Streptomyces spinoverrucosus

Taxonomy:

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8452 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y3VM05|A0A4Y3VM05_9ACTN Uncharacterized protein OS=Streptomyces spinoverrucosus OX=284043 GN=SSP24_55320 PE=4 SV=1
MM1 pKa = 7.1YY2 pKa = 10.28SVYY5 pKa = 10.04DD6 pKa = 3.74TAQEE10 pKa = 3.72ITDD13 pKa = 3.71YY14 pKa = 11.67LNAFVAADD22 pKa = 3.82LPILIGEE29 pKa = 4.67FGGPPDD35 pKa = 3.3QWGDD39 pKa = 3.55PDD41 pKa = 4.84EE42 pKa = 4.52DD43 pKa = 3.75TMMAAAEE50 pKa = 4.0QLDD53 pKa = 4.24LGYY56 pKa = 10.47LAWSWSGNTDD66 pKa = 4.46PILDD70 pKa = 4.08LAIDD74 pKa = 4.3FDD76 pKa = 4.53PSRR79 pKa = 11.84LSSWGQRR86 pKa = 11.84IFHH89 pKa = 6.64GANGIAQTSKK99 pKa = 10.04EE100 pKa = 3.85ATIFGGGTPTDD111 pKa = 4.03TQPPTAPGTPSASAVTSTSVTLTWPAATDD140 pKa = 3.63NVRR143 pKa = 11.84VTAYY147 pKa = 10.61DD148 pKa = 3.54VVRR151 pKa = 11.84VAGASEE157 pKa = 3.86PTAASSATDD166 pKa = 3.28TATVSGLTAGTTYY179 pKa = 10.61TFAVYY184 pKa = 10.54ARR186 pKa = 11.84DD187 pKa = 3.5ATGNRR192 pKa = 11.84SPRR195 pKa = 11.84SATVTVTTADD205 pKa = 3.69APAVACSVGYY215 pKa = 9.84RR216 pKa = 11.84VVNEE220 pKa = 4.02WPGGFQGEE228 pKa = 4.36VVIRR232 pKa = 11.84NTGTTPLNGWTLAFTFTDD250 pKa = 3.86GQTITNMWGGTASQTGGAVTVTPASYY276 pKa = 10.36TSTIPAGGSVMVGFVGASGATNTAPTAFTLNGGNCAAGG314 pKa = 3.28

Molecular weight: 32.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y3VUR0|A0A4Y3VUR0_9ACTN DDE_Tnp_ISL3 domain-containing protein OS=Streptomyces spinoverrucosus OX=284043 GN=SSP24_83590 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8452

0

8452

2674752

39

6275

316.5

33.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.282 ± 0.038

0.807 ± 0.007

6.008 ± 0.02

5.747 ± 0.028

2.713 ± 0.015

9.31 ± 0.025

2.317 ± 0.012

3.265 ± 0.015

2.217 ± 0.02

10.253 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.748 ± 0.009

1.859 ± 0.014

6.072 ± 0.022

2.899 ± 0.016

8.006 ± 0.029

5.021 ± 0.02

6.297 ± 0.023

8.445 ± 0.022

1.561 ± 0.012

2.173 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski