Methanocella paludicola (strain DSM 17711 / JCM 13418 / NBRC 101707 / SANAE)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanocellales; Methanocellaceae; Methanocella; Methanocella paludicola

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2993 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D1Z025|D1Z025_METPS Tryptophan synthase beta chain OS=Methanocella paludicola (strain DSM 17711 / JCM 13418 / NBRC 101707 / SANAE) OX=304371 GN=trpB PE=3 SV=1
MM1 pKa = 7.7PLIIDD6 pKa = 4.39DD7 pKa = 4.38EE8 pKa = 5.07GISDD12 pKa = 4.14VLAVILIAALTIIIASIMASYY33 pKa = 10.64AYY35 pKa = 10.43GLAQDD40 pKa = 3.46IRR42 pKa = 11.84QPKK45 pKa = 8.48NVVVTAQRR53 pKa = 11.84VDD55 pKa = 3.26TTTITLTNSGGDD67 pKa = 3.87LEE69 pKa = 5.35ILDD72 pKa = 3.96TTTPFRR78 pKa = 11.84VVINDD83 pKa = 3.2IEE85 pKa = 4.68VPCGNLDD92 pKa = 3.69EE93 pKa = 5.88NIGSTEE99 pKa = 3.89VFNANAGAHH108 pKa = 4.54VVVTAICYY116 pKa = 9.87QEE118 pKa = 4.28TPKK121 pKa = 10.86VIYY124 pKa = 10.4DD125 pKa = 4.49GILL128 pKa = 3.12

Molecular weight: 13.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D1YVC0|D1YVC0_METPS Uncharacterized protein OS=Methanocella paludicola (strain DSM 17711 / JCM 13418 / NBRC 101707 / SANAE) OX=304371 GN=MCP_0320 PE=4 SV=1
MM1 pKa = 7.7PEE3 pKa = 3.74RR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84KK7 pKa = 9.22EE8 pKa = 3.92PQRR11 pKa = 11.84KK12 pKa = 8.06PFNVDD17 pKa = 2.64MAEE20 pKa = 3.88QRR22 pKa = 11.84IKK24 pKa = 10.9RR25 pKa = 11.84LFEE28 pKa = 4.13LAEE31 pKa = 3.67QAYY34 pKa = 9.44PGRR37 pKa = 11.84PDD39 pKa = 3.47LADD42 pKa = 4.33RR43 pKa = 11.84YY44 pKa = 10.9ADD46 pKa = 3.12IARR49 pKa = 11.84RR50 pKa = 11.84ISMRR54 pKa = 11.84HH55 pKa = 4.02RR56 pKa = 11.84VGIPRR61 pKa = 11.84EE62 pKa = 3.96LKK64 pKa = 10.02RR65 pKa = 11.84RR66 pKa = 11.84VCKK69 pKa = 10.6GCGSFLVPGDD79 pKa = 3.58NCRR82 pKa = 11.84VRR84 pKa = 11.84LDD86 pKa = 3.64GNNIVITCMKK96 pKa = 9.71CGAIKK101 pKa = 10.27RR102 pKa = 11.84YY103 pKa = 8.9PYY105 pKa = 10.56RR106 pKa = 5.37

Molecular weight: 12.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2993

0

2993

852371

29

1701

284.8

31.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.565 ± 0.055

1.254 ± 0.023

5.778 ± 0.036

6.215 ± 0.053

3.749 ± 0.03

7.951 ± 0.05

1.727 ± 0.018

7.069 ± 0.043

6.138 ± 0.049

9.188 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.16 ± 0.024

3.581 ± 0.036

4.462 ± 0.035

2.359 ± 0.02

5.245 ± 0.046

6.078 ± 0.04

5.207 ± 0.049

7.681 ± 0.037

0.954 ± 0.014

3.641 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski