Actinomadura sp. LHW52907

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Actinomadura; unclassified Actinomadura

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6669 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A372G8P6|A0A372G8P6_9ACTN Histidine kinase OS=Actinomadura sp. LHW52907 OX=2303421 GN=D0T12_30970 PE=4 SV=1
MM1 pKa = 7.24TSYY4 pKa = 10.71IVVFGLIVVVVLVVVLVALGLRR26 pKa = 11.84ASRR29 pKa = 11.84AGRR32 pKa = 11.84DD33 pKa = 3.44DD34 pKa = 5.19DD35 pKa = 5.62DD36 pKa = 4.1WMDD39 pKa = 5.69DD40 pKa = 3.5EE41 pKa = 4.53PQPRR45 pKa = 11.84GRR47 pKa = 11.84RR48 pKa = 11.84GAPQHH53 pKa = 6.35EE54 pKa = 4.86DD55 pKa = 2.81MGGPEE60 pKa = 4.31GYY62 pKa = 10.2GYY64 pKa = 11.01NDD66 pKa = 4.72GYY68 pKa = 10.53DD69 pKa = 2.95TGYY72 pKa = 11.2DD73 pKa = 2.99SGYY76 pKa = 10.54DD77 pKa = 3.22RR78 pKa = 11.84QGAPEE83 pKa = 4.22PAHH86 pKa = 6.61DD87 pKa = 3.89RR88 pKa = 11.84RR89 pKa = 11.84VAGGPLAAPTAQAAQGPPGPQVPQGPQAPPPPAPSGQASDD129 pKa = 4.46EE130 pKa = 4.3MADD133 pKa = 3.45DD134 pKa = 5.58DD135 pKa = 4.25YY136 pKa = 11.44WATITFDD143 pKa = 3.31KK144 pKa = 11.02PKK146 pKa = 10.48FPWQHH151 pKa = 5.87DD152 pKa = 3.68HH153 pKa = 7.38DD154 pKa = 4.54GEE156 pKa = 5.57RR157 pKa = 11.84GDD159 pKa = 4.18VDD161 pKa = 3.9PAADD165 pKa = 4.25PLNAQAPVEE174 pKa = 4.12QAPADD179 pKa = 3.61QPLPHH184 pKa = 6.38QPNLTQPVSMGADD197 pKa = 3.32GPTFGGMGAPGGQGGGTGPQQMAAPGGFQGGSPFANQDD235 pKa = 3.57PGSTALDD242 pKa = 5.0PIPADD247 pKa = 3.4LTGPGGPGPQGGPQPPYY264 pKa = 10.25GSGPGVPEE272 pKa = 3.52QPMYY276 pKa = 10.82GGQEE280 pKa = 4.13TYY282 pKa = 10.67GGQEE286 pKa = 3.76PQPAFGAADD295 pKa = 4.61FGQSDD300 pKa = 4.35QYY302 pKa = 11.72GGAPDD307 pKa = 4.27PVYY310 pKa = 10.95GDD312 pKa = 4.17PSPHH316 pKa = 5.84GTHH319 pKa = 6.19EE320 pKa = 4.15PSYY323 pKa = 11.3GSDD326 pKa = 3.4PLGGRR331 pKa = 11.84PAAAAGPPPAGRR343 pKa = 11.84QDD345 pKa = 3.74GYY347 pKa = 11.52DD348 pKa = 3.43FGLGSSGSGGGQPGGDD364 pKa = 3.43PRR366 pKa = 11.84VSDD369 pKa = 4.17PRR371 pKa = 11.84VSDD374 pKa = 3.62PRR376 pKa = 11.84VSDD379 pKa = 3.94PLGLPLGRR387 pKa = 11.84TDD389 pKa = 4.4EE390 pKa = 4.67PSAPPAPAAPAPPPPAAAAQGGAPGTDD417 pKa = 3.16TDD419 pKa = 3.73GHH421 pKa = 6.4KK422 pKa = 10.85LPTVDD427 pKa = 5.42EE428 pKa = 4.2LLQRR432 pKa = 11.84IQNDD436 pKa = 3.68RR437 pKa = 11.84QRR439 pKa = 11.84SSGSPSDD446 pKa = 3.68TGGGSYY452 pKa = 10.44GGSLNDD458 pKa = 4.35PLGDD462 pKa = 3.77PLGTGSFGTGSGTGTTGPWSTSGQTGGYY490 pKa = 10.33DD491 pKa = 2.96SGAPSGYY498 pKa = 10.2EE499 pKa = 3.64SGLGGTSGYY508 pKa = 10.93GQGQQSDD515 pKa = 5.0GYY517 pKa = 8.17PTAPAYY523 pKa = 10.42GDD525 pKa = 3.29SSRR528 pKa = 11.84YY529 pKa = 9.81DD530 pKa = 3.35DD531 pKa = 4.78PLNGTGRR538 pKa = 11.84EE539 pKa = 4.07PYY541 pKa = 9.3ATFDD545 pKa = 3.75GGSGTGTDD553 pKa = 3.68GQGVYY558 pKa = 11.09GDD560 pKa = 4.67FSGSSYY566 pKa = 11.52NGGADD571 pKa = 3.65PLSPPSDD578 pKa = 3.17QGASGGGYY586 pKa = 9.69GDD588 pKa = 4.34PGATQSYY595 pKa = 9.26GPGGYY600 pKa = 9.67GVPQPGGYY608 pKa = 8.26QQGHH612 pKa = 6.76PNDD615 pKa = 3.71TGPYY619 pKa = 9.71GSRR622 pKa = 11.84QPADD626 pKa = 2.9DD627 pKa = 3.59WEE629 pKa = 4.29NYY631 pKa = 8.27RR632 pKa = 11.84RR633 pKa = 3.78

Molecular weight: 63.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A372GPP1|A0A372GPP1_9ACTN Slipin family protein OS=Actinomadura sp. LHW52907 OX=2303421 GN=D0T12_02165 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 5.34KK15 pKa = 10.47KK16 pKa = 8.84HH17 pKa = 5.5GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSNRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.31GRR40 pKa = 11.84ARR42 pKa = 11.84IAVV45 pKa = 3.5

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6669

0

6669

2203683

27

6004

330.4

35.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.064 ± 0.044

0.801 ± 0.009

6.349 ± 0.023

5.718 ± 0.025

2.768 ± 0.016

9.338 ± 0.031

2.253 ± 0.014

3.422 ± 0.023

1.978 ± 0.023

10.318 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.807 ± 0.014

1.767 ± 0.014

6.24 ± 0.028

2.443 ± 0.02

8.85 ± 0.035

4.75 ± 0.018

5.894 ± 0.031

8.758 ± 0.028

1.486 ± 0.012

1.998 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski