Amino acid dipepetide frequency for Spiribacter salinus M19-40

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.109AlaAla: 15.109 ± 0.22
1.038AlaCys: 1.038 ± 0.042
7.475AlaAsp: 7.475 ± 0.129
8.804AlaGlu: 8.804 ± 0.146
3.879AlaPhe: 3.879 ± 0.096
11.629AlaGly: 11.629 ± 0.167
2.544AlaHis: 2.544 ± 0.067
5.839AlaIle: 5.839 ± 0.097
2.038AlaLys: 2.038 ± 0.074
15.013AlaLeu: 15.013 ± 0.201
3.211AlaMet: 3.211 ± 0.079
2.482AlaAsn: 2.482 ± 0.076
5.127AlaPro: 5.127 ± 0.129
4.198AlaGln: 4.198 ± 0.092
9.372AlaArg: 9.372 ± 0.172
5.755AlaSer: 5.755 ± 0.116
5.651AlaThr: 5.651 ± 0.109
8.943AlaVal: 8.943 ± 0.146
1.738AlaTrp: 1.738 ± 0.069
2.482AlaTyr: 2.482 ± 0.072
0.0AlaXaa: 0.0 ± 0.0
Cys
0.834CysAla: 0.834 ± 0.04
0.098CysCys: 0.098 ± 0.014
0.468CysAsp: 0.468 ± 0.027
0.454CysGlu: 0.454 ± 0.027
0.28CysPhe: 0.28 ± 0.023
0.858CysGly: 0.858 ± 0.039
0.275CysHis: 0.275 ± 0.023
0.41CysIle: 0.41 ± 0.029
0.111CysLys: 0.111 ± 0.015
0.931CysLeu: 0.931 ± 0.039
0.153CysMet: 0.153 ± 0.016
0.186CysAsn: 0.186 ± 0.019
0.53CysPro: 0.53 ± 0.038
0.35CysGln: 0.35 ± 0.025
0.663CysArg: 0.663 ± 0.034
0.421CysSer: 0.421 ± 0.029
0.421CysThr: 0.421 ± 0.031
0.579CysVal: 0.579 ± 0.035
0.102CysTrp: 0.102 ± 0.013
0.215CysTyr: 0.215 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
7.526AspAla: 7.526 ± 0.139
0.543AspCys: 0.543 ± 0.033
3.652AspAsp: 3.652 ± 0.122
4.324AspGlu: 4.324 ± 0.109
2.115AspPhe: 2.115 ± 0.066
4.974AspGly: 4.974 ± 0.126
1.415AspHis: 1.415 ± 0.059
2.819AspIle: 2.819 ± 0.075
0.991AspLys: 0.991 ± 0.046
6.245AspLeu: 6.245 ± 0.115
1.209AspMet: 1.209 ± 0.044
1.484AspAsn: 1.484 ± 0.065
3.484AspPro: 3.484 ± 0.092
2.459AspGln: 2.459 ± 0.068
4.606AspArg: 4.606 ± 0.099
2.453AspSer: 2.453 ± 0.076
3.233AspThr: 3.233 ± 0.124
4.14AspVal: 4.14 ± 0.091
1.22AspTrp: 1.22 ± 0.055
1.803AspTyr: 1.803 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
9.733GluAla: 9.733 ± 0.146
0.404GluCys: 0.404 ± 0.03
3.244GluAsp: 3.244 ± 0.1
3.16GluGlu: 3.16 ± 0.088
1.614GluPhe: 1.614 ± 0.057
5.001GluGly: 5.001 ± 0.106
1.574GluHis: 1.574 ± 0.056
3.022GluIle: 3.022 ± 0.08
1.437GluLys: 1.437 ± 0.057
5.967GluLeu: 5.967 ± 0.132
1.475GluMet: 1.475 ± 0.05
1.402GluAsn: 1.402 ± 0.054
3.337GluPro: 3.337 ± 0.077
3.129GluGln: 3.129 ± 0.082
6.304GluArg: 6.304 ± 0.136
3.187GluSer: 3.187 ± 0.073
3.894GluThr: 3.894 ± 0.093
4.874GluVal: 4.874 ± 0.108
0.805GluTrp: 0.805 ± 0.036
1.136GluTyr: 1.136 ± 0.052
0.0GluXaa: 0.0 ± 0.0
Phe
3.595PheAla: 3.595 ± 0.09
0.341PheCys: 0.341 ± 0.026
2.193PheAsp: 2.193 ± 0.072
2.291PheGlu: 2.291 ± 0.065
1.2PhePhe: 1.2 ± 0.061
2.925PheGly: 2.925 ± 0.081
0.758PheHis: 0.758 ± 0.038
1.521PheIle: 1.521 ± 0.053
0.627PheLys: 0.627 ± 0.035
2.998PheLeu: 2.998 ± 0.101
0.765PheMet: 0.765 ± 0.04
0.978PheAsn: 0.978 ± 0.042
1.422PhePro: 1.422 ± 0.054
1.089PheGln: 1.089 ± 0.042
1.989PheArg: 1.989 ± 0.071
1.943PheSer: 1.943 ± 0.065
1.832PheThr: 1.832 ± 0.055
2.309PheVal: 2.309 ± 0.07
0.457PheTrp: 0.457 ± 0.029
0.82PheTyr: 0.82 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
8.697GlyAla: 8.697 ± 0.142
0.858GlyCys: 0.858 ± 0.037
5.049GlyAsp: 5.049 ± 0.121
6.034GlyGlu: 6.034 ± 0.129
3.293GlyPhe: 3.293 ± 0.087
7.103GlyGly: 7.103 ± 0.166
2.273GlyHis: 2.273 ± 0.071
4.48GlyIle: 4.48 ± 0.105
2.142GlyLys: 2.142 ± 0.071
10.258GlyLeu: 10.258 ± 0.173
2.311GlyMet: 2.311 ± 0.066
1.911GlyAsn: 1.911 ± 0.082
3.462GlyPro: 3.462 ± 0.077
3.593GlyGln: 3.593 ± 0.082
6.3GlyArg: 6.3 ± 0.11
4.209GlySer: 4.209 ± 0.095
4.265GlyThr: 4.265 ± 0.101
7.139GlyVal: 7.139 ± 0.117
1.308GlyTrp: 1.308 ± 0.063
2.339GlyTyr: 2.339 ± 0.056
0.0GlyXaa: 0.0 ± 0.0
His
2.597HisAla: 2.597 ± 0.076
0.326HisCys: 0.326 ± 0.028
1.317HisAsp: 1.317 ± 0.054
1.253HisGlu: 1.253 ± 0.051
0.867HisPhe: 0.867 ± 0.041
2.255HisGly: 2.255 ± 0.067
0.71HisHis: 0.71 ± 0.044
0.978HisIle: 0.978 ± 0.044
0.397HisLys: 0.397 ± 0.029
2.493HisLeu: 2.493 ± 0.071
0.483HisMet: 0.483 ± 0.03
0.525HisAsn: 0.525 ± 0.031
1.63HisPro: 1.63 ± 0.065
0.974HisGln: 0.974 ± 0.044
1.88HisArg: 1.88 ± 0.058
0.929HisSer: 0.929 ± 0.046
1.038HisThr: 1.038 ± 0.041
1.493HisVal: 1.493 ± 0.056
0.472HisTrp: 0.472 ± 0.03
0.716HisTyr: 0.716 ± 0.034
0.0HisXaa: 0.0 ± 0.0
Ile
6.313IleAla: 6.313 ± 0.118
0.388IleCys: 0.388 ± 0.03
3.373IleAsp: 3.373 ± 0.094
3.763IleGlu: 3.763 ± 0.089
1.286IlePhe: 1.286 ± 0.047
4.591IleGly: 4.591 ± 0.097
1.131IleHis: 1.131 ± 0.046
2.022IleIle: 2.022 ± 0.078
1.025IleLys: 1.025 ± 0.043
3.958IleLeu: 3.958 ± 0.11
0.856IleMet: 0.856 ± 0.041
1.484IleAsn: 1.484 ± 0.057
2.384IlePro: 2.384 ± 0.064
1.606IleGln: 1.606 ± 0.057
3.36IleArg: 3.36 ± 0.086
2.422IleSer: 2.422 ± 0.068
2.717IleThr: 2.717 ± 0.085
3.136IleVal: 3.136 ± 0.094
0.488IleTrp: 0.488 ± 0.031
1.018IleTyr: 1.018 ± 0.05
0.0IleXaa: 0.0 ± 0.0
Lys
2.566LysAla: 2.566 ± 0.084
0.102LysCys: 0.102 ± 0.015
1.113LysAsp: 1.113 ± 0.047
0.922LysGlu: 0.922 ± 0.055
0.435LysPhe: 0.435 ± 0.03
1.73LysGly: 1.73 ± 0.068
0.397LysHis: 0.397 ± 0.029
0.823LysIle: 0.823 ± 0.045
0.592LysLys: 0.592 ± 0.041
1.819LysLeu: 1.819 ± 0.064
0.424LysMet: 0.424 ± 0.033
0.457LysAsn: 0.457 ± 0.029
1.218LysPro: 1.218 ± 0.054
0.765LysGln: 0.765 ± 0.041
1.809LysArg: 1.809 ± 0.071
1.106LysSer: 1.106 ± 0.046
1.306LysThr: 1.306 ± 0.05
1.475LysVal: 1.475 ± 0.065
0.162LysTrp: 0.162 ± 0.019
0.397LysTyr: 0.397 ± 0.028
0.0LysXaa: 0.0 ± 0.0
Leu
14.421LeuAla: 14.421 ± 0.226
0.84LeuCys: 0.84 ± 0.04
6.846LeuAsp: 6.846 ± 0.115
7.209LeuGlu: 7.209 ± 0.142
3.286LeuPhe: 3.286 ± 0.1
9.402LeuGly: 9.402 ± 0.165
2.326LeuHis: 2.326 ± 0.077
5.08LeuIle: 5.08 ± 0.112
2.371LeuLys: 2.371 ± 0.073
10.666LeuLeu: 10.666 ± 0.205
2.57LeuMet: 2.57 ± 0.06
2.614LeuAsn: 2.614 ± 0.066
5.965LeuPro: 5.965 ± 0.135
3.823LeuGln: 3.823 ± 0.085
8.305LeuArg: 8.305 ± 0.147
6.01LeuSer: 6.01 ± 0.101
6.038LeuThr: 6.038 ± 0.122
7.824LeuVal: 7.824 ± 0.136
1.115LeuTrp: 1.115 ± 0.05
2.116LeuTyr: 2.116 ± 0.073
0.0LeuXaa: 0.0 ± 0.0
Met
3.307MetAla: 3.307 ± 0.076
0.093MetCys: 0.093 ± 0.013
1.355MetAsp: 1.355 ± 0.051
1.242MetGlu: 1.242 ± 0.05
0.506MetPhe: 0.506 ± 0.031
1.954MetGly: 1.954 ± 0.069
0.406MetHis: 0.406 ± 0.025
1.091MetIle: 1.091 ± 0.05
0.698MetLys: 0.698 ± 0.036
2.375MetLeu: 2.375 ± 0.061
0.535MetMet: 0.535 ± 0.032
0.73MetAsn: 0.73 ± 0.038
1.346MetPro: 1.346 ± 0.052
0.94MetGln: 0.94 ± 0.043
1.708MetArg: 1.708 ± 0.055
1.444MetSer: 1.444 ± 0.051
1.648MetThr: 1.648 ± 0.054
1.612MetVal: 1.612 ± 0.053
0.149MetTrp: 0.149 ± 0.013
0.264MetTyr: 0.264 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
3.018AsnAla: 3.018 ± 0.082
0.219AsnCys: 0.219 ± 0.018
1.324AsnAsp: 1.324 ± 0.06
1.337AsnGlu: 1.337 ± 0.047
0.681AsnPhe: 0.681 ± 0.039
2.091AsnGly: 2.091 ± 0.069
0.525AsnHis: 0.525 ± 0.029
1.178AsnIle: 1.178 ± 0.055
0.432AsnLys: 0.432 ± 0.033
2.519AsnLeu: 2.519 ± 0.076
0.468AsnMet: 0.468 ± 0.029
0.641AsnAsn: 0.641 ± 0.04
1.701AsnPro: 1.701 ± 0.06
0.907AsnGln: 0.907 ± 0.047
1.874AsnArg: 1.874 ± 0.055
0.914AsnSer: 0.914 ± 0.047
1.32AsnThr: 1.32 ± 0.059
1.719AsnVal: 1.719 ± 0.071
0.333AsnTrp: 0.333 ± 0.027
0.53AsnTyr: 0.53 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
6.542ProAla: 6.542 ± 0.145
0.342ProCys: 0.342 ± 0.025
3.715ProAsp: 3.715 ± 0.08
3.932ProGlu: 3.932 ± 0.084
1.513ProPhe: 1.513 ± 0.053
4.859ProGly: 4.859 ± 0.098
1.022ProHis: 1.022 ± 0.044
2.209ProIle: 2.209 ± 0.065
0.934ProLys: 0.934 ± 0.049
5.202ProLeu: 5.202 ± 0.118
1.253ProMet: 1.253 ± 0.054
1.126ProAsn: 1.126 ± 0.046
2.322ProPro: 2.322 ± 0.082
1.626ProGln: 1.626 ± 0.05
3.176ProArg: 3.176 ± 0.09
2.461ProSer: 2.461 ± 0.07
2.441ProThr: 2.441 ± 0.067
4.519ProVal: 4.519 ± 0.082
0.778ProTrp: 0.778 ± 0.04
1.044ProTyr: 1.044 ± 0.048
0.0ProXaa: 0.0 ± 0.0
Gln
5.562GlnAla: 5.562 ± 0.109
0.284GlnCys: 0.284 ± 0.026
1.725GlnAsp: 1.725 ± 0.053
1.541GlnGlu: 1.541 ± 0.058
1.187GlnPhe: 1.187 ± 0.043
2.934GlnGly: 2.934 ± 0.072
0.898GlnHis: 0.898 ± 0.043
1.772GlnIle: 1.772 ± 0.064
0.716GlnLys: 0.716 ± 0.036
3.952GlnLeu: 3.952 ± 0.088
0.829GlnMet: 0.829 ± 0.041
0.694GlnAsn: 0.694 ± 0.037
2.167GlnPro: 2.167 ± 0.062
1.63GlnGln: 1.63 ± 0.052
3.71GlnArg: 3.71 ± 0.084
1.996GlnSer: 1.996 ± 0.057
2.045GlnThr: 2.045 ± 0.065
2.952GlnVal: 2.952 ± 0.073
0.563GlnTrp: 0.563 ± 0.036
0.729GlnTyr: 0.729 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
8.107ArgAla: 8.107 ± 0.145
0.714ArgCys: 0.714 ± 0.037
4.457ArgAsp: 4.457 ± 0.099
5.382ArgGlu: 5.382 ± 0.103
3.032ArgPhe: 3.032 ± 0.071
5.48ArgGly: 5.48 ± 0.108
2.149ArgHis: 2.149 ± 0.066
3.958ArgIle: 3.958 ± 0.088
1.428ArgLys: 1.428 ± 0.057
9.551ArgLeu: 9.551 ± 0.169
1.936ArgMet: 1.936 ± 0.055
1.732ArgAsn: 1.732 ± 0.065
3.652ArgPro: 3.652 ± 0.09
3.46ArgGln: 3.46 ± 0.094
6.582ArgArg: 6.582 ± 0.144
3.51ArgSer: 3.51 ± 0.088
3.145ArgThr: 3.145 ± 0.077
5.657ArgVal: 5.657 ± 0.109
1.208ArgTrp: 1.208 ± 0.053
2.286ArgTyr: 2.286 ± 0.063
0.0ArgXaa: 0.0 ± 0.0
Ser
6.114SerAla: 6.114 ± 0.116
0.308SerCys: 0.308 ± 0.022
3.198SerAsp: 3.198 ± 0.09
3.193SerGlu: 3.193 ± 0.086
1.483SerPhe: 1.483 ± 0.056
5.142SerGly: 5.142 ± 0.108
1.162SerHis: 1.162 ± 0.056
2.155SerIle: 2.155 ± 0.063
0.821SerLys: 0.821 ± 0.042
5.376SerLeu: 5.376 ± 0.094
1.2SerMet: 1.2 ± 0.043
1.167SerAsn: 1.167 ± 0.046
2.442SerPro: 2.442 ± 0.07
1.648SerGln: 1.648 ± 0.054
3.613SerArg: 3.613 ± 0.077
2.559SerSer: 2.559 ± 0.081
2.441SerThr: 2.441 ± 0.072
3.745SerVal: 3.745 ± 0.079
0.57SerTrp: 0.57 ± 0.033
0.967SerTyr: 0.967 ± 0.041
0.0SerXaa: 0.0 ± 0.0
Thr
6.294ThrAla: 6.294 ± 0.114
0.386ThrCys: 0.386 ± 0.027
3.247ThrAsp: 3.247 ± 0.086
3.054ThrGlu: 3.054 ± 0.076
1.495ThrPhe: 1.495 ± 0.056
5.426ThrGly: 5.426 ± 0.115
1.249ThrHis: 1.249 ± 0.044
2.124ThrIle: 2.124 ± 0.095
0.765ThrLys: 0.765 ± 0.043
6.715ThrLeu: 6.715 ± 0.141
0.963ThrMet: 0.963 ± 0.038
1.085ThrAsn: 1.085 ± 0.056
3.444ThrPro: 3.444 ± 0.087
1.697ThrGln: 1.697 ± 0.058
3.666ThrArg: 3.666 ± 0.088
2.167ThrSer: 2.167 ± 0.071
2.665ThrThr: 2.665 ± 0.088
4.214ThrVal: 4.214 ± 0.119
0.616ThrTrp: 0.616 ± 0.036
0.933ThrTyr: 0.933 ± 0.037
0.0ThrXaa: 0.0 ± 0.0
Val
8.424ValAla: 8.424 ± 0.136
0.659ValCys: 0.659 ± 0.034
5.03ValAsp: 5.03 ± 0.101
4.916ValGlu: 4.916 ± 0.1
2.604ValPhe: 2.604 ± 0.078
5.772ValGly: 5.772 ± 0.107
1.59ValHis: 1.59 ± 0.06
4.255ValIle: 4.255 ± 0.1
1.523ValLys: 1.523 ± 0.059
7.952ValLeu: 7.952 ± 0.145
2.049ValMet: 2.049 ± 0.063
2.14ValAsn: 2.14 ± 0.075
3.508ValPro: 3.508 ± 0.076
2.204ValGln: 2.204 ± 0.057
5.112ValArg: 5.112 ± 0.104
4.074ValSer: 4.074 ± 0.092
4.406ValThr: 4.406 ± 0.105
6.205ValVal: 6.205 ± 0.121
0.883ValTrp: 0.883 ± 0.048
1.61ValTyr: 1.61 ± 0.054
0.0ValXaa: 0.0 ± 0.0
Trp
1.229TrpAla: 1.229 ± 0.052
0.133TrpCys: 0.133 ± 0.015
0.55TrpAsp: 0.55 ± 0.032
0.639TrpGlu: 0.639 ± 0.035
0.499TrpPhe: 0.499 ± 0.035
0.84TrpGly: 0.84 ± 0.044
0.406TrpHis: 0.406 ± 0.027
0.69TrpIle: 0.69 ± 0.038
0.215TrpLys: 0.215 ± 0.019
2.204TrpLeu: 2.204 ± 0.076
0.352TrpMet: 0.352 ± 0.025
0.324TrpAsn: 0.324 ± 0.026
0.708TrpPro: 0.708 ± 0.035
0.805TrpGln: 0.805 ± 0.038
1.237TrpArg: 1.237 ± 0.053
0.678TrpSer: 0.678 ± 0.031
0.55TrpThr: 0.55 ± 0.035
0.994TrpVal: 0.994 ± 0.044
0.244TrpTrp: 0.244 ± 0.023
0.288TrpTyr: 0.288 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.342TyrAla: 2.342 ± 0.067
0.262TyrCys: 0.262 ± 0.024
1.364TyrAsp: 1.364 ± 0.055
1.249TyrGlu: 1.249 ± 0.052
0.796TyrPhe: 0.796 ± 0.042
2.058TyrGly: 2.058 ± 0.055
0.523TyrHis: 0.523 ± 0.034
0.898TyrIle: 0.898 ± 0.042
0.357TyrLys: 0.357 ± 0.026
2.632TyrLeu: 2.632 ± 0.077
0.361TyrMet: 0.361 ± 0.024
0.568TyrAsn: 0.568 ± 0.033
1.166TyrPro: 1.166 ± 0.046
0.982TyrGln: 0.982 ± 0.042
2.158TyrArg: 2.158 ± 0.064
1.065TyrSer: 1.065 ± 0.04
1.151TyrThr: 1.151 ± 0.049
1.384TyrVal: 1.384 ± 0.051
0.375TyrTrp: 0.375 ± 0.028
0.596TyrTyr: 0.596 ± 0.034
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1684 proteins (549060 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski