Cohaesibacter celericrescens
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4355 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2N5XKE9|A0A2N5XKE9_9RHIZ Short-chain dehydrogenase OS=Cohaesibacter celericrescens OX=2067669 GN=C0081_22070 PE=3 SV=1
MM1 pKa = 7.44 NIKK4 pKa = 10.47 SIILGSAAALVAGGAAQAADD24 pKa = 4.15 LPVAEE29 pKa = 4.66 PVDD32 pKa = 3.96 YY33 pKa = 11.46 VKK35 pKa = 11.15 VCDD38 pKa = 4.56 AYY40 pKa = 9.79 GAGYY44 pKa = 9.68 FFIPGTDD51 pKa = 3.07 TCLKK55 pKa = 10.35 IGGKK59 pKa = 8.6 VLFGAEE65 pKa = 3.98 SAGFNSDD72 pKa = 3.01 VARR75 pKa = 11.84 SDD77 pKa = 3.45 NLVNFYY83 pKa = 10.8 TEE85 pKa = 3.99 TTFKK89 pKa = 9.9 FTARR93 pKa = 11.84 EE94 pKa = 3.91 EE95 pKa = 4.65 TEE97 pKa = 4.73 LGTLTAHH104 pKa = 6.61 MEE106 pKa = 4.04 FDD108 pKa = 3.96 DD109 pKa = 4.83 TGEE112 pKa = 4.7 DD113 pKa = 3.76 DD114 pKa = 4.74 LGEE117 pKa = 4.18 GTAIDD122 pKa = 3.68 KK123 pKa = 10.98 AYY125 pKa = 8.35 ITLGGFYY132 pKa = 10.48 AGLTDD137 pKa = 4.82 SLLSFNAGYY146 pKa = 10.5 GYY148 pKa = 10.71 DD149 pKa = 3.92 DD150 pKa = 4.36 YY151 pKa = 12.12 GVLDD155 pKa = 3.72 VDD157 pKa = 5.63 LNTIGYY163 pKa = 8.17 KK164 pKa = 10.37 AEE166 pKa = 4.0 MGNGVTAAIALEE178 pKa = 4.54 EE179 pKa = 4.12 YY180 pKa = 9.74 TGNEE184 pKa = 3.85 TGAGTSMPAVVAKK197 pKa = 10.27 LSIAQGWGSAEE208 pKa = 3.84 AGGSVFQVRR217 pKa = 11.84 YY218 pKa = 9.77 QDD220 pKa = 3.31 AAYY223 pKa = 8.5 DD224 pKa = 3.68 TDD226 pKa = 3.18 YY227 pKa = 11.42 GYY229 pKa = 11.61 AFGGQAKK236 pKa = 9.81 FNVVEE241 pKa = 4.1 DD242 pKa = 4.15 LKK244 pKa = 11.36 FGVAGGYY251 pKa = 6.5 TAGGSWLEE259 pKa = 4.06 EE260 pKa = 4.0 SAVTGKK266 pKa = 10.94 LNTGWAASAGLEE278 pKa = 4.15 YY279 pKa = 11.08 AFADD283 pKa = 3.32 NFNFYY288 pKa = 10.96 VDD290 pKa = 3.61 AGYY293 pKa = 10.71 KK294 pKa = 10.22 AFNDD298 pKa = 4.37 RR299 pKa = 11.84 SGTDD303 pKa = 3.14 DD304 pKa = 3.39 WEE306 pKa = 4.52 GWGVSAMAEE315 pKa = 4.01 YY316 pKa = 10.5 FPVKK320 pKa = 10.12 NLSIAATVGYY330 pKa = 9.95 QKK332 pKa = 10.35 IDD334 pKa = 3.39 FDD336 pKa = 4.49 TEE338 pKa = 3.88 TTANQDD344 pKa = 3.11 WDD346 pKa = 3.97 DD347 pKa = 3.76 VAAKK351 pKa = 10.65 LEE353 pKa = 4.03 IEE355 pKa = 4.26 RR356 pKa = 11.84 TFF358 pKa = 3.33
Molecular weight: 37.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.735
IPC2_protein 3.897
IPC_protein 3.91
Toseland 3.694
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.808
Rodwell 3.732
Grimsley 3.605
Solomon 3.884
Lehninger 3.834
Nozaki 3.999
DTASelect 4.215
Thurlkill 3.732
EMBOSS 3.808
Sillero 4.024
Patrickios 1.036
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.918
Protein with the highest isoelectric point:
>tr|A0A2N5XP47|A0A2N5XP47_9RHIZ DUF547 domain-containing protein OS=Cohaesibacter celericrescens OX=2067669 GN=C0081_14920 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 8.96 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.62 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.43 NGRR28 pKa = 11.84 LVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.93 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4355
0
4355
1385776
24
1854
318.2
34.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.42 ± 0.04
0.956 ± 0.011
5.919 ± 0.034
5.696 ± 0.036
4.024 ± 0.026
7.885 ± 0.034
2.109 ± 0.019
6.162 ± 0.033
4.492 ± 0.029
10.172 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.834 ± 0.019
3.319 ± 0.02
4.463 ± 0.025
3.633 ± 0.023
5.476 ± 0.034
6.407 ± 0.029
5.337 ± 0.025
7.05 ± 0.029
1.244 ± 0.013
2.403 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here