Podoviridae sp. ct2cs2
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2W5W6|A0A5Q2W5W6_9CAUD Tail fiber adhesin OS=Podoviridae sp. ct2cs2 OX=2656707 PE=4 SV=1
MM1 pKa = 7.41 QIEE4 pKa = 4.79 LDD6 pKa = 4.1 YY7 pKa = 10.65 ITEE10 pKa = 4.26 ALHH13 pKa = 7.47 AIDD16 pKa = 6.28 LNLEE20 pKa = 4.07 DD21 pKa = 4.29 VNRR24 pKa = 11.84 GITPSGYY31 pKa = 8.59 LTINQYY37 pKa = 11.07 LDD39 pKa = 3.22 DD40 pKa = 3.9 MRR42 pKa = 11.84 YY43 pKa = 9.04 QLSEE47 pKa = 4.38 LNTEE51 pKa = 4.24 ISNLEE56 pKa = 3.96 INN58 pKa = 4.0
Molecular weight: 6.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.785
IPC2_protein 3.872
IPC_protein 3.732
Toseland 3.554
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.579
Grimsley 3.478
Solomon 3.681
Lehninger 3.63
Nozaki 3.859
DTASelect 3.999
Thurlkill 3.63
EMBOSS 3.668
Sillero 3.846
Patrickios 0.604
IPC_peptide 3.681
IPC2_peptide 3.821
IPC2.peptide.svr19 3.766
Protein with the highest isoelectric point:
>tr|A0A5Q2W272|A0A5Q2W272_9CAUD Endolysin OS=Podoviridae sp. ct2cs2 OX=2656707 PE=3 SV=1
MM1 pKa = 6.83 THH3 pKa = 5.09 YY4 pKa = 10.54 QIRR7 pKa = 11.84 KK8 pKa = 4.79 QWRR11 pKa = 11.84 IKK13 pKa = 10.54 LHH15 pKa = 6.57 AKK17 pKa = 9.35 RR18 pKa = 11.84 CKK20 pKa = 10.91 DD21 pKa = 3.06 NDD23 pKa = 3.41 QSTEE27 pKa = 3.76 RR28 pKa = 11.84 YY29 pKa = 9.45 KK30 pKa = 10.78 RR31 pKa = 11.84 DD32 pKa = 3.13 AKK34 pKa = 10.9 VLNRR38 pKa = 11.84 CMSIYY43 pKa = 10.29 KK44 pKa = 9.94 IEE46 pKa = 4.23 GTRR49 pKa = 11.84 ATWGQQ54 pKa = 3.14
Molecular weight: 6.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.264
IPC2_protein 9.575
IPC_protein 9.78
Toseland 10.394
ProMoST 9.984
Dawson 10.526
Bjellqvist 10.175
Wikipedia 10.672
Rodwell 10.994
Grimsley 10.57
Solomon 10.57
Lehninger 10.555
Nozaki 10.394
DTASelect 10.16
Thurlkill 10.409
EMBOSS 10.774
Sillero 10.438
Patrickios 10.76
IPC_peptide 10.57
IPC2_peptide 9.048
IPC2.peptide.svr19 8.518
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
68
0
68
11624
30
785
170.9
18.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.379 ± 0.551
1.067 ± 0.169
5.136 ± 0.285
5.936 ± 0.606
3.734 ± 0.233
7.528 ± 0.623
1.394 ± 0.228
6.237 ± 0.253
6.005 ± 0.539
7.614 ± 0.414
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.392 ± 0.202
5.945 ± 0.39
3.484 ± 0.251
4.181 ± 0.35
4.069 ± 0.282
7.416 ± 0.55
8.173 ± 0.705
5.893 ± 0.262
1.316 ± 0.166
4.104 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here