Amino acid dipepetide frequency for Methanocella paludicola (strain DSM 17711 / JCM 13418 / NBRC 101707 / SANAE)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.143AlaAla: 8.143 ± 0.15
1.224AlaCys: 1.224 ± 0.041
4.423AlaAsp: 4.423 ± 0.072
4.827AlaGlu: 4.827 ± 0.092
3.367AlaPhe: 3.367 ± 0.069
7.225AlaGly: 7.225 ± 0.103
1.346AlaHis: 1.346 ± 0.044
6.512AlaIle: 6.512 ± 0.098
4.375AlaLys: 4.375 ± 0.076
9.151AlaLeu: 9.151 ± 0.124
2.929AlaMet: 2.929 ± 0.052
2.385AlaAsn: 2.385 ± 0.059
3.08AlaPro: 3.08 ± 0.075
1.98AlaGln: 1.98 ± 0.047
4.991AlaArg: 4.991 ± 0.092
5.093AlaSer: 5.093 ± 0.082
3.958AlaThr: 3.958 ± 0.084
6.935AlaVal: 6.935 ± 0.117
0.792AlaTrp: 0.792 ± 0.033
2.914AlaTyr: 2.914 ± 0.063
0.0AlaXaa: 0.0 ± 0.0
Cys
0.944CysAla: 0.944 ± 0.034
0.228CysCys: 0.228 ± 0.018
0.786CysAsp: 0.786 ± 0.03
0.723CysGlu: 0.723 ± 0.028
0.398CysPhe: 0.398 ± 0.022
1.444CysGly: 1.444 ± 0.058
0.289CysHis: 0.289 ± 0.021
0.863CysIle: 0.863 ± 0.032
0.58CysLys: 0.58 ± 0.029
0.944CysLeu: 0.944 ± 0.035
0.372CysMet: 0.372 ± 0.023
0.443CysAsn: 0.443 ± 0.023
0.869CysPro: 0.869 ± 0.039
0.248CysGln: 0.248 ± 0.016
0.732CysArg: 0.732 ± 0.033
0.711CysSer: 0.711 ± 0.03
0.656CysThr: 0.656 ± 0.026
0.835CysVal: 0.835 ± 0.032
0.106CysTrp: 0.106 ± 0.012
0.372CysTyr: 0.372 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
4.653AspAla: 4.653 ± 0.079
0.612AspCys: 0.612 ± 0.026
2.986AspAsp: 2.986 ± 0.065
3.915AspGlu: 3.915 ± 0.081
2.224AspPhe: 2.224 ± 0.053
4.561AspGly: 4.561 ± 0.087
1.028AspHis: 1.028 ± 0.036
5.052AspIle: 5.052 ± 0.083
3.222AspLys: 3.222 ± 0.056
5.025AspLeu: 5.025 ± 0.086
2.034AspMet: 2.034 ± 0.051
2.072AspAsn: 2.072 ± 0.059
2.616AspPro: 2.616 ± 0.056
1.038AspGln: 1.038 ± 0.036
3.352AspArg: 3.352 ± 0.076
2.917AspSer: 2.917 ± 0.053
2.827AspThr: 2.827 ± 0.057
4.864AspVal: 4.864 ± 0.08
0.616AspTrp: 0.616 ± 0.029
2.161AspTyr: 2.161 ± 0.058
0.0AspXaa: 0.0 ± 0.0
Glu
5.983GluAla: 5.983 ± 0.103
0.7GluCys: 0.7 ± 0.032
3.27GluAsp: 3.27 ± 0.066
4.437GluGlu: 4.437 ± 0.097
2.192GluPhe: 2.192 ± 0.053
4.148GluGly: 4.148 ± 0.071
1.293GluHis: 1.293 ± 0.039
3.819GluIle: 3.819 ± 0.062
4.992GluLys: 4.992 ± 0.09
5.938GluLeu: 5.938 ± 0.093
1.912GluMet: 1.912 ± 0.056
2.325GluAsn: 2.325 ± 0.059
2.249GluPro: 2.249 ± 0.059
1.706GluGln: 1.706 ± 0.049
3.806GluArg: 3.806 ± 0.071
3.369GluSer: 3.369 ± 0.064
2.757GluThr: 2.757 ± 0.063
4.397GluVal: 4.397 ± 0.075
0.576GluTrp: 0.576 ± 0.025
2.282GluTyr: 2.282 ± 0.054
0.0GluXaa: 0.0 ± 0.0
Phe
2.805PheAla: 2.805 ± 0.063
0.503PheCys: 0.503 ± 0.025
2.343PheAsp: 2.343 ± 0.051
2.163PheGlu: 2.163 ± 0.051
1.513PhePhe: 1.513 ± 0.048
3.264PheGly: 3.264 ± 0.073
0.635PheHis: 0.635 ± 0.029
3.032PheIle: 3.032 ± 0.064
2.028PheLys: 2.028 ± 0.051
3.38PheLeu: 3.38 ± 0.065
1.143PheMet: 1.143 ± 0.039
1.625PheAsn: 1.625 ± 0.044
1.389PhePro: 1.389 ± 0.047
0.819PheGln: 0.819 ± 0.034
1.695PheArg: 1.695 ± 0.043
2.454PheSer: 2.454 ± 0.054
2.141PheThr: 2.141 ± 0.052
2.737PheVal: 2.737 ± 0.06
0.38PheTrp: 0.38 ± 0.022
1.436PheTyr: 1.436 ± 0.041
0.0PheXaa: 0.0 ± 0.0
Gly
6.158GlyAla: 6.158 ± 0.108
1.195GlyCys: 1.195 ± 0.045
4.249GlyAsp: 4.249 ± 0.077
4.534GlyGlu: 4.534 ± 0.082
3.172GlyPhe: 3.172 ± 0.077
5.715GlyGly: 5.715 ± 0.112
1.604GlyHis: 1.604 ± 0.047
6.282GlyIle: 6.282 ± 0.097
5.03GlyLys: 5.03 ± 0.083
7.423GlyLeu: 7.423 ± 0.11
2.721GlyMet: 2.721 ± 0.065
2.809GlyAsn: 2.809 ± 0.064
2.664GlyPro: 2.664 ± 0.062
1.984GlyGln: 1.984 ± 0.051
4.618GlyArg: 4.618 ± 0.086
4.875GlySer: 4.875 ± 0.091
4.513GlyThr: 4.513 ± 0.085
5.76GlyVal: 5.76 ± 0.101
0.942GlyTrp: 0.942 ± 0.041
3.265GlyTyr: 3.265 ± 0.067
0.0GlyXaa: 0.0 ± 0.0
His
1.347HisAla: 1.347 ± 0.044
0.264HisCys: 0.264 ± 0.019
1.146HisAsp: 1.146 ± 0.039
1.071HisGlu: 1.071 ± 0.039
0.603HisPhe: 0.603 ± 0.027
1.444HisGly: 1.444 ± 0.048
0.361HisHis: 0.361 ± 0.021
1.285HisIle: 1.285 ± 0.042
0.902HisLys: 0.902 ± 0.034
1.346HisLeu: 1.346 ± 0.041
0.612HisMet: 0.612 ± 0.027
0.596HisAsn: 0.596 ± 0.024
1.001HisPro: 1.001 ± 0.031
0.367HisGln: 0.367 ± 0.019
0.856HisArg: 0.856 ± 0.033
0.921HisSer: 0.921 ± 0.034
0.926HisThr: 0.926 ± 0.031
1.337HisVal: 1.337 ± 0.036
0.171HisTrp: 0.171 ± 0.014
0.712HisTyr: 0.712 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
6.194IleAla: 6.194 ± 0.086
0.807IleCys: 0.807 ± 0.035
4.275IleAsp: 4.275 ± 0.073
4.282IleGlu: 4.282 ± 0.09
2.676IlePhe: 2.676 ± 0.059
5.621IleGly: 5.621 ± 0.11
1.082IleHis: 1.082 ± 0.034
5.005IleIle: 5.005 ± 0.099
4.431IleLys: 4.431 ± 0.098
6.23IleLeu: 6.23 ± 0.094
2.084IleMet: 2.084 ± 0.055
2.521IleAsn: 2.521 ± 0.059
3.109IlePro: 3.109 ± 0.064
1.563IleGln: 1.563 ± 0.041
3.49IleArg: 3.49 ± 0.069
4.667IleSer: 4.667 ± 0.08
3.926IleThr: 3.926 ± 0.066
5.895IleVal: 5.895 ± 0.085
0.549IleTrp: 0.549 ± 0.03
2.278IleTyr: 2.278 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
5.674LysAla: 5.674 ± 0.086
0.795LysCys: 0.795 ± 0.038
3.956LysAsp: 3.956 ± 0.08
4.539LysGlu: 4.539 ± 0.094
1.837LysPhe: 1.837 ± 0.045
4.599LysGly: 4.599 ± 0.079
0.995LysHis: 0.995 ± 0.035
3.644LysIle: 3.644 ± 0.07
5.081LysLys: 5.081 ± 0.108
4.933LysLeu: 4.933 ± 0.088
1.957LysMet: 1.957 ± 0.054
2.497LysAsn: 2.497 ± 0.058
2.8LysPro: 2.8 ± 0.07
1.456LysGln: 1.456 ± 0.051
2.958LysArg: 2.958 ± 0.066
3.338LysSer: 3.338 ± 0.071
2.974LysThr: 2.974 ± 0.065
4.383LysVal: 4.383 ± 0.077
0.536LysTrp: 0.536 ± 0.025
2.423LysTyr: 2.423 ± 0.058
0.0LysXaa: 0.0 ± 0.0
Leu
8.433LeuAla: 8.433 ± 0.115
1.056LeuCys: 1.056 ± 0.039
5.508LeuAsp: 5.508 ± 0.087
5.718LeuGlu: 5.718 ± 0.095
3.552LeuPhe: 3.552 ± 0.069
6.916LeuGly: 6.916 ± 0.102
1.391LeuHis: 1.391 ± 0.039
6.16LeuIle: 6.16 ± 0.112
6.422LeuLys: 6.422 ± 0.101
8.834LeuLeu: 8.834 ± 0.135
2.703LeuMet: 2.703 ± 0.053
3.354LeuAsn: 3.354 ± 0.059
3.982LeuPro: 3.982 ± 0.074
1.983LeuGln: 1.983 ± 0.047
4.531LeuArg: 4.531 ± 0.088
6.368LeuSer: 6.368 ± 0.097
4.608LeuThr: 4.608 ± 0.085
6.496LeuVal: 6.496 ± 0.107
0.76LeuTrp: 0.76 ± 0.032
3.1LeuTyr: 3.1 ± 0.057
0.0LeuXaa: 0.0 ± 0.0
Met
3.304MetAla: 3.304 ± 0.061
0.318MetCys: 0.318 ± 0.019
1.992MetAsp: 1.992 ± 0.055
1.857MetGlu: 1.857 ± 0.048
1.034MetPhe: 1.034 ± 0.036
2.736MetGly: 2.736 ± 0.06
0.495MetHis: 0.495 ± 0.024
1.747MetIle: 1.747 ± 0.046
2.027MetLys: 2.027 ± 0.05
2.999MetLeu: 2.999 ± 0.061
0.899MetMet: 0.899 ± 0.037
1.172MetAsn: 1.172 ± 0.033
1.658MetPro: 1.658 ± 0.042
0.753MetGln: 0.753 ± 0.028
1.597MetArg: 1.597 ± 0.047
2.02MetSer: 2.02 ± 0.048
1.63MetThr: 1.63 ± 0.049
2.294MetVal: 2.294 ± 0.047
0.199MetTrp: 0.199 ± 0.014
0.872MetTyr: 0.872 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
3.008AsnAla: 3.008 ± 0.07
0.4AsnCys: 0.4 ± 0.022
2.045AsnAsp: 2.045 ± 0.057
1.974AsnGlu: 1.974 ± 0.057
1.238AsnPhe: 1.238 ± 0.04
3.151AsnGly: 3.151 ± 0.082
0.463AsnHis: 0.463 ± 0.024
2.872AsnIle: 2.872 ± 0.065
2.024AsnLys: 2.024 ± 0.051
2.764AsnLeu: 2.764 ± 0.064
1.233AsnMet: 1.233 ± 0.042
1.571AsnAsn: 1.571 ± 0.051
1.825AsnPro: 1.825 ± 0.046
0.79AsnGln: 0.79 ± 0.032
1.618AsnArg: 1.618 ± 0.043
1.969AsnSer: 1.969 ± 0.054
1.942AsnThr: 1.942 ± 0.049
3.216AsnVal: 3.216 ± 0.066
0.363AsnTrp: 0.363 ± 0.023
1.341AsnTyr: 1.341 ± 0.044
0.0AsnXaa: 0.0 ± 0.0
Pro
4.001ProAla: 4.001 ± 0.077
0.51ProCys: 0.51 ± 0.025
2.892ProAsp: 2.892 ± 0.059
3.496ProGlu: 3.496 ± 0.066
1.719ProPhe: 1.719 ± 0.049
4.1ProGly: 4.1 ± 0.077
0.72ProHis: 0.72 ± 0.029
2.506ProIle: 2.506 ± 0.058
2.1ProLys: 2.1 ± 0.06
3.843ProLeu: 3.843 ± 0.075
1.306ProMet: 1.306 ± 0.035
1.245ProAsn: 1.245 ± 0.038
1.772ProPro: 1.772 ± 0.062
1.126ProGln: 1.126 ± 0.032
1.903ProArg: 1.903 ± 0.049
2.63ProSer: 2.63 ± 0.076
2.732ProThr: 2.732 ± 0.139
3.92ProVal: 3.92 ± 0.079
0.428ProTrp: 0.428 ± 0.028
1.667ProTyr: 1.667 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
2.214GlnAla: 2.214 ± 0.06
0.282GlnCys: 0.282 ± 0.018
1.227GlnAsp: 1.227 ± 0.039
1.461GlnGlu: 1.461 ± 0.042
0.909GlnPhe: 0.909 ± 0.036
1.766GlnGly: 1.766 ± 0.053
0.428GlnHis: 0.428 ± 0.023
1.409GlnIle: 1.409 ± 0.044
1.756GlnLys: 1.756 ± 0.043
1.921GlnLeu: 1.921 ± 0.043
0.805GlnMet: 0.805 ± 0.028
0.899GlnAsn: 0.899 ± 0.034
0.953GlnPro: 0.953 ± 0.036
0.734GlnGln: 0.734 ± 0.037
1.276GlnArg: 1.276 ± 0.041
1.364GlnSer: 1.364 ± 0.044
1.124GlnThr: 1.124 ± 0.041
1.782GlnVal: 1.782 ± 0.049
0.272GlnTrp: 0.272 ± 0.018
1.009GlnTyr: 1.009 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
4.175ArgAla: 4.175 ± 0.075
0.636ArgCys: 0.636 ± 0.033
3.027ArgAsp: 3.027 ± 0.067
3.884ArgGlu: 3.884 ± 0.081
1.95ArgPhe: 1.95 ± 0.049
3.483ArgGly: 3.483 ± 0.077
0.935ArgHis: 0.935 ± 0.039
3.857ArgIle: 3.857 ± 0.08
3.425ArgLys: 3.425 ± 0.072
5.04ArgLeu: 5.04 ± 0.081
1.87ArgMet: 1.87 ± 0.048
1.848ArgAsn: 1.848 ± 0.048
2.263ArgPro: 2.263 ± 0.054
1.511ArgGln: 1.511 ± 0.041
2.951ArgArg: 2.951 ± 0.062
3.224ArgSer: 3.224 ± 0.065
2.427ArgThr: 2.427 ± 0.056
3.518ArgVal: 3.518 ± 0.067
0.485ArgTrp: 0.485 ± 0.025
1.942ArgTyr: 1.942 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
4.719SerAla: 4.719 ± 0.079
0.698SerCys: 0.698 ± 0.031
3.274SerAsp: 3.274 ± 0.07
3.263SerGlu: 3.263 ± 0.07
2.341SerPhe: 2.341 ± 0.048
5.669SerGly: 5.669 ± 0.098
1.057SerHis: 1.057 ± 0.033
4.634SerIle: 4.634 ± 0.086
3.22SerLys: 3.22 ± 0.07
5.873SerLeu: 5.873 ± 0.083
1.899SerMet: 1.899 ± 0.054
2.046SerAsn: 2.046 ± 0.063
2.992SerPro: 2.992 ± 0.079
1.559SerGln: 1.559 ± 0.051
3.135SerArg: 3.135 ± 0.064
3.867SerSer: 3.867 ± 0.092
3.27SerThr: 3.27 ± 0.08
4.45SerVal: 4.45 ± 0.071
0.668SerTrp: 0.668 ± 0.029
2.145SerTyr: 2.145 ± 0.052
0.0SerXaa: 0.0 ± 0.0
Thr
4.52ThrAla: 4.52 ± 0.086
0.598ThrCys: 0.598 ± 0.027
2.68ThrAsp: 2.68 ± 0.056
2.431ThrGlu: 2.431 ± 0.055
2.027ThrPhe: 2.027 ± 0.052
4.751ThrGly: 4.751 ± 0.073
0.935ThrHis: 0.935 ± 0.027
4.008ThrIle: 4.008 ± 0.086
2.488ThrLys: 2.488 ± 0.052
4.875ThrLeu: 4.875 ± 0.084
1.576ThrMet: 1.576 ± 0.044
1.625ThrAsn: 1.625 ± 0.048
3.558ThrPro: 3.558 ± 0.141
1.125ThrGln: 1.125 ± 0.037
2.299ThrArg: 2.299 ± 0.05
3.171ThrSer: 3.171 ± 0.074
2.714ThrThr: 2.714 ± 0.064
4.421ThrVal: 4.421 ± 0.095
0.424ThrTrp: 0.424 ± 0.02
1.843ThrTyr: 1.843 ± 0.051
0.0ThrXaa: 0.0 ± 0.0
Val
5.927ValAla: 5.927 ± 0.098
1.052ValCys: 1.052 ± 0.036
4.648ValAsp: 4.648 ± 0.068
4.974ValGlu: 4.974 ± 0.08
2.906ValPhe: 2.906 ± 0.061
5.091ValGly: 5.091 ± 0.081
1.307ValHis: 1.307 ± 0.037
5.374ValIle: 5.374 ± 0.09
4.911ValLys: 4.911 ± 0.079
7.1ValLeu: 7.1 ± 0.109
2.129ValMet: 2.129 ± 0.053
2.852ValAsn: 2.852 ± 0.056
3.826ValPro: 3.826 ± 0.074
1.7ValGln: 1.7 ± 0.047
4.033ValArg: 4.033 ± 0.074
5.024ValSer: 5.024 ± 0.09
4.543ValThr: 4.543 ± 0.08
6.026ValVal: 6.026 ± 0.096
0.729ValTrp: 0.729 ± 0.033
2.66ValTyr: 2.66 ± 0.063
0.0ValXaa: 0.0 ± 0.0
Trp
0.826TrpAla: 0.826 ± 0.032
0.142TrpCys: 0.142 ± 0.014
0.63TrpAsp: 0.63 ± 0.026
0.55TrpGlu: 0.55 ± 0.026
0.327TrpPhe: 0.327 ± 0.02
0.751TrpGly: 0.751 ± 0.036
0.178TrpHis: 0.178 ± 0.014
0.535TrpIle: 0.535 ± 0.022
0.563TrpLys: 0.563 ± 0.022
0.98TrpLeu: 0.98 ± 0.036
0.332TrpMet: 0.332 ± 0.019
0.425TrpAsn: 0.425 ± 0.025
0.325TrpPro: 0.325 ± 0.022
0.298TrpGln: 0.298 ± 0.019
0.466TrpArg: 0.466 ± 0.026
0.547TrpSer: 0.547 ± 0.027
0.418TrpThr: 0.418 ± 0.024
0.717TrpVal: 0.717 ± 0.032
0.154TrpTrp: 0.154 ± 0.016
0.381TrpTyr: 0.381 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.621TyrAla: 2.621 ± 0.057
0.521TyrCys: 0.521 ± 0.021
2.418TyrAsp: 2.418 ± 0.062
2.051TyrGlu: 2.051 ± 0.05
1.501TyrPhe: 1.501 ± 0.044
3.078TyrGly: 3.078 ± 0.071
0.727TyrHis: 0.727 ± 0.034
2.088TyrIle: 2.088 ± 0.05
1.799TyrLys: 1.799 ± 0.047
3.279TyrLeu: 3.279 ± 0.064
1.084TyrMet: 1.084 ± 0.037
1.497TyrAsn: 1.497 ± 0.052
1.686TyrPro: 1.686 ± 0.05
0.851TyrGln: 0.851 ± 0.031
2.145TyrArg: 2.145 ± 0.049
2.249TyrSer: 2.249 ± 0.05
1.984TyrThr: 1.984 ± 0.048
2.829TyrVal: 2.829 ± 0.056
0.395TyrTrp: 0.395 ± 0.026
1.605TyrTyr: 1.605 ± 0.051
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2993 proteins (852371 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski