK211 pKa = 10.58 NVTLPICHH219 pKa = 6.46 SPATTLEE226 pKa = 4.18 EE227 pKa = 4.24 KK228 pKa = 10.77
Molecular weight: 24.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.853
IPC_protein 10.496
Toseland 10.452
ProMoST 10.233
Dawson 10.613
Bjellqvist 10.35
Wikipedia 10.818
Rodwell 10.921
Grimsley 10.687
Solomon 10.672
Lehninger 10.643
Nozaki 10.482
DTASelect 10.335
Thurlkill 10.482
EMBOSS 10.847
Sillero 10.555
Patrickios 10.628
IPC_peptide 10.672
IPC2_peptide 9.575
IPC2.peptide.svr19 8.365
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4730
0
4730
1682925
26
2639
355.8
39.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.419 ± 0.034
0.742 ± 0.011
5.392 ± 0.025
5.868 ± 0.035
4.659 ± 0.023
7.225 ± 0.033
1.976 ± 0.02
6.604 ± 0.029
6.093 ± 0.034
9.826 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.395 ± 0.018
4.945 ± 0.037
4.174 ± 0.021
4.144 ± 0.02
4.407 ± 0.022
6.632 ± 0.029
5.712 ± 0.036
6.381 ± 0.023
1.333 ± 0.012
4.072 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here