Camelimonas lactis
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3613 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R2GJR6|A0A4R2GJR6_9RHIZ Rhomboid family protein OS=Camelimonas lactis OX=659006 GN=EV666_1194 PE=4 SV=1
MM1 pKa = 8.12 AIPDD5 pKa = 3.62 HH6 pKa = 6.79 ARR8 pKa = 11.84 TNFNTLLRR16 pKa = 11.84 AAGDD20 pKa = 3.71 GNLALMEE27 pKa = 4.67 CLDD30 pKa = 3.76 AVTCEE35 pKa = 3.67 RR36 pKa = 11.84 RR37 pKa = 11.84 YY38 pKa = 9.73 VVCAVGRR45 pKa = 11.84 ADD47 pKa = 5.32 DD48 pKa = 4.37 GDD50 pKa = 3.6 IVMTPFGHH58 pKa = 7.34 LADD61 pKa = 4.46 GNPYY65 pKa = 10.74 DD66 pKa = 5.49 LYY68 pKa = 11.19 LPPDD72 pKa = 4.56 PDD74 pKa = 4.15 DD75 pKa = 3.76 PAGFIEE81 pKa = 4.07 NPEE84 pKa = 4.45 DD85 pKa = 3.82 GGASS89 pKa = 3.32
Molecular weight: 9.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.923
IPC_protein 3.884
Toseland 3.668
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.872
Rodwell 3.719
Grimsley 3.579
Solomon 3.884
Lehninger 3.846
Nozaki 4.024
DTASelect 4.291
Thurlkill 3.745
EMBOSS 3.872
Sillero 4.024
Patrickios 1.939
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.905
Protein with the highest isoelectric point:
>tr|A0A4R2GT76|A0A4R2GT76_9RHIZ Uncharacterized protein OS=Camelimonas lactis OX=659006 GN=EV666_10537 PE=4 SV=1
MM1 pKa = 7.35 VFLLKK6 pKa = 9.86 PQKK9 pKa = 10.39 AAARR13 pKa = 11.84 KK14 pKa = 7.67 PAARR18 pKa = 11.84 SSAASRR24 pKa = 11.84 TTTRR28 pKa = 11.84 AAPKK32 pKa = 10.16 KK33 pKa = 8.66 PAARR37 pKa = 11.84 KK38 pKa = 8.06 ATATKK43 pKa = 10.48 APARR47 pKa = 11.84 KK48 pKa = 7.52 TATTKK53 pKa = 9.58 TAAAKK58 pKa = 9.29 TRR60 pKa = 11.84 ATAARR65 pKa = 11.84 APARR69 pKa = 11.84 KK70 pKa = 8.38 PAARR74 pKa = 11.84 TTTTRR79 pKa = 11.84 ATAAKK84 pKa = 9.51 KK85 pKa = 9.17 PAVRR89 pKa = 11.84 KK90 pKa = 8.93 AAARR94 pKa = 11.84 PAHH97 pKa = 6.93 RR98 pKa = 11.84 GRR100 pKa = 4.37
Molecular weight: 10.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.512
IPC2_protein 11.418
IPC_protein 12.983
Toseland 13.159
ProMoST 13.656
Dawson 13.159
Bjellqvist 13.144
Wikipedia 13.627
Rodwell 12.954
Grimsley 13.203
Solomon 13.656
Lehninger 13.554
Nozaki 13.159
DTASelect 13.144
Thurlkill 13.159
EMBOSS 13.656
Sillero 13.159
Patrickios 12.661
IPC_peptide 13.656
IPC2_peptide 12.632
IPC2.peptide.svr19 9.267
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3613
0
3613
1159828
26
2966
321.0
34.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.095 ± 0.064
0.811 ± 0.013
5.691 ± 0.032
5.103 ± 0.036
3.51 ± 0.027
9.129 ± 0.049
1.939 ± 0.016
4.665 ± 0.026
2.828 ± 0.029
9.781 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.486 ± 0.019
2.513 ± 0.033
5.572 ± 0.038
3.186 ± 0.023
7.757 ± 0.046
5.052 ± 0.033
4.991 ± 0.03
7.454 ± 0.033
1.373 ± 0.016
2.066 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here