Amino acid dipepetide frequency for Actinomadura sp. LHW52907

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.062AlaAla: 20.062 ± 0.173
1.083AlaCys: 1.083 ± 0.025
8.323AlaAsp: 8.323 ± 0.068
8.543AlaGlu: 8.543 ± 0.09
3.628AlaPhe: 3.628 ± 0.039
12.211AlaGly: 12.211 ± 0.098
2.719AlaHis: 2.719 ± 0.035
4.034AlaIle: 4.034 ± 0.046
2.524AlaLys: 2.524 ± 0.045
13.917AlaLeu: 13.917 ± 0.109
2.528AlaMet: 2.528 ± 0.036
1.866AlaAsn: 1.866 ± 0.031
6.529AlaPro: 6.529 ± 0.069
3.115AlaGln: 3.115 ± 0.042
10.966AlaArg: 10.966 ± 0.082
5.608AlaSer: 5.608 ± 0.053
6.687AlaThr: 6.687 ± 0.059
11.793AlaVal: 11.793 ± 0.101
1.871AlaTrp: 1.871 ± 0.031
2.634AlaTyr: 2.634 ± 0.038
0.0AlaXaa: 0.0 ± 0.0
Cys
1.024CysAla: 1.024 ± 0.024
0.095CysCys: 0.095 ± 0.007
0.544CysAsp: 0.544 ± 0.016
0.417CysGlu: 0.417 ± 0.016
0.213CysPhe: 0.213 ± 0.01
1.015CysGly: 1.015 ± 0.022
0.205CysHis: 0.205 ± 0.012
0.153CysIle: 0.153 ± 0.008
0.117CysLys: 0.117 ± 0.007
0.745CysLeu: 0.745 ± 0.02
0.13CysMet: 0.13 ± 0.008
0.131CysAsn: 0.131 ± 0.008
0.536CysPro: 0.536 ± 0.016
0.161CysGln: 0.161 ± 0.008
0.701CysArg: 0.701 ± 0.019
0.392CysSer: 0.392 ± 0.014
0.425CysThr: 0.425 ± 0.016
0.728CysVal: 0.728 ± 0.019
0.113CysTrp: 0.113 ± 0.007
0.164CysTyr: 0.164 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
8.016AspAla: 8.016 ± 0.079
0.384AspCys: 0.384 ± 0.014
4.406AspAsp: 4.406 ± 0.048
4.363AspGlu: 4.363 ± 0.054
1.57AspPhe: 1.57 ± 0.027
6.711AspGly: 6.711 ± 0.064
1.523AspHis: 1.523 ± 0.029
1.934AspIle: 1.934 ± 0.032
1.038AspLys: 1.038 ± 0.025
6.988AspLeu: 6.988 ± 0.06
0.928AspMet: 0.928 ± 0.02
0.897AspAsn: 0.897 ± 0.026
4.819AspPro: 4.819 ± 0.054
1.511AspGln: 1.511 ± 0.026
5.716AspArg: 5.716 ± 0.054
2.079AspSer: 2.079 ± 0.032
2.946AspThr: 2.946 ± 0.04
5.572AspVal: 5.572 ± 0.053
0.919AspTrp: 0.919 ± 0.022
1.164AspTyr: 1.164 ± 0.028
0.0AspXaa: 0.0 ± 0.0
Glu
7.002GluAla: 7.002 ± 0.067
0.387GluCys: 0.387 ± 0.016
2.804GluAsp: 2.804 ± 0.041
3.356GluGlu: 3.356 ± 0.047
1.602GluPhe: 1.602 ± 0.028
3.953GluGly: 3.953 ± 0.048
1.59GluHis: 1.59 ± 0.028
2.597GluIle: 2.597 ± 0.035
1.315GluLys: 1.315 ± 0.024
6.601GluLeu: 6.601 ± 0.069
1.036GluMet: 1.036 ± 0.025
1.087GluAsn: 1.087 ± 0.023
3.711GluPro: 3.711 ± 0.052
1.953GluGln: 1.953 ± 0.033
6.192GluArg: 6.192 ± 0.064
2.486GluSer: 2.486 ± 0.033
3.091GluThr: 3.091 ± 0.037
4.508GluVal: 4.508 ± 0.048
0.811GluTrp: 0.811 ± 0.021
1.097GluTyr: 1.097 ± 0.022
0.0GluXaa: 0.0 ± 0.0
Phe
3.605PheAla: 3.605 ± 0.043
0.282PheCys: 0.282 ± 0.012
2.113PheAsp: 2.113 ± 0.037
1.475PheGlu: 1.475 ± 0.028
0.834PhePhe: 0.834 ± 0.021
3.126PheGly: 3.126 ± 0.043
0.627PheHis: 0.627 ± 0.015
0.743PheIle: 0.743 ± 0.02
0.48PheLys: 0.48 ± 0.016
2.597PheLeu: 2.597 ± 0.041
0.411PheMet: 0.411 ± 0.014
0.541PheAsn: 0.541 ± 0.015
1.412PhePro: 1.412 ± 0.029
0.715PheGln: 0.715 ± 0.017
1.929PheArg: 1.929 ± 0.03
1.331PheSer: 1.331 ± 0.025
1.997PheThr: 1.997 ± 0.029
2.473PheVal: 2.473 ± 0.031
0.417PheTrp: 0.417 ± 0.013
0.575PheTyr: 0.575 ± 0.018
0.0PheXaa: 0.0 ± 0.0
Gly
10.505GlyAla: 10.505 ± 0.078
0.848GlyCys: 0.848 ± 0.022
5.84GlyAsp: 5.84 ± 0.056
5.125GlyGlu: 5.125 ± 0.049
2.864GlyPhe: 2.864 ± 0.035
8.948GlyGly: 8.948 ± 0.089
2.278GlyHis: 2.278 ± 0.042
3.191GlyIle: 3.191 ± 0.041
2.19GlyLys: 2.19 ± 0.035
9.432GlyLeu: 9.432 ± 0.084
1.928GlyMet: 1.928 ± 0.03
1.633GlyAsn: 1.633 ± 0.038
5.375GlyPro: 5.375 ± 0.058
2.361GlyGln: 2.361 ± 0.041
8.635GlyArg: 8.635 ± 0.071
4.683GlySer: 4.683 ± 0.058
5.9GlyThr: 5.9 ± 0.059
7.93GlyVal: 7.93 ± 0.068
1.635GlyTrp: 1.635 ± 0.032
2.083GlyTyr: 2.083 ± 0.032
0.0GlyXaa: 0.0 ± 0.0
His
2.687HisAla: 2.687 ± 0.038
0.217HisCys: 0.217 ± 0.011
1.483HisAsp: 1.483 ± 0.028
1.225HisGlu: 1.225 ± 0.027
0.555HisPhe: 0.555 ± 0.016
2.39HisGly: 2.39 ± 0.033
0.671HisHis: 0.671 ± 0.019
0.709HisIle: 0.709 ± 0.017
0.311HisLys: 0.311 ± 0.013
2.39HisLeu: 2.39 ± 0.035
0.364HisMet: 0.364 ± 0.013
0.378HisAsn: 0.378 ± 0.014
1.725HisPro: 1.725 ± 0.035
0.563HisGln: 0.563 ± 0.017
2.169HisArg: 2.169 ± 0.036
0.871HisSer: 0.871 ± 0.021
1.134HisThr: 1.134 ± 0.026
1.876HisVal: 1.876 ± 0.029
0.339HisTrp: 0.339 ± 0.013
0.473HisTyr: 0.473 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
4.948IleAla: 4.948 ± 0.054
0.317IleCys: 0.317 ± 0.012
2.502IleAsp: 2.502 ± 0.037
2.237IleGlu: 2.237 ± 0.035
0.816IlePhe: 0.816 ± 0.02
3.654IleGly: 3.654 ± 0.045
0.602IleHis: 0.602 ± 0.018
1.074IleIle: 1.074 ± 0.025
0.74IleLys: 0.74 ± 0.022
2.644IleLeu: 2.644 ± 0.036
0.551IleMet: 0.551 ± 0.015
0.73IleAsn: 0.73 ± 0.018
1.822IlePro: 1.822 ± 0.031
0.715IleGln: 0.715 ± 0.017
2.508IleArg: 2.508 ± 0.035
1.692IleSer: 1.692 ± 0.029
2.285IleThr: 2.285 ± 0.031
3.41IleVal: 3.41 ± 0.043
0.385IleTrp: 0.385 ± 0.013
0.593IleTyr: 0.593 ± 0.017
0.0IleXaa: 0.0 ± 0.0
Lys
2.586LysAla: 2.586 ± 0.045
0.108LysCys: 0.108 ± 0.007
1.191LysAsp: 1.191 ± 0.025
1.063LysGlu: 1.063 ± 0.028
0.446LysPhe: 0.446 ± 0.018
1.615LysGly: 1.615 ± 0.033
0.411LysHis: 0.411 ± 0.014
0.96LysIle: 0.96 ± 0.023
0.68LysLys: 0.68 ± 0.025
1.782LysLeu: 1.782 ± 0.034
0.368LysMet: 0.368 ± 0.014
0.517LysAsn: 0.517 ± 0.016
1.266LysPro: 1.266 ± 0.029
0.558LysGln: 0.558 ± 0.016
1.531LysArg: 1.531 ± 0.031
1.061LysSer: 1.061 ± 0.027
1.186LysThr: 1.186 ± 0.027
1.816LysVal: 1.816 ± 0.029
0.226LysTrp: 0.226 ± 0.01
0.406LysTyr: 0.406 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
14.791LeuAla: 14.791 ± 0.105
0.827LeuCys: 0.827 ± 0.021
7.152LeuAsp: 7.152 ± 0.065
4.876LeuGlu: 4.876 ± 0.053
2.6LeuPhe: 2.6 ± 0.038
9.136LeuGly: 9.136 ± 0.075
2.189LeuHis: 2.189 ± 0.034
3.578LeuIle: 3.578 ± 0.043
1.855LeuLys: 1.855 ± 0.035
10.945LeuLeu: 10.945 ± 0.106
1.603LeuMet: 1.603 ± 0.028
1.841LeuAsn: 1.841 ± 0.028
6.365LeuPro: 6.365 ± 0.064
1.98LeuGln: 1.98 ± 0.03
9.489LeuArg: 9.489 ± 0.079
5.039LeuSer: 5.039 ± 0.046
6.774LeuThr: 6.774 ± 0.058
9.054LeuVal: 9.054 ± 0.07
1.293LeuTrp: 1.293 ± 0.026
1.792LeuTyr: 1.792 ± 0.027
0.0LeuXaa: 0.0 ± 0.0
Met
2.347MetAla: 2.347 ± 0.033
0.129MetCys: 0.129 ± 0.008
0.981MetAsp: 0.981 ± 0.02
0.786MetGlu: 0.786 ± 0.019
0.514MetPhe: 0.514 ± 0.015
1.342MetGly: 1.342 ± 0.027
0.329MetHis: 0.329 ± 0.012
0.777MetIle: 0.777 ± 0.021
0.393MetLys: 0.393 ± 0.012
1.826MetLeu: 1.826 ± 0.028
0.317MetMet: 0.317 ± 0.014
0.434MetAsn: 0.434 ± 0.016
1.151MetPro: 1.151 ± 0.027
0.378MetGln: 0.378 ± 0.011
1.646MetArg: 1.646 ± 0.027
1.28MetSer: 1.28 ± 0.024
1.517MetThr: 1.517 ± 0.026
1.417MetVal: 1.417 ± 0.027
0.209MetTrp: 0.209 ± 0.012
0.295MetTyr: 0.295 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.263AsnAla: 2.263 ± 0.033
0.182AsnCys: 0.182 ± 0.01
1.013AsnAsp: 1.013 ± 0.02
0.883AsnGlu: 0.883 ± 0.021
0.442AsnPhe: 0.442 ± 0.015
1.935AsnGly: 1.935 ± 0.039
0.36AsnHis: 0.36 ± 0.013
0.628AsnIle: 0.628 ± 0.018
0.373AsnLys: 0.373 ± 0.017
1.76AsnLeu: 1.76 ± 0.033
0.285AsnMet: 0.285 ± 0.013
0.382AsnAsn: 0.382 ± 0.016
1.35AsnPro: 1.35 ± 0.027
0.469AsnGln: 0.469 ± 0.016
1.35AsnArg: 1.35 ± 0.027
0.761AsnSer: 0.761 ± 0.021
1.027AsnThr: 1.027 ± 0.023
1.6AsnVal: 1.6 ± 0.031
0.243AsnTrp: 0.243 ± 0.011
0.367AsnTyr: 0.367 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
7.762ProAla: 7.762 ± 0.084
0.394ProCys: 0.394 ± 0.015
5.02ProAsp: 5.02 ± 0.056
4.198ProGlu: 4.198 ± 0.046
1.589ProPhe: 1.589 ± 0.027
6.917ProGly: 6.917 ± 0.064
1.366ProHis: 1.366 ± 0.028
1.733ProIle: 1.733 ± 0.028
1.198ProLys: 1.198 ± 0.024
5.27ProLeu: 5.27 ± 0.056
1.086ProMet: 1.086 ± 0.023
1.029ProAsn: 1.029 ± 0.024
4.549ProPro: 4.549 ± 0.072
1.429ProGln: 1.429 ± 0.032
4.513ProArg: 4.513 ± 0.047
3.42ProSer: 3.42 ± 0.046
3.259ProThr: 3.259 ± 0.038
5.399ProVal: 5.399 ± 0.049
0.942ProTrp: 0.942 ± 0.021
1.322ProTyr: 1.322 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
3.293GlnAla: 3.293 ± 0.048
0.159GlnCys: 0.159 ± 0.009
1.237GlnAsp: 1.237 ± 0.024
1.291GlnGlu: 1.291 ± 0.025
0.626GlnPhe: 0.626 ± 0.016
1.973GlnGly: 1.973 ± 0.031
0.513GlnHis: 0.513 ± 0.016
1.129GlnIle: 1.129 ± 0.025
0.54GlnLys: 0.54 ± 0.015
2.403GlnLeu: 2.403 ± 0.039
0.467GlnMet: 0.467 ± 0.015
0.509GlnAsn: 0.509 ± 0.018
1.441GlnPro: 1.441 ± 0.036
0.933GlnGln: 0.933 ± 0.035
2.341GlnArg: 2.341 ± 0.037
1.087GlnSer: 1.087 ± 0.023
1.235GlnThr: 1.235 ± 0.024
2.349GlnVal: 2.349 ± 0.036
0.384GlnTrp: 0.384 ± 0.013
0.515GlnTyr: 0.515 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
10.712ArgAla: 10.712 ± 0.095
0.679ArgCys: 0.679 ± 0.018
5.387ArgAsp: 5.387 ± 0.055
5.013ArgGlu: 5.013 ± 0.06
2.644ArgPhe: 2.644 ± 0.036
6.406ArgGly: 6.406 ± 0.059
2.297ArgHis: 2.297 ± 0.033
3.197ArgIle: 3.197 ± 0.039
1.651ArgLys: 1.651 ± 0.033
10.109ArgLeu: 10.109 ± 0.077
1.905ArgMet: 1.905 ± 0.028
1.505ArgAsn: 1.505 ± 0.032
5.783ArgPro: 5.783 ± 0.059
2.221ArgGln: 2.221 ± 0.032
9.328ArgArg: 9.328 ± 0.084
4.186ArgSer: 4.186 ± 0.043
5.171ArgThr: 5.171 ± 0.049
6.918ArgVal: 6.918 ± 0.061
1.512ArgTrp: 1.512 ± 0.028
1.873ArgTyr: 1.873 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
5.965SerAla: 5.965 ± 0.062
0.385SerCys: 0.385 ± 0.014
2.623SerAsp: 2.623 ± 0.036
2.279SerGlu: 2.279 ± 0.039
1.401SerPhe: 1.401 ± 0.027
5.6SerGly: 5.6 ± 0.051
0.926SerHis: 0.926 ± 0.021
1.526SerIle: 1.526 ± 0.025
0.923SerLys: 0.923 ± 0.026
4.568SerLeu: 4.568 ± 0.055
1.096SerMet: 1.096 ± 0.023
0.81SerAsn: 0.81 ± 0.021
3.15SerPro: 3.15 ± 0.045
1.081SerGln: 1.081 ± 0.022
3.89SerArg: 3.89 ± 0.04
2.58SerSer: 2.58 ± 0.045
2.781SerThr: 2.781 ± 0.039
4.014SerVal: 4.014 ± 0.049
0.82SerTrp: 0.82 ± 0.02
1.077SerTyr: 1.077 ± 0.026
0.0SerXaa: 0.0 ± 0.0
Thr
7.865ThrAla: 7.865 ± 0.078
0.437ThrCys: 0.437 ± 0.014
3.358ThrAsp: 3.358 ± 0.045
3.103ThrGlu: 3.103 ± 0.043
1.716ThrPhe: 1.716 ± 0.028
6.511ThrGly: 6.511 ± 0.054
1.108ThrHis: 1.108 ± 0.026
2.037ThrIle: 2.037 ± 0.035
1.069ThrLys: 1.069 ± 0.028
5.748ThrLeu: 5.748 ± 0.056
1.059ThrMet: 1.059 ± 0.024
0.945ThrAsn: 0.945 ± 0.023
3.906ThrPro: 3.906 ± 0.05
1.154ThrGln: 1.154 ± 0.026
4.494ThrArg: 4.494 ± 0.047
2.986ThrSer: 2.986 ± 0.04
3.476ThrThr: 3.476 ± 0.056
5.842ThrVal: 5.842 ± 0.053
0.906ThrTrp: 0.906 ± 0.024
1.216ThrTyr: 1.216 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
11.159ValAla: 11.159 ± 0.084
0.742ValCys: 0.742 ± 0.015
5.283ValAsp: 5.283 ± 0.051
5.02ValGlu: 5.02 ± 0.057
2.509ValPhe: 2.509 ± 0.037
6.808ValGly: 6.808 ± 0.065
2.037ValHis: 2.037 ± 0.03
3.156ValIle: 3.156 ± 0.041
1.686ValLys: 1.686 ± 0.028
9.631ValLeu: 9.631 ± 0.08
1.471ValMet: 1.471 ± 0.03
1.687ValAsn: 1.687 ± 0.026
5.619ValPro: 5.619 ± 0.06
2.074ValGln: 2.074 ± 0.03
7.672ValArg: 7.672 ± 0.068
4.207ValSer: 4.207 ± 0.043
5.859ValThr: 5.859 ± 0.054
8.277ValVal: 8.277 ± 0.088
1.113ValTrp: 1.113 ± 0.028
1.567ValTyr: 1.567 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.579TrpAla: 1.579 ± 0.03
0.151TrpCys: 0.151 ± 0.009
0.836TrpAsp: 0.836 ± 0.022
0.708TrpGlu: 0.708 ± 0.019
0.492TrpPhe: 0.492 ± 0.014
1.004TrpGly: 1.004 ± 0.024
0.36TrpHis: 0.36 ± 0.015
0.556TrpIle: 0.556 ± 0.017
0.338TrpLys: 0.338 ± 0.013
1.718TrpLeu: 1.718 ± 0.031
0.265TrpMet: 0.265 ± 0.01
0.399TrpAsn: 0.399 ± 0.013
0.838TrpPro: 0.838 ± 0.018
0.469TrpGln: 0.469 ± 0.014
1.554TrpArg: 1.554 ± 0.028
0.847TrpSer: 0.847 ± 0.017
1.092TrpThr: 1.092 ± 0.024
0.947TrpVal: 0.947 ± 0.024
0.377TrpTrp: 0.377 ± 0.015
0.33TrpTyr: 0.33 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.47TyrAla: 2.47 ± 0.035
0.201TyrCys: 0.201 ± 0.01
1.388TyrAsp: 1.388 ± 0.038
1.218TyrGlu: 1.218 ± 0.026
0.622TyrPhe: 0.622 ± 0.018
2.128TyrGly: 2.128 ± 0.034
0.42TyrHis: 0.42 ± 0.014
0.512TyrIle: 0.512 ± 0.017
0.356TyrLys: 0.356 ± 0.014
2.104TyrLeu: 2.104 ± 0.032
0.27TyrMet: 0.27 ± 0.012
0.353TyrAsn: 0.353 ± 0.015
1.05TyrPro: 1.05 ± 0.024
0.584TyrGln: 0.584 ± 0.016
1.875TyrArg: 1.875 ± 0.027
0.901TyrSer: 0.901 ± 0.02
1.093TyrThr: 1.093 ± 0.027
1.657TyrVal: 1.657 ± 0.031
0.343TyrTrp: 0.343 ± 0.013
0.433TyrTyr: 0.433 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6669 proteins (2203683 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski