Rhizobium phage vB_RleS_L338C

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 181 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V9QJG5|V9QJG5_9CAUD Terminase large subunit OS=Rhizobium phage vB_RleS_L338C OX=1414737 GN=L338C_002 PE=4 SV=1
MM1 pKa = 6.71PQVYY5 pKa = 9.65IPEE8 pKa = 4.92IGDD11 pKa = 3.35LWKK14 pKa = 11.07LEE16 pKa = 4.32TPWDD20 pKa = 3.54FTLYY24 pKa = 10.67NEE26 pKa = 4.73YY27 pKa = 10.52RR28 pKa = 11.84NSDD31 pKa = 3.02LWEE34 pKa = 4.01AA35 pKa = 3.96

Molecular weight: 4.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V9QKQ7|V9QKQ7_9CAUD dUTP diphosphatase OS=Rhizobium phage vB_RleS_L338C OX=1414737 GN=L338C_006 PE=3 SV=1
MM1 pKa = 7.46RR2 pKa = 11.84RR3 pKa = 11.84VPPSGHH9 pKa = 5.62TAVTSATQDD18 pKa = 2.81RR19 pKa = 11.84RR20 pKa = 11.84LRR22 pKa = 11.84RR23 pKa = 11.84LLAKK27 pKa = 9.86PKK29 pKa = 9.65RR30 pKa = 11.84WLSRR34 pKa = 11.84PSPSIQLFAAQATPQVRR51 pKa = 11.84SPPTWQRR58 pKa = 11.84RR59 pKa = 11.84PPVSVRR65 pKa = 11.84PTKK68 pKa = 10.45SLPASRR74 pKa = 11.84RR75 pKa = 11.84YY76 pKa = 9.31PISRR80 pKa = 11.84RR81 pKa = 11.84RR82 pKa = 11.84PGRR85 pKa = 11.84TLPPPAITFRR95 pKa = 11.84APVRR99 pKa = 11.84RR100 pKa = 3.8

Molecular weight: 11.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

181

0

181

33014

31

1651

182.4

20.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.653 ± 0.304

1.109 ± 0.1

5.949 ± 0.161

6.579 ± 0.285

3.58 ± 0.11

7.364 ± 0.205

2.22 ± 0.177

5.295 ± 0.149

5.437 ± 0.272

7.306 ± 0.178

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.868 ± 0.139

3.674 ± 0.141

4.774 ± 0.205

3.735 ± 0.149

6.512 ± 0.213

5.801 ± 0.245

6.346 ± 0.269

7.039 ± 0.165

1.581 ± 0.073

3.177 ± 0.104
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski