Avon-Heathcote Estuary associated circular virus 20

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IMJ6|A0A0C5IMJ6_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 20 OX=1618244 PE=4 SV=1
MM1 pKa = 7.42SRR3 pKa = 11.84IRR5 pKa = 11.84NMMVTIYY12 pKa = 10.66FEE14 pKa = 6.12DD15 pKa = 3.93NGCPDD20 pKa = 3.81PPEE23 pKa = 4.35IRR25 pKa = 11.84AMLGALPNLRR35 pKa = 11.84YY36 pKa = 10.37YY37 pKa = 9.77IGQMEE42 pKa = 4.61KK43 pKa = 10.58CPSTGRR49 pKa = 11.84FHH51 pKa = 6.11FQGYY55 pKa = 9.81IEE57 pKa = 5.3FDD59 pKa = 2.9SAMYY63 pKa = 9.63VKK65 pKa = 10.47KK66 pKa = 10.73ISAQLNNCHH75 pKa = 6.17IEE77 pKa = 4.0ARR79 pKa = 11.84KK80 pKa = 8.34GTQLQAIAYY89 pKa = 6.89CKK91 pKa = 9.78KK92 pKa = 9.9HH93 pKa = 4.86EE94 pKa = 4.23TRR96 pKa = 11.84VGNWIEE102 pKa = 3.73YY103 pKa = 10.0GEE105 pKa = 4.24RR106 pKa = 11.84AKK108 pKa = 10.87QGTRR112 pKa = 11.84SDD114 pKa = 3.88LDD116 pKa = 3.94DD117 pKa = 3.8VAEE120 pKa = 4.82CIYY123 pKa = 10.42GGKK126 pKa = 9.72SIRR129 pKa = 11.84EE130 pKa = 3.94IQEE133 pKa = 3.67EE134 pKa = 4.33HH135 pKa = 6.23PRR137 pKa = 11.84AYY139 pKa = 10.25MMYY142 pKa = 10.26SKK144 pKa = 10.62GIKK147 pKa = 9.62DD148 pKa = 4.06LYY150 pKa = 8.66YY151 pKa = 9.6TVNNIEE157 pKa = 4.45PRR159 pKa = 11.84SEE161 pKa = 4.11PPVVIYY167 pKa = 10.35CYY169 pKa = 10.62GQTGTGKK176 pKa = 9.6SRR178 pKa = 11.84YY179 pKa = 8.9AKK181 pKa = 9.73RR182 pKa = 11.84YY183 pKa = 8.23SDD185 pKa = 3.43NVYY188 pKa = 10.13FKK190 pKa = 11.5NMSCKK195 pKa = 9.16WWDD198 pKa = 3.72GYY200 pKa = 11.35NGQDD204 pKa = 3.02VVVFDD209 pKa = 5.97DD210 pKa = 4.16IRR212 pKa = 11.84GDD214 pKa = 3.4TFKK217 pKa = 11.07YY218 pKa = 10.44HH219 pKa = 6.1EE220 pKa = 5.06LLRR223 pKa = 11.84ILDD226 pKa = 3.37RR227 pKa = 11.84YY228 pKa = 9.12VRR230 pKa = 11.84HH231 pKa = 6.11EE232 pKa = 4.47CDD234 pKa = 3.26SPNAPMVGIPCMLNIRR250 pKa = 11.84GAASTSIRR258 pKa = 11.84LL259 pKa = 3.55

Molecular weight: 29.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IMJ6|A0A0C5IMJ6_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 20 OX=1618244 PE=4 SV=1
MM1 pKa = 7.57AFIPEE6 pKa = 5.17IIPEE10 pKa = 3.95IPEE13 pKa = 4.22IIEE16 pKa = 4.24GGEE19 pKa = 4.26ATWSAGRR26 pKa = 11.84AAVEE30 pKa = 3.63AWRR33 pKa = 11.84AGRR36 pKa = 11.84NLQAARR42 pKa = 11.84HH43 pKa = 5.24ALEE46 pKa = 3.92AARR49 pKa = 11.84RR50 pKa = 11.84AGTGIQAAEE59 pKa = 3.78RR60 pKa = 11.84TYY62 pKa = 10.85RR63 pKa = 11.84AAQRR67 pKa = 11.84HH68 pKa = 5.16YY69 pKa = 11.01NATHH73 pKa = 6.57HH74 pKa = 6.51NFGPTPPPNSPSGAPPISPASGPRR98 pKa = 11.84PTRR101 pKa = 11.84LRR103 pKa = 11.84IPRR106 pKa = 11.84TARR109 pKa = 11.84KK110 pKa = 10.13KK111 pKa = 9.71SMQVVTYY118 pKa = 9.35KK119 pKa = 10.63YY120 pKa = 9.95SRR122 pKa = 11.84GKK124 pKa = 8.96MRR126 pKa = 11.84YY127 pKa = 8.24KK128 pKa = 10.19KK129 pKa = 10.27SYY131 pKa = 8.33RR132 pKa = 11.84KK133 pKa = 9.82KK134 pKa = 10.13RR135 pKa = 11.84GRR137 pKa = 11.84KK138 pKa = 8.35YY139 pKa = 10.35KK140 pKa = 10.11RR141 pKa = 11.84KK142 pKa = 8.18TSATVPRR149 pKa = 11.84RR150 pKa = 11.84RR151 pKa = 11.84NRR153 pKa = 11.84LRR155 pKa = 11.84GYY157 pKa = 10.45HH158 pKa = 6.07KK159 pKa = 10.94GKK161 pKa = 9.6GQMMGPTRR169 pKa = 11.84AAPRR173 pKa = 11.84YY174 pKa = 8.86QLVSNGWDD182 pKa = 3.2FDD184 pKa = 4.1YY185 pKa = 10.46PYY187 pKa = 9.74MNSGDD192 pKa = 3.98KK193 pKa = 9.66VTLEE197 pKa = 3.83LTQNNGALQLVKK209 pKa = 10.68YY210 pKa = 9.93IYY212 pKa = 10.97GKK214 pKa = 10.37FAFGWGSMGMEE225 pKa = 4.24VGSEE229 pKa = 4.01IGTHH233 pKa = 5.94AYY235 pKa = 9.04LWKK238 pKa = 10.42GVKK241 pKa = 9.73FVINYY246 pKa = 7.48NIPVGLGDD254 pKa = 3.78FKK256 pKa = 11.32IEE258 pKa = 4.04FFLCARR264 pKa = 11.84AVDD267 pKa = 4.0VDD269 pKa = 4.73SNPSLVLGEE278 pKa = 4.28SPGVKK283 pKa = 9.34VVPNRR288 pKa = 11.84VMLKK292 pKa = 8.22KK293 pKa = 10.28QSYY296 pKa = 9.1KK297 pKa = 10.62VFMHH301 pKa = 6.72RR302 pKa = 11.84KK303 pKa = 7.48YY304 pKa = 11.47NMRR307 pKa = 11.84FTASRR312 pKa = 11.84DD313 pKa = 3.74TIIGSDD319 pKa = 3.82PEE321 pKa = 4.32VTKK324 pKa = 10.7AAYY327 pKa = 9.34IKK329 pKa = 10.62GGNFSPHH336 pKa = 6.86CMFKK340 pKa = 11.0NPVTVRR346 pKa = 11.84FVKK349 pKa = 9.26HH350 pKa = 5.37TGIEE354 pKa = 4.05YY355 pKa = 11.01LMLGNNSTRR364 pKa = 11.84RR365 pKa = 11.84FPIDD369 pKa = 3.1SLMMVVRR376 pKa = 11.84ILPVLRR382 pKa = 11.84GSTTSGSEE390 pKa = 4.0AVFKK394 pKa = 10.52LASFAYY400 pKa = 7.88VTKK403 pKa = 9.72EE404 pKa = 3.91VPVLYY409 pKa = 10.73VV410 pKa = 3.02

Molecular weight: 46.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

669

259

410

334.5

38.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.324 ± 1.108

1.644 ± 1.057

3.587 ± 1.272

4.933 ± 0.719

3.587 ± 0.511

8.371 ± 0.374

2.242 ± 0.043

5.979 ± 1.006

6.726 ± 0.54

5.082 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.886 ± 0.208

4.634 ± 0.223

5.979 ± 0.777

2.691 ± 0.453

8.52 ± 0.461

5.979 ± 0.331

4.933 ± 0.619

6.577 ± 0.899

1.196 ± 0.022

6.129 ± 0.92
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski