Vibrio virus K139
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8W755|Q8W755_9CAUD Orf13 OS=Vibrio virus K139 OX=70734 GN=orf13 PE=4 SV=1
MM1 pKa = 7.33 AALYY5 pKa = 10.07 KK6 pKa = 10.17 IEE8 pKa = 4.59 SYY10 pKa = 10.99 SDD12 pKa = 3.04 EE13 pKa = 4.2 AARR16 pKa = 11.84 QIGGFITEE24 pKa = 3.94 HH25 pKa = 6.68 GGRR28 pKa = 11.84 CVVAGFAVITDD39 pKa = 3.76 HH40 pKa = 7.52 LFQQGDD46 pKa = 3.65 AFQVLPLVARR56 pKa = 11.84 TSDD59 pKa = 3.75 QLSEE63 pKa = 3.36 WDD65 pKa = 3.74 YY66 pKa = 11.41 QQFSYY71 pKa = 11.44
Molecular weight: 7.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.256
IPC2_protein 4.622
IPC_protein 4.418
Toseland 4.266
ProMoST 4.571
Dawson 4.393
Bjellqvist 4.546
Wikipedia 4.317
Rodwell 4.266
Grimsley 4.19
Solomon 4.38
Lehninger 4.342
Nozaki 4.52
DTASelect 4.698
Thurlkill 4.304
EMBOSS 4.329
Sillero 4.546
Patrickios 2.028
IPC_peptide 4.393
IPC2_peptide 4.533
IPC2.peptide.svr19 4.464
Protein with the highest isoelectric point:
>tr|Q8W747|Q8W747_9CAUD Orf21 OS=Vibrio virus K139 OX=70734 GN=orf21 PE=4 SV=1
MM1 pKa = 7.44 FEE3 pKa = 4.32 IKK5 pKa = 10.75 AEE7 pKa = 3.96 DD8 pKa = 3.47 RR9 pKa = 11.84 SYY11 pKa = 11.38 LRR13 pKa = 11.84 VMEE16 pKa = 3.87 QLEE19 pKa = 4.31 LLGLDD24 pKa = 3.01 RR25 pKa = 11.84 KK26 pKa = 8.46 TRR28 pKa = 11.84 DD29 pKa = 2.86 KK30 pKa = 9.91 MLRR33 pKa = 11.84 RR34 pKa = 11.84 IGAQIAKK41 pKa = 5.88 TTRR44 pKa = 11.84 KK45 pKa = 9.87 NIRR48 pKa = 11.84 AQRR51 pKa = 11.84 DD52 pKa = 3.4 PDD54 pKa = 3.34 GSAWAKK60 pKa = 9.84 RR61 pKa = 11.84 KK62 pKa = 9.77 RR63 pKa = 11.84 GRR65 pKa = 11.84 GKK67 pKa = 10.15 LLKK70 pKa = 10.86 GFTQKK75 pKa = 10.56 LKK77 pKa = 10.79 HH78 pKa = 5.53 FQRR81 pKa = 11.84 DD82 pKa = 3.38 NNRR85 pKa = 11.84 TLVVGWPSARR95 pKa = 11.84 GRR97 pKa = 11.84 VAYY100 pKa = 8.84 EE101 pKa = 3.64 HH102 pKa = 6.53 HH103 pKa = 6.93 HH104 pKa = 6.91 GIAQEE109 pKa = 4.31 SGLSARR115 pKa = 11.84 KK116 pKa = 9.03 RR117 pKa = 11.84 QAKK120 pKa = 7.72 QQNEE124 pKa = 3.87 PRR126 pKa = 11.84 KK127 pKa = 7.6 TDD129 pKa = 3.41 PATRR133 pKa = 11.84 EE134 pKa = 3.71 QAKK137 pKa = 9.85 RR138 pKa = 11.84 CAISITASSLRR149 pKa = 11.84 GDD151 pKa = 3.47 RR152 pKa = 11.84 KK153 pKa = 9.49 EE154 pKa = 4.03 AKK156 pKa = 9.6 SPLWHH161 pKa = 6.78 GYY163 pKa = 9.39 EE164 pKa = 4.1 KK165 pKa = 10.39 TT166 pKa = 3.49
Molecular weight: 19.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.299
IPC2_protein 9.838
IPC_protein 10.804
Toseland 11.169
ProMoST 11.125
Dawson 11.199
Bjellqvist 10.979
Wikipedia 11.477
Rodwell 11.403
Grimsley 11.228
Solomon 11.462
Lehninger 11.403
Nozaki 11.155
DTASelect 10.979
Thurlkill 11.155
EMBOSS 11.594
Sillero 11.155
Patrickios 11.111
IPC_peptide 11.462
IPC2_peptide 10.043
IPC2.peptide.svr19 8.775
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
44
0
44
10200
53
800
231.8
25.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.245 ± 0.503
1.147 ± 0.209
5.471 ± 0.293
7.147 ± 0.409
3.833 ± 0.216
6.088 ± 0.282
1.961 ± 0.175
6.118 ± 0.254
6.314 ± 0.329
8.588 ± 0.294
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.814 ± 0.219
4.353 ± 0.235
4.137 ± 0.251
5.088 ± 0.241
5.108 ± 0.322
6.471 ± 0.302
5.676 ± 0.271
6.01 ± 0.268
1.549 ± 0.133
2.882 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here