Prunus persica (Peach) (Amygdalus persica)
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 38732 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A251NXL8|A0A251NXL8_PRUPE Isoform of A0A251NXL2 NPH3 domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_6G296800 PE=3 SV=1
MM1 pKa = 7.57 EE2 pKa = 4.43 NHH4 pKa = 6.05 NAKK7 pKa = 10.28 LGSGGGLKK15 pKa = 10.39 PNNNNSISEE24 pKa = 4.06 QEE26 pKa = 3.93 QNPNLNQCEE35 pKa = 4.14 EE36 pKa = 4.23 QEE38 pKa = 4.29 QEE40 pKa = 4.1 VTTEE44 pKa = 3.87 SSQLVDD50 pKa = 3.92 PPSPPVPQSSRR61 pKa = 11.84 TPFTNLSQVDD71 pKa = 3.56 ADD73 pKa = 3.99 LALARR78 pKa = 11.84 TLQEE82 pKa = 3.58 QEE84 pKa = 3.59 RR85 pKa = 11.84 AYY87 pKa = 11.09 MMLRR91 pKa = 11.84 MNNEE95 pKa = 3.83 DD96 pKa = 3.17 SDD98 pKa = 4.3 YY99 pKa = 11.26 GSWEE103 pKa = 3.81 AGSYY107 pKa = 6.97 VHH109 pKa = 7.27 EE110 pKa = 5.72 DD111 pKa = 3.22 EE112 pKa = 6.65 DD113 pKa = 5.4 DD114 pKa = 4.37 FNDD117 pKa = 4.01 FHH119 pKa = 8.34 DD120 pKa = 4.86 DD121 pKa = 3.4 TDD123 pKa = 5.0 DD124 pKa = 4.54 NDD126 pKa = 5.73 DD127 pKa = 3.62 EE128 pKa = 4.27 EE129 pKa = 5.74 QYY131 pKa = 11.63 DD132 pKa = 3.93 GTEE135 pKa = 3.66 VDD137 pKa = 3.46 NDD139 pKa = 2.84 EE140 pKa = 5.43 DD141 pKa = 4.14 AFDD144 pKa = 3.82 VHH146 pKa = 7.16 GHH148 pKa = 6.96 DD149 pKa = 6.06 DD150 pKa = 4.18 AGEE153 pKa = 4.35 DD154 pKa = 3.49 DD155 pKa = 4.39 NPRR158 pKa = 11.84 VEE160 pKa = 4.16 YY161 pKa = 10.75 DD162 pKa = 3.43 PAVFSSDD169 pKa = 2.91 EE170 pKa = 4.06 AYY172 pKa = 10.58 ARR174 pKa = 11.84 ALQDD178 pKa = 3.52 AEE180 pKa = 4.24 DD181 pKa = 4.41 RR182 pKa = 11.84 EE183 pKa = 4.39 MAARR187 pKa = 11.84 LMALAGINDD196 pKa = 3.98 LEE198 pKa = 4.34 HH199 pKa = 6.34 TEE201 pKa = 3.98 EE202 pKa = 4.99 HH203 pKa = 6.66 GGNSQDD209 pKa = 2.71 TWEE212 pKa = 4.48 EE213 pKa = 3.7 VDD215 pKa = 4.04 PDD217 pKa = 3.66 EE218 pKa = 5.85 LSYY221 pKa = 11.74 EE222 pKa = 4.03 EE223 pKa = 5.69 LIALGEE229 pKa = 4.38 VVGTEE234 pKa = 4.12 SRR236 pKa = 11.84 GLSADD241 pKa = 4.28 TIASLPSVSYY251 pKa = 8.74 KK252 pKa = 10.24 TGSGQNGSNEE262 pKa = 4.06 SCVICRR268 pKa = 11.84 LDD270 pKa = 3.56 YY271 pKa = 10.92 EE272 pKa = 4.51 AGEE275 pKa = 4.19 NLTILSCKK283 pKa = 9.87 HH284 pKa = 6.4 SYY286 pKa = 10.34 HH287 pKa = 6.98 SEE289 pKa = 4.29 CINNWLTINKK299 pKa = 8.07 VCPVCSAEE307 pKa = 4.14 VSTSGNSS314 pKa = 3.0
Molecular weight: 34.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.708
IPC2_protein 3.821
IPC_protein 3.821
Toseland 3.63
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.694
Rodwell 3.656
Grimsley 3.528
Solomon 3.795
Lehninger 3.745
Nozaki 3.91
DTASelect 4.101
Thurlkill 3.656
EMBOSS 3.706
Sillero 3.948
Patrickios 1.354
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.829
Protein with the highest isoelectric point:
>tr|A0A251NR89|A0A251NR89_PRUPE Protein kinase domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_6G161700 PE=4 SV=1
MM1 pKa = 7.99 RR2 pKa = 11.84 SCQSLGHH9 pKa = 6.3 KK10 pKa = 9.88 RR11 pKa = 11.84 RR12 pKa = 11.84 STRR15 pKa = 11.84 TGFSSSPQNLPSALRR30 pKa = 11.84 MTTPSQTNLHH40 pKa = 6.71 RR41 pKa = 11.84 ILPPSAIKK49 pKa = 10.27 IIINNTRR56 pKa = 11.84 HH57 pKa = 6.21 RR58 pKa = 11.84 ATPSRR63 pKa = 11.84 RR64 pKa = 11.84 AKK66 pKa = 9.95 RR67 pKa = 11.84 RR68 pKa = 11.84 HH69 pKa = 5.33 GLLTQSRR76 pKa = 11.84 RR77 pKa = 11.84 RR78 pKa = 11.84 GTLLLL83 pKa = 4.29
Molecular weight: 9.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.479
IPC2_protein 11.052
IPC_protein 12.647
Toseland 12.822
ProMoST 13.32
Dawson 12.822
Bjellqvist 12.808
Wikipedia 13.29
Rodwell 12.398
Grimsley 12.852
Solomon 13.32
Lehninger 13.217
Nozaki 12.822
DTASelect 12.808
Thurlkill 12.822
EMBOSS 13.32
Sillero 12.822
Patrickios 12.135
IPC_peptide 13.32
IPC2_peptide 12.31
IPC2.peptide.svr19 9.136
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
26847
11885
38732
17259961
29
5363
445.6
49.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.645 ± 0.011
1.909 ± 0.006
5.281 ± 0.008
6.459 ± 0.013
4.202 ± 0.009
6.498 ± 0.011
2.465 ± 0.005
5.249 ± 0.01
6.094 ± 0.011
9.905 ± 0.016
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.367 ± 0.004
4.479 ± 0.008
4.881 ± 0.01
3.824 ± 0.011
5.221 ± 0.008
9.081 ± 0.014
4.856 ± 0.007
6.519 ± 0.01
1.289 ± 0.005
2.777 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here