Amino acid dipepetide frequency for Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.068AlaAla: 10.068 ± 0.07	0.898AlaCys: 0.898 ± 0.015	4.212AlaAsp: 4.212 ± 0.029	5.36AlaGlu: 5.36 ± 0.047	2.797AlaPhe: 2.797 ± 0.025	6.086AlaGly: 6.086 ± 0.046	1.765AlaHis: 1.765 ± 0.02	3.875AlaIle: 3.875 ± 0.031	4.395AlaLys: 4.395 ± 0.038	7.189AlaLeu: 7.189 ± 0.049	1.979AlaMet: 1.979 ± 0.02	2.994AlaAsn: 2.994 ± 0.026	5.414AlaPro: 5.414 ± 0.056	3.514AlaGln: 3.514 ± 0.038	4.854AlaArg: 4.854 ± 0.041	7.33AlaSer: 7.33 ± 0.056	5.511AlaThr: 5.511 ± 0.041	5.37AlaVal: 5.37 ± 0.036	1.057AlaTrp: 1.057 ± 0.016	1.996AlaTyr: 1.996 ± 0.024	0.001AlaXaa: 0.001 ± 0.0
Cys	0.828CysAla: 0.828 ± 0.015	0.225CysCys: 0.225 ± 0.008	0.566CysAsp: 0.566 ± 0.01	0.548CysGlu: 0.548 ± 0.01	0.456CysPhe: 0.456 ± 0.009	0.869CysGly: 0.869 ± 0.015	0.291CysHis: 0.291 ± 0.007	0.564CysIle: 0.564 ± 0.01	0.496CysLys: 0.496 ± 0.012	1.088CysLeu: 1.088 ± 0.017	0.227CysMet: 0.227 ± 0.006	0.427CysAsn: 0.427 ± 0.01	0.639CysPro: 0.639 ± 0.014	0.398CysGln: 0.398 ± 0.008	0.637CysArg: 0.637 ± 0.014	0.806CysSer: 0.806 ± 0.016	0.608CysThr: 0.608 ± 0.014	0.691CysVal: 0.691 ± 0.014	0.186CysTrp: 0.186 ± 0.006	0.311CysTyr: 0.311 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	4.484AspAla: 4.484 ± 0.039	0.57AspCys: 0.57 ± 0.01	4.775AspAsp: 4.775 ± 0.054	4.821AspGlu: 4.821 ± 0.051	2.066AspPhe: 2.066 ± 0.02	4.222AspGly: 4.222 ± 0.031	1.302AspHis: 1.302 ± 0.017	2.807AspIle: 2.807 ± 0.027	2.629AspLys: 2.629 ± 0.024	4.766AspLeu: 4.766 ± 0.038	1.309AspMet: 1.309 ± 0.015	2.087AspAsn: 2.087 ± 0.021	3.334AspPro: 3.334 ± 0.025	1.881AspGln: 1.881 ± 0.02	3.004AspArg: 3.004 ± 0.035	3.946AspSer: 3.946 ± 0.034	2.858AspThr: 2.858 ± 0.026	3.539AspVal: 3.539 ± 0.031	0.85AspTrp: 0.85 ± 0.013	1.574AspTyr: 1.574 ± 0.019	0.0AspXaa: 0.0 ± 0.0
Glu	5.644GluAla: 5.644 ± 0.046	0.567GluCys: 0.567 ± 0.011	4.617GluAsp: 4.617 ± 0.047	6.953GluGlu: 6.953 ± 0.079	1.89GluPhe: 1.89 ± 0.021	4.261GluGly: 4.261 ± 0.034	1.381GluHis: 1.381 ± 0.018	2.814GluIle: 2.814 ± 0.026	4.209GluLys: 4.209 ± 0.044	5.122GluLeu: 5.122 ± 0.049	1.512GluMet: 1.512 ± 0.019	2.251GluAsn: 2.251 ± 0.02	2.923GluPro: 2.923 ± 0.039	2.732GluGln: 2.732 ± 0.028	4.217GluArg: 4.217 ± 0.04	4.02GluSer: 4.02 ± 0.032	3.466GluThr: 3.466 ± 0.03	3.833GluVal: 3.833 ± 0.035	0.905GluTrp: 0.905 ± 0.015	1.615GluTyr: 1.615 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	2.813PheAla: 2.813 ± 0.028	0.478PheCys: 0.478 ± 0.011	2.125PheAsp: 2.125 ± 0.024	2.024PheGlu: 2.024 ± 0.023	1.44PhePhe: 1.44 ± 0.02	2.739PheGly: 2.739 ± 0.029	0.84PheHis: 0.84 ± 0.014	1.551PheIle: 1.551 ± 0.021	1.497PheLys: 1.497 ± 0.018	3.099PheLeu: 3.099 ± 0.027	0.741PheMet: 0.741 ± 0.014	1.401PheAsn: 1.401 ± 0.016	1.878PhePro: 1.878 ± 0.017	1.235PheGln: 1.235 ± 0.018	1.817PheArg: 1.817 ± 0.019	2.729PheSer: 2.729 ± 0.028	2.042PheThr: 2.042 ± 0.019	2.175PheVal: 2.175 ± 0.025	0.571PheTrp: 0.571 ± 0.012	0.986PheTyr: 0.986 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	5.525GlyAla: 5.525 ± 0.044	0.805GlyCys: 0.805 ± 0.016	3.716GlyAsp: 3.716 ± 0.033	4.067GlyGlu: 4.067 ± 0.033	2.668GlyPhe: 2.668 ± 0.028	7.473GlyGly: 7.473 ± 0.075	1.864GlyHis: 1.864 ± 0.025	3.237GlyIle: 3.237 ± 0.029	3.727GlyLys: 3.727 ± 0.034	5.84GlyLeu: 5.84 ± 0.043	1.694GlyMet: 1.694 ± 0.023	2.871GlyAsn: 2.871 ± 0.034	3.706GlyPro: 3.706 ± 0.037	2.869GlyGln: 2.869 ± 0.034	4.268GlyArg: 4.268 ± 0.035	6.131GlySer: 6.131 ± 0.045	4.25GlyThr: 4.25 ± 0.04	4.363GlyVal: 4.363 ± 0.03	1.146GlyTrp: 1.146 ± 0.017	2.123GlyTyr: 2.123 ± 0.025	0.002GlyXaa: 0.002 ± 0.001
His	1.773HisAla: 1.773 ± 0.02	0.288HisCys: 0.288 ± 0.007	1.348HisAsp: 1.348 ± 0.017	1.318HisGlu: 1.318 ± 0.018	0.848HisPhe: 0.848 ± 0.014	1.825HisGly: 1.825 ± 0.02	1.165HisHis: 1.165 ± 0.021	1.091HisIle: 1.091 ± 0.015	0.968HisLys: 0.968 ± 0.014	2.127HisLeu: 2.127 ± 0.022	0.504HisMet: 0.504 ± 0.009	0.942HisAsn: 0.942 ± 0.013	1.76HisPro: 1.76 ± 0.022	1.25HisGln: 1.25 ± 0.022	1.509HisArg: 1.509 ± 0.019	1.833HisSer: 1.833 ± 0.026	1.246HisThr: 1.246 ± 0.018	1.376HisVal: 1.376 ± 0.017	0.316HisTrp: 0.316 ± 0.008	0.712HisTyr: 0.712 ± 0.012	0.001HisXaa: 0.001 ± 0.0
Ile	3.761IleAla: 3.761 ± 0.031	0.614IleCys: 0.614 ± 0.013	2.644IleAsp: 2.644 ± 0.025	2.648IleGlu: 2.648 ± 0.026	1.718IlePhe: 1.718 ± 0.023	2.822IleGly: 2.822 ± 0.029	1.093IleHis: 1.093 ± 0.016	2.27IleIle: 2.27 ± 0.025	2.256IleLys: 2.256 ± 0.019	3.948IleLeu: 3.948 ± 0.037	0.941IleMet: 0.941 ± 0.016	1.747IleAsn: 1.747 ± 0.019	3.055IlePro: 3.055 ± 0.029	1.716IleGln: 1.716 ± 0.018	2.596IleArg: 2.596 ± 0.025	3.395IleSer: 3.395 ± 0.029	2.834IleThr: 2.834 ± 0.026	2.788IleVal: 2.788 ± 0.028	0.654IleTrp: 0.654 ± 0.012	1.221IleTyr: 1.221 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	4.644LysAla: 4.644 ± 0.041	0.477LysCys: 0.477 ± 0.011	2.942LysAsp: 2.942 ± 0.028	4.105LysGlu: 4.105 ± 0.043	1.469LysPhe: 1.469 ± 0.019	3.352LysGly: 3.352 ± 0.029	1.116LysHis: 1.116 ± 0.015	2.211LysIle: 2.211 ± 0.024	4.05LysLys: 4.05 ± 0.053	4.092LysLeu: 4.092 ± 0.034	1.079LysMet: 1.079 ± 0.016	1.852LysAsn: 1.852 ± 0.021	3.032LysPro: 3.032 ± 0.032	2.015LysGln: 2.015 ± 0.024	3.593LysArg: 3.593 ± 0.032	3.425LysSer: 3.425 ± 0.033	3.049LysThr: 3.049 ± 0.031	2.98LysVal: 2.98 ± 0.025	0.72LysTrp: 0.72 ± 0.013	1.324LysTyr: 1.324 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	7.36LeuAla: 7.36 ± 0.047	1.038LeuCys: 1.038 ± 0.016	4.852LeuAsp: 4.852 ± 0.034	5.293LeuGlu: 5.293 ± 0.043	2.996LeuPhe: 2.996 ± 0.033	5.647LeuGly: 5.647 ± 0.047	1.996LeuHis: 1.996 ± 0.02	3.476LeuIle: 3.476 ± 0.031	4.165LeuLys: 4.165 ± 0.037	7.641LeuLeu: 7.641 ± 0.06	1.674LeuMet: 1.674 ± 0.019	3.034LeuAsn: 3.034 ± 0.027	5.584LeuPro: 5.584 ± 0.055	3.494LeuGln: 3.494 ± 0.033	5.382LeuArg: 5.382 ± 0.039	6.72LeuSer: 6.72 ± 0.048	4.702LeuThr: 4.702 ± 0.037	5.052LeuVal: 5.052 ± 0.034	1.109LeuTrp: 1.109 ± 0.018	2.102LeuTyr: 2.102 ± 0.021	0.001LeuXaa: 0.001 ± 0.0
Met	2.232MetAla: 2.232 ± 0.024	0.221MetCys: 0.221 ± 0.007	1.297MetAsp: 1.297 ± 0.014	1.366MetGlu: 1.366 ± 0.015	0.694MetPhe: 0.694 ± 0.013	1.645MetGly: 1.645 ± 0.021	0.445MetHis: 0.445 ± 0.011	0.891MetIle: 0.891 ± 0.015	1.012MetLys: 1.012 ± 0.014	1.704MetLeu: 1.704 ± 0.02	0.616MetMet: 0.616 ± 0.013	0.766MetAsn: 0.766 ± 0.013	1.3MetPro: 1.3 ± 0.018	0.797MetGln: 0.797 ± 0.013	1.248MetArg: 1.248 ± 0.017	1.883MetSer: 1.883 ± 0.023	1.319MetThr: 1.319 ± 0.015	1.348MetVal: 1.348 ± 0.016	0.253MetTrp: 0.253 ± 0.007	0.479MetTyr: 0.479 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	3.007AsnAla: 3.007 ± 0.026	0.406AsnCys: 0.406 ± 0.009	2.034AsnAsp: 2.034 ± 0.023	1.999AsnGlu: 1.999 ± 0.024	1.3AsnPhe: 1.3 ± 0.017	3.321AsnGly: 3.321 ± 0.04	0.951AsnHis: 0.951 ± 0.014	1.891AsnIle: 1.891 ± 0.02	1.869AsnLys: 1.869 ± 0.023	3.098AsnLeu: 3.098 ± 0.028	0.883AsnMet: 0.883 ± 0.016	2.105AsnAsn: 2.105 ± 0.035	2.626AsnPro: 2.626 ± 0.025	1.43AsnGln: 1.43 ± 0.022	1.994AsnArg: 1.994 ± 0.021	2.826AsnSer: 2.826 ± 0.028	2.334AsnThr: 2.334 ± 0.025	2.152AsnVal: 2.152 ± 0.023	0.541AsnTrp: 0.541 ± 0.01	1.037AsnTyr: 1.037 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	5.844ProAla: 5.844 ± 0.062	0.482ProCys: 0.482 ± 0.01	3.173ProAsp: 3.173 ± 0.029	3.953ProGlu: 3.953 ± 0.046	2.003ProPhe: 2.003 ± 0.022	4.312ProGly: 4.312 ± 0.037	1.397ProHis: 1.397 ± 0.018	2.586ProIle: 2.586 ± 0.021	3.031ProLys: 3.031 ± 0.034	4.78ProLeu: 4.78 ± 0.038	1.144ProMet: 1.144 ± 0.016	2.337ProAsn: 2.337 ± 0.023	6.119ProPro: 6.119 ± 0.087	2.771ProGln: 2.771 ± 0.042	3.567ProArg: 3.567 ± 0.037	6.566ProSer: 6.566 ± 0.055	4.562ProThr: 4.562 ± 0.043	3.944ProVal: 3.944 ± 0.037	0.706ProTrp: 0.706 ± 0.011	1.5ProTyr: 1.5 ± 0.017	0.001ProXaa: 0.001 ± 0.0
Gln	3.596GlnAla: 3.596 ± 0.039	0.398GlnCys: 0.398 ± 0.01	2.031GlnAsp: 2.031 ± 0.021	2.622GlnGlu: 2.622 ± 0.029	1.219GlnPhe: 1.219 ± 0.016	2.552GlnGly: 2.552 ± 0.029	1.302GlnHis: 1.302 ± 0.024	1.722GlnIle: 1.722 ± 0.018	2.092GlnLys: 2.092 ± 0.024	3.318GlnLeu: 3.318 ± 0.032	0.882GlnMet: 0.882 ± 0.017	1.523GlnAsn: 1.523 ± 0.022	3.01GlnPro: 3.01 ± 0.044	4.002GlnGln: 4.002 ± 0.098	2.663GlnArg: 2.663 ± 0.022	3.002GlnSer: 3.002 ± 0.029	2.353GlnThr: 2.353 ± 0.023	2.173GlnVal: 2.173 ± 0.022	0.508GlnTrp: 0.508 ± 0.009	1.137GlnTyr: 1.137 ± 0.016	0.001GlnXaa: 0.001 ± 0.0
Arg	4.6ArgAla: 4.6 ± 0.031	0.636ArgCys: 0.636 ± 0.012	3.363ArgAsp: 3.363 ± 0.037	4.096ArgGlu: 4.096 ± 0.036	2.016ArgPhe: 2.016 ± 0.02	3.969ArgGly: 3.969 ± 0.036	1.583ArgHis: 1.583 ± 0.018	2.64ArgIle: 2.64 ± 0.025	3.572ArgLys: 3.572 ± 0.03	5.112ArgLeu: 5.112 ± 0.033	1.322ArgMet: 1.322 ± 0.017	2.251ArgAsn: 2.251 ± 0.022	3.667ArgPro: 3.667 ± 0.038	2.679ArgGln: 2.679 ± 0.029	5.181ArgArg: 5.181 ± 0.045	4.705ArgSer: 4.705 ± 0.047	3.276ArgThr: 3.276 ± 0.024	3.259ArgVal: 3.259 ± 0.027	0.875ArgTrp: 0.875 ± 0.013	1.567ArgTyr: 1.567 ± 0.02	0.001ArgXaa: 0.001 ± 0.0
Ser	6.733SerAla: 6.733 ± 0.052	0.769SerCys: 0.769 ± 0.013	4.098SerAsp: 4.098 ± 0.042	4.034SerGlu: 4.034 ± 0.033	2.757SerPhe: 2.757 ± 0.024	5.896SerGly: 5.896 ± 0.041	1.996SerHis: 1.996 ± 0.027	3.506SerIle: 3.506 ± 0.028	3.659SerLys: 3.659 ± 0.032	6.669SerLeu: 6.669 ± 0.041	1.609SerMet: 1.609 ± 0.021	3.076SerAsn: 3.076 ± 0.029	6.005SerPro: 6.005 ± 0.076	3.283SerGln: 3.283 ± 0.031	4.871SerArg: 4.871 ± 0.046	9.843SerSer: 9.843 ± 0.097	5.851SerThr: 5.851 ± 0.047	4.444SerVal: 4.444 ± 0.033	1.01SerTrp: 1.01 ± 0.016	1.957SerTyr: 1.957 ± 0.023	0.001SerXaa: 0.001 ± 0.0
Thr	5.535ThrAla: 5.535 ± 0.038	0.692ThrCys: 0.692 ± 0.012	2.866ThrAsp: 2.866 ± 0.023	3.274ThrGlu: 3.274 ± 0.031	2.084ThrPhe: 2.084 ± 0.021	4.42ThrGly: 4.42 ± 0.038	1.298ThrHis: 1.298 ± 0.018	2.964ThrIle: 2.964 ± 0.027	2.868ThrLys: 2.868 ± 0.024	4.94ThrLeu: 4.94 ± 0.037	1.157ThrMet: 1.157 ± 0.014	2.224ThrAsn: 2.224 ± 0.023	4.843ThrPro: 4.843 ± 0.045	2.167ThrGln: 2.167 ± 0.026	3.142ThrArg: 3.142 ± 0.03	5.671ThrSer: 5.671 ± 0.044	5.551ThrThr: 5.551 ± 0.064	3.752ThrVal: 3.752 ± 0.034	0.777ThrTrp: 0.777 ± 0.012	1.486ThrTyr: 1.486 ± 0.02	0.0ThrXaa: 0.0 ± 0.0
Val	5.168ValAla: 5.168 ± 0.041	0.732ValCys: 0.732 ± 0.015	3.694ValAsp: 3.694 ± 0.03	4.06ValGlu: 4.06 ± 0.037	2.184ValPhe: 2.184 ± 0.025	3.956ValGly: 3.956 ± 0.035	1.321ValHis: 1.321 ± 0.017	2.758ValIle: 2.758 ± 0.025	3.102ValLys: 3.102 ± 0.025	5.129ValLeu: 5.129 ± 0.036	1.326ValMet: 1.326 ± 0.019	2.222ValAsn: 2.222 ± 0.022	3.832ValPro: 3.832 ± 0.035	2.264ValGln: 2.264 ± 0.019	3.383ValArg: 3.383 ± 0.027	4.442ValSer: 4.442 ± 0.036	3.544ValThr: 3.544 ± 0.032	4.334ValVal: 4.334 ± 0.04	0.834ValTrp: 0.834 ± 0.015	1.624ValTyr: 1.624 ± 0.018	0.001ValXaa: 0.001 ± 0.0
Trp	1.068TrpAla: 1.068 ± 0.016	0.191TrpCys: 0.191 ± 0.006	0.862TrpAsp: 0.862 ± 0.014	0.875TrpGlu: 0.875 ± 0.015	0.514TrpPhe: 0.514 ± 0.01	0.925TrpGly: 0.925 ± 0.015	0.33TrpHis: 0.33 ± 0.009	0.654TrpIle: 0.654 ± 0.012	0.818TrpLys: 0.818 ± 0.013	1.223TrpLeu: 1.223 ± 0.019	0.347TrpMet: 0.347 ± 0.008	0.576TrpAsn: 0.576 ± 0.012	0.582TrpPro: 0.582 ± 0.012	0.525TrpGln: 0.525 ± 0.01	0.9TrpArg: 0.9 ± 0.014	0.936TrpSer: 0.936 ± 0.015	0.851TrpThr: 0.851 ± 0.013	0.84TrpVal: 0.84 ± 0.014	0.272TrpTrp: 0.272 ± 0.008	0.399TrpTyr: 0.399 ± 0.008	0.001TrpXaa: 0.001 ± 0.0
Tyr	1.983TyrAla: 1.983 ± 0.022	0.373TyrCys: 0.373 ± 0.009	1.608TyrAsp: 1.608 ± 0.02	1.514TyrGlu: 1.514 ± 0.018	1.059TyrPhe: 1.059 ± 0.015	2.051TyrGly: 2.051 ± 0.028	0.763TyrHis: 0.763 ± 0.013	1.218TyrIle: 1.218 ± 0.018	1.111TyrLys: 1.111 ± 0.018	2.432TyrLeu: 2.432 ± 0.025	0.57TyrMet: 0.57 ± 0.012	1.119TyrAsn: 1.119 ± 0.015	1.468TyrPro: 1.468 ± 0.023	1.082TyrGln: 1.082 ± 0.016	1.541TyrArg: 1.541 ± 0.021	1.858TyrSer: 1.858 ± 0.022	1.504TyrThr: 1.504 ± 0.018	1.497TyrVal: 1.497 ± 0.017	0.401TyrTrp: 0.401 ± 0.009	0.901TyrTyr: 0.901 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.0	0.001XaaHis: 0.001 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.0	0.001XaaMet: 0.001 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.001	0.001XaaGln: 0.001 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.002XaaSer: 0.002 ± 0.001	0.001XaaThr: 0.001 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.399XaaXaa: 0.399 ± 0.066

Statistics based on 9890 proteins (5012912 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---