Amino acid dipepetide frequency for Sphingomonas sp. URHD0007

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.571AlaAla: 18.571 ± 0.234
0.985AlaCys: 0.985 ± 0.036
7.146AlaAsp: 7.146 ± 0.1
7.666AlaGlu: 7.666 ± 0.119
4.238AlaPhe: 4.238 ± 0.078
11.137AlaGly: 11.137 ± 0.141
2.311AlaHis: 2.311 ± 0.053
6.725AlaIle: 6.725 ± 0.101
4.237AlaLys: 4.237 ± 0.086
13.328AlaLeu: 13.328 ± 0.152
3.43AlaMet: 3.43 ± 0.064
3.28AlaAsn: 3.28 ± 0.073
6.023AlaPro: 6.023 ± 0.097
4.13AlaGln: 4.13 ± 0.074
9.138AlaArg: 9.138 ± 0.144
6.662AlaSer: 6.662 ± 0.101
6.196AlaThr: 6.196 ± 0.102
8.641AlaVal: 8.641 ± 0.099
1.589AlaTrp: 1.589 ± 0.045
2.465AlaTyr: 2.465 ± 0.056
0.0AlaXaa: 0.0 ± 0.0
Cys
1.025CysAla: 1.025 ± 0.036
0.072CysCys: 0.072 ± 0.01
0.506CysAsp: 0.506 ± 0.024
0.396CysGlu: 0.396 ± 0.023
0.302CysPhe: 0.302 ± 0.019
0.857CysGly: 0.857 ± 0.035
0.194CysHis: 0.194 ± 0.016
0.327CysIle: 0.327 ± 0.018
0.169CysLys: 0.169 ± 0.013
0.691CysLeu: 0.691 ± 0.025
0.13CysMet: 0.13 ± 0.014
0.21CysAsn: 0.21 ± 0.017
0.42CysPro: 0.42 ± 0.022
0.187CysGln: 0.187 ± 0.017
0.524CysArg: 0.524 ± 0.025
0.496CysSer: 0.496 ± 0.026
0.409CysThr: 0.409 ± 0.021
0.555CysVal: 0.555 ± 0.025
0.103CysTrp: 0.103 ± 0.01
0.168CysTyr: 0.168 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
7.182AspAla: 7.182 ± 0.101
0.515AspCys: 0.515 ± 0.027
3.399AspAsp: 3.399 ± 0.092
3.919AspGlu: 3.919 ± 0.084
2.333AspPhe: 2.333 ± 0.056
5.401AspGly: 5.401 ± 0.131
1.248AspHis: 1.248 ± 0.033
2.526AspIle: 2.526 ± 0.054
1.746AspLys: 1.746 ± 0.047
5.613AspLeu: 5.613 ± 0.093
1.118AspMet: 1.118 ± 0.039
1.304AspAsn: 1.304 ± 0.045
3.835AspPro: 3.835 ± 0.066
2.016AspGln: 2.016 ± 0.051
4.67AspArg: 4.67 ± 0.082
2.752AspSer: 2.752 ± 0.072
2.629AspThr: 2.629 ± 0.114
4.379AspVal: 4.379 ± 0.063
1.075AspTrp: 1.075 ± 0.04
1.6AspTyr: 1.6 ± 0.046
0.0AspXaa: 0.0 ± 0.0
Glu
7.516GluAla: 7.516 ± 0.119
0.387GluCys: 0.387 ± 0.022
2.702GluAsp: 2.702 ± 0.064
3.234GluGlu: 3.234 ± 0.08
1.75GluPhe: 1.75 ± 0.044
4.229GluGly: 4.229 ± 0.086
1.253GluHis: 1.253 ± 0.045
2.942GluIle: 2.942 ± 0.072
1.875GluLys: 1.875 ± 0.053
6.01GluLeu: 6.01 ± 0.109
1.357GluMet: 1.357 ± 0.04
1.31GluAsn: 1.31 ± 0.052
3.066GluPro: 3.066 ± 0.059
2.419GluGln: 2.419 ± 0.057
5.482GluArg: 5.482 ± 0.098
2.553GluSer: 2.553 ± 0.065
3.054GluThr: 3.054 ± 0.054
3.963GluVal: 3.963 ± 0.082
0.856GluTrp: 0.856 ± 0.037
0.963GluTyr: 0.963 ± 0.036
0.0GluXaa: 0.0 ± 0.0
Phe
4.831PheAla: 4.831 ± 0.079
0.285PheCys: 0.285 ± 0.017
2.722PheAsp: 2.722 ± 0.058
2.222PheGlu: 2.222 ± 0.051
1.278PhePhe: 1.278 ± 0.041
3.623PheGly: 3.623 ± 0.074
0.733PheHis: 0.733 ± 0.031
1.522PheIle: 1.522 ± 0.042
0.953PheLys: 0.953 ± 0.036
3.087PheLeu: 3.087 ± 0.071
0.622PheMet: 0.622 ± 0.024
1.12PheAsn: 1.12 ± 0.034
1.555PhePro: 1.555 ± 0.043
0.944PheGln: 0.944 ± 0.032
2.186PheArg: 2.186 ± 0.052
1.988PheSer: 1.988 ± 0.046
2.03PheThr: 2.03 ± 0.047
2.796PheVal: 2.796 ± 0.058
0.527PheTrp: 0.527 ± 0.025
0.962PheTyr: 0.962 ± 0.033
0.0PheXaa: 0.0 ± 0.0
Gly
9.685GlyAla: 9.685 ± 0.138
0.859GlyCys: 0.859 ± 0.036
5.124GlyAsp: 5.124 ± 0.145
5.067GlyGlu: 5.067 ± 0.082
3.663GlyPhe: 3.663 ± 0.078
8.393GlyGly: 8.393 ± 0.221
1.948GlyHis: 1.948 ± 0.052
4.3GlyIle: 4.3 ± 0.069
3.333GlyLys: 3.333 ± 0.072
8.09GlyLeu: 8.09 ± 0.103
2.048GlyMet: 2.048 ± 0.054
2.603GlyAsn: 2.603 ± 0.135
3.88GlyPro: 3.88 ± 0.074
2.874GlyGln: 2.874 ± 0.059
6.376GlyArg: 6.376 ± 0.097
5.251GlySer: 5.251 ± 0.106
4.755GlyThr: 4.755 ± 0.114
6.168GlyVal: 6.168 ± 0.096
1.571GlyTrp: 1.571 ± 0.04
2.305GlyTyr: 2.305 ± 0.062
0.0GlyXaa: 0.0 ± 0.0
His
2.327HisAla: 2.327 ± 0.05
0.235HisCys: 0.235 ± 0.017
1.209HisAsp: 1.209 ± 0.034
1.068HisGlu: 1.068 ± 0.037
0.842HisPhe: 0.842 ± 0.035
2.053HisGly: 2.053 ± 0.046
0.573HisHis: 0.573 ± 0.028
0.937HisIle: 0.937 ± 0.033
0.54HisLys: 0.54 ± 0.025
1.971HisLeu: 1.971 ± 0.05
0.458HisMet: 0.458 ± 0.019
0.475HisAsn: 0.475 ± 0.024
1.327HisPro: 1.327 ± 0.042
0.564HisGln: 0.564 ± 0.025
1.479HisArg: 1.479 ± 0.045
1.023HisSer: 1.023 ± 0.032
0.719HisThr: 0.719 ± 0.026
1.528HisVal: 1.528 ± 0.043
0.39HisTrp: 0.39 ± 0.025
0.601HisTyr: 0.601 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
7.602IleAla: 7.602 ± 0.11
0.425IleCys: 0.425 ± 0.022
3.76IleAsp: 3.76 ± 0.063
3.575IleGlu: 3.575 ± 0.079
1.552IlePhe: 1.552 ± 0.043
4.991IleGly: 4.991 ± 0.08
0.941IleHis: 0.941 ± 0.031
2.054IleIle: 2.054 ± 0.05
1.142IleLys: 1.142 ± 0.04
4.123IleLeu: 4.123 ± 0.075
0.78IleMet: 0.78 ± 0.031
1.346IleAsn: 1.346 ± 0.045
2.315IlePro: 2.315 ± 0.047
1.138IleGln: 1.138 ± 0.041
3.202IleArg: 3.202 ± 0.061
2.591IleSer: 2.591 ± 0.058
2.418IleThr: 2.418 ± 0.068
4.169IleVal: 4.169 ± 0.079
0.55IleTrp: 0.55 ± 0.025
1.036IleTyr: 1.036 ± 0.034
0.0IleXaa: 0.0 ± 0.0
Lys
4.081LysAla: 4.081 ± 0.078
0.168LysCys: 0.168 ± 0.015
1.636LysAsp: 1.636 ± 0.049
1.365LysGlu: 1.365 ± 0.049
0.919LysPhe: 0.919 ± 0.033
2.521LysGly: 2.521 ± 0.061
0.594LysHis: 0.594 ± 0.023
1.475LysIle: 1.475 ± 0.045
1.151LysLys: 1.151 ± 0.049
3.431LysLeu: 3.431 ± 0.073
0.737LysMet: 0.737 ± 0.031
0.713LysAsn: 0.713 ± 0.026
2.147LysPro: 2.147 ± 0.053
1.035LysGln: 1.035 ± 0.039
2.546LysArg: 2.546 ± 0.051
1.826LysSer: 1.826 ± 0.052
1.672LysThr: 1.672 ± 0.047
2.36LysVal: 2.36 ± 0.058
0.456LysTrp: 0.456 ± 0.024
0.675LysTyr: 0.675 ± 0.029
0.0LysXaa: 0.0 ± 0.0
Leu
13.481LeuAla: 13.481 ± 0.152
0.732LeuCys: 0.732 ± 0.03
6.097LeuAsp: 6.097 ± 0.098
5.261LeuGlu: 5.261 ± 0.104
3.674LeuPhe: 3.674 ± 0.077
8.412LeuGly: 8.412 ± 0.091
1.833LeuHis: 1.833 ± 0.045
5.244LeuIle: 5.244 ± 0.082
3.556LeuLys: 3.556 ± 0.071
9.66LeuLeu: 9.66 ± 0.149
1.966LeuMet: 1.966 ± 0.051
2.545LeuAsn: 2.545 ± 0.05
5.287LeuPro: 5.287 ± 0.085
2.72LeuGln: 2.72 ± 0.065
6.608LeuArg: 6.608 ± 0.122
5.824LeuSer: 5.824 ± 0.092
5.621LeuThr: 5.621 ± 0.078
7.174LeuVal: 7.174 ± 0.105
1.289LeuTrp: 1.289 ± 0.043
2.048LeuTyr: 2.048 ± 0.058
0.0LeuXaa: 0.0 ± 0.0
Met
2.699MetAla: 2.699 ± 0.06
0.14MetCys: 0.14 ± 0.011
0.973MetAsp: 0.973 ± 0.034
0.9MetGlu: 0.9 ± 0.034
0.679MetPhe: 0.679 ± 0.029
1.626MetGly: 1.626 ± 0.047
0.44MetHis: 0.44 ± 0.023
1.25MetIle: 1.25 ± 0.038
0.88MetLys: 0.88 ± 0.031
2.342MetLeu: 2.342 ± 0.06
0.534MetMet: 0.534 ± 0.024
0.685MetAsn: 0.685 ± 0.031
1.295MetPro: 1.295 ± 0.036
0.657MetGln: 0.657 ± 0.028
1.785MetArg: 1.785 ± 0.05
1.429MetSer: 1.429 ± 0.041
1.527MetThr: 1.527 ± 0.038
1.46MetVal: 1.46 ± 0.04
0.25MetTrp: 0.25 ± 0.017
0.296MetTyr: 0.296 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.227AsnAla: 3.227 ± 0.075
0.247AsnCys: 0.247 ± 0.015
1.705AsnAsp: 1.705 ± 0.096
1.25AsnGlu: 1.25 ± 0.042
0.967AsnPhe: 0.967 ± 0.039
2.59AsnGly: 2.59 ± 0.096
0.486AsnHis: 0.486 ± 0.028
1.343AsnIle: 1.343 ± 0.04
0.659AsnLys: 0.659 ± 0.03
2.553AsnLeu: 2.553 ± 0.061
0.491AsnMet: 0.491 ± 0.025
0.678AsnAsn: 0.678 ± 0.035
1.83AsnPro: 1.83 ± 0.05
0.8AsnGln: 0.8 ± 0.031
1.881AsnArg: 1.881 ± 0.048
1.338AsnSer: 1.338 ± 0.051
1.162AsnThr: 1.162 ± 0.045
2.106AsnVal: 2.106 ± 0.063
0.448AsnTrp: 0.448 ± 0.022
0.672AsnTyr: 0.672 ± 0.029
0.0AsnXaa: 0.0 ± 0.0
Pro
6.723ProAla: 6.723 ± 0.096
0.319ProCys: 0.319 ± 0.019
3.775ProAsp: 3.775 ± 0.073
3.509ProGlu: 3.509 ± 0.072
1.911ProPhe: 1.911 ± 0.041
4.78ProGly: 4.78 ± 0.082
1.111ProHis: 1.111 ± 0.034
2.54ProIle: 2.54 ± 0.049
1.785ProLys: 1.785 ± 0.045
4.986ProLeu: 4.986 ± 0.085
1.21ProMet: 1.21 ± 0.036
1.474ProAsn: 1.474 ± 0.046
3.531ProPro: 3.531 ± 0.106
1.799ProGln: 1.799 ± 0.043
3.195ProArg: 3.195 ± 0.059
2.893ProSer: 2.893 ± 0.058
2.586ProThr: 2.586 ± 0.053
4.101ProVal: 4.101 ± 0.083
0.783ProTrp: 0.783 ± 0.028
1.13ProTyr: 1.13 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
3.979GlnAla: 3.979 ± 0.068
0.208GlnCys: 0.208 ± 0.017
1.342GlnAsp: 1.342 ± 0.042
1.335GlnGlu: 1.335 ± 0.037
1.113GlnPhe: 1.113 ± 0.038
2.266GlnGly: 2.266 ± 0.06
0.671GlnHis: 0.671 ± 0.027
1.639GlnIle: 1.639 ± 0.041
1.0GlnLys: 1.0 ± 0.034
3.566GlnLeu: 3.566 ± 0.072
0.741GlnMet: 0.741 ± 0.031
0.779GlnAsn: 0.779 ± 0.033
2.029GlnPro: 2.029 ± 0.061
1.269GlnGln: 1.269 ± 0.045
2.594GlnArg: 2.594 ± 0.059
1.832GlnSer: 1.832 ± 0.044
1.565GlnThr: 1.565 ± 0.043
2.28GlnVal: 2.28 ± 0.055
0.457GlnTrp: 0.457 ± 0.023
0.676GlnTyr: 0.676 ± 0.029
0.0GlnXaa: 0.0 ± 0.0
Arg
8.245ArgAla: 8.245 ± 0.13
0.489ArgCys: 0.489 ± 0.027
4.215ArgAsp: 4.215 ± 0.074
4.309ArgGlu: 4.309 ± 0.086
3.078ArgPhe: 3.078 ± 0.069
5.242ArgGly: 5.242 ± 0.086
1.634ArgHis: 1.634 ± 0.044
4.025ArgIle: 4.025 ± 0.076
2.277ArgLys: 2.277 ± 0.051
8.268ArgLeu: 8.268 ± 0.126
1.832ArgMet: 1.832 ± 0.048
1.854ArgAsn: 1.854 ± 0.046
3.748ArgPro: 3.748 ± 0.074
2.432ArgGln: 2.432 ± 0.061
5.879ArgArg: 5.879 ± 0.104
4.191ArgSer: 4.191 ± 0.069
3.58ArgThr: 3.58 ± 0.073
4.92ArgVal: 4.92 ± 0.082
1.241ArgTrp: 1.241 ± 0.036
1.835ArgTyr: 1.835 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
6.916SerAla: 6.916 ± 0.097
0.399SerCys: 0.399 ± 0.023
3.322SerAsp: 3.322 ± 0.069
3.0SerGlu: 3.0 ± 0.067
2.116SerPhe: 2.116 ± 0.061
5.83SerGly: 5.83 ± 0.107
1.033SerHis: 1.033 ± 0.032
2.685SerIle: 2.685 ± 0.071
1.545SerLys: 1.545 ± 0.045
5.318SerLeu: 5.318 ± 0.08
1.14SerMet: 1.14 ± 0.04
1.553SerAsn: 1.553 ± 0.045
2.917SerPro: 2.917 ± 0.061
1.527SerGln: 1.527 ± 0.046
3.751SerArg: 3.751 ± 0.073
3.213SerSer: 3.213 ± 0.067
2.701SerThr: 2.701 ± 0.058
3.803SerVal: 3.803 ± 0.073
0.918SerTrp: 0.918 ± 0.037
1.344SerTyr: 1.344 ± 0.039
0.0SerXaa: 0.0 ± 0.0
Thr
6.275ThrAla: 6.275 ± 0.095
0.408ThrCys: 0.408 ± 0.024
2.966ThrAsp: 2.966 ± 0.081
2.292ThrGlu: 2.292 ± 0.048
1.89ThrPhe: 1.89 ± 0.043
5.178ThrGly: 5.178 ± 0.098
1.006ThrHis: 1.006 ± 0.032
3.011ThrIle: 3.011 ± 0.066
1.348ThrLys: 1.348 ± 0.043
5.386ThrLeu: 5.386 ± 0.099
1.074ThrMet: 1.074 ± 0.034
1.45ThrAsn: 1.45 ± 0.056
3.076ThrPro: 3.076 ± 0.065
1.471ThrGln: 1.471 ± 0.044
3.325ThrArg: 3.325 ± 0.062
2.802ThrSer: 2.802 ± 0.066
2.629ThrThr: 2.629 ± 0.075
4.154ThrVal: 4.154 ± 0.104
0.606ThrTrp: 0.606 ± 0.029
1.187ThrTyr: 1.187 ± 0.04
0.0ThrXaa: 0.0 ± 0.0
Val
9.47ValAla: 9.47 ± 0.133
0.552ValCys: 0.552 ± 0.025
4.456ValAsp: 4.456 ± 0.072
4.72ValGlu: 4.72 ± 0.084
2.205ValPhe: 2.205 ± 0.044
5.942ValGly: 5.942 ± 0.103
1.46ValHis: 1.46 ± 0.046
3.67ValIle: 3.67 ± 0.067
2.195ValLys: 2.195 ± 0.055
6.612ValLeu: 6.612 ± 0.11
1.456ValMet: 1.456 ± 0.041
1.967ValAsn: 1.967 ± 0.053
4.056ValPro: 4.056 ± 0.071
2.146ValGln: 2.146 ± 0.053
5.429ValArg: 5.429 ± 0.089
4.188ValSer: 4.188 ± 0.068
4.44ValThr: 4.44 ± 0.128
5.48ValVal: 5.48 ± 0.087
0.919ValTrp: 0.919 ± 0.04
1.344ValTyr: 1.344 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.429TrpAla: 1.429 ± 0.044
0.113TrpCys: 0.113 ± 0.011
0.733TrpAsp: 0.733 ± 0.025
0.576TrpGlu: 0.576 ± 0.021
0.538TrpPhe: 0.538 ± 0.025
0.987TrpGly: 0.987 ± 0.036
0.376TrpHis: 0.376 ± 0.023
0.74TrpIle: 0.74 ± 0.028
0.524TrpLys: 0.524 ± 0.025
1.864TrpLeu: 1.864 ± 0.052
0.362TrpMet: 0.362 ± 0.022
0.47TrpAsn: 0.47 ± 0.023
0.778TrpPro: 0.778 ± 0.03
0.532TrpGln: 0.532 ± 0.021
1.376TrpArg: 1.376 ± 0.044
0.922TrpSer: 0.922 ± 0.031
0.854TrpThr: 0.854 ± 0.034
0.957TrpVal: 0.957 ± 0.038
0.283TrpTrp: 0.283 ± 0.019
0.304TrpTyr: 0.304 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.633TyrAla: 2.633 ± 0.065
0.202TyrCys: 0.202 ± 0.016
1.473TyrAsp: 1.473 ± 0.043
1.256TyrGlu: 1.256 ± 0.038
0.936TyrPhe: 0.936 ± 0.032
2.238TyrGly: 2.238 ± 0.069
0.474TyrHis: 0.474 ± 0.026
0.754TyrIle: 0.754 ± 0.031
0.594TyrLys: 0.594 ± 0.025
2.134TyrLeu: 2.134 ± 0.057
0.361TyrMet: 0.361 ± 0.021
0.617TyrAsn: 0.617 ± 0.033
1.027TyrPro: 1.027 ± 0.036
0.682TyrGln: 0.682 ± 0.031
1.867TyrArg: 1.867 ± 0.049
1.258TyrSer: 1.258 ± 0.039
0.988TyrThr: 0.988 ± 0.039
1.713TyrVal: 1.713 ± 0.044
0.405TyrTrp: 0.405 ± 0.021
0.664TyrTyr: 0.664 ± 0.029
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2847 proteins (897370 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski