Synechococcus phage S-CBS1
Average proteome isoelectric point is 5.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G8CLA7|G8CLA7_9CAUD Major tropism determinant OS=Synechococcus phage S-CBS1 OX=909297 GN=mtd PE=4 SV=1
MM1 pKa = 7.48 SLPRR5 pKa = 11.84 IGGFSAPGTADD16 pKa = 3.77 YY17 pKa = 11.67 ADD19 pKa = 4.59 LDD21 pKa = 4.08 YY22 pKa = 11.54 DD23 pKa = 3.98 GSDD26 pKa = 3.17 RR27 pKa = 11.84 LITITYY33 pKa = 8.17 KK34 pKa = 10.56 QGGASGGVVGTLNMTYY50 pKa = 10.23 VGEE53 pKa = 4.32 STNVDD58 pKa = 3.67 TIYY61 pKa = 10.6 WSS63 pKa = 3.21
Molecular weight: 6.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 3.986
IPC_protein 3.834
Toseland 3.63
ProMoST 4.062
Dawson 3.872
Bjellqvist 4.037
Wikipedia 3.884
Rodwell 3.681
Grimsley 3.554
Solomon 3.834
Lehninger 3.795
Nozaki 4.024
DTASelect 4.291
Thurlkill 3.732
EMBOSS 3.884
Sillero 3.973
Patrickios 0.604
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.869
Protein with the highest isoelectric point:
>tr|G8CLE3|G8CLE3_9CAUD Uncharacterized protein OS=Synechococcus phage S-CBS1 OX=909297 GN=S-CBS1_gp43 PE=4 SV=1
MM1 pKa = 7.44 LAIKK5 pKa = 10.54 LIDD8 pKa = 4.55 NIEE11 pKa = 3.75 KK12 pKa = 9.28 TAIKK16 pKa = 10.2 RR17 pKa = 11.84 VTNMQKK23 pKa = 10.45 QMAFASSVALNEE35 pKa = 3.89 TAFQARR41 pKa = 11.84 QSLNKK46 pKa = 9.7 STVQFFNAPVKK57 pKa = 9.0 FTQSAFLVEE66 pKa = 4.55 KK67 pKa = 10.56 SKK69 pKa = 9.42 KK70 pKa = 7.64 TNLIATVYY78 pKa = 10.5 AQDD81 pKa = 3.42 AAGKK85 pKa = 10.11 DD86 pKa = 3.04 RR87 pKa = 11.84 ARR89 pKa = 11.84 YY90 pKa = 9.51 LRR92 pKa = 11.84 FGTAGGTRR100 pKa = 11.84 PQKK103 pKa = 9.89 GFEE106 pKa = 4.18 RR107 pKa = 11.84 FFSGAIPSDD116 pKa = 3.46 GTIPPGSYY124 pKa = 10.73 FMPTSTVKK132 pKa = 9.95 TNRR135 pKa = 11.84 HH136 pKa = 5.39 GNVSQATLRR145 pKa = 11.84 RR146 pKa = 11.84 ITKK149 pKa = 9.87 GINGDD154 pKa = 3.34 PRR156 pKa = 11.84 GGFFMGTPRR165 pKa = 11.84 GGNRR169 pKa = 11.84 PPGIYY174 pKa = 9.47 RR175 pKa = 11.84 RR176 pKa = 11.84 SRR178 pKa = 11.84 LRR180 pKa = 11.84 LTPYY184 pKa = 10.68 FIATQDD190 pKa = 3.56 KK191 pKa = 9.68 PDD193 pKa = 3.42 YY194 pKa = 8.67 TARR197 pKa = 11.84 FPISTITQKK206 pKa = 10.22 VVQRR210 pKa = 11.84 SYY212 pKa = 11.49 SGNFQTALAKK222 pKa = 10.63 ALATARR228 pKa = 3.91
Molecular weight: 25.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.261
IPC2_protein 10.175
IPC_protein 11.155
Toseland 11.286
ProMoST 11.52
Dawson 11.33
Bjellqvist 11.155
Wikipedia 11.652
Rodwell 11.462
Grimsley 11.359
Solomon 11.623
Lehninger 11.55
Nozaki 11.257
DTASelect 11.155
Thurlkill 11.272
EMBOSS 11.725
Sillero 11.286
Patrickios 11.169
IPC_peptide 11.623
IPC2_peptide 10.394
IPC2.peptide.svr19 8.553
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
43
0
43
9374
40
836
218.0
23.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.529 ± 0.552
1.056 ± 0.173
6.198 ± 0.279
6.113 ± 0.393
2.998 ± 0.252
8.161 ± 0.425
1.675 ± 0.234
4.918 ± 0.325
3.339 ± 0.258
8.47 ± 0.403
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.24 ± 0.219
3.563 ± 0.197
5.163 ± 0.351
4.011 ± 0.235
6.337 ± 0.442
6.667 ± 0.308
7.094 ± 0.488
6.742 ± 0.278
1.856 ± 0.203
2.87 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here