Bovine group B rotavirus
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6KF86|Q6KF86_9REOV Outer capsid glycoprotein VP7 OS=Bovine group B rotavirus OX=35334 GN=VP7 PE=3 SV=1
MM1 pKa = 8.02 PLPLLLVLAACAKK14 pKa = 9.3 AQLIITPISNPEE26 pKa = 3.61 ICVLHH31 pKa = 6.92 ANDD34 pKa = 4.38 RR35 pKa = 11.84 NVNTFGEE42 pKa = 4.27 NFTNIFEE49 pKa = 4.87 SYY51 pKa = 11.2 NSVTLSFYY59 pKa = 11.1 NYY61 pKa = 10.31 DD62 pKa = 3.57 SNNYY66 pKa = 9.78 DD67 pKa = 3.8 VIDD70 pKa = 3.82 ILSKK74 pKa = 10.58 KK75 pKa = 10.31 DD76 pKa = 3.47 YY77 pKa = 10.63 SLCQILAIDD86 pKa = 4.34 VIRR89 pKa = 11.84 PEE91 pKa = 3.68 MDD93 pKa = 4.33 FITFLQSNNEE103 pKa = 3.75 CSKK106 pKa = 10.69 YY107 pKa = 10.16 SRR109 pKa = 11.84 QKK111 pKa = 9.79 IHH113 pKa = 5.52 YY114 pKa = 8.27 QKK116 pKa = 10.11 LTTGDD121 pKa = 2.86 EE122 pKa = 3.97 WFVYY126 pKa = 10.29 SKK128 pKa = 11.0 NLKK131 pKa = 9.78 FCPLSDD137 pKa = 3.76 NLIGLYY143 pKa = 10.43 CDD145 pKa = 3.46 TQVNGTYY152 pKa = 10.25 FPLSNNEE159 pKa = 4.16 RR160 pKa = 11.84 YY161 pKa = 10.04 DD162 pKa = 3.63 VTDD165 pKa = 3.66 LPEE168 pKa = 4.25 FTEE171 pKa = 3.96 MGYY174 pKa = 10.53 VFYY177 pKa = 10.98 SDD179 pKa = 4.25 DD180 pKa = 4.04 DD181 pKa = 4.4 FYY183 pKa = 10.89 ICKK186 pKa = 10.14 RR187 pKa = 11.84 VTEE190 pKa = 4.36 EE191 pKa = 3.11 KK192 pKa = 9.67 WINYY196 pKa = 8.41 HH197 pKa = 6.33 LFYY200 pKa = 10.77 RR201 pKa = 11.84 EE202 pKa = 3.82 YY203 pKa = 10.22 SASGTVARR211 pKa = 11.84 AISWDD216 pKa = 3.87 NVWTGFKK223 pKa = 10.06 TFAQVVYY230 pKa = 10.17 KK231 pKa = 10.64 ILDD234 pKa = 3.15 IFFNNRR240 pKa = 11.84 RR241 pKa = 11.84 NFEE244 pKa = 3.84 PRR246 pKa = 11.84 AA247 pKa = 3.56
Molecular weight: 28.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.732
IPC2_protein 4.902
IPC_protein 4.813
Toseland 4.673
ProMoST 4.94
Dawson 4.787
Bjellqvist 4.927
Wikipedia 4.673
Rodwell 4.673
Grimsley 4.596
Solomon 4.787
Lehninger 4.736
Nozaki 4.902
DTASelect 5.092
Thurlkill 4.685
EMBOSS 4.698
Sillero 4.952
Patrickios 1.113
IPC_peptide 4.787
IPC2_peptide 4.94
IPC2.peptide.svr19 4.87
Protein with the highest isoelectric point:
>tr|Q6JPH2|Q6JPH2_9REOV Nonstructural protein 5 OS=Bovine group B rotavirus OX=35334 GN=NSP5 PE=4 SV=1
MM1 pKa = 7.53 GSSQSSLQTQVHH13 pKa = 5.25 STNIHH18 pKa = 5.07 SQHH21 pKa = 6.8 SSIHH25 pKa = 5.35 LQGTSTATFTTHH37 pKa = 6.73 QIIITAGAVLIALLLTSLVFSCICNCYY64 pKa = 10.24 LYY66 pKa = 11.09 SKK68 pKa = 9.9 VKK70 pKa = 10.8 NGFQTIPLQFKK81 pKa = 9.72 RR82 pKa = 11.84 KK83 pKa = 8.59 EE84 pKa = 4.19 RR85 pKa = 11.84 FDD87 pKa = 3.71 SSIPQPRR94 pKa = 11.84 IQPTQFVV101 pKa = 3.57
Molecular weight: 11.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.171
IPC2_protein 8.858
IPC_protein 8.916
Toseland 9.443
ProMoST 9.253
Dawson 9.706
Bjellqvist 9.575
Wikipedia 9.853
Rodwell 9.97
Grimsley 9.736
Solomon 9.838
Lehninger 9.823
Nozaki 9.794
DTASelect 9.458
Thurlkill 9.589
EMBOSS 9.867
Sillero 9.75
Patrickios 7.483
IPC_peptide 9.838
IPC2_peptide 8.726
IPC2.peptide.svr19 7.778
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
4698
101
1158
469.8
53.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.13 ± 0.412
1.596 ± 0.571
5.811 ± 0.562
5.449 ± 0.358
4.47 ± 0.473
3.853 ± 0.449
1.724 ± 0.287
7.642 ± 0.314
6.301 ± 0.74
8.365 ± 0.556
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.341 ± 0.15
6.003 ± 0.376
4.172 ± 0.349
3.917 ± 0.451
5.194 ± 0.399
8.472 ± 0.38
6.982 ± 0.498
6.599 ± 0.502
1.022 ± 0.158
3.959 ± 0.486
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here