Mesotoga prima MesG1.Ag.4.2

Taxonomy: cellular organisms; Bacteria; Thermotogae; Thermotogae; Kosmotogales; Kosmotogaceae; Mesotoga; Mesotoga prima

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2499 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I2F6S8|I2F6S8_9BACT UPF0316 protein Theba_1988 OS=Mesotoga prima MesG1.Ag.4.2 OX=660470 GN=Theba_1988 PE=3 SV=1
MM1 pKa = 7.72KK2 pKa = 9.01KK3 pKa = 8.3TLLITLLLGFMLFVSGCFKK22 pKa = 10.77INNPPVISLTGSVSEE37 pKa = 4.33GQEE40 pKa = 3.89IAPMTLVTFEE50 pKa = 4.17WEE52 pKa = 3.72ITDD55 pKa = 4.43FDD57 pKa = 4.1DD58 pKa = 4.82TEE60 pKa = 4.33VTSEE64 pKa = 3.89FKK66 pKa = 10.74LYY68 pKa = 10.79RR69 pKa = 11.84NGAEE73 pKa = 3.98VLSVVDD79 pKa = 3.7TGEE82 pKa = 3.55AMYY85 pKa = 11.29ALDD88 pKa = 4.45EE89 pKa = 4.45EE90 pKa = 4.86GKK92 pKa = 10.33YY93 pKa = 9.46VAEE96 pKa = 4.38VVATDD101 pKa = 4.12SMGAQSKK108 pKa = 7.36EE109 pKa = 4.27TISFKK114 pKa = 10.23ATNLKK119 pKa = 10.47RR120 pKa = 11.84EE121 pKa = 4.4LFQSGAGLMYY131 pKa = 10.17FDD133 pKa = 4.33YY134 pKa = 11.13SDD136 pKa = 3.58YY137 pKa = 10.76FQYY140 pKa = 10.6IEE142 pKa = 5.19DD143 pKa = 3.73EE144 pKa = 4.17AFGIRR149 pKa = 11.84FYY151 pKa = 10.33KK152 pKa = 9.15TASEE156 pKa = 4.46EE157 pKa = 3.84ILPAEE162 pKa = 4.23EE163 pKa = 3.78VTVRR167 pKa = 11.84KK168 pKa = 9.71RR169 pKa = 11.84LNLLDD174 pKa = 3.98TDD176 pKa = 4.48DD177 pKa = 5.69DD178 pKa = 4.31GMYY181 pKa = 8.79DD182 pKa = 3.13TWEE185 pKa = 4.78EE186 pKa = 4.18IPNDD190 pKa = 3.9RR191 pKa = 11.84PDD193 pKa = 3.46LQTYY197 pKa = 9.79VYY199 pKa = 10.54LPGYY203 pKa = 9.92LIDD206 pKa = 5.8YY207 pKa = 7.71DD208 pKa = 4.47TLWVPYY214 pKa = 9.74GFEE217 pKa = 4.09YY218 pKa = 11.07GEE220 pKa = 4.02KK221 pKa = 10.22SIRR224 pKa = 11.84AYY226 pKa = 10.02YY227 pKa = 10.52AGVEE231 pKa = 4.45GPYY234 pKa = 10.03SGYY237 pKa = 10.54VLFYY241 pKa = 10.75KK242 pKa = 10.48FAEE245 pKa = 4.31VGFDD249 pKa = 3.72LNTVYY254 pKa = 10.85ARR256 pKa = 11.84WVEE259 pKa = 3.6AHH261 pKa = 6.19VFGNTLNDD269 pKa = 3.16YY270 pKa = 11.1GEE272 pKa = 4.41AYY274 pKa = 9.74KK275 pKa = 10.95AFALRR280 pKa = 11.84EE281 pKa = 4.06RR282 pKa = 11.84YY283 pKa = 10.11DD284 pKa = 3.48SDD286 pKa = 3.43EE287 pKa = 4.46KK288 pKa = 11.13PIISLSTPDD297 pKa = 4.02GDD299 pKa = 4.36IGPGDD304 pKa = 4.02SFTVSVLTEE313 pKa = 4.13NVASFADD320 pKa = 4.49LYY322 pKa = 10.82DD323 pKa = 3.28VRR325 pKa = 11.84YY326 pKa = 9.73VQLSLWNSEE335 pKa = 3.84GLVLEE340 pKa = 4.53GVEE343 pKa = 4.98FGNFMAGLKK352 pKa = 10.47DD353 pKa = 3.3VGTFKK358 pKa = 11.1SSDD361 pKa = 3.55EE362 pKa = 3.97AVTLYY367 pKa = 10.8KK368 pKa = 10.83AFLNGVDD375 pKa = 3.66EE376 pKa = 5.79AEE378 pKa = 4.33AADD381 pKa = 4.98DD382 pKa = 4.03VFATLTFSVPEE393 pKa = 4.22DD394 pKa = 3.81FEE396 pKa = 5.93GEE398 pKa = 4.24LIAVGLAYY406 pKa = 9.94EE407 pKa = 5.37GYY409 pKa = 7.99WDD411 pKa = 3.81TYY413 pKa = 8.54GTYY416 pKa = 10.12PDD418 pKa = 4.52LPNPAFRR425 pKa = 11.84DD426 pKa = 3.41VDD428 pKa = 3.66NGSVDD433 pKa = 3.57GFIVDD438 pKa = 3.95HH439 pKa = 6.41EE440 pKa = 4.76PIVFLEE446 pKa = 4.08

Molecular weight: 50.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I2F3P3|I2F3P3_9BACT Na+-transporting NADH:ubiquinone oxidoreductase subunit NqrE OS=Mesotoga prima MesG1.Ag.4.2 OX=660470 GN=Theba_0832 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.16QPSRR9 pKa = 11.84VKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84THH16 pKa = 6.49GFLVRR21 pKa = 11.84SRR23 pKa = 11.84TVGGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.61GRR39 pKa = 11.84KK40 pKa = 8.77RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2499

0

2499

811598

29

3898

324.8

36.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.618 ± 0.041

0.792 ± 0.019

5.404 ± 0.033

7.681 ± 0.06

5.019 ± 0.039

7.404 ± 0.04

1.469 ± 0.018

7.618 ± 0.042

5.951 ± 0.05

9.999 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.637 ± 0.023

3.783 ± 0.025

3.757 ± 0.028

2.218 ± 0.018

5.379 ± 0.045

7.444 ± 0.046

4.849 ± 0.032

7.516 ± 0.039

1.051 ± 0.019

3.412 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski