Sphingomonas sp. URHD0007

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2847 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U1G197|A0A2U1G197_9SPHN Non-homologous end joining protein Ku OS=Sphingomonas sp. URHD0007 OX=1298856 GN=ku PE=3 SV=1
MM1 pKa = 7.65PRR3 pKa = 11.84TTRR6 pKa = 11.84GPIDD10 pKa = 4.1LLSGYY15 pKa = 8.16TIIDD19 pKa = 3.76PVVGDD24 pKa = 4.08PAEE27 pKa = 3.73QDD29 pKa = 3.67YY30 pKa = 11.51YY31 pKa = 11.6EE32 pKa = 4.78MGNLTIWGVGLITFGPPTAAQQAWVADD59 pKa = 3.71GANYY63 pKa = 10.67SNLATFPGDD72 pKa = 2.89WVSFGFPIYY81 pKa = 10.28FPNATYY87 pKa = 10.57YY88 pKa = 8.19ATPIPIWSTAHH99 pKa = 5.76MVDD102 pKa = 3.34GVIVNEE108 pKa = 4.03QGIKK112 pKa = 10.52GADD115 pKa = 2.84AWGIAGQTGTGTYY128 pKa = 9.49FFSDD132 pKa = 3.14ALVTNGTNASEE143 pKa = 4.37TLTGTASAEE152 pKa = 4.22TLNGLGGDD160 pKa = 3.67DD161 pKa = 4.08TLIGGAGSDD170 pKa = 3.95ALHH173 pKa = 6.56GGAGNDD179 pKa = 3.79HH180 pKa = 6.67LVGGAGVDD188 pKa = 4.0RR189 pKa = 11.84LWGDD193 pKa = 3.82DD194 pKa = 3.88GNDD197 pKa = 3.48VLDD200 pKa = 5.04PGSDD204 pKa = 2.9AAFIYY209 pKa = 10.09VASDD213 pKa = 3.06VNRR216 pKa = 11.84AGVYY220 pKa = 9.96FVDD223 pKa = 4.86GGAGLDD229 pKa = 3.79TLVLDD234 pKa = 4.14YY235 pKa = 11.29SAATRR240 pKa = 11.84SQSISGDD247 pKa = 3.41QVLASPQVVNVEE259 pKa = 4.11ALQITGSAFSDD270 pKa = 3.83FLSGSSNADD279 pKa = 3.11QLYY282 pKa = 10.21GGGGFDD288 pKa = 3.9HH289 pKa = 7.3LSGGGGNDD297 pKa = 3.95LLDD300 pKa = 4.37AGAPGSSSVTTIGAGGHH317 pKa = 4.96STADD321 pKa = 3.54ALSLDD326 pKa = 3.69HH327 pKa = 6.91LFNAGATAPTVTFSITQTEE346 pKa = 4.57VNVVNWGFRR355 pKa = 11.84PVAGTVYY362 pKa = 10.69SFTVADD368 pKa = 4.45ASDD371 pKa = 3.54QAWIDD376 pKa = 3.48FSLTDD381 pKa = 3.98FGSEE385 pKa = 3.83VLYY388 pKa = 10.94GFTITDD394 pKa = 3.44SDD396 pKa = 4.14GVAVDD401 pKa = 3.49WFPYY405 pKa = 10.6DD406 pKa = 3.37PTVPIAFPHH415 pKa = 6.44AGTYY419 pKa = 10.09FLTVDD424 pKa = 3.71VTNTNPWAEE433 pKa = 3.98AGIDD437 pKa = 3.52VTLTLEE443 pKa = 4.51GADD446 pKa = 3.26VLAANVLEE454 pKa = 5.09GGTGDD459 pKa = 3.59DD460 pKa = 3.77TYY462 pKa = 11.79VVYY465 pKa = 10.69SATDD469 pKa = 3.27QVVEE473 pKa = 4.21NPGEE477 pKa = 4.2GTDD480 pKa = 3.7TVRR483 pKa = 11.84SSISFALVANVEE495 pKa = 4.07NLTLTGAAAINGTGNGLANIITGNAAANVITGGGGGDD532 pKa = 3.79TLTGGAGADD541 pKa = 3.4TFRR544 pKa = 11.84YY545 pKa = 9.18VALSDD550 pKa = 3.91STSAAADD557 pKa = 5.07LITDD561 pKa = 4.41FIGGKK566 pKa = 9.95KK567 pKa = 10.47GGDD570 pKa = 3.81KK571 pKa = 10.17IDD573 pKa = 4.56LSAIDD578 pKa = 4.68ANINTLANDD587 pKa = 3.7AFHH590 pKa = 6.65LVKK593 pKa = 10.74GFTGHH598 pKa = 7.32AGEE601 pKa = 5.79LYY603 pKa = 10.21TSYY606 pKa = 11.34DD607 pKa = 3.34KK608 pKa = 11.62AGGTTNIYY616 pKa = 10.67LDD618 pKa = 3.57VDD620 pKa = 3.82GDD622 pKa = 3.79RR623 pKa = 11.84AADD626 pKa = 3.71MVIHH630 pKa = 6.13LTGHH634 pKa = 6.95LNLASGDD641 pKa = 4.04FIFF644 pKa = 5.77

Molecular weight: 65.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U1FXB6|A0A2U1FXB6_9SPHN Pili/flagellar assembly PapD-like chaperone OS=Sphingomonas sp. URHD0007 OX=1298856 GN=H952_0249 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.06VLNARR34 pKa = 11.84RR35 pKa = 11.84GRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.06KK41 pKa = 10.57LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2847

0

2847

897370

29

3155

315.2

34.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.79 ± 0.069

0.774 ± 0.016

5.926 ± 0.054

5.692 ± 0.058

3.599 ± 0.026

8.83 ± 0.071

2.032 ± 0.022

4.971 ± 0.032

3.151 ± 0.034

9.903 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.185 ± 0.021

2.643 ± 0.04

5.414 ± 0.049

3.134 ± 0.026

7.329 ± 0.068

5.503 ± 0.039

5.154 ± 0.05

7.271 ± 0.038

1.472 ± 0.023

2.228 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski