Lodeiro virus
Average proteome isoelectric point is 7.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D9CFP5|A0A1D9CFP5_9VIRU ORF3 OS=Lodeiro virus OX=1911102 PE=4 SV=1
MM1 pKa = 7.0 MVSLTGCFLLIRR13 pKa = 11.84 LLIILKK19 pKa = 9.99 IPVVLEE25 pKa = 3.82 SKK27 pKa = 9.92 YY28 pKa = 11.25 VKK30 pKa = 10.24 DD31 pKa = 3.41 LHH33 pKa = 6.84 PYY35 pKa = 8.63 IANFSQIHH43 pKa = 5.63 SLLVGRR49 pKa = 11.84 RR50 pKa = 11.84 AYY52 pKa = 10.94 LEE54 pKa = 4.56 DD55 pKa = 4.32 FNFLQRR61 pKa = 11.84 NIIMDD66 pKa = 4.43 VKK68 pKa = 8.12 PHH70 pKa = 6.72 RR71 pKa = 11.84 YY72 pKa = 9.35 VMQDD76 pKa = 2.8 RR77 pKa = 11.84 DD78 pKa = 3.13 EE79 pKa = 4.25 WNIDD83 pKa = 3.06 NCIKK87 pKa = 10.66 FIEE90 pKa = 4.36 THH92 pKa = 5.96 SLIPDD97 pKa = 4.08 DD98 pKa = 4.45 CQSILGDD105 pKa = 3.65 EE106 pKa = 4.64 CEE108 pKa = 4.17 GATFKK113 pKa = 11.11 SIEE116 pKa = 4.48 TKK118 pKa = 8.79 WHH120 pKa = 5.66 GLQLGICHH128 pKa = 6.51 PTPNCTFQDD137 pKa = 3.68 FDD139 pKa = 4.31 QSYY142 pKa = 8.76 QCGNAKK148 pKa = 9.61 YY149 pKa = 10.28 VLYY152 pKa = 10.68 HH153 pKa = 7.25 EE154 pKa = 4.54 YY155 pKa = 10.38 TFDD158 pKa = 2.94 IPYY161 pKa = 10.43 VVFVPVKK168 pKa = 10.38 RR169 pKa = 11.84 NNKK172 pKa = 5.79 VTWTAILEE180 pKa = 4.24 YY181 pKa = 10.96 NEE183 pKa = 4.5 EE184 pKa = 4.43 DD185 pKa = 3.21 LSKK188 pKa = 10.21 YY189 pKa = 9.81 QSPGSFSLKK198 pKa = 10.29 SHH200 pKa = 6.14 MQQSSYY206 pKa = 10.93 YY207 pKa = 9.39 IKK209 pKa = 10.63 QLFDD213 pKa = 3.58 RR214 pKa = 11.84 SLPDD218 pKa = 3.27 DD219 pKa = 3.91 VKK221 pKa = 11.49 YY222 pKa = 8.2 EE223 pKa = 4.06 TQIQQTEE230 pKa = 4.42 CVEE233 pKa = 4.16 SLPFYY238 pKa = 10.86 DD239 pKa = 5.07 ADD241 pKa = 3.69 GFYY244 pKa = 10.69 HH245 pKa = 7.24 EE246 pKa = 5.46 NIAYY250 pKa = 10.35 LNHH253 pKa = 6.5 DD254 pKa = 4.28 WKK256 pKa = 11.16 RR257 pKa = 11.84 MCFSLAPQGSYY268 pKa = 10.73 CDD270 pKa = 3.87 SLWWVDD276 pKa = 4.17 DD277 pKa = 4.1 YY278 pKa = 11.83 FDD280 pKa = 3.87 KK281 pKa = 9.15 TTLVKK286 pKa = 10.66 ALLEE290 pKa = 4.47 EE291 pKa = 4.3 IPWYY295 pKa = 9.82 IVYY298 pKa = 10.4 HH299 pKa = 6.63 DD300 pKa = 4.36 WNSLKK305 pKa = 10.47 QSIKK309 pKa = 10.86 DD310 pKa = 3.53 QVKK313 pKa = 8.83 TDD315 pKa = 3.85 CEE317 pKa = 4.18 IEE319 pKa = 3.99 NDD321 pKa = 2.72 IHH323 pKa = 7.15 LRR325 pKa = 11.84 WNVTHH330 pKa = 7.5 CYY332 pKa = 10.05 QGDD335 pKa = 4.56 FKK337 pKa = 11.42 CMYY340 pKa = 10.65 GPFPCSDD347 pKa = 3.11 SANRR351 pKa = 11.84 VSKK354 pKa = 10.87 CLDD357 pKa = 3.31 VEE359 pKa = 4.39 EE360 pKa = 5.55 HH361 pKa = 5.54 FTSPVTFHH369 pKa = 6.34 AVKK372 pKa = 10.41 VVRR375 pKa = 11.84 DD376 pKa = 3.79 VVKK379 pKa = 10.93 AILPYY384 pKa = 10.23 IKK386 pKa = 9.85 TIFTTVLGIIVDD398 pKa = 3.8 VFNSVLVQLGLVFMLLTTASILVMVIKK425 pKa = 10.74 FGLRR429 pKa = 11.84 YY430 pKa = 9.54 LDD432 pKa = 3.51 SSLILLTLTLLLSTLGIDD450 pKa = 4.36 LKK452 pKa = 11.12 NSYY455 pKa = 10.24 FISCILVLTASKK467 pKa = 10.36 YY468 pKa = 9.62 SKK470 pKa = 10.48 VLEE473 pKa = 4.38 RR474 pKa = 11.84 YY475 pKa = 9.41 VKK477 pKa = 10.58 EE478 pKa = 4.02 NN479 pKa = 3.03
Molecular weight: 55.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.18
IPC2_protein 5.347
IPC_protein 5.372
Toseland 5.626
ProMoST 5.664
Dawson 5.55
Bjellqvist 5.601
Wikipedia 5.525
Rodwell 5.512
Grimsley 5.703
Solomon 5.55
Lehninger 5.537
Nozaki 5.779
DTASelect 5.97
Thurlkill 5.881
EMBOSS 5.855
Sillero 5.868
Patrickios 2.511
IPC_peptide 5.563
IPC2_peptide 5.868
IPC2.peptide.svr19 5.828
Protein with the highest isoelectric point:
>tr|A0A1D9CFR9|A0A1D9CFR9_9VIRU ORF5 OS=Lodeiro virus OX=1911102 PE=4 SV=1
MM1 pKa = 7.38 AKK3 pKa = 10.08 KK4 pKa = 9.97 YY5 pKa = 6.32 QTRR8 pKa = 11.84 RR9 pKa = 11.84 TRR11 pKa = 11.84 LTAVGTARR19 pKa = 11.84 KK20 pKa = 9.58 AIRR23 pKa = 11.84 KK24 pKa = 6.76 TKK26 pKa = 9.0 TKK28 pKa = 10.52 SPKK31 pKa = 8.07 TQVLVVSNGKK41 pKa = 9.26 RR42 pKa = 11.84 KK43 pKa = 9.3 SRR45 pKa = 11.84 KK46 pKa = 9.08 RR47 pKa = 11.84 EE48 pKa = 3.69 NKK50 pKa = 9.72 KK51 pKa = 10.49 LVGVEE56 pKa = 4.07 GAYY59 pKa = 10.43 HH60 pKa = 6.04 SVVKK64 pKa = 10.39 VLNEE68 pKa = 4.1 PYY70 pKa = 10.02 TLILIGLALLVIVNYY85 pKa = 10.31 QNSKK89 pKa = 8.12 TDD91 pKa = 3.48 NVVTKK96 pKa = 10.73 LADD99 pKa = 3.78 TIGNQTSLGIWMKK112 pKa = 11.37 ANVAKK117 pKa = 10.93 VIGLAIMAPSVFTSPKK133 pKa = 9.75 SIRR136 pKa = 11.84 VPLAIGSLALVYY148 pKa = 10.03 LVKK151 pKa = 10.67 ALSLWNYY158 pKa = 9.02 FSIAIAIRR166 pKa = 11.84 VFFKK170 pKa = 11.29 VKK172 pKa = 10.05 DD173 pKa = 3.51 QNIRR177 pKa = 11.84 LIVFGLAFAMYY188 pKa = 10.13 YY189 pKa = 9.96 IGASS193 pKa = 3.3
Molecular weight: 21.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.259
IPC2_protein 10.233
IPC_protein 10.76
Toseland 10.994
ProMoST 10.584
Dawson 11.082
Bjellqvist 10.73
Wikipedia 11.242
Rodwell 11.564
Grimsley 11.111
Solomon 11.155
Lehninger 11.125
Nozaki 10.95
DTASelect 10.73
Thurlkill 10.979
EMBOSS 11.374
Sillero 10.994
Patrickios 11.286
IPC_peptide 11.169
IPC2_peptide 9.399
IPC2.peptide.svr19 8.078
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3157
109
2116
631.4
73.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.561 ± 0.773
1.742 ± 0.414
5.86 ± 0.824
7.507 ± 1.14
4.435 ± 0.537
4.53 ± 0.388
2.787 ± 0.319
6.525 ± 0.388
8.679 ± 0.877
8.933 ± 0.704
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.597 ± 0.325
4.023 ± 0.401
3.389 ± 0.15
3.833 ± 0.327
5.512 ± 0.673
6.937 ± 0.437
4.498 ± 0.639
7.697 ± 0.497
1.235 ± 0.152
4.72 ± 0.404
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here