Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Sordariales; Sordariaceae; Sordaria; Sordaria macrospora

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9890 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F7WAU0|F7WAU0_SORMK WGS project CABT00000000 data contig 2.65 OS=Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell) OX=771870 GN=SMAC_08767 PE=4 SV=1
MM1 pKa = 7.7SSQSNDD7 pKa = 2.69GDD9 pKa = 3.54EE10 pKa = 5.11DD11 pKa = 5.2DD12 pKa = 3.43IAKK15 pKa = 10.44AIRR18 pKa = 11.84RR19 pKa = 11.84LITICNHH26 pKa = 5.66LFEE29 pKa = 6.96DD30 pKa = 4.75DD31 pKa = 4.4GSDD34 pKa = 4.2SAMDD38 pKa = 4.19DD39 pKa = 3.39NQYY42 pKa = 11.71DD43 pKa = 3.69NFIAALQPNEE53 pKa = 4.24TEE55 pKa = 4.18TEE57 pKa = 3.98PFSTHH62 pKa = 5.81EE63 pKa = 4.07EE64 pKa = 4.01LVNEE68 pKa = 4.14YY69 pKa = 10.62RR70 pKa = 11.84SFAQSVQTHH79 pKa = 4.69QDD81 pKa = 2.88VVNEE85 pKa = 3.82FRR87 pKa = 11.84SFSEE91 pKa = 4.2SIQTHH96 pKa = 4.83EE97 pKa = 4.07EE98 pKa = 4.1VVNEE102 pKa = 3.94FMSFVQWNYY111 pKa = 10.94ANYY114 pKa = 10.37NDD116 pKa = 3.94VFGDD120 pKa = 3.79SGDD123 pKa = 3.84SDD125 pKa = 4.62DD126 pKa = 5.94MGYY129 pKa = 10.88CSNTTVLAPVVIVAFVEE146 pKa = 4.62GTFWVVAWWLISLL159 pKa = 4.58

Molecular weight: 18.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F7W3U9|F7W3U9_SORMK MICOS complex subunit MIC10 OS=Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell) OX=771870 GN=SMAC_05330 PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.09WRR6 pKa = 11.84KK7 pKa = 8.87KK8 pKa = 8.73RR9 pKa = 11.84VRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.94RR15 pKa = 11.84KK16 pKa = 7.8RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.6MRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 11.11

Molecular weight: 3.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9890

0

9890

5012912

9

11106

506.9

55.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.667 ± 0.024

1.086 ± 0.008

5.682 ± 0.02

6.493 ± 0.03

3.418 ± 0.014

7.234 ± 0.025

2.42 ± 0.011

4.472 ± 0.016

5.153 ± 0.024

8.332 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.152 ± 0.01

3.78 ± 0.014

6.478 ± 0.032

4.211 ± 0.023

6.037 ± 0.025

8.207 ± 0.031

6.171 ± 0.021

5.991 ± 0.021

1.369 ± 0.009

2.605 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski