Amino acid dipepetide frequency for Synechococcus phage S-CBS1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.55AlaAla: 13.55 ± 1.675
1.067AlaCys: 1.067 ± 0.433
6.935AlaAsp: 6.935 ± 0.895
6.188AlaGlu: 6.188 ± 0.746
3.948AlaPhe: 3.948 ± 0.983
8.642AlaGly: 8.642 ± 1.372
0.533AlaHis: 0.533 ± 0.355
5.761AlaIle: 5.761 ± 0.815
3.307AlaLys: 3.307 ± 0.603
7.468AlaLeu: 7.468 ± 1.088
2.134AlaMet: 2.134 ± 0.438
3.734AlaAsn: 3.734 ± 0.607
5.868AlaPro: 5.868 ± 0.633
5.014AlaGln: 5.014 ± 0.699
4.801AlaArg: 4.801 ± 0.899
6.188AlaSer: 6.188 ± 0.946
7.895AlaThr: 7.895 ± 0.872
8.108AlaVal: 8.108 ± 0.863
1.6AlaTrp: 1.6 ± 0.506
2.561AlaTyr: 2.561 ± 0.455
0.0AlaXaa: 0.0 ± 0.0
Cys
0.533CysAla: 0.533 ± 0.31
0.32CysCys: 0.32 ± 0.166
0.747CysAsp: 0.747 ± 0.274
0.854CysGlu: 0.854 ± 0.42
0.427CysPhe: 0.427 ± 0.225
1.174CysGly: 1.174 ± 0.415
0.213CysHis: 0.213 ± 0.222
0.32CysIle: 0.32 ± 0.186
0.533CysLys: 0.533 ± 0.208
0.64CysLeu: 0.64 ± 0.213
0.32CysMet: 0.32 ± 0.182
0.0CysAsn: 0.0 ± 0.0
0.533CysPro: 0.533 ± 0.213
0.107CysGln: 0.107 ± 0.11
1.707CysArg: 1.707 ± 0.399
1.067CysSer: 1.067 ± 0.322
0.107CysThr: 0.107 ± 0.11
0.854CysVal: 0.854 ± 0.317
0.107CysTrp: 0.107 ± 0.11
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
6.081AspAla: 6.081 ± 0.712
0.32AspCys: 0.32 ± 0.25
3.734AspAsp: 3.734 ± 0.627
3.841AspGlu: 3.841 ± 0.749
1.707AspPhe: 1.707 ± 0.422
6.828AspGly: 6.828 ± 1.146
1.174AspHis: 1.174 ± 0.356
1.814AspIle: 1.814 ± 0.542
2.027AspLys: 2.027 ± 0.433
6.188AspLeu: 6.188 ± 0.622
1.92AspMet: 1.92 ± 0.521
2.134AspAsn: 2.134 ± 0.553
3.521AspPro: 3.521 ± 0.606
2.561AspGln: 2.561 ± 0.599
2.987AspArg: 2.987 ± 0.492
3.307AspSer: 3.307 ± 0.558
4.268AspThr: 4.268 ± 0.757
3.734AspVal: 3.734 ± 0.614
1.494AspTrp: 1.494 ± 0.498
2.347AspTyr: 2.347 ± 0.494
0.0AspXaa: 0.0 ± 0.0
Glu
7.042GluAla: 7.042 ± 0.889
0.64GluCys: 0.64 ± 0.31
2.561GluAsp: 2.561 ± 0.524
2.774GluGlu: 2.774 ± 0.582
1.92GluPhe: 1.92 ± 0.462
4.268GluGly: 4.268 ± 0.74
1.6GluHis: 1.6 ± 0.389
4.054GluIle: 4.054 ± 0.733
2.881GluLys: 2.881 ± 0.668
5.761GluLeu: 5.761 ± 0.967
0.747GluMet: 0.747 ± 0.251
1.6GluAsn: 1.6 ± 0.376
3.734GluPro: 3.734 ± 0.491
3.201GluGln: 3.201 ± 0.5
4.161GluArg: 4.161 ± 0.691
4.161GluSer: 4.161 ± 0.619
3.307GluThr: 3.307 ± 0.793
4.694GluVal: 4.694 ± 0.912
0.747GluTrp: 0.747 ± 0.32
1.28GluTyr: 1.28 ± 0.494
0.0GluXaa: 0.0 ± 0.0
Phe
2.454PheAla: 2.454 ± 0.764
0.64PheCys: 0.64 ± 0.266
2.027PheAsp: 2.027 ± 0.462
2.134PheGlu: 2.134 ± 0.565
0.64PhePhe: 0.64 ± 0.351
2.561PheGly: 2.561 ± 0.462
0.854PheHis: 0.854 ± 0.409
1.494PheIle: 1.494 ± 0.346
0.96PheLys: 0.96 ± 0.309
1.6PheLeu: 1.6 ± 0.391
1.067PheMet: 1.067 ± 0.3
0.854PheAsn: 0.854 ± 0.368
1.814PhePro: 1.814 ± 0.489
0.854PheGln: 0.854 ± 0.284
1.814PheArg: 1.814 ± 0.493
2.454PheSer: 2.454 ± 0.518
3.307PheThr: 3.307 ± 0.675
1.6PheVal: 1.6 ± 0.452
0.32PheTrp: 0.32 ± 0.172
0.533PheTyr: 0.533 ± 0.232
0.0PheXaa: 0.0 ± 0.0
Gly
7.575GlyAla: 7.575 ± 1.251
0.64GlyCys: 0.64 ± 0.342
5.334GlyAsp: 5.334 ± 0.611
3.627GlyGlu: 3.627 ± 0.536
2.561GlyPhe: 2.561 ± 0.482
8.322GlyGly: 8.322 ± 1.992
1.707GlyHis: 1.707 ± 0.469
3.948GlyIle: 3.948 ± 0.629
2.987GlyLys: 2.987 ± 0.746
6.188GlyLeu: 6.188 ± 0.902
1.814GlyMet: 1.814 ± 0.466
3.414GlyAsn: 3.414 ± 0.633
2.987GlyPro: 2.987 ± 0.812
2.667GlyGln: 2.667 ± 0.607
4.801GlyArg: 4.801 ± 0.575
7.042GlySer: 7.042 ± 1.286
6.828GlyThr: 6.828 ± 1.033
5.441GlyVal: 5.441 ± 0.705
2.027GlyTrp: 2.027 ± 0.584
1.707GlyTyr: 1.707 ± 0.553
0.0GlyXaa: 0.0 ± 0.0
His
1.494HisAla: 1.494 ± 0.468
0.533HisCys: 0.533 ± 0.269
0.533HisAsp: 0.533 ± 0.211
0.96HisGlu: 0.96 ± 0.308
0.533HisPhe: 0.533 ± 0.232
1.174HisGly: 1.174 ± 0.412
0.427HisHis: 0.427 ± 0.186
0.427HisIle: 0.427 ± 0.208
0.64HisLys: 0.64 ± 0.234
2.027HisLeu: 2.027 ± 0.478
0.32HisMet: 0.32 ± 0.163
0.64HisAsn: 0.64 ± 0.228
0.747HisPro: 0.747 ± 0.218
1.067HisGln: 1.067 ± 0.364
1.387HisArg: 1.387 ± 0.402
0.64HisSer: 0.64 ± 0.275
0.747HisThr: 0.747 ± 0.28
1.067HisVal: 1.067 ± 0.455
0.427HisTrp: 0.427 ± 0.209
0.96HisTyr: 0.96 ± 0.326
0.0HisXaa: 0.0 ± 0.0
Ile
5.121IleAla: 5.121 ± 0.821
0.213IleCys: 0.213 ± 0.143
4.694IleAsp: 4.694 ± 0.821
5.121IleGlu: 5.121 ± 0.82
0.96IlePhe: 0.96 ± 0.522
4.161IleGly: 4.161 ± 0.733
0.747IleHis: 0.747 ± 0.257
1.92IleIle: 1.92 ± 0.465
1.28IleLys: 1.28 ± 0.332
3.307IleLeu: 3.307 ± 0.572
0.96IleMet: 0.96 ± 0.321
1.814IleAsn: 1.814 ± 0.348
2.347IlePro: 2.347 ± 0.44
1.494IleGln: 1.494 ± 0.512
2.774IleArg: 2.774 ± 0.448
3.094IleSer: 3.094 ± 0.595
3.627IleThr: 3.627 ± 0.517
3.094IleVal: 3.094 ± 0.681
0.747IleTrp: 0.747 ± 0.43
1.707IleTyr: 1.707 ± 0.458
0.0IleXaa: 0.0 ± 0.0
Lys
3.627LysAla: 3.627 ± 0.542
0.427LysCys: 0.427 ± 0.214
1.6LysAsp: 1.6 ± 0.443
2.027LysGlu: 2.027 ± 0.481
1.067LysPhe: 1.067 ± 0.258
2.454LysGly: 2.454 ± 0.881
0.107LysHis: 0.107 ± 0.118
1.6LysIle: 1.6 ± 0.357
1.28LysLys: 1.28 ± 0.345
3.307LysLeu: 3.307 ± 0.925
0.747LysMet: 0.747 ± 0.258
1.174LysAsn: 1.174 ± 0.365
2.347LysPro: 2.347 ± 0.561
1.494LysGln: 1.494 ± 0.335
3.948LysArg: 3.948 ± 0.906
1.387LysSer: 1.387 ± 0.376
2.347LysThr: 2.347 ± 0.645
1.707LysVal: 1.707 ± 0.314
0.213LysTrp: 0.213 ± 0.149
0.533LysTyr: 0.533 ± 0.226
0.0LysXaa: 0.0 ± 0.0
Leu
7.362LeuAla: 7.362 ± 0.877
0.854LeuCys: 0.854 ± 0.293
5.868LeuAsp: 5.868 ± 0.867
5.975LeuGlu: 5.975 ± 0.852
1.6LeuPhe: 1.6 ± 0.272
5.014LeuGly: 5.014 ± 0.758
2.027LeuHis: 2.027 ± 0.45
5.228LeuIle: 5.228 ± 0.956
3.734LeuLys: 3.734 ± 0.729
7.362LeuLeu: 7.362 ± 1.037
2.667LeuMet: 2.667 ± 0.571
4.054LeuAsn: 4.054 ± 0.658
3.201LeuPro: 3.201 ± 0.641
3.627LeuGln: 3.627 ± 0.602
5.975LeuArg: 5.975 ± 0.992
5.548LeuSer: 5.548 ± 0.641
5.548LeuThr: 5.548 ± 0.695
6.188LeuVal: 6.188 ± 0.657
0.96LeuTrp: 0.96 ± 0.283
1.92LeuTyr: 1.92 ± 0.569
0.0LeuXaa: 0.0 ± 0.0
Met
3.307MetAla: 3.307 ± 0.641
0.107MetCys: 0.107 ± 0.1
1.387MetAsp: 1.387 ± 0.387
1.174MetGlu: 1.174 ± 0.444
0.427MetPhe: 0.427 ± 0.264
1.494MetGly: 1.494 ± 0.519
0.213MetHis: 0.213 ± 0.171
1.067MetIle: 1.067 ± 0.336
0.747MetLys: 0.747 ± 0.324
1.92MetLeu: 1.92 ± 0.369
0.32MetMet: 0.32 ± 0.163
0.32MetAsn: 0.32 ± 0.166
2.134MetPro: 2.134 ± 0.456
0.747MetGln: 0.747 ± 0.277
1.174MetArg: 1.174 ± 0.325
1.28MetSer: 1.28 ± 0.308
2.454MetThr: 2.454 ± 0.487
1.387MetVal: 1.387 ± 0.461
0.533MetTrp: 0.533 ± 0.263
0.213MetTyr: 0.213 ± 0.135
0.0MetXaa: 0.0 ± 0.0
Asn
3.201AsnAla: 3.201 ± 0.654
0.107AsnCys: 0.107 ± 0.102
2.987AsnAsp: 2.987 ± 0.486
1.814AsnGlu: 1.814 ± 0.387
0.854AsnPhe: 0.854 ± 0.34
3.948AsnGly: 3.948 ± 0.605
0.747AsnHis: 0.747 ± 0.281
0.854AsnIle: 0.854 ± 0.381
1.174AsnLys: 1.174 ± 0.387
2.24AsnLeu: 2.24 ± 0.385
1.28AsnMet: 1.28 ± 0.376
1.387AsnAsn: 1.387 ± 0.539
1.92AsnPro: 1.92 ± 0.465
0.854AsnGln: 0.854 ± 0.299
2.134AsnArg: 2.134 ± 0.59
2.667AsnSer: 2.667 ± 0.548
2.987AsnThr: 2.987 ± 0.574
2.134AsnVal: 2.134 ± 0.453
1.174AsnTrp: 1.174 ± 0.397
1.174AsnTyr: 1.174 ± 0.419
0.0AsnXaa: 0.0 ± 0.0
Pro
6.615ProAla: 6.615 ± 0.887
0.64ProCys: 0.64 ± 0.231
3.734ProAsp: 3.734 ± 0.711
2.987ProGlu: 2.987 ± 0.446
1.707ProPhe: 1.707 ± 0.496
4.268ProGly: 4.268 ± 0.69
0.32ProHis: 0.32 ± 0.237
2.667ProIle: 2.667 ± 0.522
0.64ProLys: 0.64 ± 0.273
4.268ProLeu: 4.268 ± 0.741
1.174ProMet: 1.174 ± 0.355
1.067ProAsn: 1.067 ± 0.391
2.347ProPro: 2.347 ± 0.611
1.814ProGln: 1.814 ± 0.479
2.987ProArg: 2.987 ± 0.564
3.201ProSer: 3.201 ± 0.818
4.161ProThr: 4.161 ± 0.8
3.734ProVal: 3.734 ± 0.625
1.28ProTrp: 1.28 ± 0.284
2.027ProTyr: 2.027 ± 0.395
0.0ProXaa: 0.0 ± 0.0
Gln
4.908GlnAla: 4.908 ± 0.787
0.32GlnCys: 0.32 ± 0.186
1.814GlnAsp: 1.814 ± 0.359
2.027GlnGlu: 2.027 ± 0.638
0.533GlnPhe: 0.533 ± 0.288
3.521GlnGly: 3.521 ± 0.633
0.533GlnHis: 0.533 ± 0.293
2.24GlnIle: 2.24 ± 0.641
1.174GlnLys: 1.174 ± 0.473
4.161GlnLeu: 4.161 ± 0.9
1.28GlnMet: 1.28 ± 0.384
0.854GlnAsn: 0.854 ± 0.299
3.201GlnPro: 3.201 ± 0.765
2.24GlnGln: 2.24 ± 0.649
2.881GlnArg: 2.881 ± 0.603
3.094GlnSer: 3.094 ± 0.583
1.6GlnThr: 1.6 ± 0.373
2.347GlnVal: 2.347 ± 0.464
0.854GlnTrp: 0.854 ± 0.334
0.533GlnTyr: 0.533 ± 0.283
0.0GlnXaa: 0.0 ± 0.0
Arg
7.042ArgAla: 7.042 ± 0.974
0.533ArgCys: 0.533 ± 0.239
2.774ArgAsp: 2.774 ± 0.599
4.161ArgGlu: 4.161 ± 0.958
2.881ArgPhe: 2.881 ± 0.546
3.734ArgGly: 3.734 ± 0.701
1.6ArgHis: 1.6 ± 0.4
3.307ArgIle: 3.307 ± 0.558
1.6ArgLys: 1.6 ± 0.424
6.508ArgLeu: 6.508 ± 0.972
1.494ArgMet: 1.494 ± 0.507
2.134ArgAsn: 2.134 ± 0.416
2.454ArgPro: 2.454 ± 0.543
2.881ArgGln: 2.881 ± 0.554
4.694ArgArg: 4.694 ± 0.613
4.694ArgSer: 4.694 ± 0.732
2.561ArgThr: 2.561 ± 0.537
4.161ArgVal: 4.161 ± 0.54
1.6ArgTrp: 1.6 ± 0.378
2.454ArgTyr: 2.454 ± 0.544
0.0ArgXaa: 0.0 ± 0.0
Ser
5.228SerAla: 5.228 ± 0.58
0.64SerCys: 0.64 ± 0.371
4.161SerAsp: 4.161 ± 0.632
3.201SerGlu: 3.201 ± 0.637
3.201SerPhe: 3.201 ± 0.661
5.975SerGly: 5.975 ± 1.041
1.174SerHis: 1.174 ± 0.42
2.881SerIle: 2.881 ± 0.627
2.561SerLys: 2.561 ± 0.602
6.188SerLeu: 6.188 ± 0.954
1.387SerMet: 1.387 ± 0.299
2.454SerAsn: 2.454 ± 0.411
3.841SerPro: 3.841 ± 0.898
2.561SerGln: 2.561 ± 0.379
4.481SerArg: 4.481 ± 0.867
3.734SerSer: 3.734 ± 0.72
5.228SerThr: 5.228 ± 0.815
3.841SerVal: 3.841 ± 0.616
1.174SerTrp: 1.174 ± 0.433
2.774SerTyr: 2.774 ± 0.541
0.0SerXaa: 0.0 ± 0.0
Thr
8.642ThrAla: 8.642 ± 1.38
0.854ThrCys: 0.854 ± 0.426
4.694ThrAsp: 4.694 ± 0.826
3.627ThrGlu: 3.627 ± 0.829
1.92ThrPhe: 1.92 ± 0.527
5.121ThrGly: 5.121 ± 0.852
1.174ThrHis: 1.174 ± 0.401
3.521ThrIle: 3.521 ± 0.581
1.707ThrLys: 1.707 ± 0.394
6.081ThrLeu: 6.081 ± 1.026
1.707ThrMet: 1.707 ± 0.445
3.094ThrAsn: 3.094 ± 0.785
3.734ThrPro: 3.734 ± 0.65
2.24ThrGln: 2.24 ± 0.522
2.24ThrArg: 2.24 ± 0.551
5.121ThrSer: 5.121 ± 0.608
6.401ThrThr: 6.401 ± 1.249
5.121ThrVal: 5.121 ± 0.751
1.6ThrTrp: 1.6 ± 0.484
2.347ThrTyr: 2.347 ± 0.456
0.0ThrXaa: 0.0 ± 0.0
Val
6.935ValAla: 6.935 ± 1.192
0.64ValCys: 0.64 ± 0.275
3.734ValAsp: 3.734 ± 0.602
4.588ValGlu: 4.588 ± 0.693
1.814ValPhe: 1.814 ± 0.383
4.694ValGly: 4.694 ± 0.743
0.64ValHis: 0.64 ± 0.319
3.841ValIle: 3.841 ± 0.585
2.347ValLys: 2.347 ± 0.481
5.121ValLeu: 5.121 ± 0.974
0.32ValMet: 0.32 ± 0.231
3.414ValAsn: 3.414 ± 0.435
3.307ValPro: 3.307 ± 0.474
2.561ValGln: 2.561 ± 0.463
5.014ValArg: 5.014 ± 0.937
5.548ValSer: 5.548 ± 0.746
5.334ValThr: 5.334 ± 0.815
5.014ValVal: 5.014 ± 0.612
0.64ValTrp: 0.64 ± 0.336
1.92ValTyr: 1.92 ± 0.507
0.0ValXaa: 0.0 ± 0.0
Trp
1.707TrpAla: 1.707 ± 0.467
0.427TrpCys: 0.427 ± 0.221
0.96TrpAsp: 0.96 ± 0.348
1.28TrpGlu: 1.28 ± 0.581
0.107TrpPhe: 0.107 ± 0.099
1.387TrpGly: 1.387 ± 0.324
0.533TrpHis: 0.533 ± 0.252
0.854TrpIle: 0.854 ± 0.319
0.64TrpLys: 0.64 ± 0.243
1.707TrpLeu: 1.707 ± 0.43
0.533TrpMet: 0.533 ± 0.258
0.854TrpAsn: 0.854 ± 0.249
0.64TrpPro: 0.64 ± 0.353
0.96TrpGln: 0.96 ± 0.353
1.6TrpArg: 1.6 ± 0.477
0.747TrpSer: 0.747 ± 0.297
1.174TrpThr: 1.174 ± 0.462
1.174TrpVal: 1.174 ± 0.353
0.427TrpTrp: 0.427 ± 0.219
0.854TrpTyr: 0.854 ± 0.323
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.881TyrAla: 2.881 ± 0.573
0.64TyrCys: 0.64 ± 0.2
1.707TyrAsp: 1.707 ± 0.495
2.774TyrGlu: 2.774 ± 0.742
1.174TyrPhe: 1.174 ± 0.351
2.881TyrGly: 2.881 ± 0.539
0.427TyrHis: 0.427 ± 0.24
1.387TyrIle: 1.387 ± 0.423
1.174TyrLys: 1.174 ± 0.347
2.667TyrLeu: 2.667 ± 0.44
0.107TyrMet: 0.107 ± 0.096
0.64TyrAsn: 0.64 ± 0.28
0.96TyrPro: 0.96 ± 0.371
1.174TyrGln: 1.174 ± 0.474
1.814TyrArg: 1.814 ± 0.458
1.707TyrSer: 1.707 ± 0.457
1.067TyrThr: 1.067 ± 0.304
2.027TyrVal: 2.027 ± 0.489
0.64TyrTrp: 0.64 ± 0.251
0.854TyrTyr: 0.854 ± 0.412
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 43 proteins (9374 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski